##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780360_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 49554 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.88061508657223 34.0 34.0 34.0 31.0 34.0 2 33.348024377446826 34.0 34.0 34.0 33.0 34.0 3 33.495681478790814 34.0 34.0 34.0 34.0 34.0 4 33.50084756023732 34.0 34.0 34.0 34.0 34.0 5 33.512329983452396 34.0 34.0 34.0 34.0 34.0 6 37.28423537958591 38.0 37.0 38.0 36.0 38.0 7 36.867639342939015 38.0 37.0 38.0 35.0 38.0 8 36.896294950962584 38.0 37.0 38.0 35.0 38.0 9 36.6258425152359 38.0 37.0 38.0 34.0 38.0 10-11 36.37408685474432 38.0 37.0 38.0 34.0 38.0 12-13 36.20480687734593 38.0 37.0 38.0 34.0 38.0 14-15 36.37640755539412 38.0 37.0 38.0 34.0 38.0 16-17 36.46721758082092 38.0 37.0 38.0 34.0 38.0 18-19 36.53408402954353 38.0 37.0 38.0 34.0 38.0 20-21 36.25547887153408 38.0 37.0 38.0 33.5 38.0 22-23 36.30618113573072 38.0 37.0 38.0 33.5 38.0 24-25 35.48600516608145 38.0 36.0 38.0 29.0 38.0 26-27 35.72344311256407 38.0 36.0 38.0 31.0 38.0 28-29 35.60248415869556 38.0 36.0 38.0 31.0 38.0 30-31 35.27142107599791 38.0 36.0 38.0 29.0 38.0 32-33 35.75072648020341 38.0 36.5 38.0 31.5 38.0 34-35 35.54555636275578 38.0 36.0 38.0 31.0 38.0 36-37 35.592313435847764 38.0 36.0 38.0 31.0 38.0 38-39 34.9611232191145 37.5 35.5 38.0 28.5 38.0 40-41 34.93350688138193 37.0 35.0 38.0 29.0 38.0 42-43 35.564787908140616 37.5 36.0 38.0 31.5 38.0 44-45 35.78884651087702 38.0 36.5 38.0 32.0 38.0 46-47 36.03471969972152 38.0 37.0 38.0 33.5 38.0 48-49 35.746286878960326 38.0 36.5 38.0 31.5 38.0 50-51 35.855248819469665 38.0 37.0 38.0 31.5 38.0 52-53 35.744874278564794 38.0 37.0 38.0 31.0 38.0 54-55 35.954393187230096 38.0 37.0 38.0 32.5 38.0 56-57 35.96687452072487 38.0 37.0 38.0 33.0 38.0 58-59 35.97074908180974 38.0 37.0 38.0 33.0 38.0 60-61 35.73651975622553 38.0 36.5 38.0 32.5 38.0 62-63 36.10705492997538 38.0 37.0 38.0 33.5 38.0 64-65 36.109103200548894 38.0 37.0 38.0 33.0 38.0 66-67 35.12377406465674 38.0 36.0 38.0 29.0 38.0 68-69 33.72167736206966 37.0 34.0 38.0 22.5 38.0 70-71 32.88066553658635 37.0 32.5 38.0 16.0 38.0 72-73 31.93467732170965 37.0 29.0 38.0 11.0 38.0 74-75 32.27850425798119 37.0 31.0 38.0 11.0 38.0 76 31.926928199539894 37.0 28.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34637249271212767 1101 2 0.5989805862510238 1101 3 0.3240905195656012 1101 4 0.3247539133210253 1101 5 0.18850150842862945 1101 6 -1.1714954902774153 1101 7 0.16307450382895183 1101 8 0.6523378459439044 1101 9 0.5056567743853719 1101 10-11 0.3845776876217215 1101 12-13 0.6913041773538353 1101 14-15 0.509070713259014 1101 16-17 0.21007257616417263 1101 18-19 -0.3729586117145942 1101 20-21 -0.0070705288395132015 1101 22-23 0.6405587572053264 1101 24-25 -2.54056254011833 1101 26-27 -0.9107126503165048 1101 28-29 0.6722636931792607 1101 30-31 0.8855655624181225 1101 32-33 -0.049862423484782425 1101 34-35 0.36955956535513224 1101 36-37 -0.18718096267410544 1101 38-39 0.7896185756322112 1101 40-41 1.2093236729354686 1101 42-43 0.7717112677382261 1101 44-45 0.45914136258303984 1101 46-47 0.7976644217146927 1101 48-49 -1.398218465757914 1101 50-51 -1.4234662201343582 1101 52-53 -1.5175977099124012 1101 54-55 -0.6889850166206912 1101 56-57 -1.6777075960467442 1101 58-59 -1.3167112072146594 1101 60-61 -2.017011361749404 1101 62-63 -0.7568822281875782 1101 64-65 -1.8715017476555147 1101 66-67 -2.555078517092973 1101 68-69 -1.9629418996010344 1101 70-71 -2.424919600631256 1101 72-73 -2.3127655330787427 1101 74-75 -1.3927267245043176 1101 76 -1.4952413976437917 1102 1 -0.23383584062120377 1102 2 -0.05362358041564619 1102 3 -0.03372198043440022 1102 4 0.22371224665435818 1102 5 -0.06097765823803769 1102 6 0.27485867638925043 1102 7 0.30890783716228754 1102 8 0.33046284594390585 1102 9 0.5811776077187076 1102 10-11 0.4069735209550558 1102 12-13 0.6160437606871696 1102 14-15 -0.07426262007431461 1102 16-17 0.21996840949750407 1102 18-19 -0.18623986171459705 1102 20-21 0.48824197116048396 1102 22-23 0.06555875720532356 1102 24-25 -5.553583373451666 1102 26-27 -1.5221709836498363 1102 28-29 -0.007684223487402164 1102 30-31 1.676450979084791 1102 32-33 -0.4826749234847796 1102 34-35 0.058101232021797955 1102 36-37 -3.5861392960074383 1102 38-39 -1.0168918410344574 1102 40-41 0.5645320062688057 1102 42-43 0.615721684404889 1102 44-45 0.689089279249707 1102 46-47 1.3492269217146955 1102 48-49 -3.832333049091247 1102 50-51 -0.8278933034676896 1102 52-53 -0.3181185432457383 1102 54-55 -0.2465370999540255 1102 56-57 0.17802157061992574 1102 58-59 -0.7195757905479994 1102 60-61 -5.957896778416071 1102 62-63 -2.6540176448542425 1102 64-65 -0.346501747655509 1102 66-67 0.9712235662403614 1102 68-69 -1.4785668996010344 1102 70-71 -2.8796071006312545 1102 72-73 -7.170057199745411 1102 74-75 -3.962778807837651 1102 76 -2.007741397643791 1103 1 1.0358652463353195 1103 2 0.46166174567130724 1103 3 0.2711919688409594 1103 4 0.30591333361088147 1103 5 0.280168175095298 1103 6 0.21292479957765664 1103 7 0.7011179820898263 1103 8 0.6635697300018748 1103 9 0.17232344105204334 1103 10-11 0.32443276008549304 1103 12-13 -0.8090581414867373 1103 14-15 -0.4184655186250339 1103 16-17 0.37927547471489476 1103 18-19 0.9315703737926526 1103 20-21 -0.055802412897485 1103 22-23 0.8101239745966211 1103 24-25 -3.0034248589589154 1103 26-27 -0.4041909111860704 1103 28-29 -0.021214567690300612 1103 30-31 1.2924496203891351 1103 32-33 1.3249564170949313 1103 34-35 0.8653928986884694 1103 36-37 -0.5043911076016414 1103 38-39 0.8003069814293085 1103 40-41 -0.024915457499311344 1103 42-43 -0.1598104713922126 1103 44-45 1.3982717973656449 1103 46-47 1.1072658709900551 1103 48-49 -2.270682233873856 1103 50-51 0.1500845045033259 1103 52-53 0.1532356234209331 1103 54-55 -0.19858646589605655 1103 56-57 0.782980809750363 1103 58-59 0.3669844449592503 1103 60-61 -3.5041490429088213 1103 62-63 -0.9262662861585866 1103 64-65 0.08465767263434998 1103 66-67 -0.06449880694804477 1103 68-69 0.3060798395294029 1103 70-71 0.6687398196586045 1103 72-73 -3.961497417136716 1103 74-75 -1.6612049853738853 1103 76 -3.2985022672090096 1104 1 0.2683311301817142 1104 2 -0.028817467276958553 1104 3 0.20401752686486674 1104 4 -0.3156353810828705 1104 5 -0.21009946480737796 1104 6 0.12163103040384726 1104 7 0.5009698809579106 1104 8 0.3390166780606947 1104 9 0.8791360931201666 1104 10-11 -1.0280743805047976 1104 12-13 0.05511196324191303 1104 14-15 0.6903359200716679 1104 16-17 0.23154459562889684 1104 18-19 0.6026800744167886 1104 20-21 -0.6235851273796627 1104 22-23 -0.2765945274662158 1104 24-25 0.2707209148695071 1104 26-27 1.232961315620237 1104 28-29 1.4796846177534775 1104 30-31 0.33307164514318544 1104 32-33 0.5046083794349272 1104 34-35 -0.4162372716278355 1104 36-37 1.1343214704159195 1104 38-39 1.5797949746589737 1104 40-41 2.0546095610133364 1104 42-43 -0.12056367143452462 1104 44-45 0.6433262774248902 1104 46-47 1.085711866970172 1104 48-49 0.021367908937946822 1104 50-51 0.32878438813814626 1104 52-53 -0.044452941055958206 1104 54-55 0.03707946026494824 1104 56-57 -0.08777450602240577 1104 58-59 -0.8140956354385125 1104 60-61 0.453457008080278 1104 62-63 0.6626737328829861 1104 64-65 0.6078778143882886 1104 66-67 0.14723291843014152 1104 68-69 2.214521604048599 1104 70-71 1.5806460927994053 1104 72-73 0.9974838586487529 1104 74-75 0.29893994216234887 1104 76 -0.46257716406714877 1105 1 0.7535564007581073 1105 2 0.022831160963662 1105 3 0.02581465749663181 1105 4 -0.43846447748357065 1105 5 -0.470406537548385 1105 6 -0.08672537533488622 1105 7 0.548994044058837 1105 8 -0.49335180922850697 1105 9 0.25623148702905496 1105 10-11 0.2179110209550501 1105 12-13 -0.3831211100024845 1105 14-15 0.37573737992568823 1105 16-17 0.1425438405319852 1105 18-19 0.48264483656126345 1105 20-21 0.4097398159880683 1105 22-23 0.3836622054811869 1105 24-25 0.4765351610310944 1105 26-27 -0.04921839744293521 1105 28-29 0.6900797851332925 1105 30-31 0.7099908497744423 1105 32-33 0.67815481789453 1105 34-35 -1.2478254921161351 1105 36-37 0.31957191088911685 1105 38-39 -0.37230671172411434 1105 40-41 0.13188114419983776 1105 42-43 0.1495848309566128 1105 44-45 0.45684251200832904 1105 46-47 0.9118885596457247 1105 48-49 -0.2505173163326262 1105 50-51 0.38544182584265485 1105 52-53 0.10553447399563964 1105 54-55 0.6875954431494193 1105 56-57 -0.0959547224835191 1105 58-59 0.44392097669338426 1105 60-61 0.8314081784804799 1105 62-63 -0.4282902741645884 1105 64-65 0.6828085971720768 1105 66-67 0.7282548162403586 1105 68-69 -1.0021660375320636 1105 70-71 -0.1313851178726324 1105 72-73 -0.2143459928488589 1105 74-75 -2.0251979888721365 1105 76 -0.22713794936792908 1106 1 -0.7071221309437874 1106 2 0.2621526292617702 1106 3 0.30473568085592007 1106 4 0.33711950471887775 1106 5 0.23715742240712245 1106 6 0.22823569251828246 1106 7 -1.3785921628377125 1106 8 0.2563701040084254 1106 9 -0.23359053744258063 1106 10-11 0.5493626338582871 1106 12-13 1.2956052526226571 1106 14-15 -0.2355529426549552 1106 16-17 0.5296962320781446 1106 18-19 0.8403478398983069 1106 20-21 0.23446172922499642 1106 22-23 0.7066877894633876 1106 24-25 -0.23478297022585792 1106 26-27 1.1164378873179075 1106 28-29 1.2305970265125978 1106 30-31 1.243361261342855 1106 32-33 0.6037666087732845 1106 34-35 0.8761455868605026 1106 36-37 1.1573082846377218 1106 38-39 0.30077448961070274 1106 40-41 1.1930602320752541 1106 42-43 0.8746682569855295 1106 44-45 1.4467757711851945 1106 46-47 1.148874099134055 1106 48-49 1.4240933621990735 1106 50-51 0.3093294787903744 1106 52-53 0.8897947632058774 1106 54-55 0.3417945532717823 1106 56-57 0.5280719738457336 1106 58-59 1.122536104613289 1106 60-61 0.9651122941645767 1106 62-63 0.7478220728876934 1106 64-65 -1.330372715397445 1106 66-67 0.012932235595201291 1106 68-69 1.9342355197537984 1106 70-71 0.741612657433258 1106 72-73 -0.5959644578099272 1106 74-75 -0.0034794126763628697 1106 76 0.5345973120336254 1107 1 0.9172058260454605 1107 2 -0.057963858193424755 1107 3 0.015757186232264075 1107 4 -0.05580164223452755 1107 5 0.0023903973175194437 1107 6 0.2271156208336933 1107 7 0.5658522816067304 1107 8 -0.06016215405609415 1107 9 -0.6540654478368495 1107 10-11 0.39568879873283436 1107 12-13 -0.026751378201716136 1107 14-15 -0.1367626200743146 1107 16-17 0.4864614650530612 1107 18-19 0.8298191660631744 1107 20-21 0.7790405822715982 1107 22-23 -0.48652457612801214 1107 24-25 0.07054857099277712 1107 26-27 0.21567623857238516 1107 28-29 0.2965692487348264 1107 30-31 0.30362111797367675 1107 32-33 1.045970909848549 1107 34-35 0.15011512091068369 1107 36-37 1.596152370659233 1107 38-39 0.020868575632206898 1107 40-41 0.1496014507132486 1107 42-43 0.4981001566271104 1107 44-45 1.1591413625830356 1107 46-47 0.594886643936924 1107 48-49 0.6108093120198674 1107 50-51 0.8800060020878675 1107 52-53 0.0699022900875903 1107 54-55 0.06379276115708876 1107 56-57 0.39937573728659714 1107 58-59 0.17495545945200064 1107 60-61 0.8107664160283719 1107 62-63 0.24311777181242178 1107 64-65 0.37572047456671953 1107 66-67 0.0810325940181329 1107 68-69 1.290530322621187 1107 70-71 -0.6131140450756973 1107 72-73 1.1997344669212602 1107 74-75 -0.17328228005987256 1107 76 0.29781415791176613 1108 1 -0.29787633357894805 1108 2 0.3192857505702662 1108 3 -0.23717708606820054 1108 4 0.3101999227106944 1108 5 0.2144404755647784 1108 6 -0.13898375318821365 1108 7 -0.25887385297856014 1108 8 -0.3444931399715898 1108 9 -0.2455169345348125 1108 10-11 0.3819955279973115 1108 12-13 1.253862862799842 1108 14-15 0.6193993517566696 1108 16-17 -0.5435659214884154 1108 18-19 0.2606681488487794 1108 20-21 1.0085984852449954 1108 22-23 -0.11225814420312474 1108 24-25 1.135787225139886 1108 26-27 -0.767989645621661 1108 28-29 -0.2817269171493706 1108 30-31 -0.3079790385208412 1108 32-33 0.7971446187687405 1108 34-35 0.7585853869513741 1108 36-37 1.435647675823553 1108 38-39 0.5104519089655426 1108 40-41 0.6646640485223259 1108 42-43 0.01619483581333725 1108 44-45 1.0229911278412587 1108 46-47 0.9439672386161035 1108 48-49 1.1797158065425606 1108 50-51 0.5660877704759741 1108 52-53 -0.16131836718940207 1108 54-55 -0.10805778657374532 1108 56-57 0.09758583118330222 1108 58-59 0.7876315157900322 1108 60-61 1.395371267358577 1108 62-63 1.3114863164133652 1108 64-65 0.7133574072740672 1108 66-67 1.2451562246910655 1108 68-69 1.4375862694130461 1108 70-71 -1.3574900231664628 1108 72-73 -0.5884110729848508 1108 74-75 -1.3559896353024428 1108 76 -1.087847031446607 1109 1 -1.1686041972645604 1109 2 0.12584538811582036 1109 3 0.39017443564951293 1109 4 0.24130402987113797 1109 5 -0.01470361977649759 1109 6 -0.20290574668766936 1109 7 0.3032260189804674 1109 8 0.3930721116781726 1109 9 0.9473817161103142 1109 10-11 0.7263026293466623 1109 12-13 0.6248123358619964 1109 14-15 0.7981150023033052 1109 16-17 0.9855387766303707 1109 18-19 0.4161148148588296 1109 20-21 0.9507091914402039 1109 22-23 0.33041889706546357 1109 24-25 0.32321951116372105 1109 26-27 0.540219750615897 1109 28-29 -0.2667805958650291 1109 30-31 0.9894699913225509 1109 32-33 0.8490012128788607 1109 34-35 -0.09323180993624192 1109 36-37 1.0164612284680814 1109 38-39 1.2936686921823295 1109 40-41 0.7934437195555191 1109 42-43 1.442398913425869 1109 44-45 1.0390947425364203 1109 46-47 1.0182063797566556 1109 48-49 0.5960705785311333 1109 50-51 -0.052079273747409616 1109 52-53 0.250554970740275 1109 54-55 -0.1771260422617189 1109 56-57 0.14289263705349242 1109 58-59 0.5770533615499005 1109 60-61 1.426199593961556 1109 62-63 0.5288112450058975 1109 64-65 1.1587430075892513 1109 66-67 0.8688142567998014 1109 68-69 0.4264811773220458 1109 70-71 -0.014167852379507906 1109 72-73 0.6091167513035423 1109 74-75 0.6136252568476621 1109 76 0.6762620988597021 1110 1 -1.5316578103181726 1110 2 -0.5548072925368572 1110 3 0.11045415592923291 1110 4 0.11717815574526469 1110 5 0.4316833266104467 1110 6 0.2656257218437972 1110 7 0.46231692807138103 1110 8 0.7258226944287571 1110 9 1.1571719259005278 1110 10-11 -0.07527079722676433 1110 12-13 0.659485995535654 1110 14-15 1.1681616223499276 1110 16-17 0.5767392428308327 1110 18-19 -0.06727679353278404 1110 20-21 0.9955809863119995 1110 22-23 -0.31701700037043423 1110 24-25 -0.47806254011833715 1110 26-27 1.4498934102895547 1110 28-29 0.8518091477247225 1110 30-31 0.022686774539337762 1110 32-33 0.8452133340909782 1110 34-35 1.5131201714157356 1110 36-37 1.889712976719828 1110 38-39 1.571057969571605 1110 40-41 -0.8365096603978586 1110 42-43 0.6451961162230759 1110 44-45 1.3182322716739492 1110 46-47 0.8745583611086332 1110 48-49 1.7904178978784557 1110 50-51 0.02426105259291944 1110 52-53 0.9297507749360818 1110 54-55 0.424272559136881 1110 56-57 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-1.5676298175330956 2116 44-45 0.023482447854355826 2116 46-47 -0.9127037953395671 2116 48-49 0.2572078908312392 2116 50-51 -0.9325747472661376 2116 52-53 -0.14327600448604727 2116 54-55 -0.9266788150703036 2116 56-57 -0.2646262006978972 2116 58-59 -2.3250445405479994 2116 60-61 -1.715557873377307 2116 62-63 0.6757728105721199 2116 64-65 -1.3135560112214009 2116 66-67 -0.048489369806148375 2116 68-69 -1.7047054654925091 2116 70-71 0.7530842753377343 2116 72-73 0.681323614208079 2116 74-75 -0.6661763369074194 2116 76 -0.54117163020193 2117 1 0.6713724927121305 2117 2 0.3739805862510224 2117 3 0.2990905195656026 2117 4 0.11642057998768962 2117 5 0.2385015084286266 2117 6 0.30350450972258614 2117 7 0.6464078371622861 2117 8 0.46067117927724155 2117 9 -0.8610098922812952 2117 10-11 -0.5154223123782842 2117 12-13 -1.2378624893128318 2117 14-15 0.31323737992568823 2117 16-17 -0.9107607571691645 2117 18-19 -0.7479586117145942 2117 20-21 -0.1320705288395132 2117 22-23 0.5155587572053264 2117 24-25 -1.3072292067850029 2117 26-27 -1.1607126503165048 2117 28-29 -1.7944029734874078 2117 30-31 0.2105655624181253 2117 32-33 -2.445695756818111 2117 34-35 0.4153928986884665 2117 36-37 -1.212180962674104 2117 38-39 0.6854519089655398 2117 40-41 0.5134903396021429 2117 42-43 0.17171126773821754 2117 44-45 -0.3241919707502916 2117 46-47 -0.9690022449519731 2117 48-49 -0.11905179909124541 2117 50-51 -0.22763288680101823 2117 52-53 0.3782356234209274 2117 54-55 -0.45565168328735695 2117 56-57 0.3972924039532586 2117 58-59 -0.7750445405480022 2117 60-61 -0.8253446950827339 2117 62-63 -0.7860488948542397 2117 64-65 -1.0048350809888404 2117 66-67 -0.4009118504263043 2117 68-69 -0.42544189960103296 2117 70-71 -0.6124196006312559 2117 72-73 1.3039011335879174 2117 74-75 1.1156066088290117 2117 76 0.804758602356209 2118 1 -0.5096352592258526 2118 2 0.2852208963285392 2118 3 -0.5956769222948637 2118 4 0.039482595491570294 2118 5 0.33830770997901993 2118 6 0.32482233917994563 2118 7 0.8500900077049209 2118 8 0.6932293188121221 2118 9 -1.653257954296798 2118 10-11 -0.30844556819223357 2118 12-13 -1.1616027993903515 2118 14-15 0.7726366047318862 2118 16-17 -2.9126018424404805 2118 18-19 -0.6538694644277712 2118 20-21 -0.15716742806431938 2118 22-23 0.799085888988273 2118 24-25 1.0626351343002725 2118 26-27 -1.8374568363630104 2118 28-29 -0.13277506651066062 2118 30-31 -0.6216049802175334 2118 32-33 0.4025600571353749 2118 34-35 0.2975634413241295 2118 36-37 -3.3142158463950366 2118 38-39 -0.24536204452282817 2118 40-41 -0.15240113326608196 2118 42-43 0.8160911126994677 2118 44-45 -2.492021428114633 2118 46-47 0.19194736745112806 2118 48-49 0.6486807590482897 2118 50-51 -0.5139700961033498 2118 52-53 -0.5076170897573675 2118 54-55 -0.41117493910131486 2118 56-57 0.762505582247833 2118 58-59 -0.4219437653542002 2118 60-61 -0.994627640819175 2118 62-63 -1.4017465692728521 2118 64-65 -0.09262577866326183 2118 66-67 -0.029109524844905366 2118 68-69 -0.762845000376231 2118 70-71 0.5127741978183593 2118 72-73 1.5534166374638936 2118 74-75 0.9888624227825034 2118 76 0.7844097651469042 2119 1 0.6880391593787962 2119 2 -0.5668240114501302 2119 3 0.034435347151806184 2119 4 -0.2574299947249514 2119 5 -0.19109619272079925 2119 6 -0.9522426167141944 2119 7 -1.1803163007687445 2119 8 -0.519213878194023 2119 9 -0.3679064440054276 2119 10-11 -0.33467518594149936 2119 12-13 -0.140017661726624 2119 14-15 -0.009607447660521018 2119 16-17 -0.40745615946801195 2119 18-19 -0.3682172324042483 2119 20-21 -0.27388087366710323 2119 22-23 -0.197372277277438 2119 24-25 0.7791213679276439 2119 26-27 0.4395747060053381 2119 28-29 0.510769440305701 2119 30-31 1.0893011946020295 2119 32-33 0.47987895582556916 2119 34-35 0.7461400251252428 2119 36-37 -0.08991084773158065 2119 38-39 -0.5197205048275606 2119 40-41 -1.1439809247656783 2119 42-43 0.2866537964738569 2119 44-45 0.12322182235315182 2119 46-47 0.09895752516297307 2119 48-49 -0.027241454263659648 2119 50-51 0.9225107913598976 2119 52-53 0.021051715374952096 2119 54-55 -0.02361145340229598 2119 56-57 -1.0830236880007647 2119 58-59 0.11030028703820705 2119 60-61 0.35123576468737383 2119 62-63 0.2251580016974799 2119 64-65 0.36039480406863333 2119 66-67 0.2817030921024255 2119 68-69 1.4874891348817272 2119 70-71 1.4703390200583968 2119 72-73 1.1753091795649375 2119 74-75 0.8291123559554521 2119 76 0.7659654989079314 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 3.0 23 21.0 24 41.0 25 88.0 26 162.0 27 325.0 28 486.0 29 834.0 30 1225.0 31 1763.0 32 2702.0 33 3964.0 34 5503.0 35 6967.0 36 9640.0 37 15829.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.13960527908948 22.339266254994552 11.339145174960649 33.181983290955316 2 16.361948581345604 17.31444484804456 44.70274851676959 21.620858053840255 3 15.05832021632966 26.677967469830893 36.075796101222906 22.18791621261654 4 11.444081204342737 22.740848367437543 34.87306776445897 30.942002663760746 5 10.612664971546192 39.35101101828308 37.169552407474676 12.866771602696048 6 22.238366226742542 38.632602817128785 16.939096742947086 22.18993421318158 7 22.553174314888807 35.88005004641401 18.66246922549138 22.904306413205795 8 19.709811518747227 35.87399604471889 22.916414416596037 21.499778019937846 9 19.074141340759574 19.72797352383259 17.829034992129795 43.368850143278046 10-11 19.76227953343827 25.2663760745853 30.298260483512934 24.673083908463493 12-13 23.025386447108207 19.166969366751424 27.725309763086734 30.082334423053638 14-15 23.157565484118336 23.2261775033297 20.17294264842394 33.44331436412802 16-17 24.293699802235942 27.446825685111193 20.208257658312146 28.051216854340723 18-19 22.53198530895589 26.049360293820882 21.532066028978488 29.886588368244745 20-21 21.28183395891351 24.109052750534772 20.78944182104371 33.81967146950801 22-23 23.1807724906163 29.478952254106634 21.607741050167494 25.732534205109577 24-25 24.288654800823345 31.20535173749849 21.28486095976107 23.2211325019171 26-27 25.443960124308834 29.130847156637202 24.98385599547968 20.44133672357428 28-29 20.096258626952416 22.925495419138716 31.26286475360213 25.715381200306737 30-31 29.22367518262905 21.28183395891351 24.977801993784556 24.51668886467288 32-33 29.166162166525407 21.538120030673607 29.38007022641966 19.91564757638132 34-35 29.58086128264116 24.800217944061025 27.727327763651772 17.89159300964604 36-37 20.043790612261372 26.863623521814585 31.329458772248458 21.763127093675585 38-39 20.61992977358034 23.435040561811356 28.74339104814949 27.201638616458812 40-41 19.10642934980022 24.815352948298823 30.694797594543328 25.38342010735763 42-43 25.059531016668686 23.005206441457805 31.754247891189408 20.1810146506841 44-45 19.578641482019616 25.301691084473504 29.362917221616826 25.75675021189006 46-47 25.072648020341447 20.715784800419744 27.792912782015577 26.41865439722323 48-49 22.369536263470156 20.584614763692134 28.643500020180007 28.402348952657707 50-51 17.552568914719295 21.07700690156193 33.11841627315656 28.252007910562217 52-53 19.030754328611213 20.91556685635872 37.32796545183032 22.72571336319974 54-55 17.70492795737983 21.239455947047663 33.61988941356904 27.43572668200347 56-57 23.513742583847925 20.81163982725915 29.694878314565926 25.979739274326995 58-59 18.1055010695403 27.53359970940792 33.05989425677039 21.30100496428139 60-61 20.103321628930058 35.347297897243415 24.571174879928968 19.978205593897567 62-63 15.626387375388465 41.708035678249985 22.31908624934415 20.346490697017394 64-65 15.617306372845785 42.74024296726803 21.272752956370827 20.369697703515357 66-67 15.464947330185252 42.20749081809743 20.69560479476934 21.631957056947975 68-69 15.321669290067403 39.74956612987852 21.58453404366953 23.34423053638455 70-71 16.078419501957463 37.249263429793764 22.240384227307583 24.431932840941194 72-73 16.524397626831338 33.89635549097954 22.906324413770836 26.672922468418292 74-75 16.926988739556847 33.831779472898255 22.700488356136738 26.54074343140816 76 17.169148807361665 33.69657343504056 22.609678330709933 26.52459942688784 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 2.0 16 4.5 17 7.0 18 8.0 19 6.5 20 11.0 21 18.0 22 16.5 23 23.5 24 36.5 25 41.0 26 57.0 27 109.0 28 181.5 29 218.0 30 251.0 31 319.5 32 446.0 33 537.0 34 653.5 35 869.5 36 1055.5 37 1142.0 38 1321.5 39 1723.0 40 2279.0 41 3330.0 42 4047.0 43 4573.5 44 5288.5 45 5076.0 46 4675.0 47 4243.5 48 3488.0 49 2901.5 50 2639.0 51 2405.5 52 1930.0 53 1516.0 54 1344.0 55 1306.5 56 1100.0 57 811.5 58 692.0 59 592.0 60 422.0 61 311.0 62 270.0 63 237.0 64 189.5 65 153.5 66 111.5 67 91.0 68 80.0 69 53.5 70 26.5 71 15.0 72 12.5 73 7.5 74 4.5 75 4.0 76 3.0 77 1.5 78 0.5 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 49554.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.20490777737418 #Duplication Level Percentage of deduplicated Percentage of total 1 69.08501825042038 33.99321951810146 2 16.35155641225444 16.091536505630224 3 6.406102612475905 9.456350647778182 4 3.03080014764385 5.965209670258707 5 1.619981134396916 3.9855511159543124 6 1.0130008612557928 2.9906768373895143 7 0.6520936718205307 2.246034628889696 8 0.4839437312881926 1.9049925333979094 9 0.3116925726940902 1.3803123864874682 >10 0.9637862445146208 6.419259797392743 >50 0.04101218061764344 1.4368164023085928 >100 0.028708526432350407 2.837308794446462 >500 0.004101218061764344 1.580094442426444 >1k 0.008202436123528688 9.712636719538281 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGT 2476 4.996569399039432 No Hit ATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTT 2337 4.7160673204988495 No Hit CTTTACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC 783 1.580094442426444 No Hit CTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC 438 0.8838842474875893 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG 308 0.6215441740323687 No Hit TATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCT 199 0.40158211244299147 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC 140 0.28252007910562216 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATATCGTATGCCGT 109 0.21996206158937723 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC 108 0.2179440610243371 No Hit CTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG 104 0.20987205876417644 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC 84 0.16951204746337328 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTACCTCGTATGCCGT 84 0.16951204746337328 No Hit CTTATAACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG 80 0.16144004520321265 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGT 78 0.15740404407313235 No Hit CTTAACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC 72 0.1452960406828914 No Hit ATACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGTCTT 68 0.13722403842273076 No Hit ATACACATCTCCGAGCCCACGAGACTGCTGGGTACCTCGTATGCCGTCTT 67 0.1352060378576906 No Hit ATACACATCTCCGAGCCCACGAGACTGCTGGGTATATCGTATGCCGTCTT 64 0.12915203616257012 No Hit TTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC 60 0.1210800339024095 No Hit CTTATTACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG 55 0.11099003107720869 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.002018000565040158 0.0 2 0.0 0.0 0.0 0.006054001695120475 0.0 3 0.0 0.0 0.0 0.006054001695120475 0.0 4 0.0 0.0 0.0 0.008072002260160633 0.0 5 0.0 0.0 0.0 0.008072002260160633 0.0 6 0.0 0.0 0.0 0.01009000282520079 0.0 7 0.0 0.0 0.0 0.01210800339024095 0.0 8 0.0 0.0 0.0 0.01210800339024095 0.0 9 0.0 0.0 0.0 0.014126003955281108 0.0 10 0.0 0.0 0.0 0.014126003955281108 0.0 11 0.0 0.0 0.0 0.016144004520321265 0.0 12 0.0 0.0 0.0 0.016144004520321265 0.0 13 0.0 0.0 0.0 0.016144004520321265 0.0 14 0.0 0.0 0.0 0.016144004520321265 0.0 15 0.0 0.0 0.0 0.02018000565040158 0.0 16 0.0 0.0 0.0 0.02219800621544174 0.0 17 0.0 0.0 0.0 0.0242160067804819 0.0 18 0.0 0.0 0.0 0.030270008475602374 0.0 19 0.0 0.0 0.0 0.030270008475602374 0.0 20 0.0 0.0 0.0 0.030270008475602374 0.0 21 0.0 0.0 0.0 0.03228800904064253 0.0 22 0.0 0.0 0.0 0.03228800904064253 0.0 23 0.0 0.0 0.0 0.03632401017072285 0.0 24 0.0 0.0 0.0 0.04237801186584332 0.0 25 0.0 0.0 0.0 0.0484320135609638 0.0 26 0.0 0.0 0.0 0.05650401582112443 0.0 27 0.0 0.0 0.0 0.07063001977640554 0.0 28 0.0 0.0 0.0 0.08879202486176696 0.0 29 0.0 0.0 0.0 0.10090002825200792 0.0 30 0.0 0.0 0.0 0.12915203616257012 0.0 31 0.0 0.0 0.0 0.16951204746337328 0.0 32 0.0 0.0 0.0 0.21390805989425676 0.0 33 0.0 0.0 0.0 0.2744480768454615 0.0 34 0.0 0.0 0.0 0.32691609153650564 0.0 35 0.0 0.0 0.0 0.4096541147031521 0.0 36 0.0 0.0 0.0 0.5529321548210033 0.0 37 0.0 0.0 0.0 0.7345522056746175 0.0 38 0.0 0.0 0.0 1.0311982887355209 0.0 39 0.0 0.0 0.0 1.3661863825321872 0.0 40 0.0 0.0 0.0 1.824272510796303 0.0 41 0.0 0.0 0.0 2.3691326633571457 0.0 42 0.0 0.0 0.0 2.976550833434233 0.0 43 0.0 0.0 0.0 3.6303830165072446 0.0 44 0.0 0.0 0.0 4.306413205795698 0.0 45 0.0 0.0 0.0 4.829075352141099 0.0 46 0.0 0.0 0.0 5.394115510352343 0.0 47 0.0 0.0 0.0 5.87641764539694 0.0 48 0.0 0.0 0.0 6.336521774226097 0.0 49 0.0 0.0 0.0 6.830931912660936 0.0 50 0.0 0.0 0.0 7.216370020583605 0.0 51 0.0 0.0 0.0 7.708762158453404 0.0 52 0.0 0.0 0.0 8.4170803567825 0.0 53 0.0 0.0 0.0 9.266658594664406 0.0 54 0.0 0.0 0.0 9.989102796948783 0.0 55 0.0 0.0 0.0 10.604592969286031 0.0 56 0.0 0.0 0.0 11.207975138233039 0.0 57 0.0 0.0 0.0 11.730637284578439 0.0 58 0.0 0.0 0.0 12.25531743148888 0.0 59 0.0 0.0 0.0 12.753763571053799 0.0 60 0.0 0.0 0.0 13.244137708358558 0.0 61 0.0 0.0 0.0 13.746619849053557 0.0 62 0.0 0.0 0.0 14.295516002744481 0.0 63 0.0 0.0 0.0 14.963474189772773 0.0 64 0.0 0.0 0.0 15.56080235702466 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTTCTC 15 0.0022123272 70.0 44 TATAACA 30 1.0041913E-7 70.0 3 CTATACA 100 0.0 63.000004 1 ATAACAC 35 2.9178227E-7 60.0 4 ATTACAC 20 0.0069130394 52.5 4 TAGTTAG 20 0.0069130394 52.5 7 TTATAAC 40 7.3386946E-7 52.5 2 CTTATAA 40 7.3386946E-7 52.5 1 AGTCTTC 20 0.0069130394 52.5 45 GATATGG 20 0.0069130394 52.5 66 GTAGTTA 20 0.0069130394 52.5 6 TTTACAC 155 0.0 49.677418 2 CTTTACA 160 0.0 48.125 1 TAACACA 45 1.653123E-6 46.666664 5 CCAGTCC 30 5.824807E-4 46.666664 4 TTACACA 175 0.0 42.0 3 ATATCGT 45 9.075055E-5 38.888885 37 GGGTACC 40 0.002397236 35.0 30 ATCGTAT 60 1.1932647E-5 35.0 39 TATATCG 45 0.004269173 31.111109 36 >>END_MODULE