FastQCFastQC Report
Wed 25 May 2016
SRR1780359_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780359_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569531
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAT702.7284841E-1134.6453917
CGACGAT300.00574428329.33145722
TGCGACA905.002221E-1026.9487915
CATCGTT801.3214958E-724.72232832
ATAAAGG1102.055458E-1023.9605354
CGGTGGT2050.023.57714543
TCGTTGT752.0433636E-623.48995634
CGGAATA500.00254566222.04900721
AAGTCGT500.00254566222.04900719
AGCTCGA500.002546965622.04706617
CGTTCGG500.002571835622.01020624
TCGTAAC500.002579730721.99859222
CTTATAA703.22619E-521.9869921
TCGGTGG2600.021.96961242
ATGCGAC1158.576535E-921.07920814
GCGACAA1301.5752448E-920.3529316
TCGTTCG550.00448240119.9987223
GGTATGA1101.280041E-719.97412941
TACTCCG901.0147505E-519.52345
TCACGTA809.012696E-519.24030925
Produced by FastQC (version 0.11.5)