SRR1780354_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1780354_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	99448
Sequences flagged as poor quality	0
Sequence length	76
%GC	54

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT	7773	7.816145121068296	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	2116	2.1277451532459173	No Hit
CACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTTCTG	2108	2.119700748129676	RNA PCR Primer, Index 15 (96% over 25bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	1721	1.730552650631486	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	575	0.5781916177298688	No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT	333	0.33484836296355885	RNA PCR Primer, Index 48 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT	197	0.19809347598745072	No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	145	0.14580484273187996	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	142	0.14278819081328936	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAACTCGTATGCCGT	137	0.13776043761563833	No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	126	0.12669938058080607	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT	117	0.11764942482503418	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTTAT	90	0.0	66.111115	1
GTCAAGC	30	8.324141E-6	58.333336	33
CCTTATA	25	2.3803893E-4	55.999996	2
CTTTATA	100	0.0	55.999996	2
GTCGCTC	20	0.0069334623	52.5	46
GGAGCTT	20	0.0069334623	52.5	70
AACTAAA	20	0.0069334623	52.5	70
TCCTTAT	20	0.0069334623	52.5	1
TTTATAC	115	0.0	51.73913	3
CGTCAAG	35	2.0736094E-5	50.000004	32
TCAAGCT	40	4.5644338E-5	43.75	34
GACAGGG	35	0.0012502433	40.000004	41
GCTCGTA	45	9.1413705E-5	38.88889	38
CTTAATA	40	0.0024088526	35.0	2
ATCGTAT	50	1.6992886E-4	35.0	39
GTCTTCT	1745	0.0	33.295128	49
CCGTCTT	1750	0.0	33.2	47
TCTTCTG	1755	0.0	33.105415	50
TCTGCTT	1745	0.0	33.094555	53
ATGCCGT	1770	0.0	33.0226	44