FastQCFastQC Report
Wed 25 May 2016
SRR1780354_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780354_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99448
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATTGG150.002218913370.0000143
CCAGTAG150.002218913370.0000121
CGTATCC200.006933462352.556
CCCGTAC200.006933462352.53
CACAGAT200.006933462352.569
GAACATA459.1413705E-538.8888926
CATGAGG400.002408852635.04
CGAACAT552.9739624E-431.8181825
GGTGGCA450.004289733231.1111139
TAGATCT703.459196E-530.00000239
TGTAGAT853.9113656E-628.8235337
GTAGATC755.537625E-528.038
CGACGAA808.592736E-526.2522
ATACCCA700.001218686325.0000024
CTTACTC1003.8988746E-421.012
TATACCC1107.411627E-419.0909083
TATATAC950.00716550718.4210513
GACGAAC1159.992264E-418.26086823
TCCGGTG1200.001329422617.50000270
GTGTAGA1602.568819E-517.536
Produced by FastQC (version 0.11.5)