FastQCFastQC Report
Wed 25 May 2016
SRR1780356_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780356_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50532
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGTT207.859966E-569.9920844
TGCCCTT150.00221356769.9920840
CCCTAGT200.00670244352.9131741
CAGGTGC200.00691689952.4940657
GATCCGC200.00691689952.4940628
AGGACAG200.00691689952.4940634
AAATCCC400.002399002234.9960440
TGCACGA802.4096407E-630.62153470
ATCCACA750.001810179923.3306944
ATGCACG1051.9927958E-523.33069269
GATGCAC1300.002252013716.15201868
ACGATGC1707.992798E-414.41013466
CGATGCA1600.00894615913.12351567
CGACGAT2559.469664E-512.35154270
GACGATG2100.00392047811.66534665
CCGACGA3053.8930477E-511.47411269
GCCGACG3803.2044518E-510.13043370
TGCCGAC3904.2644926E-59.87067869
CGCTGCC5006.8282716E-69.0989764
GCTGCCG4552.2913497E-48.46058165
Produced by FastQC (version 0.11.5)