##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780354_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 99448 Sequences flagged as poor quality 0 Sequence length 76 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.918138122435845 34.0 31.0 34.0 12.0 34.0 2 30.127855763816267 34.0 32.0 34.0 12.0 34.0 3 30.000713940954068 34.0 31.0 34.0 11.0 34.0 4 29.98412235540182 34.0 31.0 34.0 11.0 34.0 5 29.82823183975545 34.0 31.0 34.0 11.0 34.0 6 32.822097980854316 38.0 34.0 38.0 11.0 38.0 7 33.1050900973373 38.0 34.0 38.0 12.0 38.0 8 33.08435564315019 38.0 34.0 38.0 11.0 38.0 9 33.246118574531415 38.0 34.0 38.0 12.0 38.0 10-11 33.02189586517577 38.0 34.0 38.0 11.0 38.0 12-13 33.08769909902663 38.0 34.0 38.0 11.0 38.0 14-15 32.957621068297 38.0 34.0 38.0 11.0 38.0 16-17 32.97939123964283 38.0 34.0 38.0 11.0 38.0 18-19 33.00635508004183 38.0 34.0 38.0 11.0 38.0 20-21 33.054953342450325 38.0 34.0 38.0 11.0 38.0 22-23 33.02361535676937 38.0 34.0 38.0 11.0 38.0 24-25 33.01597317190894 38.0 34.0 38.0 11.0 38.0 26-27 33.00361495454911 38.0 34.0 38.0 11.0 38.0 28-29 33.042821373984395 38.0 34.0 38.0 11.0 38.0 30-31 33.03782881505913 38.0 34.0 38.0 11.0 38.0 32-33 33.11759411953986 38.0 34.0 38.0 11.0 38.0 34-35 33.05969451371571 38.0 34.0 38.0 11.0 38.0 36-37 32.97083400370043 38.0 34.0 38.0 11.0 38.0 38-39 32.963674483146974 38.0 34.0 38.0 11.0 38.0 40-41 33.03316306009171 38.0 34.0 38.0 11.0 38.0 42-43 33.09879032258065 38.0 34.0 38.0 11.0 38.0 44-45 33.15056612501006 38.0 34.0 38.0 11.0 38.0 46-47 33.06503398761161 38.0 34.0 38.0 11.0 38.0 48-49 33.012840881666804 38.0 34.0 38.0 11.0 38.0 50-51 32.94160767436248 38.0 34.0 38.0 11.0 38.0 52-53 33.006169053173515 38.0 34.0 38.0 11.0 38.0 54-55 32.97852143833964 38.0 34.0 38.0 11.0 38.0 56-57 32.9291438741855 38.0 34.0 38.0 11.0 38.0 58-59 32.94201995012469 38.0 34.0 38.0 11.0 38.0 60-61 32.98542454348001 38.0 33.5 38.0 11.0 38.0 62-63 32.854516933472766 38.0 33.0 38.0 11.0 38.0 64-65 32.81797019547905 38.0 33.0 38.0 11.0 38.0 66-67 32.73600273509774 38.0 32.5 38.0 11.0 38.0 68-69 32.68109967017939 38.0 32.0 38.0 11.0 38.0 70-71 32.62604074491192 38.0 32.0 38.0 11.0 38.0 72-73 32.636342611213905 38.0 32.0 38.0 11.0 38.0 74-75 32.59192743946585 38.0 32.0 38.0 11.0 38.0 76 30.948797361435123 36.0 26.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05961854660533561 1101 2 0.2364627235876462 1101 3 -0.4082608931495102 1101 4 0.2596068707760004 1101 5 0.22294159711190886 1101 6 -0.12011700756940513 1101 7 -0.30758026579671593 1101 8 -0.39245637268614786 1101 9 -0.7673861567868983 1101 10-11 -0.672630545946042 1101 12-13 -0.3611074172279416 1101 14-15 -0.1493195546709174 1101 16-17 -0.39157008547553573 1101 18-19 0.13157294225844396 1101 20-21 0.07701550416121705 1101 22-23 -0.10678497658001618 1101 24-25 -0.41613212593983917 1101 26-27 -0.5379145873949724 1101 28-29 -0.4906469081027396 1101 30-31 -0.21636528249720755 1101 32-33 0.19085143805565252 1101 34-35 -0.26937456404780136 1101 36-37 -0.01632552753104477 1101 38-39 0.05722685049553178 1101 40-41 -0.40751150352446075 1101 42-43 0.17012183633943323 1101 44-45 0.07888151718044867 1101 46-47 -0.09819297504780877 1101 48-49 -0.6331745690583617 1101 50-51 0.17057123537417596 1101 52-53 0.07047245765660648 1101 54-55 -0.28349010284306786 1101 56-57 -0.0418737398651885 1101 58-59 0.14490366697714308 1101 60-61 -0.14822540747147883 1101 62-63 -0.08984257992511857 1101 64-65 0.2386949007493797 1101 66-67 0.0713510950292573 1101 68-69 -0.4171024113578028 1101 70-71 -0.3841517492869855 1101 72-73 -0.13191325073611893 1101 74-75 -0.43781590295355954 1101 76 -1.4284482025134189 1102 1 0.4323240001810511 1102 2 0.6801935560690922 1102 3 0.6869877845694994 1102 4 0.6404460191825976 1102 5 0.0614233074101449 1102 6 0.8443036333479697 1102 7 0.3848282856944607 1102 8 0.47553776038643036 1102 9 0.6551659566403671 1102 10-11 0.1948413416934045 1102 12-13 0.4480952140001122 1102 14-15 0.04381125114267803 1102 16-17 0.07867303644099621 1102 18-19 0.03834657664219776 1102 20-21 0.4373204066013301 1102 22-23 0.6482152456520822 1102 24-25 0.2771764655531186 1102 26-27 -0.050948500013312525 1102 28-29 0.2944768751765139 1102 30-31 0.42340759629740177 1102 32-33 0.753965354229706 1102 34-35 0.4419014926658278 1102 36-37 0.5972461867673715 1102 38-39 0.4552667633805427 1102 40-41 0.528819000053474 1102 42-43 0.45135655788261886 1102 44-45 0.39318437508524795 1102 46-47 0.5766259057913459 1102 48-49 0.11818104674677699 1102 50-51 0.4024037612642104 1102 52-53 0.29170451241459006 1102 54-55 0.11518317152468427 1102 56-57 0.344743443120727 1102 58-59 0.26166441189914025 1102 60-61 0.018659787426074104 1102 62-63 0.07094234384927489 1102 64-65 0.496484135826492 1102 66-67 0.22180222619063983 1102 68-69 0.35194287954401204 1102 70-71 0.3044677449127562 1102 72-73 0.04209448294093221 1102 74-75 -0.1873936619650678 1102 76 0.42207848756169497 1103 1 -0.18169344511112584 1103 2 0.006691599485570521 1103 3 -0.16389650373690756 1103 4 0.5572857542240222 1103 5 -0.4801021718981673 1103 6 -0.4653830946759854 1103 7 -0.1159587199676082 1103 8 -0.6085078859419681 1103 9 0.0912436078656853 1103 10-11 0.21577897149468583 1103 12-13 0.2681988364122816 1103 14-15 -0.10963299169230112 1103 16-17 0.06228801578209442 1103 18-19 0.37009678978956373 1103 20-21 -0.2534847439135106 1103 22-23 -0.0949367120244915 1103 24-25 -0.5159120944451345 1103 26-27 -0.4337447748531815 1103 28-29 -0.06054465817284438 1103 30-31 -0.40346755399881573 1103 32-33 -0.2521776621881031 1103 34-35 -0.48826815077688224 1103 36-37 -0.34590358478236993 1103 38-39 -0.37280529136007345 1103 40-41 -0.22520893864752622 1103 42-43 0.06585495107938755 1103 44-45 0.1494749550646972 1103 46-47 -0.05908884837869266 1103 48-49 -0.11446110615093374 1103 50-51 -0.8607239304044612 1103 52-53 0.10497102313754425 1103 54-55 0.14197962125469843 1103 56-57 -0.06309491466079464 1103 58-59 -0.13276205772462646 1103 60-61 0.08943017852416801 1103 62-63 -0.2140848734298757 1103 64-65 0.006523923334960102 1103 66-67 0.019093615037022005 1103 68-69 0.08503318844373808 1103 70-71 0.15367964618726404 1103 72-73 -0.020570842901271646 1103 74-75 -0.032790771905389704 1103 76 0.33434921750944824 1104 1 0.28981550196785477 1104 2 -0.11693884173444857 1104 3 0.05740092847257472 1104 4 0.7409864183807144 1104 5 -0.14978997595079235 1104 6 0.4785971921381744 1104 7 0.6436969338248559 1104 8 -0.32100629958044635 1104 9 -0.221544581144002 1104 10-11 0.32084369504475774 1104 12-13 0.08381603680146554 1104 14-15 0.17022762000870983 1104 16-17 0.3025900590157349 1104 18-19 0.36265146638332624 1104 20-21 0.008650921723791782 1104 22-23 0.18628288271301585 1104 24-25 0.3999102753674606 1104 26-27 0.2740209935803435 1104 28-29 0.07931954928093887 1104 30-31 0.45821758496645515 1104 32-33 0.18529325451733314 1104 34-35 0.3915332367606581 1104 36-37 0.10212721178262285 1104 38-39 -9.674386590248218E-4 1104 40-41 0.4478789669322367 1104 42-43 0.20027793851541276 1104 44-45 0.3629939279484944 1104 46-47 0.48494325957849327 1104 48-49 -0.08968742706128552 1104 50-51 0.03703506955323377 1104 52-53 -0.02657536207333777 1104 54-55 -0.27685683544820705 1104 56-57 0.41163963417798755 1104 58-59 0.014409988470212909 1104 60-61 0.13148991032331026 1104 62-63 0.035576702778932656 1104 64-65 0.4895657186731768 1104 66-67 0.2783262390904966 1104 68-69 0.4156467930801355 1104 70-71 0.29421541335169366 1104 72-73 0.2746404604533552 1104 74-75 0.05345441613133062 1104 76 0.24627442585159187 1105 1 -0.361620019032884 1105 2 -0.11161807554411496 1105 3 0.07263569370734047 1105 4 0.8594983412404638 1105 5 0.185820066180991 1105 6 0.05646497652769966 1105 7 0.2459425143313041 1105 8 0.07575997762061348 1105 9 0.37789945308264095 1105 10-11 0.386750614212545 1105 12-13 0.19736010143249416 1105 14-15 0.020545780109479495 1105 16-17 0.1957186695356512 1105 18-19 0.11205423752522847 1105 20-21 -0.09429504535261657 1105 22-23 0.04912656077408428 1105 24-25 -0.02623423229878341 1105 26-27 0.059784143473926576 1105 28-29 -0.11614802662141699 1105 30-31 -0.0679899031975495 1105 32-33 0.00391485031283878 1105 34-35 0.36346890978328616 1105 36-37 -0.5581038614919294 1105 38-39 0.14154765494302524 1105 40-41 0.08419373911657146 1105 42-43 -0.22751709254049501 1105 44-45 0.08890388755410328 1105 46-47 -0.14101230659446173 1105 48-49 -0.010923799384620736 1105 50-51 0.31292765522842814 1105 52-53 -0.25412498636208625 1105 54-55 -0.013495197086569988 1105 56-57 0.16016285009251163 1105 58-59 -0.026179856467457796 1105 60-61 0.043827572660973146 1105 62-63 -0.314974919511819 1105 64-65 -0.6446967579806042 1105 66-67 -0.1740474741527862 1105 68-69 -0.2465117006000881 1105 70-71 -0.066320789575812 1105 72-73 -0.06371683442567644 1105 74-75 -0.19168088335179334 1105 76 -0.6890076833435579 1106 1 -0.06383429650952976 1106 2 0.23886053130630458 1106 3 -0.13532186597435825 1106 4 0.34053859034667866 1106 5 0.5974334902381919 1106 6 0.5357176578793315 1106 7 0.45108609638643316 1106 8 0.05232554099276854 1106 9 0.31455035839921663 1106 10-11 -0.3777344509471874 1106 12-13 0.4127793149026786 1106 14-15 0.47019403290615713 1106 16-17 0.46842078001887444 1106 18-19 0.5252130427380095 1106 20-21 0.5084382034076214 1106 22-23 0.4310432947148044 1106 24-25 0.2004812561108622 1106 26-27 0.6477670750850422 1106 28-29 0.33142434104090057 1106 30-31 0.24727938523515292 1106 32-33 0.5526577865370399 1106 34-35 0.41469714182111517 1106 36-37 0.3717196882716465 1106 38-39 0.27911540952727165 1106 40-41 0.8255211523330388 1106 42-43 0.44054887447600066 1106 44-45 0.3650427412042063 1106 46-47 0.49590385071149967 1106 48-49 0.586521708630606 1106 50-51 0.07146184966966729 1106 52-53 0.9248912747381297 1106 54-55 0.2713854395182054 1106 56-57 0.5568702656155224 1106 58-59 0.7220902392539159 1106 60-61 0.650724124385107 1106 62-63 0.18857715060696734 1106 64-65 0.42994861164227416 1106 66-67 0.3989487200578026 1106 68-69 0.1191958302498719 1106 70-71 0.5335235190391145 1106 72-73 0.7114071420476193 1106 74-75 0.6721064537488886 1106 76 0.7624858006836561 1107 1 -0.01044945047101109 1107 2 0.02216118866993 1107 3 -0.19826731632610262 1107 4 -1.0301337657829492 1107 5 -0.5365879605748631 1107 6 -1.0171136187900274 1107 7 -0.14201735188942877 1107 8 -0.0412968785666763 1107 9 -0.4649099114658526 1107 10-11 -0.15003868344631854 1107 12-13 0.03682498437563453 1107 14-15 -0.1149057249072456 1107 16-17 -0.06180641407638632 1107 18-19 -0.10032034441456972 1107 20-21 0.14316314862731616 1107 22-23 -0.13983664992825595 1107 24-25 0.21122396168114932 1107 26-27 0.18520220367459928 1107 28-29 -0.13287043464818282 1107 30-31 -0.3388929132309997 1107 32-33 -0.002774112897860448 1107 34-35 -0.15659797984881862 1107 36-37 0.13420383081576404 1107 38-39 -0.0959105390369075 1107 40-41 0.48887601336406306 1107 42-43 0.30425548271034586 1107 44-45 0.30295071029657805 1107 46-47 0.2201917067834671 1107 48-49 0.06432703673578288 1107 50-51 0.17747615021171015 1107 52-53 0.050222839724867185 1107 54-55 0.2958693514084061 1107 56-57 -0.41843246763324515 1107 58-59 0.18117800306431775 1107 60-61 -0.19864734372698933 1107 62-63 -0.17283445128439467 1107 64-65 0.1613233089090258 1107 66-67 -0.0018066714772047021 1107 68-69 -0.27828427585092896 1107 70-71 0.12933138433723457 1107 72-73 -0.12893422573003477 1107 74-75 -0.15154710408757666 1107 76 0.11366790194072252 1108 1 0.09532706357697762 1108 2 0.3187596061591229 1108 3 0.19083456800377618 1108 4 -0.009251148930022879 1108 5 -0.25265662150036405 1108 6 0.7265382754451153 1108 7 -0.26396857140162666 1108 8 -0.14861162108820736 1108 9 0.2577078737182106 1108 10-11 0.07397166463614013 1108 12-13 0.45564847265829655 1108 14-15 -0.062390371755626006 1108 16-17 -0.37590218045211543 1108 18-19 -0.045014038843156357 1108 20-21 -0.05925373459550087 1108 22-23 0.06773679465472782 1108 24-25 0.2507575987757633 1108 26-27 0.45358228594650996 1108 28-29 0.19881590658216908 1108 30-31 0.18893503824488533 1108 32-33 -0.07907956778231551 1108 34-35 -0.07564287980569873 1108 36-37 -0.18342869572450127 1108 38-39 0.11318365415060327 1108 40-41 0.40642347120844136 1108 42-43 0.5082047677836954 1108 44-45 0.07359734891777236 1108 46-47 -0.047722245647989325 1108 48-49 0.026285015130660838 1108 50-51 0.037549798726502104 1108 52-53 -0.1482494197230011 1108 54-55 0.35812099160278876 1108 56-57 -0.04868662492875586 1108 58-59 0.6021515152361374 1108 60-61 0.561249175448431 1108 62-63 0.26878861282011 1108 64-65 0.4837453195600929 1108 66-67 0.43939687577088904 1108 68-69 -0.12430410967564143 1108 70-71 0.29583878997507185 1108 72-73 0.33482417917365837 1108 74-75 0.6770686068186862 1108 76 0.2038290663330642 1109 1 -0.07793418606653191 1109 2 -0.1070714848919998 1109 3 -0.2569564223616432 1109 4 -0.7917353082911909 1109 5 -0.3489280265123291 1109 6 -0.0788853608959812 1109 7 -0.2558963554481508 1109 8 -0.3818627029635451 1109 9 -0.8987641587194446 1109 10-11 -0.48834311458689683 1109 12-13 -0.3704578921165904 1109 14-15 0.19063825919158717 1109 16-17 -0.28767199210041383 1109 18-19 -0.13658595100356052 1109 20-21 -0.2808242827870018 1109 22-23 0.06162373403250854 1109 24-25 -0.780700483979949 1109 26-27 -0.429432581314515 1109 28-29 -0.28130114674901563 1109 30-31 -0.541811300738587 1109 32-33 -0.6018826478720882 1109 34-35 -0.3759888319179865 1109 36-37 -0.47163532461336644 1109 38-39 -0.2542199690467868 1109 40-41 -0.33406284570352796 1109 42-43 0.03575073973554055 1109 44-45 -0.18988424370192547 1109 46-47 -0.24630991784906087 1109 48-49 0.09946194669662844 1109 50-51 -0.22105870499961355 1109 52-53 -0.3614935577566527 1109 54-55 -0.2973714631692417 1109 56-57 -0.4800851890414961 1109 58-59 -0.020853680245409123 1109 60-61 -0.34585214264151887 1109 62-63 -0.3200987605477721 1109 64-65 -0.4852863865170107 1109 66-67 -0.3401051515828897 1109 68-69 -0.4952292525823836 1109 70-71 -0.08977381805746631 1109 72-73 -0.4460232175056973 1109 74-75 -0.13653264307281177 1109 76 0.13258029080514433 1110 1 -0.014080601928249337 1110 2 0.12494427514867112 1110 3 -0.11857309750145006 1110 4 0.02994745734175197 1110 5 0.09176846560765028 1110 6 0.3841166726576546 1110 7 0.03298264811056839 1110 8 0.17769499912085251 1110 9 0.44890996431054475 1110 10-11 -0.3064290322279746 1110 12-13 -0.11813201514658545 1110 14-15 -0.2518503020692151 1110 16-17 -0.1175398111141277 1110 18-19 -0.32484853361666666 1110 20-21 -0.11034258476971814 1110 22-23 -0.30430153287140627 1110 24-25 0.15559209354928072 1110 26-27 -0.029388097328748586 1110 28-29 0.022988380449767476 1110 30-31 0.11876953301840132 1110 32-33 -0.23613531691123057 1110 34-35 -0.1737265034990756 1110 36-37 0.17079604295145856 1110 38-39 -0.030675230866819447 1110 40-41 -0.14449116293789643 1110 42-43 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-0.6288150317203431 2116 20-21 0.0506719537890703 2116 22-23 -0.26248737357936847 2116 24-25 -0.22580803287296902 2116 26-27 -0.17640137166502967 2116 28-29 -0.526616550017998 2116 30-31 -0.1305509473861477 2116 32-33 -0.6925512461147747 2116 34-35 -0.18864432903637152 2116 36-37 -0.4010908091345087 2116 38-39 -0.4219678983763018 2116 40-41 -0.7924526041515492 2116 42-43 -0.3393870425212526 2116 44-45 -0.31234511354099226 2116 46-47 0.020499517479890983 2116 48-49 -0.5306281235329564 2116 50-51 -0.10537672219484051 2116 52-53 -0.5374218165038585 2116 54-55 0.00981998330825462 2116 56-57 -0.04839369532437132 2116 58-59 -0.6157187323664672 2116 60-61 -0.32785384326822964 2116 62-63 0.13743281127221252 2116 64-65 -0.7188155018761861 2116 66-67 -0.7478776455204681 2116 68-69 -0.12358720301226356 2116 70-71 -0.5281182264598172 2116 72-73 -0.15964870171191592 2116 74-75 -0.6440516176578868 2116 76 -0.17764236532183375 2117 1 0.12601156397036917 2117 2 -0.08070088614165272 2117 3 0.6224407273833421 2117 4 0.08179077531410428 2117 5 0.4956855162988951 2117 6 0.24367888463922327 2117 7 0.25890430709674916 2117 8 0.200045229535597 2117 9 0.47598369702944154 2117 10-11 0.3706447004909279 2117 12-13 -0.060873950630451645 2117 14-15 0.16577642604138276 2117 16-17 -0.23343842862566078 2117 18-19 -0.019837012879342808 2117 20-21 0.14328644748834307 2117 22-23 0.0852145961608528 2117 24-25 0.32696306129121666 2117 26-27 0.24013955244083007 2117 28-29 0.37016579980460307 2117 30-31 0.5024676897004312 2117 32-33 0.362136572489689 2117 34-35 0.6656744181599024 2117 36-37 0.27430986783624434 2117 38-39 -0.04490334146589703 2117 40-41 0.17803187853675695 2117 42-43 0.1271212564877615 2117 44-45 0.694424594056585 2117 46-47 0.2908188356153687 2117 48-49 0.08726062488675979 2117 50-51 0.20434759573672778 2117 52-53 0.6209424427025354 2117 54-55 0.5458809106766367 2117 56-57 -0.06835617646298431 2117 58-59 0.5003756357261935 2117 60-61 -0.1036378651270411 2117 62-63 0.12177276478144705 2117 64-65 0.03454896123706419 2117 66-67 0.6138938972387962 2117 68-69 -0.03528533930360567 2117 70-71 -0.3907971547253837 2117 72-73 -0.7337382576027878 2117 74-75 0.05376861805843447 2117 76 0.5713686864297252 2118 1 -0.019113910588501426 2118 2 -0.3409143480933743 2118 3 0.7521893007220868 2118 4 -0.7333760067589132 2118 5 0.4581267187534692 2118 6 -0.45520605125056335 2118 7 0.1740448242763719 2118 8 0.4652967755827291 2118 9 0.14108242915140323 2118 10-11 -0.8983794393338158 2118 12-13 -0.12014903957293654 2118 14-15 -0.49028479503738964 2118 16-17 -0.04644432628808204 2118 18-19 0.04325842419235926 2118 20-21 -0.3058551680413686 2118 22-23 -0.3704932427159662 2118 24-25 0.30349179747230437 2118 26-27 -0.2734943149453315 2118 28-29 -0.6170837883141118 2118 30-31 -0.035532835597436474 2118 32-33 -0.14844101639310026 2118 34-35 -0.18560659232216636 2118 36-37 0.20716724946515086 2118 38-39 -0.030684483272445107 2118 40-41 -0.03330500453671448 2118 42-43 0.0769433715279888 2118 44-45 0.4055465825477853 2118 46-47 0.3732667236791656 2118 48-49 -0.07484152167133828 2118 50-51 0.00915168350923068 2118 52-53 0.21821805694288088 2118 54-55 -0.21975267018341782 2118 56-57 0.09037928830624509 2118 58-59 -0.29340913541108193 2118 60-61 -0.28777864495560834 2118 62-63 -0.1369722508581006 2118 64-65 -0.20364974462000873 2118 66-67 -0.17352760654573984 2118 68-69 0.2948524320008019 2118 70-71 0.07341095873895398 2118 72-73 -0.6291681661531996 2118 74-75 -0.4485474679949064 2118 76 0.7928056148698275 2119 1 0.3637144291276506 2119 2 0.4991678776331412 2119 3 -0.009722165824431528 2119 4 0.02839466231069565 2119 5 0.7234454842411893 2119 6 0.5142408962601408 2119 7 0.8579826481105712 2119 8 0.5561111481891743 2119 9 -0.31273599842237587 2119 10-11 0.41538773795836903 2119 12-13 0.3261226432385058 2119 14-15 0.37004410786867226 2119 16-17 0.7030347230473524 2119 18-19 0.6720458763212136 2119 20-21 0.4815828810687961 2119 22-23 0.3223289019112059 2119 24-25 0.42150824261760533 2119 26-27 0.6450684244103826 2119 28-29 0.1915442810708896 2119 30-31 0.5607726386084622 2119 32-33 0.4317994656974591 2119 34-35 0.6726244281779401 2119 36-37 0.4709513224545603 2119 38-39 0.6749924709965356 2119 40-41 0.740757284267076 2119 42-43 0.27791769006820743 2119 44-45 0.2774490380557779 2119 46-47 0.33975692417476466 2119 48-49 0.5457233578060183 2119 50-51 0.46426544787057367 2119 52-53 0.6756408999255328 2119 54-55 0.44469878284653674 2119 56-57 0.8127548233366539 2119 58-59 -0.015728351450732703 2119 60-61 0.5275232248348871 2119 62-63 0.4751588597523906 2119 64-65 0.29698384402608013 2119 66-67 -0.2894320895373994 2119 68-69 0.03902341645676444 2119 70-71 0.09086984587569447 2119 72-73 0.706141025106092 2119 74-75 0.3731184477518852 2119 76 0.560574256455773 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 17.0 13 280.0 14 1068.0 15 1944.0 16 2458.0 17 2811.0 18 2701.0 19 2318.0 20 1814.0 21 1271.0 22 903.0 23 696.0 24 597.0 25 602.0 26 611.0 27 694.0 28 844.0 29 1004.0 30 1315.0 31 1660.0 32 2161.0 33 2978.0 34 4207.0 35 6849.0 36 13370.0 37 44275.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.96456439546296 18.752513876598826 14.135025339876117 29.1478963880621 2 22.098986404955355 18.615758989622716 30.555667283404393 28.729587322017537 3 19.907891561419035 22.058764379374146 25.611374788834368 32.42196927037246 4 16.470919475504786 17.32262086718687 27.037245595688198 39.169214061620146 5 19.156745233689968 29.19012951492237 28.809025822540423 22.844099428847237 6 31.80255007642185 30.34148499718446 15.640334647252836 22.215630279140857 7 29.43246721904915 26.520392566969676 17.3135709114311 26.733569302550077 8 25.529925187032422 31.202236344622314 17.300498753117207 25.96733971522806 9 23.92506636634221 17.9993564475907 17.929973453463116 40.14560373260397 10-11 28.023187997747566 21.8576542514681 21.71536883597458 28.403788914809752 12-13 26.066386453221785 19.412657871450406 22.184458209315423 32.33649746601239 14-15 25.627966374386613 20.120565521679673 20.26335371249296 33.98811439144075 16-17 27.103611937897192 21.701291127021157 20.93305043842008 30.262046496661572 18-19 26.379615477435443 20.795792776124205 21.518783685946424 31.305808060493927 20-21 26.05884482342531 20.847075858740247 22.017536803153405 31.076542514681037 22-23 27.590298447429813 20.864672994932025 20.938580966937494 30.606447590700668 24-25 26.95680154452578 21.84860429571233 20.022524334325475 31.17206982543641 26-27 25.611374788834368 22.340821333762367 21.750563108358136 30.297240769045132 28-29 26.556089614672995 21.291529241412597 22.64650872817955 29.505872415734856 30-31 26.7159721663583 20.817412114874102 22.68572520312123 29.78089051564637 32-33 25.28557638162658 21.787768482020756 22.978843214544284 29.94781192180838 34-35 25.712432628107152 21.681180114230553 22.5766229587322 30.029764298930097 36-37 25.97337301906524 20.487591505108195 23.201572681200226 30.337462794626337 38-39 25.154352023167885 20.576079961386856 25.014077708953426 29.255490306491833 40-41 24.963297401657147 20.212573405196686 25.113627222266917 29.710501970879257 42-43 23.816466897272946 20.089896227174002 24.94871691738396 31.144919958169094 44-45 22.8878408816668 20.11553776848202 26.792896790282363 30.20372455956882 46-47 23.046215107392808 20.895342289437696 26.385648781272625 29.67279382189687 48-49 23.011523610329014 21.467500603330382 25.57416941517175 29.946806371168854 50-51 22.567070227656664 22.372496178907568 24.385105783927276 30.675327809508484 52-53 21.907931783444614 22.36847397634945 24.70436811197812 31.019226128227817 54-55 22.131666800740085 21.703805003619983 24.778276083983588 31.386252111656344 56-57 22.387579438500524 22.13116402542032 24.425327809508488 31.05592872657067 58-59 22.658689258151284 21.64760300661153 24.296236707810653 31.397471027426533 60-61 22.550981417424182 20.93807819161773 24.515827367066205 31.995113023891886 62-63 22.834043922451936 21.472528356528034 23.826019628348483 31.867408092671546 64-65 22.989904271579118 21.52833641702196 23.086939908293783 32.39481940310514 66-67 23.068839996782238 22.65203925669697 21.508728179551124 32.770392566969676 68-69 22.902924141259753 22.889349207626097 21.732463196846595 32.475263454267555 70-71 24.009532620062746 22.650028155417907 21.82095165312525 31.519487571394095 72-73 24.01958812645805 22.867729868876197 21.452920119057197 31.65976188560856 74-75 23.78127262488939 22.3518823907972 22.281493846030088 31.585351138283325 76 23.473574129193146 21.320690209958972 21.467500603330382 33.7382350575175 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.5 14 1.0 15 3.5 16 6.0 17 6.0 18 4.5 19 10.5 20 19.5 21 21.0 22 29.0 23 45.0 24 66.5 25 80.0 26 122.0 27 193.0 28 281.5 29 341.0 30 392.5 31 550.5 32 790.0 33 923.0 34 1041.5 35 1319.5 36 1652.0 37 1825.0 38 1961.5 39 2431.5 40 3039.5 41 3431.5 42 3549.0 43 3652.5 44 3909.5 45 4067.0 46 4071.0 47 4123.5 48 3977.5 49 3638.0 50 3497.0 51 3241.0 52 2778.5 53 2431.5 54 2291.0 55 2252.0 56 2027.0 57 1878.0 58 1915.0 59 1976.0 60 2162.5 61 2488.5 62 2689.0 63 2801.5 64 3065.0 65 3288.5 66 3456.5 67 3552.0 68 3596.5 69 3593.5 70 3479.5 71 3413.0 72 3272.5 73 2944.0 74 2535.5 75 2315.0 76 1988.0 77 1433.0 78 949.0 79 693.0 80 536.0 81 273.5 82 111.0 83 54.0 84 37.5 85 15.5 86 12.5 87 15.0 88 12.0 89 8.0 90 5.0 91 2.5 92 2.0 93 4.0 94 4.0 95 2.5 96 3.0 97 3.5 98 3.5 99 9.5 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.027753197651034E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 99448.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.70328211728743 #Duplication Level Percentage of deduplicated Percentage of total 1 77.68122537795287 54.92317593113989 2 13.693058182697367 19.36288311479366 3 4.6719667771251405 9.909701552570187 4 1.7550097421529447 4.9633979567211 5 0.8789270831851862 3.107151476148339 6 0.44373017791873476 1.882390797200547 7 0.27733136119920926 1.3725766229587322 8 0.18630978624152006 1.0538170702276566 9 0.11235475658839758 0.714946504705977 >10 0.3000867549386316 2.709958973533907 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0010055506395302068 0.0 3 0.0 0.0 0.0 0.0010055506395302068 0.0 4 0.0 0.0 0.0 0.013072158313892688 0.0 5 0.0 0.0 0.0 0.015083259592953101 0.0 6 0.0 0.0 0.0 0.015083259592953101 0.0 7 0.0 0.0 0.0 0.015083259592953101 0.0 8 0.0 0.0 0.0 0.015083259592953101 0.0 9 0.0 0.0 0.0 0.01809991151154372 0.0 10 0.0 0.0 0.0 0.022122114069664547 0.0 11 0.0 0.0 0.0 0.022122114069664547 0.0 12 0.0 0.0 0.0 0.022122114069664547 0.0 13 0.0 0.0 0.0 0.022122114069664547 0.0 14 0.0 0.0 0.0 0.022122114069664547 0.0 15 0.0 0.0 0.0 0.023127664709194754 0.0 16 0.0 0.0 0.0 0.02413321534872496 0.0 17 0.0 0.0 0.0 0.026144316627785377 0.0 18 0.0 0.0 0.0 0.028155417906845788 0.0 19 0.0 0.0 0.0 0.034188721744027026 0.0 20 0.0 0.0 0.0 0.04424422813932909 0.0 21 0.0 0.0 0.0 0.047260880057919716 0.0 22 0.0 0.0 0.0 0.05832193709275199 0.0 23 0.0 0.0 0.0 0.06636634220899365 0.0 24 0.0 0.0 0.0 0.0734051966857051 0.0 25 0.0 0.0 0.0 0.08446625372053737 0.0 26 0.0 0.0 0.0 0.09954951331349046 0.0 27 0.0 0.0 0.0 0.17194915935966534 0.0 28 0.0 0.0 0.0 0.26043761563832357 0.0 29 0.0 0.0 0.0 0.3871369962191296 0.0 30 0.0 0.0 0.0 0.48266430697449925 0.0 31 0.0 0.0 0.0 0.6244469471482584 0.0 32 0.0 0.0 0.0 0.7742739924382592 0.0 33 0.0 0.0 0.0 0.9552731075536964 0.0 34 0.0 0.0 0.0 1.1955997104014158 0.0 35 0.0 0.0 0.0 1.643069744992358 0.0 36 0.0 0.0 0.0 2.2242780146408174 0.0 37 0.0 0.0 0.0 2.902019145684177 0.0 38 0.0 0.0 0.0 3.5878046818437777 0.0 39 0.0 0.0 0.0 4.28062907248009 0.0 40 0.0 0.0 0.0 4.978481216314053 0.0 41 0.0 0.0 0.0 5.6974499235781515 0.0 42 0.0 0.0 0.0 6.493846030086075 0.0 43 0.0 0.0 0.0 7.313369801303193 0.0 44 0.0 0.0 0.0 8.07155498350897 0.0 45 0.0 0.0 0.0 8.729185101761725 0.0 46 0.0 0.0 0.0 9.461225967339715 0.0 47 0.0 0.0 0.0 10.088689566406565 0.0 48 0.0 0.0 0.0 10.725203121229185 0.0 49 0.0 0.0 0.0 11.249095004424422 0.0 50 0.0 0.0 0.0 11.782036843375433 0.0 51 0.0 0.0 0.0 12.346150752151878 0.0 52 0.0 0.0 0.0 13.060091706218325 0.0 53 0.0 0.0 0.0 13.829337945458933 0.0 54 0.0 0.0 0.0 14.536240045048668 0.0 55 0.0 0.0 0.0 15.22001447992921 0.0 56 0.0 0.0 0.0 15.76200627463599 0.0 57 0.0 0.0 0.0 16.339192341726328 0.0 58 0.0 0.0 0.0 16.88822299090982 0.0 59 0.0 0.0 0.0 17.384965006837746 0.0 60 0.0 0.0 0.0 17.919917947067816 0.0 61 0.0 0.0 0.0 18.464926393693187 0.0 62 0.0 0.0 0.0 19.038090258225406 0.0 63 0.0 0.0 0.0 19.629354034269166 0.0 64 0.0 0.0 0.0 20.24273992438259 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAATTGG 15 0.0022189133 70.00001 43 CCAGTAG 15 0.0022189133 70.00001 21 CGTATCC 20 0.0069334623 52.5 56 CCCGTAC 20 0.0069334623 52.5 3 CACAGAT 20 0.0069334623 52.5 69 GAACATA 45 9.1413705E-5 38.88889 26 CATGAGG 40 0.0024088526 35.0 4 CGAACAT 55 2.9739624E-4 31.81818 25 GGTGGCA 45 0.0042897332 31.111113 9 TAGATCT 70 3.459196E-5 30.000002 39 TGTAGAT 85 3.9113656E-6 28.82353 37 GTAGATC 75 5.537625E-5 28.0 38 CGACGAA 80 8.592736E-5 26.25 22 ATACCCA 70 0.0012186863 25.000002 4 CTTACTC 100 3.8988746E-4 21.0 12 TATACCC 110 7.411627E-4 19.090908 3 TATATAC 95 0.007165507 18.421051 3 GACGAAC 115 9.992264E-4 18.260868 23 TCCGGTG 120 0.0013294226 17.500002 70 GTGTAGA 160 2.568819E-5 17.5 36 >>END_MODULE