FastQCFastQC Report
Wed 25 May 2016
SRR1780355_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780355_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655105
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAC353.2008562E-431.4543135
CGTTACA502.335868E-630.83937614
GACGAGT450.001396468524.4476323
CGGTGGT2350.024.31243
CGTGCGA1301.8189894E-1223.69539510
CCGTGTA1254.0017767E-1122.90048834
AGCCGAC500.002546492722.04837618
CATAGGT500.002585251821.9910981
CATCGTT602.8906183E-421.97766532
CGACAAT956.7379005E-720.87993817
AGACTTA856.0994844E-620.7085811
TCAATCC654.8563594E-420.35234621
CGTTTAC654.886549E-420.33365414
TTGTTCG654.972949E-420.280889
TAGTCCC654.9755094E-420.2793315
ATACATC1001.1369593E-619.772356
TGTACGC1001.1377924E-619.7708424
CGAGAAT1452.8921932E-1019.74935314
GTACGCG901.0129597E-519.5282465
GACGACC1705.456968E-1219.41429523
Produced by FastQC (version 0.11.5)