FastQCFastQC Report
Wed 25 May 2016
SRR1780358_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780358_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70394
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT28864.099781231354945No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT28284.017387845555019Illumina PCR Primer Index 2 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC10211.450407705202148No Hit
CTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC4570.6492030570787283No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG3180.45174304628235357No Hit
TATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCT2620.3721908117169077No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC1390.19746001079637468No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG1310.18609540585845386No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATATCGTATGCCGT1300.18467483024121376No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC1190.16904849845157258No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTACCTCGTATGCCGT1100.15626331789641162No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC1050.1491604398102111No Hit
CTTAACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC920.13069295678608972No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTAACTCGTATGCCGT830.11790777623092877No Hit
CTTATAACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG770.10938432252748814No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTAACTCGTATGCCGTCTT730.10370202005852772No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATCG150.002216208770.048
CTGTCGC150.002216208770.055
GTACATG207.877075E-570.01
CGAGTTC150.002216208770.060
TAAACAG150.002216208770.037
TACATGC207.877075E-570.02
TACTGAC150.002216208770.034
GAGTTCC150.002216208770.061
GCTGTCG150.002216208770.054
AGTGTTC150.002216208770.08
GACTATC150.002216208770.038
GTAAACA150.002216208770.036
TGCCGAG150.002216208770.057
CTATACA750.060.666671
AGACTTC252.3755868E-455.99999611
GCGAACT252.3755868E-455.99999628
AAAGCGA252.3755868E-455.99999625
CAAAGCG252.3755868E-455.99999624
CTTTACA1400.055.01
TTTGTAC452.6551788E-854.44444332
Produced by FastQC (version 0.11.5)