FastQCFastQC Report
Wed 25 May 2016
SRR1780357_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780357_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences626106
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT332375.308526032333183No Hit
ATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCTT272804.357089694077361RNA PCR Primer, Index 20 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC92601.4789827920511862No Hit
CTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC43040.687423535311912No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG38270.6112383526112192No Hit
TATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCT24740.3951407589130275No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC18950.3026644050687903No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC14640.2338262211191076No Hit
CTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG13210.210986638045315No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC10260.16387001562035822No Hit
CTTATAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG8790.140391563089956No Hit
CTTAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC7470.11930887102183975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT29300.042.72153544
GCCGTCT32650.038.33815843
CTTATAA1000.037.434111
TTTACAC11000.036.198292
GTAGCCG250.002352474335.19833825
CTTTACA11500.034.6576651
TTACACA11950.033.872883
CTATACA6200.033.0300941
CGTTGAG353.2169948E-431.42708830
TCTTTAT2050.031.1503331
TTATACA50450.030.3494112
CTTATAC52900.029.5959661
GACCTTT300.005717395329.3600831
GTATCGA300.005739767629.33663411
CGAGAAT300.005739767629.33663414
GAATTCG300.005744250529.33194724
TCGAACA300.005744250529.33194728
CTTTATA2250.028.3542182
TTGCGAT554.9578575E-627.99867816
GCGCTAT554.9578575E-627.99867819
Produced by FastQC (version 0.11.5)