##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780356_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 50532 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.85617034750257 34.0 34.0 34.0 31.0 34.0 2 33.338439008944825 34.0 34.0 34.0 33.0 34.0 3 33.48082403229637 34.0 34.0 34.0 34.0 34.0 4 33.501088419219506 34.0 34.0 34.0 34.0 34.0 5 33.51145808596533 34.0 34.0 34.0 34.0 34.0 6 37.27299532969208 38.0 37.0 38.0 36.0 38.0 7 36.89333491648856 38.0 37.0 38.0 35.0 38.0 8 36.92335549750653 38.0 37.0 38.0 35.0 38.0 9 36.64468059843268 38.0 37.0 38.0 34.0 38.0 10-11 36.36192907464577 38.0 37.0 38.0 33.5 38.0 12-13 36.21788173830444 38.0 37.0 38.0 34.0 38.0 14-15 36.400053431488956 38.0 37.0 38.0 34.0 38.0 16-17 36.478142563128316 38.0 37.0 38.0 34.0 38.0 18-19 36.518354705928914 38.0 37.0 38.0 34.0 38.0 20-21 36.25786630254096 38.0 37.0 38.0 33.5 38.0 22-23 36.27661679727697 38.0 37.0 38.0 33.0 38.0 24-25 35.462736483812236 38.0 35.5 38.0 29.0 38.0 26-27 35.689058418427926 38.0 36.0 38.0 31.0 38.0 28-29 35.53299889179134 38.0 36.0 38.0 31.0 38.0 30-31 35.12799810021372 37.5 35.5 38.0 29.0 38.0 32-33 35.66528140584184 38.0 36.0 38.0 31.5 38.0 34-35 35.517582917755085 38.0 36.0 38.0 31.0 38.0 36-37 35.51590081532494 38.0 36.0 38.0 31.0 38.0 38-39 34.77787342673949 37.5 35.0 38.0 26.5 38.0 40-41 34.87494063167894 37.0 35.0 38.0 29.0 38.0 42-43 35.59181310852529 37.5 36.0 38.0 32.0 38.0 44-45 35.842208897332384 38.0 36.5 38.0 32.0 38.0 46-47 36.06726430776537 38.0 37.0 38.0 33.5 38.0 48-49 35.84379205256076 38.0 36.5 38.0 31.5 38.0 50-51 35.89683764743133 38.0 37.0 38.0 32.0 38.0 52-53 35.77653763951555 38.0 37.0 38.0 31.5 38.0 54-55 36.01365471384469 38.0 37.0 38.0 34.0 38.0 56-57 36.01974986147392 38.0 37.0 38.0 33.0 38.0 58-59 35.99779347740046 38.0 37.0 38.0 33.5 38.0 60-61 35.77339111849917 38.0 36.5 38.0 32.5 38.0 62-63 36.15281405841843 38.0 37.0 38.0 34.0 38.0 64-65 36.12472294783504 38.0 37.0 38.0 33.5 38.0 66-67 35.16333214596691 38.0 36.0 38.0 29.0 38.0 68-69 33.653744162115096 37.0 34.0 38.0 22.0 38.0 70-71 32.735444866619176 37.0 32.0 38.0 16.0 38.0 72-73 31.814899469643002 37.0 28.5 38.0 11.0 38.0 74-75 32.08589606585926 37.0 30.0 38.0 11.0 38.0 76 31.70282197419457 37.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.10656922250079504 1101 2 0.4264421052624243 1101 3 0.12051359260012617 1101 4 0.46170354993635954 1101 5 0.3411062565092351 1101 6 -1.7468198198656069 1101 7 -0.47966087743393615 1101 8 0.21789869987968302 1101 9 0.30799247244961947 1101 10-11 -0.10477437199637052 1101 12-13 0.45495707216523584 1101 14-15 0.5955008699016702 1101 16-17 -0.42912728428017743 1101 18-19 -0.6614300199788019 1101 20-21 0.0503877737891969 1101 22-23 0.5742860342304041 1101 24-25 -2.148584791153752 1101 26-27 -0.4988627850871765 1101 28-29 0.6214309162589089 1101 30-31 0.48190906596546057 1101 32-33 -0.4111004592454819 1101 34-35 -0.4394178576334511 1101 36-37 -0.31402504208602267 1101 38-39 -0.806457969380304 1101 40-41 0.7774337005260037 1101 42-43 0.1734105440397542 1101 44-45 0.037200893494983234 1101 46-47 0.3097826767960896 1101 48-49 -2.2865957217275152 1101 50-51 -1.7318159178454096 1101 52-53 -1.876549432533345 1101 54-55 -1.7183216098091663 1101 56-57 -1.8841043340687094 1101 58-59 -1.8765472111455992 1101 60-61 -1.7839287877651557 1101 62-63 -1.0388557680287747 1101 64-65 -1.6233387502237449 1101 66-67 -2.847291119687874 1101 68-69 -2.5837846538063687 1101 70-71 -3.719306375165001 1101 72-73 -3.181094543993222 1101 74-75 -2.547789695056636 1101 76 -3.2400520267967927 1102 1 -0.6879340518971233 1102 2 0.26271257145415206 1102 3 0.31311451748451447 1102 4 0.4858880268767365 1102 5 0.3579791473978773 1102 6 0.24936815663733114 1102 7 0.08433112356593853 1102 8 0.737083801741953 1102 9 0.6708221187438355 1102 10-11 0.9370016560001346 1102 12-13 0.665993192650177 1102 14-15 -0.1994622597071256 1102 16-17 0.015660867200885775 1102 18-19 -0.3882766641482789 1102 20-21 0.5443260315069836 1102 22-23 0.6701802974475086 1102 24-25 -5.568344821149999 1102 26-27 -1.9638359134461396 1102 28-29 0.22673025384171552 1102 30-31 1.6666984672902956 1102 32-33 0.3158399232067097 1102 34-35 0.7372788052836867 1102 36-37 -3.3153373780440205 1102 38-39 -0.8726371969768536 1102 40-41 0.6468562727044826 1102 42-43 0.8013632999452653 1102 44-45 0.8963122545748519 1102 46-47 1.1377729280146909 1102 48-49 -3.14926913755054 1102 50-51 -0.29580791884528423 1102 52-53 -0.30896413069606865 1102 54-55 0.32670401198810595 1102 56-57 0.7650020276360792 1102 58-59 0.23671988046795178 1102 60-61 -6.193190130097371 1102 62-63 -2.5433864516933156 1102 64-65 -0.029038037812796347 1102 66-67 0.9649511000346607 1102 68-69 -1.2617624065872661 1102 70-71 -1.6246932018116738 1102 72-73 -6.41369046950253 1102 74-75 -3.2247050806334414 1102 76 -1.3854088571929921 1103 1 0.8031529997214264 1103 2 0.3966802005005192 1103 3 -0.04416894708241159 1103 4 0.30396545469826464 1103 5 0.3797967326997167 1103 6 0.47044208489629824 1103 7 0.5362121384390761 1103 8 0.6057955252765055 1103 9 0.16513532959248067 1103 10-11 0.02369785022585802 1103 12-13 -0.4309556262474601 1103 14-15 -0.5280110348602278 1103 16-17 0.03813462048173477 1103 18-19 0.7373795038307165 1103 20-21 0.440268726170153 1103 22-23 0.575774129468499 1103 24-25 -3.2254697117886764 1103 26-27 -0.4080889755633663 1103 28-29 0.24097456705256093 1103 30-31 1.3112741453305432 1103 32-33 1.3860225566275304 1103 34-35 0.5531416661760673 1103 36-37 -0.07444170875268696 1103 38-39 0.4137801258577909 1103 40-41 0.7496559227482322 1103 42-43 0.5241049884841971 1103 44-45 1.297121528415623 1103 46-47 1.263155692669109 1103 48-49 -1.8287584201402112 1103 50-51 -0.06366115594065036 1103 52-53 0.5505339007999908 1103 54-55 0.7290990251114735 1103 56-57 0.6878202691058988 1103 58-59 0.3868456459972549 1103 60-61 -2.9163692639556373 1103 62-63 -0.30869703787004 1103 64-65 0.9396572815222868 1103 66-67 0.6095541184073596 1103 68-69 0.4692907430190303 1103 70-71 1.390316640708015 1103 72-73 -3.380499305897981 1103 74-75 -0.762571441088383 1103 76 -2.186480598225362 1104 1 0.04667462537392453 1104 2 0.015341647182104623 1104 3 -0.06346424909583703 1104 4 -0.5675390195746672 1104 5 -0.5093251912376431 1104 6 -0.21752398520064276 1104 7 0.5231621757246856 1104 8 0.36917173705876394 1104 9 0.8303105719489281 1104 10-11 -0.9200241855683302 1104 12-13 0.11560477643710243 1104 14-15 0.6385391142821959 1104 16-17 0.022055202137217123 1104 18-19 0.7068985194906787 1104 20-21 -0.8301917510856214 1104 22-23 0.30990444266760875 1104 24-25 0.3204148892553249 1104 26-27 1.1274022623188102 1104 28-29 1.3367286684694122 1104 30-31 0.6917737724744626 1104 32-33 0.40026099808913074 1104 34-35 0.47421269525884924 1104 36-37 1.2307176872204622 1104 38-39 1.225021736596048 1104 40-41 1.3249728504141558 1104 42-43 0.08112866484512438 1104 44-45 0.5772296566781137 1104 46-47 0.7766251184707471 1104 48-49 0.1102563076748524 1104 50-51 0.05059592832482451 1104 52-53 0.35443490751140416 1104 54-55 0.20127464032399445 1104 56-57 0.2873029312982851 1104 58-59 -0.914445368171279 1104 60-61 -0.11246825724423104 1104 62-63 0.026687003889840355 1104 64-65 0.42667722932243635 1104 66-67 0.2034439394364469 1104 68-69 2.383510541676749 1104 70-71 1.8368350970838563 1104 72-73 0.745665869344375 1104 74-75 0.16852009507865517 1104 76 -0.10976546026861556 1105 1 0.7687067156890492 1105 2 0.5135860135983918 1105 3 0.48149330457322037 1105 4 -0.20196721638341586 1105 5 0.02038724042598261 1105 6 0.11342405693456215 1105 7 0.8294424548482056 1105 8 -1.0906264761951618 1105 9 0.7317284127418517 1105 10-11 0.4822381372015698 1105 12-13 0.011511261169758313 1105 14-15 0.5292231446835558 1105 16-17 0.5059878213647337 1105 18-19 0.8800395479808287 1105 20-21 0.7838577622260772 1105 22-23 0.375374019093222 1105 24-25 0.8763284854151578 1105 26-27 0.7078280590208834 1105 28-29 0.8752414912615407 1105 30-31 1.5130769415041954 1105 32-33 1.122248641984413 1105 34-35 -1.250597296296334 1105 36-37 0.6517586223731371 1105 38-39 -0.10194835096340427 1105 40-41 0.09807387712645266 1105 42-43 0.4101655109271718 1105 44-45 0.9092444128894996 1105 46-47 0.9943459060613051 1105 48-49 -0.14082151800629106 1105 50-51 0.9425244584817207 1105 52-53 0.9451944968264812 1105 54-55 1.0827138153984421 1105 56-57 0.4398208209822769 1105 58-59 0.2865191191392711 1105 60-61 0.7233244446536347 1105 62-63 -0.6837890172666548 1105 64-65 0.7303351696753424 1105 66-67 0.9101197916965447 1105 68-69 0.43886855758856314 1105 70-71 0.9389507466682758 1105 72-73 1.4381633136752292 1105 74-75 0.4409099790735027 1105 76 0.7234715724883998 1106 1 -0.7202055861371619 1106 2 0.18582161464193803 1106 3 0.11870984079637736 1106 4 0.16192000015280428 1106 5 -8.850854821034204E-4 1106 6 0.1175380444922638 1106 7 -0.9284343262073875 1106 8 0.40440663638761976 1106 9 -0.6884000239428758 1106 10-11 0.31062966840767103 1106 12-13 1.0898777070858685 1106 14-15 -0.6602711358703317 1106 16-17 0.44911946896657895 1106 18-19 0.27525829170951255 1106 20-21 0.026758625160056226 1106 22-23 0.4308877658321322 1106 24-25 -0.7346237521927179 1106 26-27 0.8359135496891597 1106 28-29 0.5777801226081181 1106 30-31 0.8781933372497335 1106 32-33 -0.5631567363017567 1106 34-35 0.7321189389033478 1106 36-37 1.106429503368524 1106 38-39 9.013379790019371E-4 1106 40-41 0.9857670338593465 1106 42-43 0.9158347864639964 1106 44-45 1.0875255688196646 1106 46-47 0.881211248224659 1106 48-49 0.9834259232941278 1106 50-51 0.008624197594706118 1106 52-53 0.6423899614060531 1106 54-55 0.10220508571752873 1106 56-57 -0.034717609681983674 1106 58-59 0.8606461510477601 1106 60-61 0.8194261905898159 1106 62-63 0.3705958914228873 1106 64-65 -1.798663425548419 1106 66-67 -0.14707466947142223 1106 68-69 1.6122831672614524 1106 70-71 0.6109605801019526 1106 72-73 0.24891988602120918 1106 74-75 0.3892593669924267 1106 76 0.6329638462190843 1107 1 0.7934307774992035 1107 2 -0.08720868838836537 1107 3 0.27416438625092354 1107 4 0.04646545469826435 1107 5 -0.009369933966951294 1107 6 0.29571986267407624 1107 7 0.5139899162168575 1107 8 -0.05226003027904369 1107 9 -0.6393091148519616 1107 10-11 0.6841145168925209 1107 12-13 0.2398777070858742 1107 14-15 0.29185007625088133 1107 16-17 0.8089679538150634 1107 18-19 0.5857128371640528 1107 20-21 0.727213170614597 1107 22-23 0.03285746280183588 1107 24-25 0.1185580659891059 1107 26-27 -0.2937834200078129 1107 28-29 0.7211134559414489 1107 30-31 0.7868297008860949 1107 32-33 1.213661445516422 1107 34-35 0.11439166617606844 1107 36-37 1.2159749579139856 1107 38-39 0.3587801258577912 1107 40-41 1.117433700526007 1107 42-43 0.6934105440397502 1107 44-45 0.8510104173045079 1107 46-47 0.871211248224661 1107 48-49 0.937213802082006 1107 50-51 0.997866621837133 1107 52-53 0.9201172341333219 1107 54-55 0.45659902511146555 1107 56-57 1.1290702691058954 1107 58-59 0.7183734237750343 1107 60-61 1.0565474027110326 1107 62-63 1.0290807399077337 1107 64-65 1.0428517259667274 1107 66-67 -0.039195881592632986 1107 68-69 1.7963740763523646 1107 70-71 0.45656664070801156 1107 72-73 1.0492229163242364 1107 74-75 -0.1260436633106039 1107 76 0.8796305128857469 1108 1 -0.9399025558341307 1108 2 0.1944351472280701 1108 3 0.4095981762052574 1108 4 0.348200614515612 1108 5 0.2990318925170641 1108 6 -0.2583440642665593 1108 7 0.16787119475566925 1108 8 -0.708607062242514 1108 9 -1.1948342290072134 1108 10-11 0.17680858081946127 1108 12-13 0.8901060175881597 1108 14-15 0.5735395739677784 1108 16-17 -0.4170594434452113 1108 18-19 0.4639776773467048 1108 20-21 0.783971161482178 1108 22-23 -0.23344390706117935 1108 24-25 0.9870968787744943 1108 26-27 -0.5538747442087271 1108 28-29 -1.337744991547133 1108 30-31 0.018656184904358497 1108 32-33 1.0233418108132284 1108 34-35 0.6928391547605486 1108 36-37 1.354924729603475 1108 38-39 1.1004239614742275 1108 40-41 0.8550592713022667 1108 42-43 -1.148507264179429 1108 44-45 0.4799601889940135 1108 46-47 0.5672843075853891 1108 48-49 0.8662092358719562 1108 50-51 0.6788711880471752 1108 52-53 0.3745921199780753 1108 54-55 -0.1404786004593106 1108 56-57 0.46336250654881894 1108 58-59 0.35768849226818844 1108 60-61 0.9248122428936796 1108 62-63 0.9919117901360508 1108 64-65 0.45901610952837757 1108 66-67 0.7150050316493761 1108 68-69 1.097287318361495 1108 70-71 -0.532200482579654 1108 72-73 0.17305853276259953 1108 74-75 -0.2009751701599214 1108 76 0.45031544439260074 1109 1 -1.0344971504287237 1109 2 0.18684536566568966 1109 3 0.3375878096743463 1109 4 0.28988887812168684 1109 5 0.26099042639340553 1109 6 -0.2377035607493454 1109 7 -0.37294702072008334 1109 8 0.49900123098221627 1109 9 0.8130332274903793 1109 10-11 0.3798352376132428 1109 12-13 0.6383011305092978 1109 14-15 1.0034266528274571 1109 16-17 0.9407247105718213 1109 18-19 0.9209380623892827 1109 20-21 1.1183843417857702 1109 22-23 0.20218178712615753 1109 24-25 -0.5695950871640463 1109 26-27 -0.08085549207988407 1109 28-29 -0.8332559134279194 1109 30-31 0.8352981693545587 1109 32-33 0.6603281121830875 1109 34-35 -0.049617342832938505 1109 36-37 1.1747136966527165 1109 38-39 1.3833747204523874 1109 40-41 0.6805868536791593 1109 42-43 1.0315186521478665 1109 44-45 0.4686680749621672 1109 46-47 0.7483283653417772 1109 48-49 0.20117776604597282 1109 50-51 -0.3588000448295361 1109 52-53 -0.29997285595676715 1109 54-55 0.04385127736372141 1109 56-57 0.22470089973652563 1109 58-59 0.11985991026151765 1109 60-61 1.1013672225308468 1109 62-63 0.4195311903581853 1109 64-65 0.9542030773180841 1109 66-67 0.42233564993889416 1109 68-69 -0.5045268245485346 1109 70-71 0.4485936677350466 1109 72-73 -0.038344651243324535 1109 74-75 -0.5734760957430396 1109 76 0.11719808045331703 1110 1 -0.7200443998057651 1110 2 -0.4877760642748896 1110 3 -0.1212966066568768 1110 4 0.22816758235783396 1110 5 0.37048822206141807 1110 6 0.1449397208301093 1110 7 0.5303019729544474 1110 8 0.9203640831961337 1110 9 1.0823930128076071 1110 10-11 -0.40205569587343604 1110 12-13 0.6324308985752367 1110 14-15 0.7770273812154187 1110 16-17 0.47740767012711416 1110 18-19 -0.6127978011338158 1110 20-21 0.7876387025294918 1110 22-23 -0.45990849464497074 1110 24-25 -1.2027894517413955 1110 26-27 1.318344239566649 1110 28-29 0.3596950162251389 1110 30-31 0.15583679308467424 1110 32-33 0.8996898143107401 1110 34-35 0.7776540775235858 1110 36-37 1.2923579366373872 1110 38-39 1.2745957286946705 1110 40-41 -1.6321407675590933 1110 42-43 0.18936799084826816 1110 44-45 0.8748402045385575 1110 46-47 0.975182879430335 1110 48-49 1.4375684120110819 1110 50-51 -0.053835505822448226 1110 52-53 0.7741597873248196 1110 54-55 -0.23765629403746402 1110 56-57 0.7297085669782319 1110 58-59 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-0.5072296119903896 2116 48-49 -0.5557969506061653 2116 50-51 -1.483800044829536 2116 52-53 -0.42628061532904127 2116 54-55 -0.6129708673616463 2116 56-57 -0.7096931717543242 2116 58-59 -1.9029168988056142 2116 60-61 -1.2843128123427334 2116 62-63 0.6276828904453708 2116 64-65 -2.501986983710694 2116 66-67 -1.8618840536356487 2116 68-69 -1.6063140956906494 2116 70-71 1.0264053503854385 2116 72-73 -0.3578738578693077 2116 74-75 0.12105311088294002 2116 76 -1.6116598096948955 2117 1 0.8474990189690175 2117 2 0.5619251698793519 2117 3 0.17138223401995134 2117 4 -0.19915134320199712 2117 5 0.027270485980551484 2117 6 -0.049844441787861626 2117 7 0.7063783676604274 2117 8 0.6032255340254125 2117 9 -0.6126424481852979 2117 10-11 -0.7992188164408134 2117 12-13 -0.8182587758537565 2117 14-15 -0.2506433620693258 2117 16-17 -0.8347328335865072 2117 18-19 -1.6599302074553677 2117 20-21 -0.44779995274498674 2117 22-23 0.1623062816994718 2117 24-25 -1.1234629313862285 2117 26-27 -2.570135126044555 2117 28-29 -1.6551595099378176 2117 30-31 -0.9238527138120673 2117 32-33 -2.2117191319114013 2117 34-35 0.316018962763998 2117 36-37 -1.0751798977290647 2117 38-39 0.048622645542828025 2117 40-41 -0.3216476643033843 2117 42-43 -0.07265244808623095 2117 44-45 -0.28872711550388885 2117 46-47 -1.8307310089931903 2117 48-49 -0.6453321296765253 2117 50-51 -0.1973827219948916 2117 52-53 0.6595398063117983 2117 54-55 -1.1693589801378792 2117 56-57 0.06815163393528678 2117 58-59 -1.5152486234690627 2117 60-61 -0.535709815136741 2117 62-63 -0.07488251468543439 2117 64-65 -1.6747073291513814 2117 66-67 -1.1885922070519541 2117 68-69 -0.32869154044553284 2117 70-71 -1.3412286348825333 2117 72-73 -0.22077708367575966 2117 74-75 0.1296256280279806 2117 76 -0.25155058947645514 2118 1 -0.49727960501445523 2118 2 -0.2934381965850861 2118 3 -1.0339230454430677 2118 4 0.04132884267640691 2118 5 -0.06521692850247263 2118 6 -0.22275008268110952 2118 7 0.7047002987305149 2118 8 0.5560459806499125 2118 9 -1.3175604809721833 2118 10-11 -1.2500384885719598 2118 12-13 -0.997553986903192 2118 14-15 0.5982981636825713 2118 16-17 -3.9902123740537903 2118 18-19 -0.8193144852403194 2118 20-21 -0.6169944796586293 2118 22-23 0.8061361513264202 2118 24-25 0.7836946780109599 2118 26-27 -2.488974676838417 2118 28-29 -0.9850614074465298 2118 30-31 -1.3285254903707369 2118 32-33 -0.015901396013639157 2118 34-35 -0.2448433065015223 2118 36-37 -2.87003597104777 2118 38-39 -0.30548216922417737 2118 40-41 -0.8345881573974907 2118 42-43 0.46029578994139797 2118 44-45 -2.9663119870670727 2118 46-47 -0.2772586971305273 2118 48-49 -0.5756277279726376 2118 50-51 -1.2604393890918288 2118 52-53 -1.9161669188721362 2118 54-55 -1.1803955104076564 2118 56-57 0.4089609794884126 2118 58-59 -1.6555610024544762 2118 60-61 -1.4076602475621982 2118 62-63 -2.142941118015763 2118 64-65 -0.438131880590646 2118 66-67 -0.2662997067292423 2118 68-69 -1.137778929112116 2118 70-71 0.30066500136375396 2118 72-73 0.9882393097668647 2118 74-75 0.4019891235746442 2118 76 0.5701223161644364 2119 1 0.6618673556702959 2119 2 -0.8185361220166882 2119 3 -0.08288576124170532 2119 4 -0.7766023919094067 2119 5 -0.6664790785097239 2119 6 -0.478734414612056 2119 7 -0.8349776354055649 2119 8 0.08361017621062672 2119 9 -0.2144123596897245 2119 10-11 -0.020457754493911295 2119 12-13 0.05300455074368671 2119 14-15 0.01128960427448078 2119 16-17 -0.41005859485750307 2119 18-19 -0.22316621888314359 2119 20-21 -1.1221083633087048 2119 22-23 -0.17192129826010927 2119 24-25 0.528617063039249 2119 26-27 0.7902578779272957 2119 28-29 -0.04980099833584006 2119 30-31 1.1315789634229674 2119 32-33 0.7289121829795491 2119 34-35 0.5762795717807947 2119 36-37 0.944087052309257 2119 38-39 -1.3218393431687545 2119 40-41 -1.1421828186480383 2119 42-43 0.5738530219158591 2119 44-45 0.5956711842661662 2119 46-47 -0.15049966622961364 2119 48-49 0.13618135370442275 2119 50-51 1.3447397781793171 2119 52-53 0.35722637867609564 2119 54-55 -0.5221915353605127 2119 56-57 -1.9652955126050244 2119 58-59 -0.3898566647205399 2119 60-61 0.32395153250454456 2119 62-63 -0.5877039208592194 2119 64-65 0.16701101800212825 2119 66-67 0.9756123779943877 2119 68-69 0.3130407430190303 2119 70-71 1.2567436318584626 2119 72-73 2.597806987120702 2119 74-75 1.4795315579283326 2119 76 0.5031703358945947 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 0.0 18 0.0 19 0.0 20 1.0 21 3.0 22 4.0 23 16.0 24 43.0 25 74.0 26 150.0 27 298.0 28 541.0 29 803.0 30 1202.0 31 1896.0 32 2853.0 33 4130.0 34 5796.0 35 7145.0 36 9711.0 37 15865.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.53383994300641 22.79743528853004 11.145412807725798 33.52331196073775 2 15.886962716694372 16.8982031188158 45.68788094672683 21.526953217763 3 15.04195361355181 26.44858703395868 36.149370695796726 22.36008865669279 4 10.866381698725561 23.199160927728965 34.948151666270874 30.986305707274596 5 10.601203197973561 39.50565977994142 36.60254887991768 13.290588142167339 6 22.36008865669279 38.54587192274202 15.817699675453179 23.276339745112008 7 21.59225837093327 36.40663342040687 18.52687406000158 23.474234148658276 8 19.201693976094354 35.24895115966121 23.09229794981398 22.45705691443046 9 18.350748040845406 19.40354626771155 18.233990342753106 44.01171534868994 10-11 19.51337766167973 24.79320034829415 30.640979973086363 25.052442016939764 12-13 22.666824982189503 18.83459985751603 27.737869073062615 30.760706087231853 14-15 23.480170980764665 22.68760389456186 19.955671653605638 33.87655347106784 16-17 24.906989630333253 27.391553866856643 20.147629225045517 27.553827277764587 18-19 22.942887675136546 26.04290350668883 20.54935486424444 30.46485395393018 20-21 21.145017018918704 23.67509696825774 20.656217842159425 34.52366817066414 22-23 23.667181192115887 29.678223699833765 20.579039024776378 26.075556083273966 24-25 24.54088498377266 31.386052402438057 20.90259637457453 23.170466239214754 26-27 25.78168289400776 28.69567798622655 24.834758173038868 20.68788094672683 28-29 24.299453811446213 25.602588458798387 27.596374574527033 22.50158315522837 30-31 28.22271036175097 23.213013535977204 26.22100846988047 22.343267632391356 32-33 22.380867569065146 26.044882450724295 30.91902161006887 20.655228370141693 34-35 19.65487216021531 25.336420486028654 32.205335233119605 22.80337212063643 36-37 22.471898994696428 26.859217921317185 28.152457848492045 22.51642523549434 38-39 21.00846988047178 23.267434496952426 28.231615609910556 27.492480012665244 40-41 19.865629699992084 23.94522282909839 30.708264070292092 25.480883400617433 42-43 25.13357872239373 22.64208818174622 32.06087231853083 20.163460777329217 44-45 19.387714715427848 24.94854745507797 29.493192432518008 26.170545396976173 46-47 25.385894086915222 20.514723343623842 27.39056439483891 26.708818174622024 48-49 22.552046228132667 20.133776616797277 28.850035620992635 28.464141534077413 50-51 17.270244597482783 20.200071241985277 33.62522757856408 28.904456581967864 52-53 19.142325655030476 20.3870814533365 37.912609831393965 22.557983060239057 54-55 17.61458085965329 20.771986068233993 33.96560595266366 27.647827119449065 56-57 23.770086281959944 20.51373387160611 29.702960500277054 26.01321934615689 58-59 18.135043140979974 27.63100609514763 33.11861790548563 21.115332858386765 60-61 19.823082403229638 36.115728647193855 23.974906989630334 20.086281959946174 62-63 15.388268819757778 42.29201298187287 22.068194411462045 20.251523786907306 64-65 15.342753106942137 43.38636903348373 21.10444866619172 20.166429193382413 66-67 15.241826961133539 42.41767592812475 20.57409166468772 21.766405446053984 68-69 15.073616718119212 40.62969999208422 20.680954642602707 23.615728647193855 70-71 15.824625979577297 37.89380986305707 21.64470038787303 24.6368637694926 72-73 16.38763555766643 34.23771075753977 22.43429905802264 26.940354626771157 74-75 16.822013773450486 34.21693184516742 22.30269927966437 26.658355101717724 76 16.46283543101401 34.59787857199398 22.274994063167895 26.664291933824114 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 1.0 16 2.0 17 2.0 18 3.5 19 5.0 20 8.0 21 11.0 22 20.5 23 29.0 24 36.0 25 44.0 26 77.0 27 133.0 28 186.0 29 216.0 30 255.5 31 340.0 32 465.0 33 545.0 34 620.5 35 799.0 36 1018.0 37 1134.0 38 1292.0 39 1804.5 40 3029.5 41 4565.0 42 5230.0 43 5273.0 44 5038.5 45 4353.5 46 3946.0 47 3679.5 48 3117.0 49 2614.5 50 2408.0 51 2222.0 52 1863.5 53 1599.5 54 1508.0 55 1398.5 56 1123.0 57 854.5 58 752.0 59 666.0 60 510.5 61 405.0 62 369.0 63 317.5 64 242.0 65 187.0 66 134.0 67 112.0 68 102.0 69 67.5 70 33.5 71 24.0 72 16.5 73 10.0 74 7.0 75 3.0 76 2.5 77 2.0 78 1.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 50532.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.48484128868835 #Duplication Level Percentage of deduplicated Percentage of total 1 70.6871545607777 35.686297791498454 2 15.804946885657166 15.95820470197103 3 6.115009211712595 9.26145808596533 4 2.7203951236721413 5.493548642444392 5 1.6189094900239114 4.086519433230428 6 0.8545333385598369 2.5884587983851817 7 0.6781388420681275 2.396501226945302 8 0.43902630237936574 1.7731338557745588 9 0.26655168358747205 1.2111137497031583 >10 0.736937007565364 5.289717406791736 >50 0.03919877699815766 1.5178500751998734 >100 0.02743914389871036 2.899153011952822 >500 0.003919877699815766 1.8226074566611257 >1k 0.007839755399631531 10.015435763476608 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT 2573 5.091823003245468 No Hit ATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTT 2488 4.923612760231141 TruSeq Adapter, Index 9 (95% over 23bp) CTTTACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC 921 1.8226074566611257 RNA PCR Primer, Index 9 (95% over 21bp) CTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC 425 0.8410512150716377 TruSeq Adapter, Index 9 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG 246 0.4868202327238186 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGT 214 0.4234940235890129 No Hit TATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCT 208 0.4116203593762368 RNA PCR Primer, Index 9 (95% over 22bp) CTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC 138 0.27309427689384946 No Hit ATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGTCTT 122 0.24143117232644662 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC 112 0.22164173197181986 No Hit CTTAATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG 98 0.19393651547534235 No Hit ATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGTCTT 94 0.18602073933349164 No Hit CTTATAACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG 90 0.17810496319164093 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGT 89 0.17612601915617826 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGATCTGACCTCGTATGCCGT 85 0.16821024301432755 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC 71 0.14050502651785007 No Hit CTTAACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC 70 0.1385260824823874 TruSeq Adapter, Index 9 (95% over 21bp) CTTATCACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC 60 0.11873664212776064 TruSeq Adapter, Index 9 (95% over 21bp) ATACACATCTCCGAGCCCACGAGACTGGATCTGACCTCGTATGCCGTCTT 57 0.1127998100213726 No Hit TTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC 53 0.1048840338795219 TruSeq Adapter, Index 9 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.007915776141850708 0.0 2 0.0 0.0 0.0 0.009894720177313385 0.0 3 0.0 0.0 0.0 0.009894720177313385 0.0 4 0.0 0.0 0.0 0.009894720177313385 0.0 5 0.0 0.0 0.0 0.011873664212776063 0.0 6 0.0 0.0 0.0 0.011873664212776063 0.0 7 0.0 0.0 0.0 0.01385260824823874 0.0 8 0.0 0.0 0.0 0.01385260824823874 0.0 9 0.0 0.0 0.0 0.015831552283701415 0.0 10 0.0 0.0 0.0 0.01978944035462677 0.0 11 0.0 0.0 0.0 0.01978944035462677 0.0 12 0.0 0.0 0.0 0.01978944035462677 0.0 13 0.0 0.0 0.0 0.01978944035462677 0.0 14 0.0 0.0 0.0 0.02176838439008945 0.0 15 0.0 0.0 0.0 0.023747328425552126 0.0 16 0.0 0.0 0.0 0.025726272461014804 0.0 17 0.0 0.0 0.0 0.03166310456740283 0.0 18 0.0 0.0 0.0 0.035620992638328186 0.0 19 0.0 0.0 0.0 0.037599936673790864 0.0 20 0.0 0.0 0.0 0.04155782474471622 0.0 21 0.0 0.0 0.0 0.045515712815641575 0.0 22 0.0 0.0 0.0 0.04947360088656693 0.0 23 0.0 0.0 0.0 0.04947360088656693 0.0 24 0.0 0.0 0.0 0.057389377028417635 0.0 25 0.0 0.0 0.0 0.057389377028417635 0.0 26 0.0 0.0 0.0 0.06332620913480566 0.0 27 0.0 0.0 0.0 0.09894720177313386 0.0 28 0.0 0.0 0.0 0.11873664212776062 0.0 29 0.0 0.0 0.0 0.14248397055331274 0.0 30 0.0 0.0 0.0 0.17810496319164093 0.0 31 0.0 0.0 0.0 0.23549434022005858 0.0 32 0.0 0.0 0.0 0.29090477321301356 0.0 33 0.0 0.0 0.0 0.36610464656059527 0.0 34 0.0 0.0 0.0 0.45317818412095306 0.0 35 0.0 0.0 0.0 0.5679569381777884 0.0 36 0.0 0.0 0.0 0.692630412411937 0.0 37 0.0 0.0 0.0 1.0092614580859653 0.0 38 0.0 0.0 0.0 1.3001662312989788 0.0 39 0.0 0.0 0.0 1.6524182696113354 0.0 40 0.0 0.0 0.0 2.004670307923692 0.0 41 0.0 0.0 0.0 2.5172168131085253 0.0 42 0.0 0.0 0.0 3.1722472888466715 0.0 43 0.0 0.0 0.0 3.8866460856486977 0.0 44 0.0 0.0 0.0 4.555529169635083 0.0 45 0.0 0.0 0.0 5.190770205018602 0.0 46 0.0 0.0 0.0 5.8992321697142405 0.0 47 0.0 0.0 0.0 6.486978548246656 0.0 48 0.0 0.0 0.0 7.019314493786116 0.0 49 0.0 0.0 0.0 7.6011240402121425 0.0 50 0.0 0.0 0.0 8.052323280297633 0.0 51 0.0 0.0 0.0 8.584659225837093 0.0 52 0.0 0.0 0.0 9.144700387873032 0.0 53 0.0 0.0 0.0 9.86107812871052 0.0 54 0.0 0.0 0.0 10.624950526399113 0.0 55 0.0 0.0 0.0 11.432359692867886 0.0 56 0.0 0.0 0.0 12.04187445579039 0.0 57 0.0 0.0 0.0 12.679094435209372 0.0 58 0.0 0.0 0.0 13.193619884429669 0.0 59 0.0 0.0 0.0 13.71210322172089 0.0 60 0.0 0.0 0.0 14.189028734267396 0.0 61 0.0 0.0 0.0 14.67387002295575 0.0 62 0.0 0.0 0.0 15.259637457452703 0.0 63 0.0 0.0 0.0 15.756352410353836 0.0 64 0.0 0.0 0.0 16.363888229240878 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCATGG 15 0.0022125738 70.00001 1 TACCAGC 15 0.0022125738 70.00001 17 AAAAGCA 15 0.0022125738 70.00001 70 GTTAGGC 15 0.0022125738 70.00001 3 CCGTATG 15 0.0022125738 70.00001 40 CGAGGTG 15 0.0022125738 70.00001 26 AAATTCG 15 0.0022125738 70.00001 51 GGCATGT 15 0.0022125738 70.00001 29 AGCTCCG 15 0.0022125738 70.00001 21 TGAATTG 15 0.0022125738 70.00001 43 ATTGCAA 15 0.0022125738 70.00001 46 TTACCAG 15 0.0022125738 70.00001 16 TGTAAGG 15 0.0022125738 70.00001 12 CTTTACA 165 0.0 67.878784 1 TTTACAC 180 0.0 58.333336 2 CTATACA 70 0.0 55.0 1 CTTTATA 45 2.634988E-8 54.444447 2 GTGTTAG 20 0.006913804 52.5 27 TGGAGCT 20 0.006913804 52.5 25 CCTAGTA 20 0.006913804 52.5 2 >>END_MODULE