FastQCFastQC Report
Wed 25 May 2016
SRR1780357_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780357_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences626106
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCACGA250.00236303835.1662063
TCCGTAT602.9251532E-729.3520513
GTACACG300.00576769529.307517
GATTACG300.00576994729.3051723
CGGTGGT1700.028.45234343
CATCGTT703.5988705E-828.2743632
CGTGTAT407.0087035E-427.51314735
CGGAATC655.8659316E-727.1072120
ACTCGCA655.8700243E-727.10504221
GTAAACA905.0931703E-1026.90174136
CTCTAAT1051.10958354E-1025.1548751
CGTAGCG801.295648E-724.7737215
GTATCAA801.301687E-724.76183334
GGCTAAA901.5019396E-824.456131
CTTTATA450.001404650424.4229242
TCGGTGG2300.023.89962642
GCGGAAT752.044515E-623.48915519
TCGTTGT852.3380744E-723.30525234
CTATACA1900.023.1689641
TTGGTAA1053.07773E-923.0383733
Produced by FastQC (version 0.11.5)