##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780358_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 70394 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.89629797994147 34.0 34.0 34.0 31.0 34.0 2 33.365258402704775 34.0 34.0 34.0 33.0 34.0 3 33.485254425093046 34.0 34.0 34.0 34.0 34.0 4 33.507969429212714 34.0 34.0 34.0 34.0 34.0 5 33.517245787993296 34.0 34.0 34.0 34.0 34.0 6 37.27780776770747 38.0 38.0 38.0 36.0 38.0 7 36.86589766173253 38.0 37.0 38.0 35.0 38.0 8 36.86317015654743 38.0 37.0 38.0 35.0 38.0 9 36.6559081739921 38.0 37.0 38.0 34.0 38.0 10-11 36.35637980509703 38.0 37.0 38.0 33.0 38.0 12-13 36.210721084183305 38.0 37.0 38.0 34.0 38.0 14-15 36.41106486348268 38.0 37.0 38.0 34.0 38.0 16-17 36.503260221041565 38.0 37.0 38.0 34.0 38.0 18-19 36.521060033525586 38.0 37.0 38.0 34.0 38.0 20-21 36.27471801573998 38.0 37.0 38.0 33.5 38.0 22-23 36.31531096400261 38.0 37.0 38.0 33.0 38.0 24-25 35.5540955195045 38.0 36.0 38.0 29.5 38.0 26-27 35.784768588231955 38.0 36.0 38.0 31.5 38.0 28-29 35.517117936187745 38.0 36.0 38.0 31.0 38.0 30-31 35.186571298690225 37.5 36.0 38.0 29.0 38.0 32-33 35.612211268005794 38.0 36.5 38.0 31.0 38.0 34-35 35.595057817427616 38.0 36.0 38.0 31.0 38.0 36-37 35.738457823109925 38.0 36.5 38.0 31.5 38.0 38-39 35.21285905048725 38.0 35.5 38.0 29.0 38.0 40-41 35.04829957098616 37.0 35.5 38.0 29.0 38.0 42-43 35.64720004545842 37.5 36.5 38.0 32.0 38.0 44-45 35.88713526721027 38.0 36.5 38.0 32.5 38.0 46-47 36.09340284683354 38.0 37.0 38.0 34.0 38.0 48-49 35.823003380969965 38.0 36.5 38.0 31.5 38.0 50-51 35.95719805665256 38.0 37.0 38.0 32.0 38.0 52-53 35.836889507628484 38.0 37.0 38.0 32.0 38.0 54-55 36.036515896241156 38.0 37.0 38.0 34.0 38.0 56-57 36.03959854533056 38.0 37.0 38.0 33.0 38.0 58-59 36.062562150183254 38.0 37.0 38.0 34.0 38.0 60-61 35.81481376253658 38.0 36.5 38.0 32.5 38.0 62-63 36.156057334431914 38.0 37.0 38.0 34.0 38.0 64-65 36.146724152626646 38.0 37.0 38.0 33.5 38.0 66-67 35.24163280961446 38.0 36.0 38.0 29.5 38.0 68-69 33.96493309088842 37.0 34.0 38.0 23.0 38.0 70-71 33.244779384606645 37.0 33.5 38.0 20.5 38.0 72-73 32.49148364917465 37.0 31.5 38.0 14.5 38.0 74-75 32.89668863823621 37.0 33.0 38.0 11.0 38.0 76 32.51392164104895 37.0 31.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4229323187146363 1101 2 0.5853940100209485 1101 3 0.3095478409031216 1101 4 0.3956822956768846 1101 5 0.3481784580781948 1101 6 -1.0169442480826874 1101 7 0.193932927650458 1101 8 0.5566581984443175 1101 9 0.3464883819322466 1101 10-11 0.010003043259033007 1101 12-13 0.7341453869239203 1101 14-15 0.5359356885028603 1101 16-17 -0.058764390984720194 1101 18-19 -0.17982693139555295 1101 20-21 -0.24637733409051066 1101 22-23 0.3992797332457769 1101 24-25 -1.7779523551579288 1101 26-27 0.12412331236794216 1101 28-29 0.3968224432919669 1101 30-31 0.7164603284460398 1101 32-33 -0.18536291013355566 1101 34-35 0.013652818840540704 1101 36-37 0.2515663229684222 1101 38-39 -0.17571245090433507 1101 40-41 0.9039295323685721 1101 42-43 0.8103733097505028 1101 44-45 0.12272966615282144 1101 46-47 0.32102401001233005 1101 48-49 -1.965754261345971 1101 50-51 -1.7992847016283449 1101 52-53 -2.105521665893498 1101 54-55 -0.9585843874522695 1101 56-57 -1.4138370385607857 1101 58-59 -1.886574465224733 1101 60-61 -1.444681497834452 1101 62-63 -1.1194563978593806 1101 64-65 -1.4166449319588708 1101 66-67 -2.4266862277823975 1101 68-69 -1.3899417443431972 1101 70-71 -1.8872707286229833 1101 72-73 -2.8608213783790184 1101 74-75 -1.3158325425541904 1101 76 -0.7400264193496007 1102 1 -0.8296723799574792 1102 2 -0.0748715670985618 1102 3 0.4672597918734951 1102 4 0.34791246251379704 1102 5 -0.06993526340630751 1102 6 0.33480924187305305 1102 7 0.08882567535219721 1102 8 0.32032861042933547 1102 9 0.4515956342305074 1102 10-11 0.3433704249410283 1102 12-13 0.6293956422865392 1102 14-15 -0.20039389951212172 1102 16-17 0.5109325787122501 1102 18-19 -0.21755250170539853 1102 20-21 0.5168504493619963 1102 22-23 0.6569235875188468 1102 24-25 -5.209838633673421 1102 26-27 -1.0137554755108482 1102 28-29 0.3398936043406522 1102 30-31 1.6553094942615019 1102 32-33 0.5066697762811501 1102 34-35 0.9479565301105453 1102 36-37 -2.5793155292617413 1102 38-39 -0.2630805135532981 1102 40-41 0.9482775064918272 1102 42-43 0.8426681684498618 1102 44-45 0.8733255122542829 1102 46-47 1.1620182217930548 1102 48-49 -3.079833253514856 1102 50-51 -0.6543068330549673 1102 52-53 0.32318109202275025 1102 54-55 0.5800094157482789 1102 56-57 0.9105415790762663 1102 58-59 0.3073479045403431 1102 60-61 -6.3599862305549095 1102 62-63 -2.7982613008215864 1102 64-65 0.3734469985825015 1102 66-67 1.0036508508692918 1102 68-69 -0.8133329598692498 1102 70-71 -1.1436377698214812 1102 72-73 -5.844665436942176 1102 74-75 -2.53193741146805 1102 76 -0.6430567223799031 1103 1 0.5896578262168433 1103 2 0.35476735512245483 1103 3 0.2983386617327781 1103 4 0.4283095389837399 1103 5 0.30919640453304 1103 6 0.32439143888407074 1103 7 0.8529797061147093 1103 8 0.7404769686708548 1103 9 0.2262765549243042 1103 10-11 0.503810045318474 1103 12-13 -0.8575139246383046 1103 14-15 -0.15645913350361695 1103 16-17 0.30704908356661775 1103 18-19 0.8357364696047398 1103 20-21 -0.058910432060500284 1103 22-23 0.6428513719630473 1103 24-25 -2.1884848647195625 1103 26-27 -0.00647392211278941 1103 28-29 0.3664017313178505 1103 30-31 1.3479843060865235 1103 32-33 1.4783617147561188 1103 34-35 0.5823789147511036 1103 36-37 -0.8116434446102758 1103 38-39 0.36438757851608017 1103 40-41 0.29831023257451506 1103 42-43 -0.09321598121742625 1103 44-45 1.0730091601216358 1103 46-47 1.0570493115716104 1103 48-49 -2.109723075703137 1103 50-51 0.19419867583561512 1103 52-53 0.8868878663218567 1103 54-55 0.10700843396579529 1103 56-57 0.960067050877285 1103 58-59 0.16038052153607651 1103 60-61 -3.9326926775932023 1103 62-63 -0.8165879071268094 1103 64-65 0.8481388279705229 1103 66-67 0.3606924129943039 1103 68-69 0.6029973553920129 1103 70-71 1.2109493361019332 1103 72-73 -3.2828131406620393 1103 74-75 -0.5363827043664884 1103 76 -1.876066431117767 1104 1 -0.26132488749379235 1104 2 -0.03190089020078091 1104 3 0.23859440409602684 1104 4 -0.7916052135618443 1104 5 -0.5842679572950473 1104 6 -0.043403967225330575 1104 7 0.39304600969036585 1104 8 0.5534061659239882 1104 9 0.7620094462337974 1104 10-11 -0.4040398244423642 1104 12-13 0.31995469956250844 1104 14-15 0.5473178023239953 1104 16-17 0.3694691640781045 1104 18-19 0.6081257663132362 1104 20-21 -0.7117875336470476 1104 22-23 0.4547860155813268 1104 24-25 0.9325428407031282 1104 26-27 1.165512817172079 1104 28-29 1.5854403294708277 1104 30-31 0.8041173868348039 1104 32-33 0.7153022783365159 1104 34-35 0.3028619836594686 1104 36-37 0.9998294419632501 1104 38-39 1.3525950139884912 1104 40-41 1.7708179285252612 1104 42-43 0.7020954087896811 1104 44-45 0.7314510260936942 1104 46-47 0.9434925983345792 1104 48-49 0.1962560860302247 1104 50-51 0.43138787782472576 1104 52-53 0.45634086182269584 1104 54-55 -0.06560582130297377 1104 56-57 -0.14007502821340267 1104 58-59 -1.214068921987483 1104 60-61 0.3057989160605956 1104 62-63 0.3095901653335247 1104 64-65 0.5600734715888009 1104 66-67 0.4708747478273665 1104 68-69 1.3136059274971572 1104 70-71 1.5745178892631913 1104 72-73 1.060390742833107 1104 74-75 0.6784764005352386 1104 76 0.21547986298595134 1105 1 0.6887331845154989 1105 2 0.407471932098872 1105 3 0.23162576298103943 1105 4 0.1251195251141155 1105 5 -0.12108561118586891 1105 6 0.16054492940649112 1105 7 0.7346255683430982 1105 8 -0.6814370396509233 1105 9 0.6343671698110356 1105 10-11 0.4582714415274367 1105 12-13 -0.19572474294620434 1105 14-15 0.41450711707428667 1105 16-17 0.30855162633130107 1105 18-19 0.5935496919810674 1105 20-21 0.85470491699175 1105 22-23 0.1914875254535673 1105 24-25 0.7841688569632836 1105 26-27 0.5505302387748685 1105 28-29 0.6682510147205392 1105 30-31 1.314512276497986 1105 32-33 0.9864985617279203 1105 34-35 -0.5380787828910556 1105 36-37 0.7911767125788103 1105 38-39 0.15091092571903886 1105 40-41 0.39375637219541204 1105 42-43 0.26080621018340366 1105 44-45 0.6757599691831189 1105 46-47 0.502842191830517 1105 48-49 -0.023546469138189252 1105 50-51 0.7013646490210093 1105 52-53 0.5514047410329113 1105 54-55 0.7762640973962149 1105 56-57 0.505859931136186 1105 58-59 0.35823072958046254 1105 60-61 0.7884353852824333 1105 62-63 -0.6021403805433607 1105 64-65 0.5634416481277142 1105 66-67 0.4947423436461804 1105 68-69 0.20204959764814134 1105 70-71 0.8270149856627285 1105 72-73 0.7484859809283364 1105 74-75 -0.7991658758875246 1105 76 0.15694327762009408 1106 1 -0.24221919643687784 1106 2 0.23286875749570157 1106 3 0.12924481060009185 1106 4 0.11083381082840305 1106 5 0.13605724595698376 1106 6 0.24467191353348028 1106 7 -0.8717236380061095 1106 8 0.45110264288876323 1106 9 -0.6100772746334115 1106 10-11 0.5241444574004532 1106 12-13 1.4503070030855412 1106 14-15 -0.2279532003860325 1106 16-17 -0.0585118657321928 1106 18-19 0.14593064436201786 1106 20-21 0.5753398376266645 1106 22-23 0.5823605413265867 1106 24-25 -0.12972003192560777 1106 26-27 0.9362445244891546 1106 28-29 0.7912668877364126 1106 30-31 0.44070275268845904 1106 32-33 0.3555461807755407 1106 34-35 1.1111275663152895 1106 36-37 1.5169703633724652 1106 38-39 0.07908552889363563 1106 40-41 1.3370103404493818 1106 42-43 0.9790601784373791 1106 44-45 0.9618710802942303 1106 46-47 0.9278421918305142 1106 48-49 1.18677099117928 1106 50-51 0.07874560140196252 1106 52-53 0.2518015664297337 1106 54-55 0.21793076406288492 1106 56-57 0.19752659780285597 1106 58-59 1.0475164438661793 1106 60-61 0.9626417344887841 1106 62-63 0.6863516829486969 1106 64-65 -0.7817964471103878 1106 66-67 0.3205359944398225 1106 68-69 0.9393511849497216 1106 70-71 -0.309492950845204 1106 72-73 0.11039074283310413 1106 74-75 -1.1908325425541904 1106 76 0.18472105539787265 1107 1 0.5878431088590688 1107 2 0.046991291244403044 1107 3 0.1627339071732976 1107 4 0.30989923138914577 1107 5 0.20770833629966745 1107 6 0.3860010931804112 1107 7 0.6814435478713605 1107 8 0.38438405514213514 1107 9 -0.23448018221388622 1107 10-11 0.5008838135790654 1107 12-13 0.5090816655985222 1107 14-15 0.62534378819133 1107 16-17 0.8543905226374804 1107 18-19 0.6921923266050101 1107 20-21 0.7715495987896972 1107 22-23 0.02335223395378705 1107 24-25 0.6667233740972378 1107 26-27 0.011945459068591902 1107 28-29 0.7835825679025561 1107 30-31 0.7425719115669693 1107 32-33 0.928910666756849 1107 34-35 0.5562469847991949 1107 36-37 0.8924895741720462 1107 38-39 0.4874707833069323 1107 40-41 0.7543260206155296 1107 42-43 0.5361733663916866 1107 44-45 0.5643892526722141 1107 46-47 1.0534652447900115 1107 48-49 0.7720254044710728 1107 50-51 1.0377019876947955 1107 52-53 0.6326686484650423 1107 54-55 0.9041457173339111 1107 56-57 1.1060156943760617 1107 58-59 0.7963482195671148 1107 60-61 1.1064371654337464 1107 62-63 0.7631689207472476 1107 64-65 1.0206957647276198 1107 66-67 -0.320144171707625 1107 68-69 0.9142213822498277 1107 70-71 -0.2318190152273445 1107 72-73 -0.04848776183979453 1107 74-75 -1.1549291157629114 1107 76 -0.13371092798738005 1108 1 -0.32757273179041846 1108 2 0.10155562618256653 1108 3 -0.060149128793852924 1108 4 0.3694196694142633 1108 5 0.08050169040143373 1108 6 -0.23714626828470386 1108 7 0.20504403876157085 1108 8 -0.6170791752930569 1108 9 -0.14947121402735064 1108 10-11 0.17363940689540414 1108 12-13 1.044246397024935 1108 14-15 0.17280437537154114 1108 16-17 -0.438562370782698 1108 18-19 0.16764781607919588 1108 20-21 0.9450368073236319 1108 22-23 0.054835288801328375 1108 24-25 0.8013405741350041 1108 26-27 -0.3369877987431664 1108 28-29 -0.9506523041827819 1108 30-31 0.1967633587490738 1108 32-33 0.8070613322906937 1108 34-35 0.19648110166882304 1108 36-37 1.2758087472108457 1108 38-39 1.1960047208128302 1108 40-41 0.8660507444897831 1108 42-43 -0.18255598317878707 1108 44-45 0.7186892621124201 1108 46-47 0.7679937069820255 1108 48-49 1.0963669507752414 1108 50-51 0.7093011569575154 1108 52-53 -0.025218635590462668 1108 54-55 -0.02727125613913728 1108 56-57 -0.25828148300522713 1108 58-59 0.0588800802298195 1108 60-61 0.7674397142867662 1108 62-63 0.9633718849689075 1108 64-65 0.8838601185461812 1108 66-67 0.7758390247428579 1108 68-69 1.2060178516163873 1108 70-71 -1.0453515367037909 1108 72-73 -0.1976900652476985 1108 74-75 -0.9612870880087314 1108 76 -0.052147631470809586 1109 1 -0.8153451932470901 1109 2 0.30118348370515946 1109 3 0.3545239174581454 1109 4 0.34815438499108353 1109 5 -0.18132712405896 1109 6 -0.5359235144303724 1109 7 -0.39649769435911253 1109 8 0.26224033560539795 1109 9 0.5062969943724411 1109 10-11 0.3379137290644607 1109 12-13 0.5532841429047863 1109 14-15 0.7152020361902629 1109 16-17 0.709497172013684 1109 18-19 0.5868397352711128 1109 20-21 0.9651059194980149 1109 22-23 0.5263929708853254 1109 24-25 -0.03744198833177137 1109 26-27 0.011364141714032883 1109 28-29 -1.1465587369313255 1109 30-31 0.29046351823870253 1109 32-33 0.5873643625937248 1109 34-35 0.20392395121693596 1109 36-37 1.2215822719317373 1109 38-39 0.8273178521259652 1109 40-41 0.44140959616442643 1109 42-43 0.901441890292773 1109 44-45 0.5780725688641439 1109 46-47 0.824213802675807 1109 48-49 0.3989985297226042 1109 50-51 -0.09705184676390388 1109 52-53 -0.4777704697212499 1109 54-55 -0.44247593450170797 1109 56-57 0.03704015442167474 1109 58-59 -0.43633523077496505 1109 60-61 1.0305976090236015 1109 62-63 0.2929837935281796 1109 64-65 0.8728287522516567 1109 66-67 0.3187683176721521 1109 68-69 0.026326198240532506 1109 70-71 0.10523325861784372 1109 72-73 0.09484050359865392 1109 74-75 0.2619983984346419 1109 76 0.05072318192631542 1110 1 -0.5190952871873193 1110 2 -0.46201198712326885 1110 3 -0.03524035519397728 1110 4 0.16004847051426196 1110 5 0.3856209457824633 1110 6 0.2878947174892659 1110 7 0.5844136511154758 1110 8 0.8282114270656038 1110 9 1.0764265066347676 1110 10-11 -1.0518881196792478 1110 12-13 0.7326223026783296 1110 14-15 0.9514679747157686 1110 16-17 0.5659064007017847 1110 18-19 -0.24228925092593556 1110 20-21 0.9117854455382428 1110 22-23 -0.7932793655960495 1110 24-25 -1.0436670941710915 1110 26-27 0.8828413831278965 1110 28-29 0.38233727749789637 1110 30-31 -0.13573703788745206 1110 32-33 0.5181116258017155 1110 34-35 0.8074335581710272 1110 36-37 1.4673048601729306 1110 38-39 0.6719156626923635 1110 40-41 -0.6888040865430582 1110 42-43 -0.06660590649572384 1110 44-45 0.6735784683105237 1110 46-47 0.5246572005565113 1110 48-49 1.0362765176481545 1110 50-51 -0.1599542240779641 1110 52-53 0.313945254620549 1110 54-55 -0.18246190609418278 1110 56-57 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0.6736241101424909 2116 38-39 -3.4012762282123035 2116 40-41 -1.2721627861656088 2116 42-43 -1.137864886162106 2116 44-45 0.00521733352420739 2116 46-47 -0.18998726553382994 2116 48-49 0.24013016430588863 2116 50-51 -0.9819133133267215 2116 52-53 0.21297727702405211 2116 54-55 -0.5128831894254873 2116 56-57 -0.3044113866932747 2116 58-59 -1.792599835203589 2116 60-61 -0.9484693766223273 2116 62-63 0.6105118896670518 2116 64-65 -2.3193933463351897 2116 66-67 -0.6394769254568189 2116 68-69 -1.5252482982544038 2116 70-71 0.4817215194390343 2116 72-73 -0.5436790246087568 2116 74-75 -0.0069178138720218385 2116 76 -0.3698009084264129 2117 1 0.5497846571276668 2117 2 0.5523934845348748 2117 3 0.33781899171762575 2117 4 -0.3677789059346068 2117 5 0.32488190877008805 2117 6 0.32938610749622654 2117 7 0.8445897852437341 2117 8 0.3385464450724456 2117 9 -1.0729546028286592 2117 10-11 -0.36962561324823184 2117 12-13 -1.2096801516993025 2117 14-15 0.14730370391711745 2117 16-17 -1.1306859183975817 2117 18-19 -1.203491320955905 2117 20-21 -0.19309304612414024 2117 22-23 -0.0812072171833691 2117 24-25 -1.5049448231908613 2117 26-27 -2.711732354123562 2117 28-29 -1.2706149362841472 2117 30-31 0.31180101858441844 2117 32-33 -2.1840491949470007 2117 34-35 0.6579162625259585 2117 36-37 -0.8572268106802028 2117 38-39 -0.053396808935517015 2117 40-41 -0.31432235062319336 2117 42-43 0.04424964536094933 2117 44-45 -0.3902323236877834 2117 46-47 -0.9152695615413577 2117 48-49 -1.1273587454938152 2117 50-51 -0.12900969724929467 2117 52-53 0.4785035574380885 2117 54-55 -0.22383224171746718 2117 56-57 0.038326212446399666 2117 58-59 -0.8809517642263032 2117 60-61 -1.0778046367379375 2117 62-63 -0.6223991198772438 2117 64-65 -1.15915675539555 2117 66-67 -1.0937382509037832 2117 68-69 0.543847010254801 2117 70-71 0.4452918918009061 2117 72-73 1.328598835318651 2117 74-75 1.4146106173687372 2117 76 1.5997178440940871 2118 1 -0.18110808532576783 2118 2 0.36620209082903443 2118 3 -0.8374218560665767 2118 4 0.03305603305062732 2118 5 0.3582794681792052 2118 6 0.24467191353348028 2118 7 0.8504985842161119 2118 8 0.48443597622209467 2118 9 -1.7878550524111887 2118 10-11 -1.0175222092662182 2118 12-13 -1.4024707746922402 2118 14-15 0.2664912440584146 2118 16-17 -3.2140674212877514 2118 18-19 -0.6235138000824278 2118 20-21 -0.2746601623733369 2118 22-23 0.7073605413265796 2118 24-25 0.22583552362994652 2118 26-27 -2.469311031066404 2118 28-29 -0.2698442233747045 2118 30-31 -0.545408358422641 2118 32-33 0.12776840299775927 2118 34-35 -0.04720576701804191 2118 36-37 -3.2524740810719805 2118 38-39 0.10130775111586132 2118 40-41 -0.7546563262172867 2118 42-43 0.359615733992932 2118 44-45 -1.4797955863724326 2118 46-47 0.33339774738606565 2118 48-49 0.46177099117927156 2118 50-51 -0.8073655097091503 2118 52-53 -1.1898651002369292 2118 54-55 -0.8404025692704451 2118 56-57 0.5141932644695189 2118 58-59 -0.6024835561338193 2118 60-61 -0.723469376622333 2118 62-63 -1.2414260948290803 2118 64-65 -0.13735200266594205 2118 66-67 0.5955359944398282 2118 68-69 -1.6884265928280513 2118 70-71 0.6099514935992403 2118 72-73 1.1270574094997698 2118 74-75 1.0091674574458125 2118 76 0.9513877220645384 2119 1 0.7077808035631179 2119 2 -0.8051429384107323 2119 3 0.013747734576696757 2119 4 -0.3605112184113679 2119 5 -0.05107725696699106 2119 6 -0.8564976771098003 2119 7 -1.4512558017488004 2119 8 -0.2550377079884356 2119 9 -0.40598370738194944 2119 10-11 -0.4425222092662153 2119 12-13 -0.6917982600723533 2119 14-15 -0.2786841945380729 2119 16-17 -0.4159680060830695 2119 18-19 -0.6736599989128393 2119 20-21 -1.9705665951218734 2119 22-23 -0.46164530662663594 2119 24-25 0.6996659329866759 2119 26-27 0.3590515420330149 2119 28-29 -0.5979143988133018 2119 30-31 0.4196501211095196 2119 32-33 0.15408419247144423 2119 34-35 0.16989949613985544 2119 36-37 -0.39165536762168784 2119 38-39 -1.65673318455665 2119 40-41 -0.852317144930737 2119 42-43 -0.13205093267373513 2119 44-45 -0.07131605420868681 2119 46-47 0.10810534972524977 2119 48-49 -0.2886676053119501 2119 50-51 0.8043303967236071 2119 52-53 -0.16091773181587854 2119 54-55 0.15374947751318047 2119 56-57 -1.1394617062907173 2119 58-59 -0.3172496380051655 2119 60-61 -0.08077931814278827 2119 62-63 -1.0398179076945695 2119 64-65 -0.8762408915548221 2119 66-67 0.5715593862526873 2119 68-69 1.1095266235462233 2119 70-71 -0.007738564880298782 2119 72-73 1.1118527311371977 2119 74-75 -0.1526746478173493 2119 76 -0.1986122779354602 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.0 21 4.0 22 5.0 23 21.0 24 53.0 25 96.0 26 224.0 27 381.0 28 663.0 29 1046.0 30 1584.0 31 2459.0 32 3594.0 33 5360.0 34 7553.0 35 9785.0 36 14332.0 37 23232.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.232945989715034 23.469329772423787 11.513765377731056 31.783958860130124 2 16.184618007216525 17.693269312725516 44.8248430263943 21.297269653663665 3 15.082251328238202 27.29493990965139 35.60814842174049 22.014660340369918 4 10.618802738869789 22.940875642810465 37.40375600193198 29.03656561638776 5 9.94118816944626 40.534704662329176 37.53586953433531 11.988237633889252 6 21.91237889592863 40.71511776571867 17.31965792539137 20.052845412961332 7 21.879705656732106 35.373753444895875 20.736142284853823 22.0103986135182 8 19.305622638293034 37.11253800039776 22.875529164417422 20.70631019689178 9 19.507344375941134 18.115180271045826 19.128050686138025 43.24942466687502 10-11 19.857516265590817 25.89496263886127 30.705741966644883 23.54177912890303 12-13 22.642554763190045 20.17288405261812 27.928516634940475 29.25604454925136 14-15 22.61130209961076 23.64477086115294 21.214165980055117 32.529761059181176 16-17 24.014120521635366 27.500923374151203 21.36758814671705 27.11736795749638 18-19 22.29593431258346 26.642895701338183 22.16524135579737 28.89592863028099 20-21 21.111174247805213 24.778390203710543 22.133278404409467 31.97715714407478 22-23 23.109924141262038 29.18501576838935 22.670255987726225 25.03480410262238 24-25 23.77759468136489 31.006903997499784 22.41100093757991 22.804500383555418 26-27 21.783816802568403 28.72545955621218 22.70150865130551 26.789214989913912 28-29 21.64602096769611 23.988550160525044 26.021393868795634 28.344035002983208 30-31 22.825098730005397 22.44296388896781 30.39250504304344 24.339432337983354 32-33 21.685086797170214 22.939455067193226 35.800636417876525 19.574821717760035 34-35 23.01758672614143 25.613688666647725 33.0475608716652 18.321163735545642 36-37 19.61175668380828 27.13157371366878 31.20294343267892 22.05372616984402 38-39 20.69920731880558 24.280478449867886 28.695627468250134 26.3246867630764 40-41 19.90155410972526 24.9964485609569 30.505440804614032 24.59655652470381 42-43 24.144103190612835 24.139841463761115 31.635508708128533 20.080546637497516 44-45 19.645140210813423 26.162741142711027 29.246100519930675 24.946018126544875 46-47 24.282609313293747 22.330028127397224 27.544961218285653 25.84240134102338 48-49 22.000454584197517 22.197914594993893 28.584112282296786 27.217518538511804 50-51 18.020001704690742 22.393954030173028 32.08938261783675 27.496661647299486 52-53 19.63164474244964 22.232008409807655 36.06060175583146 22.07574509191124 54-55 18.214620564252634 22.796687217660597 32.57735034235873 26.411341875728045 56-57 23.10069039974998 22.109838906725006 29.533767082421797 25.25570361110322 58-59 18.721055771798735 28.191323124129898 31.790351450407705 21.297269653663665 60-61 20.51240162513851 34.62653067022758 24.83308236497429 20.02798533965963 62-63 16.343012188538797 39.75409836065574 23.180242634315423 20.72264681649004 64-65 16.453106798874902 40.89268971787368 22.157428189902546 20.496775293348865 66-67 16.568883711679973 40.095746796601986 21.948603574168253 21.38676591754979 68-69 16.24428218314061 38.451430519646564 22.802369520129556 22.501917777083275 70-71 16.730119044236723 36.057760604596986 23.151831121970623 24.060289229195668 72-73 17.187544392988038 33.706707958064605 23.042446799443134 26.063300849504216 74-75 17.263545188510385 33.01133619342558 23.51549847998409 26.20962013807995 76 17.328181379094808 33.0653180668807 23.39403926471006 26.212461289314433 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 1.0 15 2.5 16 3.0 17 3.0 18 4.5 19 9.0 20 12.5 21 13.0 22 26.5 23 52.0 24 60.5 25 57.0 26 105.0 27 189.5 28 273.0 29 320.0 30 373.5 31 498.0 32 742.5 33 916.0 34 1056.0 35 1364.5 36 1630.0 37 1727.0 38 2077.0 39 2982.0 40 4582.0 41 6357.5 42 7088.0 43 7148.5 44 6899.0 45 6165.0 46 5741.0 47 5348.5 48 4573.0 49 3835.0 50 3480.0 51 3258.0 52 2668.5 53 2084.5 54 1868.0 55 1746.0 56 1382.5 57 1020.0 58 899.0 59 679.0 60 401.0 61 288.5 62 234.0 63 186.0 64 118.5 65 83.5 66 56.5 67 45.0 68 29.0 69 11.5 70 6.5 71 3.0 72 2.0 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 70394.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.19944881666051 #Duplication Level Percentage of deduplicated Percentage of total 1 64.14940985228638 25.787709179759638 2 15.029330694748747 12.083416200244338 3 7.145381299031733 8.617211694178481 4 3.8377270478479044 6.170980481291019 5 2.519612693476571 5.0643520754609765 6 1.745706410347021 4.210586129499673 7 1.2545056187716448 3.5301304088416625 8 0.9399957594176268 3.022984913486945 9 0.6184182627747544 2.2374065971531665 >10 2.6821683511202203 16.241441031906128 >50 0.035338186444271676 0.9503650879336307 >100 0.03180436779984451 2.5158394181322272 >500 0.0 0.0 >1k 0.010601455933281503 9.567576782112113 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT 2886 4.099781231354945 No Hit ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT 2828 4.017387845555019 Illumina PCR Primer Index 2 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC 1021 1.450407705202148 No Hit CTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC 457 0.6492030570787283 No Hit TCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG 318 0.45174304628235357 No Hit TATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCT 262 0.3721908117169077 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC 139 0.19746001079637468 No Hit CTTAATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG 131 0.18609540585845386 No Hit CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATATCGTATGCCGT 130 0.18467483024121376 No Hit TCTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC 119 0.16904849845157258 No Hit CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTACCTCGTATGCCGT 110 0.15626331789641162 No Hit TCTTAATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC 105 0.1491604398102111 No Hit CTTAACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC 92 0.13069295678608972 No Hit CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTAACTCGTATGCCGT 83 0.11790777623092877 No Hit CTTATAACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG 77 0.10938432252748814 No Hit ATACACATCTCCGAGCCCACGAGACCCGTTTGTAACTCGTATGCCGTCTT 73 0.10370202005852772 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.011364604937920846 0.0 2 0.0 0.0 0.0 0.01278518055516095 0.0 3 0.0 0.0 0.0 0.01278518055516095 0.0 4 0.0 0.0 0.0 0.01278518055516095 0.0 5 0.0 0.0 0.0 0.014205756172401057 0.0 6 0.0 0.0 0.0 0.01562633178964116 0.0 7 0.0 0.0 0.0 0.01704690740688127 0.0 8 0.0 0.0 0.0 0.01704690740688127 0.0 9 0.0 0.0 0.0 0.018467483024121375 0.0 10 0.0 0.0 0.0 0.018467483024121375 0.0 11 0.0 0.0 0.0 0.018467483024121375 0.0 12 0.0 0.0 0.0 0.018467483024121375 0.0 13 0.0 0.0 0.0 0.018467483024121375 0.0 14 0.0 0.0 0.0 0.019888058641361478 0.0 15 0.0 0.0 0.0 0.021308634258601584 0.0 16 0.0 0.0 0.0 0.0255703611103219 0.0 17 0.0 0.0 0.0 0.026990936727562007 0.0 18 0.0 0.0 0.0 0.028411512344802114 0.0 19 0.0 0.0 0.0 0.02983208796204222 0.0 20 0.0 0.0 0.0 0.03125266357928232 0.0 21 0.0 0.0 0.0 0.03693496604824275 0.0 22 0.0 0.0 0.0 0.04261726851720317 0.0 23 0.0 0.0 0.0 0.04545841975168338 0.0 24 0.0 0.0 0.0 0.04545841975168338 0.0 25 0.0 0.0 0.0 0.0497201466034037 0.0 26 0.0 0.0 0.0 0.0511407222206438 0.0 27 0.0 0.0 0.0 0.06960820524476517 0.0 28 0.0 0.0 0.0 0.08097281018268603 0.0 29 0.0 0.0 0.0 0.08807568826888655 0.0 30 0.0 0.0 0.0 0.10512259567576782 0.0 31 0.0 0.0 0.0 0.13353410802056995 0.0 32 0.0 0.0 0.0 0.1775719521550132 0.0 33 0.0 0.0 0.0 0.21166576696877576 0.0 34 0.0 0.0 0.0 0.2528624598687388 0.0 35 0.0 0.0 0.0 0.3054237577066227 0.0 36 0.0 0.0 0.0 0.4062846265306702 0.0 37 0.0 0.0 0.0 0.5753331249822428 0.0 38 0.0 0.0 0.0 0.7955223456544592 0.0 39 0.0 0.0 0.0 1.089581498423161 0.0 40 0.0 0.0 0.0 1.397846407364264 0.0 41 0.0 0.0 0.0 1.758672614143251 0.0 42 0.0 0.0 0.0 2.1706395431428813 0.0 43 0.0 0.0 0.0 2.6124385601045543 0.0 44 0.0 0.0 0.0 2.971844191266301 0.0 45 0.0 0.0 0.0 3.4221666619314144 0.0 46 0.0 0.0 0.0 3.8753302838310084 0.0 47 0.0 0.0 0.0 4.273091456658238 0.0 48 0.0 0.0 0.0 4.655226297695826 0.0 49 0.0 0.0 0.0 5.079978407250618 0.0 50 0.0 0.0 0.0 5.466374975139927 0.0 51 0.0 0.0 0.0 5.855612694263716 0.0 52 0.0 0.0 0.0 6.32014092110123 0.0 53 0.0 0.0 0.0 6.734949001335341 0.0 54 0.0 0.0 0.0 7.154018808421172 0.0 55 0.0 0.0 0.0 7.590135522913885 0.0 56 0.0 0.0 0.0 7.946700002841151 0.0 57 0.0 0.0 0.0 8.367190385544223 0.0 58 0.0 0.0 0.0 8.73369889479217 0.0 59 0.0 0.0 0.0 9.14140409694008 0.0 60 0.0 0.0 0.0 9.53348296729835 0.0 61 0.0 0.0 0.0 9.902832627780777 0.0 62 0.0 0.0 0.0 10.275023439497684 0.0 63 0.0 0.0 0.0 10.738131090717959 0.0 64 0.0 0.0 0.0 11.171406653976192 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAAATCG 15 0.0022162087 70.0 48 CTGTCGC 15 0.0022162087 70.0 55 GTACATG 20 7.877075E-5 70.0 1 CGAGTTC 15 0.0022162087 70.0 60 TAAACAG 15 0.0022162087 70.0 37 TACATGC 20 7.877075E-5 70.0 2 TACTGAC 15 0.0022162087 70.0 34 GAGTTCC 15 0.0022162087 70.0 61 GCTGTCG 15 0.0022162087 70.0 54 AGTGTTC 15 0.0022162087 70.0 8 GACTATC 15 0.0022162087 70.0 38 GTAAACA 15 0.0022162087 70.0 36 TGCCGAG 15 0.0022162087 70.0 57 CTATACA 75 0.0 60.66667 1 AGACTTC 25 2.3755868E-4 55.999996 11 GCGAACT 25 2.3755868E-4 55.999996 28 AAAGCGA 25 2.3755868E-4 55.999996 25 CAAAGCG 25 2.3755868E-4 55.999996 24 CTTTACA 140 0.0 55.0 1 TTTGTAC 45 2.6551788E-8 54.444443 32 >>END_MODULE