FastQCFastQC Report
Wed 25 May 2016
SRR1780356_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780356_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50532
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT25735.091823003245468No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTT24884.923612760231141TruSeq Adapter, Index 9 (95% over 23bp)
CTTTACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC9211.8226074566611257RNA PCR Primer, Index 9 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC4250.8410512150716377TruSeq Adapter, Index 9 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG2460.4868202327238186No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGT2140.4234940235890129No Hit
TATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCT2080.4116203593762368RNA PCR Primer, Index 9 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC1380.27309427689384946No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGTCTT1220.24143117232644662No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC1120.22164173197181986No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG980.19393651547534235No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGTCTT940.18602073933349164No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG900.17810496319164093No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGT890.17612601915617826No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGACCTCGTATGCCGT850.16821024301432755No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC710.14050502651785007No Hit
CTTAACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC700.1385260824823874TruSeq Adapter, Index 9 (95% over 21bp)
CTTATCACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC600.11873664212776064TruSeq Adapter, Index 9 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTGGATCTGACCTCGTATGCCGTCTT570.1127998100213726No Hit
TTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC530.1048840338795219TruSeq Adapter, Index 9 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATGG150.002212573870.000011
TACCAGC150.002212573870.0000117
AAAAGCA150.002212573870.0000170
GTTAGGC150.002212573870.000013
CCGTATG150.002212573870.0000140
CGAGGTG150.002212573870.0000126
AAATTCG150.002212573870.0000151
GGCATGT150.002212573870.0000129
AGCTCCG150.002212573870.0000121
TGAATTG150.002212573870.0000143
ATTGCAA150.002212573870.0000146
TTACCAG150.002212573870.0000116
TGTAAGG150.002212573870.0000112
CTTTACA1650.067.8787841
TTTACAC1800.058.3333362
CTATACA700.055.01
CTTTATA452.634988E-854.4444472
GTGTTAG200.00691380452.527
TGGAGCT200.00691380452.525
CCTAGTA200.00691380452.52
Produced by FastQC (version 0.11.5)