FastQCFastQC Report
Wed 25 May 2016
SRR1780358_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780358_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70394
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAACC150.00221701269.9936119
GTAACCT150.00221701269.9936120
GCTATTC200.006927577352.49520550
GGGTAAC200.006927577352.49520518
TGCTCGC200.006927577352.49520526
CTCTGGG200.006927577352.49520525
CCTTGGG200.006927577352.49520514
GCCGTAT200.006927577352.49520554
CCTCTGG200.006927577352.49520524
CTTCCGA352.0684818E-549.9954318
ACGCTTC352.0684818E-549.9954315
TCGCCCG305.844271E-446.66240310
GCGTGAA305.844271E-446.66240359
GGCCTCG305.844271E-446.6624036
AGCGGAT404.553207E-543.74600669
GATCTCG404.553207E-543.74600641
TTGGCGT350.001248312439.9963456
GGCAGAT350.001248312439.9963430
GGAAGAG350.001248312439.9963424
ATTGGCG350.001248312439.9963455
Produced by FastQC (version 0.11.5)