##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780358_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 70394 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.39787481887661 34.0 23.0 34.0 12.0 34.0 2 29.244992470949228 34.0 27.0 34.0 12.0 34.0 3 29.605477739580078 34.0 31.0 34.0 11.0 34.0 4 29.922408159786347 34.0 31.0 34.0 11.0 34.0 5 29.91065999943177 34.0 31.0 34.0 11.0 34.0 6 32.196522430889 37.0 31.0 38.0 11.0 38.0 7 31.503338352700514 37.0 27.0 38.0 11.0 38.0 8 30.707517686166433 36.0 26.0 38.0 11.0 38.0 9 31.428559252208995 37.0 27.0 38.0 12.0 38.0 10-11 31.654594141546156 37.0 27.5 38.0 11.0 38.0 12-13 32.029896013864814 37.0 29.5 38.0 11.0 38.0 14-15 31.616813933005655 37.0 27.0 38.0 11.0 38.0 16-17 31.38770349745717 37.0 27.0 38.0 11.0 38.0 18-19 31.4421967781345 37.0 27.5 38.0 11.0 38.0 20-21 31.554990482143367 37.0 27.0 38.0 11.0 38.0 22-23 31.402221780265364 37.0 27.0 38.0 11.0 38.0 24-25 31.756222121203514 37.0 29.0 38.0 11.0 38.0 26-27 31.80475608716652 37.0 29.0 38.0 11.0 38.0 28-29 31.826341733670482 37.0 29.5 38.0 11.0 38.0 30-31 31.681173111344716 37.0 27.0 38.0 11.0 38.0 32-33 31.562171491888513 37.0 27.0 38.0 11.0 38.0 34-35 31.456317299769868 37.0 27.0 38.0 11.0 38.0 36-37 31.42029860499474 37.0 27.0 38.0 11.0 38.0 38-39 31.798590788987696 37.0 27.5 38.0 11.0 38.0 40-41 31.444846151660656 37.0 27.0 38.0 11.0 38.0 42-43 31.71893201125096 37.0 27.0 38.0 11.0 38.0 44-45 30.86549279768162 37.0 26.0 38.0 11.0 38.0 46-47 31.504808648464355 37.0 27.0 38.0 11.0 38.0 48-49 31.367410574764897 37.0 27.0 38.0 11.0 38.0 50-51 31.284519987498932 37.0 26.5 38.0 11.0 38.0 52-53 31.318578287922264 37.0 27.0 38.0 11.0 38.0 54-55 31.071511776571867 37.0 26.5 38.0 11.0 38.0 56-57 31.04535187658039 37.0 26.5 38.0 11.0 38.0 58-59 30.930611983975908 36.5 26.5 38.0 11.0 38.0 60-61 31.002741710941272 37.0 26.0 38.0 11.0 38.0 62-63 30.97242662726937 37.0 26.0 38.0 11.0 38.0 64-65 31.06773304543001 37.0 26.5 38.0 11.0 38.0 66-67 31.08315339375515 37.0 26.5 38.0 11.0 38.0 68-69 30.604582776941218 36.0 25.0 38.0 11.0 38.0 70-71 30.55302298491349 36.0 25.0 38.0 11.0 38.0 72-73 30.657144074779097 36.0 25.0 38.0 11.0 38.0 74-75 30.340881325112935 36.0 25.0 38.0 11.0 38.0 76 29.635863852032845 34.0 24.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.14131825421617 1101 2 -0.7992163980545897 1101 3 0.17588312477066737 1101 4 -1.9988407583256134 1101 5 -0.6081080886188204 1101 6 -1.9559980987452619 1101 7 0.002037248980322204 1101 8 0.08487464796834132 1101 9 -0.6876055258589524 1101 10-11 -1.2754612844716995 1101 12-13 -1.276882964512069 1101 14-15 -0.7158119733059358 1101 16-17 -2.3889975192188864 1101 18-19 -0.7418087916045302 1101 20-21 -0.724123914854033 1101 22-23 -0.47433323808257555 1101 24-25 -0.808637140283107 1101 26-27 0.053755861413183226 1101 28-29 -0.0689816164749999 1101 30-31 -1.0280554089087133 1101 32-33 0.09777878883556923 1101 34-35 -0.4148515575598388 1101 36-37 0.855595625693212 1101 38-39 -0.8083326936710833 1101 40-41 0.17327704995344817 1101 42-43 -0.4248141878379137 1101 44-45 -0.7075195535956489 1101 46-47 0.2774066678521194 1101 48-49 -1.8717812857094103 1101 50-51 -0.6900659839305163 1101 52-53 -0.5628186597168927 1101 54-55 -1.6679420692594498 1101 56-57 -0.7811225693344461 1101 58-59 -0.8822404917801236 1101 60-61 -0.2964734875916406 1101 62-63 -2.0190135020848317 1101 64-65 -0.4019316434490925 1101 66-67 -0.7571171302584574 1101 68-69 0.7639542029542277 1101 70-71 -0.22545462510297298 1101 72-73 -0.724208915233902 1101 74-75 -0.769722671427008 1101 76 -1.320964378818168 1102 1 0.25112301238240065 1102 2 -0.7328902148744731 1102 3 -0.30447097805534895 1102 4 -0.02573895376313473 1102 5 0.3876699161479493 1102 6 -0.2474860115814863 1102 7 0.10370561806442424 1102 8 1.1528692002325585 1102 9 0.8379307356323658 1102 10-11 -0.8535171237634955 1102 12-13 -4.174516604280541 1102 14-15 -1.8024479964588132 1102 16-17 -2.6122184930016594 1102 18-19 -1.6987376305558435 1102 20-21 -0.2530990595595135 1102 22-23 -1.2907615663086567 1102 24-25 0.007467728630750514 1102 26-27 -5.501385439233733 1102 28-29 -0.6541365364613867 1102 30-31 -0.4470373632839255 1102 32-33 -5.790389410006785 1102 34-35 -0.3729550645397488 1102 36-37 0.4467600792172135 1102 38-39 0.023911092505287 1102 40-41 0.014475551826105715 1102 42-43 -0.3965029859312068 1102 44-45 -5.575871613520743 1102 46-47 -1.0938054533600052 1102 48-49 -0.7151759060703249 1102 50-51 -5.091172555261807 1102 52-53 -1.7462200897475384 1102 54-55 0.35202728722676113 1102 56-57 -1.5495257017825246 1102 58-59 0.3212494639570238 1102 60-61 -0.2561091702610305 1102 62-63 -4.649912378489326 1102 64-65 -0.9081454670786435 1102 66-67 -0.3091430070034349 1102 68-69 -6.532453696262664 1102 70-71 -1.4814471344662685 1102 72-73 -0.7998813701198344 1102 74-75 -5.966011401423604 1102 76 -1.625152325702743 1103 1 1.4053719487847118 1103 2 0.3131990182443225 1103 3 0.24249095060179116 1103 4 0.4939512393207508 1103 5 0.740729804879269 1103 6 -1.3802993638232266 1103 7 0.4676742010250443 1103 8 1.0143833269915845 1103 9 0.5948304847324017 1103 10-11 0.9320997628951666 1103 12-13 -0.6683804637765576 1103 14-15 0.2538408657643849 1103 16-17 -0.036893959348336836 1103 18-19 0.4248578750621341 1103 20-21 0.127832542927667 1103 22-23 0.4172672326441038 1103 24-25 0.8347285555097734 1103 26-27 -0.2350496696253579 1103 28-29 1.3339457150931082 1103 30-31 0.931903402506407 1103 32-33 -4.451456280302413 1103 34-35 -0.04302160757454487 1103 36-37 0.4668048048635569 1103 38-39 1.2247946967378596 1103 40-41 0.915833684257656 1103 42-43 1.3913229113088903 1103 44-45 -3.1402056377380454 1103 46-47 0.6100780417856271 1103 48-49 1.3518580199687307 1103 50-51 0.8899281319859327 1103 52-53 0.663483193769423 1103 54-55 1.0821461919938606 1103 56-57 1.615141037608769 1103 58-59 1.357329970120432 1103 60-61 1.3637501075834066 1103 62-63 0.8664233911190493 1103 64-65 1.2278859499607329 1103 66-67 0.6192141436456353 1103 68-69 -2.0733067561513927 1103 70-71 1.2811796791041452 1103 72-73 2.1849555449014346 1103 74-75 -0.4174131686320699 1103 76 0.46358990185261817 1104 1 -1.3457528440166122 1104 2 0.5704805716423813 1104 3 0.5983517130929137 1104 4 0.7015287908244225 1104 5 -0.3285811115308661 1104 6 -0.8634629915760073 1104 7 1.3513351055952505 1104 8 2.125820693792434 1104 9 -0.10756117996094616 1104 10-11 0.40399178278624603 1104 12-13 0.09022716113463503 1104 14-15 0.6512242425107679 1104 16-17 -0.1637942671863648 1104 18-19 1.0907115335987179 1104 20-21 1.0735109705857333 1104 22-23 1.0395618099588084 1104 24-25 0.5433953800420959 1104 26-27 0.9295134371707583 1104 28-29 -0.03232234079133889 1104 30-31 -0.5999696735059068 1104 32-33 -1.0300852170772146 1104 34-35 0.5997086789516217 1104 36-37 0.8070368673783577 1104 38-39 0.019087853261659404 1104 40-41 0.7627079442623881 1104 42-43 0.7465087981192227 1104 44-45 -2.5278447568476814 1104 46-47 0.06595064420097074 1104 48-49 1.1177235184295355 1104 50-51 1.4752703057960161 1104 52-53 0.581527238287542 1104 54-55 0.1568916336230366 1104 56-57 0.5586409192095267 1104 58-59 0.6141379265495175 1104 60-61 0.3866011613366638 1104 62-63 0.7395230832810213 1104 64-65 -0.0228481253411843 1104 66-67 0.8361570752108705 1104 68-69 -3.1110642745032777 1104 70-71 -1.0140725112818352 1104 72-73 0.5633003234948504 1104 74-75 -0.06055785250609347 1104 76 0.07460103138138763 1105 1 -0.5997598126577266 1105 2 -0.7295194283576194 1105 3 -0.0803939315063893 1105 4 -0.1451611046459611 1105 5 -0.37910375961449105 1105 6 -0.02699376974093326 1105 7 0.9790935260366034 1105 8 1.0363897994834943 1105 9 0.5994074611540334 1105 10-11 0.7823309233205045 1105 12-13 -0.7781816658107701 1105 14-15 0.06167720418324407 1105 16-17 1.2657643855430187 1105 18-19 0.846286446490705 1105 20-21 0.387564396834275 1105 22-23 0.7897360259866879 1105 24-25 0.7366009549549908 1105 26-27 0.5295134371707597 1105 28-29 -0.11205520954859693 1105 30-31 -0.41983030068360705 1105 32-33 0.22938052043730295 1105 34-35 0.3425077497994735 1105 36-37 0.4382796083771865 1105 38-39 0.13755475221636715 1105 40-41 1.066134192810594 1105 42-43 0.42345421043048503 1105 44-45 1.6329566368805395 1105 46-47 0.40619454663999477 1105 48-49 0.0518117878836577 1105 50-51 0.33287773901320605 1105 52-53 -0.195069741967977 1105 54-55 -0.6430502943676721 1105 56-57 -0.06423945245133211 1105 58-59 -2.0585974549192088E-4 1105 60-61 -0.28262067373882616 1105 62-63 0.3452722122008822 1105 64-65 0.5188475773301313 1105 66-67 -0.08027730341862593 1105 68-69 1.1574606964607206 1105 70-71 1.2507358510875015 1105 72-73 0.35999021896523686 1105 74-75 -0.837038688743025 1105 76 -0.08892974678353482 1106 1 -0.4584899713878876 1106 2 -0.8239638728020644 1106 3 -1.00896536007782 1106 4 -0.19833570782056498 1106 5 0.016134335623604557 1106 6 -0.28731123005839265 1106 7 -0.0883667914237165 1106 8 0.9697231328168279 1106 9 0.15734396909054382 1106 10-11 -0.11131987033028778 1106 12-13 -0.13016579279489804 1106 14-15 -0.654195811689771 1106 16-17 -0.08066418588554924 1106 18-19 -0.6668087916045344 1106 20-21 0.09431042858031091 1106 22-23 -0.2670100057593423 1106 24-25 0.08858508193911163 1106 26-27 -0.3843754517181317 1106 28-29 -0.2533250508184359 1106 30-31 0.07421731836401335 1106 32-33 0.36747575853254233 1106 34-35 0.4361585434502615 1106 36-37 0.19978754488512607 1106 38-39 0.26691983158143984 1106 40-41 -0.3961673944909947 1106 42-43 0.019882781859056564 1106 44-45 0.35914711307101754 1106 46-47 0.03119454663999477 1106 48-49 0.079986391058263 1106 50-51 -0.05243972130425334 1106 52-53 -0.3661014879997211 1106 54-55 -0.1767804530978303 1106 56-57 -0.019001357213237924 1106 58-59 -0.27718998672961703 1106 60-61 0.27095075483260445 1106 62-63 0.3004309423596112 1106 64-65 0.5117047201872715 1106 66-67 0.93202428388296 1106 68-69 0.9030956170956443 1106 70-71 0.274545374897027 1106 72-73 -0.3729462889712707 1106 74-75 -1.5644196411239797 1106 76 -0.05480276265655348 1107 1 0.09156194969207121 1107 2 0.2013216931377073 1107 3 0.5620626773053594 1107 4 -0.04075523014662963 1107 5 -0.4897846674916586 1107 6 1.3271228301700617 1107 7 -0.17346544147563847 1107 8 0.5388820113215012 1107 9 -0.7373081596737343 1107 10-11 -0.8034278661766017 1107 12-13 -0.059111794871739676 1107 14-15 -0.45040557285280514 1107 16-17 0.2159869044571252 1107 18-19 0.8267270339406387 1107 20-21 0.2497361814359671 1107 22-23 -0.3306912103283999 1107 24-25 -0.4045872960463477 1107 26-27 0.1697003530586123 1107 28-29 -0.5764299313999572 1107 30-31 -0.3588823701095052 1107 32-33 0.1791580015231986 1107 34-35 -0.010674270672272002 1107 36-37 -0.18030072095080385 1107 38-39 -0.34362014765012816 1107 40-41 0.3696944954363133 1107 42-43 0.28616004042603294 1107 44-45 0.04543994796198092 1107 46-47 0.221615107387656 1107 48-49 -0.2650551458057038 1107 50-51 0.8153432485815202 1107 52-53 0.40370640400443136 1107 54-55 -0.17524878123905552 1107 56-57 0.286450356182403 1107 58-59 0.2537549769256273 1107 60-61 -0.4468841361331286 1107 62-63 -0.6166770534867041 1107 64-65 -0.23845104305260278 1107 66-67 -0.07137654685432082 1107 68-69 0.28847983308733305 1107 70-71 -0.2248315721434757 1107 72-73 0.22941612016683877 1107 74-75 -0.25032306791525727 1107 76 -0.0834112777136653 1108 1 -0.7756616885596053 1108 2 -0.5204285192667086 1108 3 -0.8200764711889299 1108 4 -0.33722459670945426 1108 5 -0.5707343512450826 1108 6 -0.4277152704624321 1108 7 0.04951199645507032 1108 8 -0.18381222071852577 1108 9 -1.1168984551518797 1108 10-11 -0.5961683551787722 1108 12-13 -0.2713274089565161 1108 14-15 0.2271173196233569 1108 16-17 -1.3884924687138351 1108 18-19 -0.20645525625099737 1108 20-21 0.3622397215095994 1108 22-23 -0.8374645512138876 1108 24-25 -1.0556068372528031 1108 26-27 -1.1119007042433822 1108 28-29 -0.7250422225356061 1108 30-31 -0.6578533887066946 1108 32-33 -0.7592919182351388 1108 34-35 -1.0527303454386256 1108 36-37 -0.8540003339027535 1108 38-39 -0.11035289569128537 1108 40-41 -0.19844012176372416 1108 42-43 -0.6212788343025579 1108 44-45 -0.8484286445047395 1108 46-47 -0.048350907905458484 1108 48-49 -0.5091550230831494 1108 50-51 -0.16077305463758762 1108 52-53 -0.3461519930502277 1108 54-55 0.4234720721546914 1108 56-57 -0.3831427713546489 1108 58-59 -1.4120384715781036 1108 60-61 -0.13637247749063164 1108 62-63 -1.0180034010747327 1108 64-65 -0.4095074010248503 1108 66-67 -0.8288343019756255 1108 68-69 -0.1057427667427433 1108 70-71 0.7548484052000539 1108 72-73 -0.4514816425066286 1108 74-75 0.2126005608962238 1108 76 0.34772248986870125 1109 1 -0.7049546178525326 1109 2 -1.7644476580226893 1109 3 0.02309205619012289 1109 4 0.19892638680995134 1109 5 0.20784087469856516 1109 6 -0.2456312725889589 1109 7 -1.606256211944718 1109 8 -0.4522864365611632 1109 9 0.7382317947152082 1109 10-11 0.679881618239623 1109 12-13 0.45342006579096505 1109 14-15 -0.2623829461926981 1109 16-17 -0.39792893867662116 1109 18-19 0.14256122434442986 1109 20-21 0.7326543945558548 1109 22-23 0.2798932371965286 1109 24-25 0.7613788086802096 1109 26-27 0.3668340113334416 1109 28-29 -0.047610005629707075 1109 30-31 0.17401795632255102 1109 32-33 1.023935088676076 1109 34-35 0.24511654451352882 1109 36-37 0.4273659606214366 1109 38-39 0.41622870983768934 1109 40-41 0.18699315840640196 1109 42-43 0.13084769413976005 1109 44-45 0.2516511003118431 1109 46-47 0.10595531219023258 1109 48-49 -0.038128973109731845 1109 50-51 0.6555480511572043 1109 52-53 0.667803349852484 1109 54-55 0.256938313515672 1109 56-57 0.6904882759606075 1109 58-59 0.4246175624463504 1109 60-61 0.8993797819458393 1109 62-63 0.09151281370463948 1109 64-65 1.0613857409209224 1109 66-67 0.7750997756426656 1109 68-69 0.8535873767979254 1109 70-71 0.6093141149289245 1109 72-73 -0.21782932990694803 1109 74-75 1.4535628150163724 1109 76 0.034059544626806826 1110 1 0.23412201232357432 1110 2 -0.3876346116036906 1110 3 -0.25346797787886644 1110 4 -0.5411222116018344 1110 5 0.9180540563914938 1110 6 0.2808097705233408 1110 7 0.7046233191056608 1110 8 1.8189377925026875 1110 9 -0.07456411700602317 1110 10-11 -1.4366543671307532 1110 12-13 0.20972658649538545 1110 14-15 -0.36158382797831123 1110 16-17 0.04049637257866934 1110 18-19 0.09239714207783933 1110 20-21 -0.8016901531532561 1110 22-23 -0.2709221639908783 1110 24-25 -0.3267872275431394 1110 26-27 -1.1197012225151006 1110 28-29 0.17805947506870723 1110 30-31 -0.14929926104262137 1110 32-33 0.5863239260718061 1110 34-35 0.15538483780744627 1110 36-37 -0.257207801246345 1110 38-39 -0.17074741681879146 1110 40-41 0.047448079621858597 1110 42-43 0.06774491100390634 1110 44-45 1.577122680261244 1110 46-47 0.3368228188913065 1110 48-49 0.8860800501623984 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-0.42506983250124364 2116 40-41 -1.3619942678760104 2116 42-43 -0.7880629545750537 2116 44-45 0.7938370355516362 2116 46-47 -0.5850845231274491 2116 48-49 -0.3220807924042681 2116 50-51 -0.49475238280296097 2116 52-53 0.1156168582535102 2116 54-55 -0.6302042257076508 2116 56-57 -1.0116370161279633 2116 58-59 -1.7505104001663128 2116 60-61 -1.907925214159647 2116 62-63 0.267614404891912 2116 64-65 -0.08965186896001498 2116 66-67 -1.2029240365304759 2116 68-69 -0.3591782847131455 2116 70-71 -2.660144547583595 2116 72-73 -1.1140961597723091 2116 74-75 -1.779148323294521 2116 76 -1.0553195585221857 2117 1 1.362833085773321 2117 2 1.6398447334920938 2117 3 1.4413236572632222 2117 4 0.9867959556347969 2117 5 0.13443876722283576 2117 6 0.14223276480352354 2117 7 0.04551246355380556 2117 8 -0.2117797573565845 2117 9 1.775873191955025 2117 10-11 1.3069524482310477 2117 12-13 1.7592529612192322 2117 14-15 0.8596127946043843 2117 16-17 1.6077269509159855 2117 18-19 1.190753828819357 2117 20-21 1.3851421562617539 2117 22-23 2.066532062320295 2117 24-25 1.7892433992159305 2117 26-27 1.887894940060935 2117 28-29 1.4853711598430905 2117 30-31 1.5381383202907912 2117 32-33 2.0200769146019084 2117 34-35 0.9198130071625314 2117 36-37 0.49090828990760826 2117 38-39 1.4553912509777192 2117 40-41 0.1071884179688638 2117 42-43 0.45971900536579824 2117 44-45 2.033906265286813 2117 46-47 0.419200326986811 2117 48-49 0.8523210089131155 2117 50-51 2.2560541772185445 2117 52-53 1.9846852814286677 2117 54-55 0.03100695859131619 2117 56-57 0.26991964471354635 2117 58-59 0.1192133530263213 2117 60-61 1.3440239725590004 2117 62-63 0.6700038389556298 2117 64-65 0.7092962423452711 2117 66-67 0.34822531150017255 2117 68-69 0.9155394192793267 2117 70-71 1.7963180145887385 2117 72-73 2.459536048857892 2117 74-75 1.3289811295889287 2117 76 0.8999499669516702 2118 1 -0.041823304721219756 2118 2 0.7871472383090463 2118 3 0.6632568621444044 2118 4 0.7961087366238786 2118 5 -0.0560878865986183 2118 6 1.4515776588304945 2118 7 1.5005220974651685 2118 8 -2.3358324227387293 2118 9 -0.6204338086872347 2118 10-11 0.2247912407808279 2118 12-13 1.0420564294273191 2118 14-15 -0.17364025613421674 2118 16-17 0.4776691474477808 2118 18-19 -0.20014212493786587 2118 20-21 -1.716800682530799 2118 22-23 0.21632332757398842 2118 24-25 0.7024739708280059 2118 26-27 0.9878467705040954 2118 28-29 0.593897171403782 2118 30-31 -0.07300490385820879 2118 32-33 0.6424757585325338 2118 34-35 0.9000474323391501 2118 36-37 0.5136764337740161 2118 38-39 1.0585864982481112 2118 40-41 -0.3489451722687704 2118 42-43 0.15877167074794585 2118 44-45 0.8202582241821261 2118 46-47 0.0867501021955519 2118 48-49 -0.45334694227506844 2118 50-51 0.30589361202908094 2118 52-53 -1.1105459324441647 2118 54-55 0.9176639913466111 2118 56-57 1.4726653094534363 2118 58-59 1.4728100132703759 2118 60-61 -0.298493689611842 2118 62-63 0.9920976090262812 2118 64-65 0.6367047201872715 2118 66-67 -1.9707534938948186 2118 68-69 1.4003178393178644 2118 70-71 -0.47823240288075297 2118 72-73 -0.8951685111934964 2118 74-75 0.7855803588760217 2118 76 0.4285305706767808 2119 1 0.5263053502495403 2119 2 1.3950419751511518 2119 3 1.238403060974811 2119 4 1.2387987951034134 2119 5 1.2067776104774062 2119 6 1.6857881851462864 2119 7 -0.419653341131319 2119 8 1.4679687468519163 2119 9 0.24009250710223995 2119 10-11 0.8492064454591919 2119 12-13 0.8960037978483797 2119 14-15 0.11481003626344233 2119 16-17 0.05661651586883565 2119 18-19 0.21725553588084878 2119 20-21 -0.3912158872091638 2119 22-23 1.4375221579833486 2119 24-25 0.7627078889566583 2119 26-27 1.0236654839543853 2119 28-29 1.1557392766669423 2119 30-31 1.0565272598844828 2119 32-33 0.8750780977138319 2119 34-35 1.1300181925730683 2119 36-37 0.222155965937759 2119 38-39 1.1607794807042424 2119 40-41 0.8387741259768404 2119 42-43 0.12792371753157283 2119 44-45 -0.8613207231862923 2119 46-47 0.700054195762803 2119 48-49 -0.22074460309378452 2119 50-51 1.1228526763565654 2119 52-53 0.11650085118156284 2119 54-55 -0.26698513146040526 2119 56-57 1.2912325609154216 2119 58-59 2.0421082588844186 2119 60-61 1.182208064774123 2119 62-63 0.4073022873888519 2119 64-65 0.8067339599533518 2119 66-67 0.5786617107835426 2119 68-69 0.1552885995517812 2119 70-71 -0.13948971282227163 2119 72-73 -1.3464843006671785 2119 74-75 0.5383581366537982 2119 76 0.9197586408522191 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 10.0 13 228.0 14 703.0 15 1151.0 16 1677.0 17 2020.0 18 2164.0 19 2004.0 20 1749.0 21 1412.0 22 1142.0 23 890.0 24 873.0 25 902.0 26 951.0 27 1033.0 28 1232.0 29 1513.0 30 1723.0 31 2215.0 32 2848.0 33 3659.0 34 5109.0 35 7377.0 36 12167.0 37 13640.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.9868310050474 28.900295980668318 10.16772237871227 27.945150635572013 2 16.323834417706053 22.109838906725006 33.06247691564622 28.503849759922723 3 14.63903173565929 30.960025002130863 27.96545160098872 26.43549166122113 4 10.61169986078359 26.49089411029349 34.27138676591755 28.62601926300537 5 10.186947751228798 41.199534051197546 32.647668835412105 15.965849362161547 6 21.676563343466775 39.06156774725119 17.034122226326108 22.227746682955935 7 23.75770662272353 32.67608034775691 21.10975367218797 22.45645935733159 8 21.63252549933233 36.63096286615337 18.412080575049007 23.324431059465294 9 21.739068670625336 19.809926982413273 19.910787851237323 38.54021649572407 10-11 21.561496718470323 27.1642469528653 26.713214194391565 24.561042134272807 12-13 21.09483762820695 24.98792510725346 25.65630593516493 28.26093132937466 14-15 20.30996959968179 26.262181435917835 22.710032105008953 30.717816859391426 16-17 21.704974855811574 27.934198937409437 24.503508821774584 25.857317385004404 18-19 20.230417365116345 27.368809841747876 24.830241213739807 27.570531579395976 20-21 19.174219393698326 27.897974259169818 25.26493735261528 27.662868994516575 22-23 22.05514674546126 27.324771997613436 24.58377134414865 26.036309912776655 24-25 20.677898684546978 29.093388641077368 24.568145012359007 25.660567662016646 26-27 19.34610904338438 29.639599965906182 25.764269682075174 25.250021308634256 28-29 20.895246753984715 27.609597408870073 27.379464158877177 24.115691678268032 30-31 20.60118760121601 27.80350598062335 27.034264283887833 24.561042134272807 32-33 18.760121601272836 29.892462425774923 26.439753388072845 24.907662584879393 34-35 20.184248657556044 28.762394522260422 26.23519049919027 24.818166320993264 36-37 20.600477313407392 27.41071682245646 26.60667102309856 25.38213484103759 38-39 20.111799301076797 27.504474813194307 27.625934028468336 24.75779185726056 40-41 19.557774810353155 27.102451913515356 27.326902861039294 26.012870415092195 42-43 18.625166917635024 26.709662755348468 28.05423757706623 26.61093274995028 44-45 18.41776287751797 26.920618234508627 28.552149330908883 26.109469557064525 46-47 18.866664772565844 27.64724266272694 28.010199732931785 25.475892831775436 48-49 18.327556325823224 28.531550984458903 27.177742421229084 25.963150268488793 50-51 18.63582123476433 27.83333806858539 26.698298150410547 26.832542546239736 52-53 17.819700542659884 27.681336477540704 27.326192573230674 27.17277040656874 54-55 17.7600363667358 28.11603261641617 26.74233599454499 27.381595022303035 56-57 18.685541381367727 27.85180555160951 26.316873597181576 27.14577946984118 58-59 18.68980310821945 27.334005739125494 26.329658777736736 27.646532374918316 60-61 18.15708725175441 27.026451117993012 26.422706480665966 28.393755149586614 62-63 18.487371082762735 27.093218172003297 26.22311560644373 28.19629513879024 64-65 19.310594652953377 27.58615791118561 24.900559706793192 28.202687729067815 66-67 18.619484615166066 28.835554166548285 24.58519191976589 27.95976929851976 68-69 18.481688780293776 28.847629059294828 24.76489473534676 27.905787425064638 70-71 18.902889450805464 28.75671221979146 24.795437111117423 27.544961218285653 72-73 19.091826007898398 28.510952638008924 24.000625053271584 28.39659630082109 74-75 18.874477938460664 28.85331136176379 24.033298292468107 28.238912407307442 76 19.11242435434838 28.73398300991562 24.22507600079552 27.928516634940475 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.5 11 3.0 12 4.0 13 3.5 14 4.0 15 4.0 16 7.5 17 12.0 18 18.0 19 27.0 20 29.0 21 28.0 22 42.0 23 61.5 24 84.5 25 102.0 26 137.5 27 224.0 28 323.5 29 372.0 30 440.5 31 606.0 32 833.0 33 963.0 34 1114.0 35 1509.5 36 1904.0 37 2054.0 38 2344.5 39 2968.0 40 3575.0 41 4111.5 42 4374.0 43 4595.5 44 5024.5 45 5247.5 46 5263.0 47 5256.0 48 5005.5 49 4523.5 50 4285.0 51 4138.0 52 3498.5 53 2791.0 54 2576.0 55 2436.5 56 2043.5 57 1564.5 58 1339.0 59 1117.5 60 783.5 61 562.5 62 454.0 63 385.5 64 276.5 65 202.5 66 145.0 67 121.0 68 106.0 69 78.0 70 57.0 71 49.0 72 33.5 73 23.0 74 20.5 75 13.0 76 15.5 77 12.5 78 11.5 79 16.0 80 12.5 81 5.5 82 3.0 83 4.0 84 2.5 85 3.0 86 4.0 87 3.0 88 4.0 89 4.0 90 3.0 91 2.0 92 1.0 93 2.0 94 3.0 95 2.5 96 2.0 97 2.0 98 3.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6492030570787283 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 70394.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.87777367389266 #Duplication Level Percentage of deduplicated Percentage of total 1 82.11929098201578 54.09836065573771 2 8.004485271919611 10.546353382390544 3 3.603312200802174 7.12134556922465 4 1.9278043731401215 5.079978407250618 5 1.1730711174364945 3.8639656788930874 6 0.8258938198128261 3.2644827684177624 7 0.5628153706818475 2.595391652697673 8 0.4636218570750852 2.443390061652982 9 0.3277698710484323 1.9433474443844645 >10 0.9919351360676241 9.043384379350512 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.008523453703440634 0.0 2 0.0 0.0 0.0 0.008523453703440634 0.0 3 0.0 0.0 0.0 0.008523453703440634 0.0 4 0.0 0.0 0.0 0.014205756172401057 0.0 5 0.0 0.0 0.0 0.01562633178964116 0.0 6 0.0 0.0 0.0 0.01704690740688127 0.0 7 0.0 0.0 0.0 0.018467483024121375 0.0 8 0.0 0.0 0.0 0.018467483024121375 0.0 9 0.0 0.0 0.0 0.019888058641361478 0.0 10 0.0 0.0 0.0 0.019888058641361478 0.0 11 0.0 0.0 0.0 0.019888058641361478 0.0 12 0.0 0.0 0.0 0.019888058641361478 0.0 13 0.0 0.0 0.0 0.019888058641361478 0.0 14 0.0 0.0 0.0 0.021308634258601584 0.0 15 0.0 0.0 0.0 0.024149785493081798 0.0 16 0.0 0.0 0.0 0.026990936727562007 0.0 17 0.0 0.0 0.0 0.028411512344802114 0.0 18 0.0 0.0 0.0 0.03125266357928232 0.0 19 0.0 0.0 0.0 0.03409381481376254 0.0 20 0.0 0.0 0.0 0.03409381481376254 0.0 21 0.0 0.0 0.0 0.03693496604824275 0.0 22 0.0 0.0 0.0 0.04261726851720317 0.0 23 0.0 0.0 0.0 0.04545841975168338 0.0 24 0.0 0.0 0.0 0.04687899536892349 0.0 25 0.0 0.0 0.0 0.0511407222206438 0.0 26 0.0 0.0 0.0 0.05256129783788391 0.0 27 0.0 0.0 0.0 0.07244935647924539 0.0 28 0.0 0.0 0.0 0.08665511265164645 0.0 29 0.0 0.0 0.0 0.09375799073784698 0.0 30 0.0 0.0 0.0 0.10938432252748814 0.0 31 0.0 0.0 0.0 0.14063698610677047 0.0 32 0.0 0.0 0.0 0.1775719521550132 0.0 33 0.0 0.0 0.0 0.21450691820325596 0.0 34 0.0 0.0 0.0 0.2500213086342586 0.0 35 0.0 0.0 0.0 0.3011620308549024 0.0 36 0.0 0.0 0.0 0.3977611728272296 0.0 37 0.0 0.0 0.0 0.5682302468960423 0.0 38 0.0 0.0 0.0 0.7742137113958576 0.0 39 0.0 0.0 0.0 1.0398613518197575 0.0 40 0.0 0.0 0.0 1.3495468363781005 0.0 41 0.0 0.0 0.0 1.681961530812285 0.0 42 0.0 0.0 0.0 2.079722703639515 0.0 43 0.0 0.0 0.0 2.4789044520839845 0.0 44 0.0 0.0 0.0 2.8198426002216097 0.0 45 0.0 0.0 0.0 3.2516975878626018 0.0 46 0.0 0.0 0.0 3.6863937267380744 0.0 47 0.0 0.0 0.0 4.045799357899821 0.0 48 0.0 0.0 0.0 4.4137284427650085 0.0 49 0.0 0.0 0.0 4.8015455862715575 0.0 50 0.0 0.0 0.0 5.162371793050544 0.0 51 0.0 0.0 0.0 5.531721453532971 0.0 52 0.0 0.0 0.0 5.982043924198085 0.0 53 0.0 0.0 0.0 6.398272580049436 0.0 54 0.0 0.0 0.0 6.781827996704265 0.0 55 0.0 0.0 0.0 7.181009745148734 0.0 56 0.0 0.0 0.0 7.52052731766912 0.0 57 0.0 0.0 0.0 7.902662158706708 0.0 58 0.0 0.0 0.0 8.250703184930535 0.0 59 0.0 0.0 0.0 8.637099752819843 0.0 60 0.0 0.0 0.0 8.99508480836435 0.0 61 0.0 0.0 0.0 9.354490439526096 0.0 62 0.0 0.0 0.0 9.702531465749923 0.0 63 0.0 0.0 0.0 10.135807029008154 0.0 64 0.0 0.0 0.0 10.537829928687104 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTAACC 15 0.002217012 69.99361 19 GTAACCT 15 0.002217012 69.99361 20 GCTATTC 20 0.0069275773 52.495205 50 GGGTAAC 20 0.0069275773 52.495205 18 TGCTCGC 20 0.0069275773 52.495205 26 CTCTGGG 20 0.0069275773 52.495205 25 CCTTGGG 20 0.0069275773 52.495205 14 GCCGTAT 20 0.0069275773 52.495205 54 CCTCTGG 20 0.0069275773 52.495205 24 CTTCCGA 35 2.0684818E-5 49.99543 18 ACGCTTC 35 2.0684818E-5 49.99543 15 TCGCCCG 30 5.844271E-4 46.662403 10 GCGTGAA 30 5.844271E-4 46.662403 59 GGCCTCG 30 5.844271E-4 46.662403 6 AGCGGAT 40 4.553207E-5 43.746006 69 GATCTCG 40 4.553207E-5 43.746006 41 TTGGCGT 35 0.0012483124 39.99634 56 GGCAGAT 35 0.0012483124 39.99634 30 GGAAGAG 35 0.0012483124 39.99634 24 ATTGGCG 35 0.0012483124 39.99634 55 >>END_MODULE