FastQCFastQC Report
Wed 25 May 2016
SRR1780359_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780359_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569531
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT342426.012315396352438No Hit
ATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCTT286025.0220268958142755Illumina PCR Primer Index 6 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC82541.4492626389081542No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG46540.817163596011455No Hit
CTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC45030.7906505528232879No Hit
TATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCT25470.44721007284941466No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC19950.35028821960525414No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC14060.2468697928646553No Hit
CTTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG12750.2238684110259143No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC9430.16557483262544095No Hit
CTTATAACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG9290.16311666968084265No Hit
CTTAACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC6930.1216790657576146No Hit
TTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC5950.10447192514542669No Hit
CTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG5870.10306726060565624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT27800.042.17783744
CTTATAA1300.038.9656831
GCCGTCT31050.037.7630943
CTTTACA10750.035.443411
CTATACA6800.035.303311
TTGGCGA250.00235230435.19831519
GCGTCAC250.00235230435.19831536
TTTACAC11000.034.8075032
TCTTTAT1950.031.6243251
ATTAGAG353.2166456E-431.42706518
TTACACA12400.030.7001653
TTATACA52700.030.1051622
CTTATAC53000.030.002621
ATCAGGT300.005743838429.33192811
TATACAC60300.026.6392383
CGTAGCC508.336541E-526.39873536
GGCGCTA858.220013E-925.88111318
TGCCGTC47050.024.92122842
CTTTATA2500.024.6453112
GCTCTAT450.001388427324.4712031
Produced by FastQC (version 0.11.5)