FastQCFastQC Report
Wed 25 May 2016
SRR1780355_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780355_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655105
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT406446.20419627387976No Hit
ATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCTT341055.206035673670633No Hit
CTTTACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC108861.6617183504934325No Hit
CTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC60470.9230581357187015No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG46630.7117942925179933No Hit
TATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCT29370.4483250776593065No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC24070.36742201631799487No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC17580.2683539279962754No Hit
CTTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG15720.23996153288404148No Hit
CTTATAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG12340.18836675036826156No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC11100.16943848696010563No Hit
CTTAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC10240.15631082040283617No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC8000.12211782843971578No Hit
CTTATCACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC7440.11356958044893566No Hit
CTTATTACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG6840.104410743315957No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT207.852254E-444.0019876
CCGTCTT34850.042.16797644
GCCGTCT37350.039.28658743
CTATACA8050.037.1949351
CGCAAAG301.2989716E-436.6739212
CTTATAA1450.036.440471
TCGTCGG250.002353249835.19621334
CTTTACA13200.035.1924321
TTTACAC13350.034.6083032
TTACACA14600.031.6452623
TTATACA63850.029.7022022
CTTATAC64100.029.6753921
TCGCGCG300.005737568329.33913613
CGACTGC300.005741851429.3346566
ATTAACG454.0697163E-529.3301818
AGGCGAT454.0697163E-529.3301834
CGGGACA300.005746137429.3301818
TCTTTAT2700.028.5394131
CCGTTTA407.0205476E-427.50543813
CATCGTT655.9297963E-727.0740132
Produced by FastQC (version 0.11.5)