##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780354_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 99448 Sequences flagged as poor quality 0 Sequence length 76 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.595115034993164 34.0 34.0 34.0 34.0 34.0 2 33.654462633738234 34.0 34.0 34.0 34.0 34.0 3 33.685514037486925 34.0 34.0 34.0 34.0 34.0 4 33.69167806290725 34.0 34.0 34.0 34.0 34.0 5 33.674804923175934 34.0 34.0 34.0 34.0 34.0 6 37.34238999276003 38.0 38.0 38.0 36.0 38.0 7 37.35939385407449 38.0 38.0 38.0 36.0 38.0 8 37.223855683372214 38.0 38.0 38.0 36.0 38.0 9 37.28155417906846 38.0 38.0 38.0 36.0 38.0 10-11 37.21780226852225 38.0 38.0 38.0 36.0 38.0 12-13 37.18143652964363 38.0 38.0 38.0 36.0 38.0 14-15 37.09723171908937 38.0 38.0 38.0 36.0 38.0 16-17 37.113129474700344 38.0 38.0 38.0 36.0 38.0 18-19 37.03894497626901 38.0 38.0 38.0 35.5 38.0 20-21 37.1070207545652 38.0 38.0 38.0 36.0 38.0 22-23 37.08208309870486 38.0 38.0 38.0 35.5 38.0 24-25 37.080795993886255 38.0 38.0 38.0 35.5 38.0 26-27 36.93827427399244 38.0 38.0 38.0 35.0 38.0 28-29 36.91276848202075 38.0 38.0 38.0 35.0 38.0 30-31 36.90023932105221 38.0 37.5 38.0 35.0 38.0 32-33 36.80891018421688 38.0 37.0 38.0 34.5 38.0 34-35 36.79486766953584 38.0 37.0 38.0 34.0 38.0 36-37 36.78684840318559 38.0 37.0 38.0 34.5 38.0 38-39 36.04607433030327 38.0 37.0 38.0 33.5 38.0 40-41 36.413582978038775 38.0 37.0 38.0 34.0 38.0 42-43 36.70332736706621 38.0 37.0 38.0 34.0 38.0 44-45 36.81061459255088 38.0 37.0 38.0 34.5 38.0 46-47 36.73169395060735 38.0 37.0 38.0 34.5 38.0 48-49 36.75750643552409 38.0 37.0 38.0 34.5 38.0 50-51 36.54321353873381 38.0 37.0 38.0 34.0 38.0 52-53 36.55098141742418 38.0 37.0 38.0 34.0 38.0 54-55 36.5207646207063 38.0 37.0 38.0 34.0 38.0 56-57 36.56951371571073 38.0 37.0 38.0 34.0 38.0 58-59 36.41611696565039 38.0 37.0 38.0 34.0 38.0 60-61 36.37736304400289 38.0 37.0 38.0 34.0 38.0 62-63 36.37428103129274 38.0 37.0 38.0 34.0 38.0 64-65 36.27765264258708 38.0 37.0 38.0 34.0 38.0 66-67 35.87812223473574 38.0 37.0 38.0 32.0 38.0 68-69 35.73169395060735 38.0 37.0 38.0 31.0 38.0 70-71 34.8625764218486 38.0 36.0 38.0 26.5 38.0 72-73 33.51107614029442 38.0 34.0 38.0 20.0 38.0 74-75 33.13155619016974 38.0 34.0 38.0 11.0 38.0 76 32.312856970477036 37.0 31.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2467533521019405 1101 2 0.06740537550725634 1101 3 0.18444992798042392 1101 4 0.1658793137212271 1101 5 0.1240374413406613 1101 6 0.05437311486441132 1101 7 0.163877582589258 1101 8 0.1959461602966357 1101 9 0.3664494014553341 1101 10-11 0.2911696073489196 1101 12-13 0.3080488570040245 1101 14-15 0.2510641945428702 1101 16-17 0.24401369867653244 1101 18-19 0.19802052363429823 1101 20-21 0.26235066224712966 1101 22-23 0.27046150507295863 1101 24-25 0.22276986511683106 1101 26-27 0.34833501601453065 1101 28-29 -0.06582166153218338 1101 30-31 0.01844055261515365 1101 32-33 0.13990272080554433 1101 34-35 0.2508724714687034 1101 36-37 0.3417140894672528 1101 38-39 0.4039204553152089 1101 40-41 0.5757902497437968 1101 42-43 0.457830027453177 1101 44-45 0.4874800096795795 1101 46-47 0.3603250040882813 1101 48-49 0.430906252826162 1101 50-51 0.4262401036129546 1101 52-53 0.36313965887372035 1101 54-55 -0.033542329520855674 1101 56-57 0.26446084249214863 1101 58-59 0.46610726368592736 1101 60-61 0.2631203090064318 1101 62-63 0.2534028136357023 1101 64-65 0.1036770953523174 1101 66-67 -0.236215880838472 1101 68-69 -1.043359340939972 1101 70-71 -0.27599837582295805 1101 72-73 -0.08811653225428984 1101 74-75 0.3323599941582458 1101 76 -0.4044138968255311 1102 1 0.10636933503451473 1102 2 0.21458969481033563 1102 3 0.0014195710756723656 1102 4 0.147322933437664 1102 5 0.24195379317852428 1102 6 0.20360196948017517 1102 7 0.28486073739613715 1102 8 0.167767130117511 1102 9 0.03905707290740423 1102 10-11 0.19029845752003638 1102 12-13 0.33765017261804786 1102 14-15 0.16123798004444723 1102 16-17 0.32680626723513484 1102 18-19 0.33310430513569855 1102 20-21 0.33612060757803164 1102 22-23 0.22434834448804253 1102 24-25 0.24308187898411404 1102 26-27 0.3058997966714472 1102 28-29 0.3824093709581433 1102 30-31 0.44127045616642846 1102 32-33 0.5388426736923364 1102 34-35 0.35386593784497933 1102 36-37 0.6132039334603192 1102 38-39 0.9574228331986632 1102 40-41 0.41011621978690727 1102 42-43 0.5362112888425727 1102 44-45 0.6345976593528988 1102 46-47 0.6778391158265862 1102 48-49 0.6019916343986722 1102 50-51 0.5793357967548687 1102 52-53 0.37617357149206043 1102 54-55 0.5809627595942217 1102 56-57 0.509582847914615 1102 58-59 0.4076491099902029 1102 60-61 0.6050355052373746 1102 62-63 0.5025027736339283 1102 64-65 0.5202511653109809 1102 66-67 0.47440459118250544 1102 68-69 0.666049966140335 1102 70-71 0.4818575288839142 1102 72-73 0.6621279230548325 1102 74-75 0.49671174312486954 1102 76 0.7704605420384212 1103 1 0.09054177671958286 1103 2 0.18279247993592662 1103 3 -0.03483734255045334 1103 4 0.07031661553109814 1103 5 0.2643506626448868 1103 6 0.2503413612996894 1103 7 0.1424892270011071 1103 8 0.17639140049767832 1103 9 0.08942543527978586 1103 10-11 0.1383178266038314 1103 12-13 0.09705051802608722 1103 14-15 0.17438753298151255 1103 16-17 0.15943639643523255 1103 18-19 0.2503211470568658 1103 20-21 0.24165260154431678 1103 22-23 0.3174554865644339 1103 24-25 0.26245642809545444 1103 26-27 0.16407481798618306 1103 28-29 0.10733449611390711 1103 30-31 -0.020253711465777258 1103 32-33 0.3525081525634235 1103 34-35 0.11002535021408733 1103 36-37 0.4312744579121457 1103 38-39 0.33289558312761613 1103 40-41 0.33788658902373214 1103 42-43 0.4107713308162104 1103 44-45 0.19252455527139745 1103 46-47 0.0992533212787805 1103 48-49 0.31981731264452407 1103 50-51 0.06132757691795376 1103 52-53 0.28421414600619244 1103 54-55 0.45333667472877437 1103 56-57 -0.17302432695367287 1103 58-59 0.38027880196493413 1103 60-61 0.33319063279787287 1103 62-63 0.3094623084261343 1103 64-65 0.3482248443438465 1103 66-67 -0.11514462946327342 1103 68-69 0.010370408612409676 1103 70-71 0.40217320559947467 1103 72-73 0.35702659607584764 1103 74-75 0.03155860493979645 1103 76 0.377700994129448 1104 1 0.10471327297577204 1104 2 0.09892683418102877 1104 3 0.1578738550467378 1104 4 0.07105691757433164 1104 5 0.22563286578376562 1104 6 0.08617409534089404 1104 7 0.0792039780481062 1104 8 0.10697222032931819 1104 9 0.12406099515964542 1104 10-11 0.15184949037979578 1104 12-13 0.08651707752522242 1104 14-15 0.18824704480519472 1104 16-17 0.05324006833336625 1104 18-19 0.1083047758059692 1104 20-21 -0.1068868882818137 1104 22-23 -0.029497641740491076 1104 24-25 0.13160275408216648 1104 26-27 0.26462898694425974 1104 28-29 -0.1538387768671896 1104 30-31 0.0711841666170514 1104 32-33 0.08121389307698479 1104 34-35 -0.060127786551426254 1104 36-37 0.21961681587990967 1104 38-39 -0.2102534502494322 1104 40-41 0.007791195817588914 1104 42-43 -0.0033271928099978254 1104 44-45 -0.050706968904755456 1104 46-47 -6.451515838605815E-4 1104 48-49 0.1606946763231889 1104 50-51 -0.05731111338197792 1104 52-53 -0.008860857166524738 1104 54-55 -0.40183380625603604 1104 56-57 -0.12375193810473917 1104 58-59 -0.03752651974917853 1104 60-61 -0.10404232967938754 1104 62-63 -0.031816933504572376 1104 64-65 0.14845433797724183 1104 66-67 0.031974538014196696 1104 68-69 0.1592187101480107 1104 70-71 0.10789256767063904 1104 72-73 0.5608793394236997 1104 74-75 0.34140144939176054 1104 76 0.7265313169011378 1105 1 -0.07640995162571329 1105 2 0.061203687762230174 1105 3 0.1931212163810585 1105 4 0.16455038063300975 1105 5 0.23893478125959433 1105 6 0.23531842926783497 1105 7 0.011836562854604438 1105 8 0.17655481165103737 1105 9 0.4662079786109743 1105 10-11 0.2709532127443879 1105 12-13 0.08017559292286336 1105 14-15 0.41803356263518765 1105 16-17 0.13825831096030328 1105 18-19 0.32533231348568137 1105 20-21 0.37811180867340255 1105 22-23 0.29034012918423713 1105 24-25 0.14514356110123572 1105 26-27 0.2467989908533923 1105 28-29 0.3114513677707862 1105 30-31 0.19277996212588988 1105 32-33 0.18518057856540793 1105 34-35 0.30988263780682246 1105 36-37 0.2457374343918346 1105 38-39 0.20018393844883065 1105 40-41 0.4277028059465877 1105 42-43 -0.0028114042507354498 1105 44-45 0.10831878794709127 1105 46-47 0.1348715056719243 1105 48-49 -0.1464764134565968 1105 50-51 -0.1583188013461836 1105 52-53 0.16761484104830515 1105 54-55 0.04243388257687286 1105 56-57 0.32728615423769725 1105 58-59 0.49557081769598454 1105 60-61 0.3744682901355745 1105 62-63 0.40615813490527586 1105 64-65 0.26539717774616634 1105 66-67 -0.1786952270033879 1105 68-69 0.2971188534829281 1105 70-71 -0.4151930423714987 1105 72-73 -0.34796724877071483 1105 74-75 -0.20239714331987813 1105 76 0.6033935850677565 1106 1 0.15881089948718596 1106 2 0.08320807018640863 1106 3 0.08572875876371455 1106 4 0.23281240346774723 1106 5 0.20133819493737093 1106 6 0.15234068736955209 1106 7 0.1575291012008151 1106 8 0.2366632189858322 1106 9 0.15717787827652785 1106 10-11 0.19400130408333638 1106 12-13 0.016543947207267706 1106 14-15 0.2138068298477407 1106 16-17 0.08717423761236631 1106 18-19 0.17996845695240182 1106 20-21 0.24148973509480243 1106 22-23 0.2800298996836048 1106 24-25 0.138835176831833 1106 26-27 2.7609845344045425E-4 1106 28-29 0.3832077972483603 1106 30-31 0.34778515183872827 1106 32-33 0.1593934721186301 1106 34-35 0.25285922643558223 1106 36-37 0.28210002232862763 1106 38-39 0.19299330299732276 1106 40-41 0.4736778953249754 1106 42-43 0.22877088152944935 1106 44-45 0.07376683315525412 1106 46-47 0.18397195544704203 1106 48-49 0.4841832567083202 1106 50-51 0.4240592404021726 1106 52-53 0.37918213432473635 1106 54-55 0.4246216348545744 1106 56-57 0.27183695576002265 1106 58-59 0.3479638519480872 1106 60-61 0.3533692242838882 1106 62-63 0.4506282075612589 1106 64-65 0.4516675041214455 1106 66-67 0.3096279191158544 1106 68-69 0.6884059022662399 1106 70-71 0.674081551100997 1106 72-73 0.7729818920434965 1106 74-75 0.8821202133863792 1106 76 1.2174404078102299 1107 1 0.017235533324118535 1107 2 -0.09933142929462235 1107 3 0.0493085073509576 1107 4 0.08428282210458349 1107 5 -0.03865719198454798 1107 6 -0.025551135949555714 1107 7 0.015181044459950499 1107 8 -0.09434819838240571 1107 9 -0.0923204494826706 1107 10-11 -0.07185615180410565 1107 12-13 -0.14892139704368645 1107 14-15 0.1237191813642795 1107 16-17 0.01952921397033691 1107 18-19 -0.2733298938721802 1107 20-21 0.20419876519514446 1107 22-23 0.22010601547186326 1107 24-25 0.16353820993067103 1107 26-27 -0.0057093703506154725 1107 28-29 0.2512506988744647 1107 30-31 0.003816751423549647 1107 32-33 -0.14090637795633398 1107 34-35 -0.027910004333641325 1107 36-37 -0.10710537497271844 1107 38-39 0.09650500868294642 1107 40-41 0.0447596621338775 1107 42-43 0.057389264645586024 1107 44-45 0.007984339786553107 1107 46-47 -0.23803819332472642 1107 48-49 0.10770418855009467 1107 50-51 0.0540557805936146 1107 52-53 -0.06482663052360493 1107 54-55 0.08256766184108244 1107 56-57 -0.3315767220164787 1107 58-59 0.0474102825789231 1107 60-61 -0.16232100752328193 1107 62-63 0.016526027881866412 1107 64-65 0.35904266269599816 1107 66-67 0.4350171475785487 1107 68-69 0.5395937698708906 1107 70-71 0.08815143923384738 1107 72-73 0.2272835873496888 1107 74-75 0.533388809857378 1107 76 -0.17921511058441553 1108 1 0.17661697902581608 1108 2 0.0699436222709906 1108 3 -0.007314615019780035 1108 4 0.03003696192023142 1108 5 -0.11717676361122642 1108 6 -0.08321139721532944 1108 7 0.15585903031291082 1108 8 0.25172990167236264 1108 9 0.18226498629046972 1108 10-11 0.11791695949604275 1108 12-13 0.0687787183234505 1108 14-15 0.13823912589483456 1108 16-17 0.11821472782022369 1108 18-19 0.26014988191936794 1108 20-21 0.20333642499028315 1108 22-23 0.23986409408465903 1108 24-25 0.15290462294501594 1108 26-27 0.019776278676921777 1108 28-29 0.17816153988923134 1108 30-31 0.313344445362695 1108 32-33 0.1649563701121437 1108 34-35 0.2568661950767037 1108 36-37 0.2076196666876413 1108 38-39 0.06041490578477493 1108 40-41 0.4838364919848388 1108 42-43 0.2551856358247804 1108 44-45 0.06989803584659882 1108 46-47 0.4577013799333969 1108 48-49 0.36927877515118723 1108 50-51 0.32026253248435665 1108 52-53 0.23488320365070336 1108 54-55 -0.19352686929056517 1108 56-57 0.25722683921550527 1108 58-59 0.06557769311113049 1108 60-61 0.5016751235990355 1108 62-63 0.5229819261723421 1108 64-65 0.4669925071251697 1108 66-67 0.6087087793825816 1108 68-69 0.5072152258274514 1108 70-71 0.7132140754311678 1108 72-73 0.241232522825662 1108 74-75 0.7140257762843802 1108 76 1.2355588747091844 1109 1 0.028316360279923458 1109 2 0.0034191390292050983 1109 3 0.1173645422954337 1109 4 0.05633676228477924 1109 5 0.18980303724931247 1109 6 -0.17082779956135852 1109 7 -0.23904716582581642 1109 8 -0.22962712338746627 1109 9 0.13440207756478628 1109 10-11 -0.05513969481223313 1109 12-13 0.02178998966646617 1109 14-15 0.11571503944400519 1109 16-17 0.11466840867630168 1109 18-19 0.39717915765187684 1109 20-21 0.1067922261980101 1109 22-23 0.016390189289687385 1109 24-25 0.21931130887257666 1109 26-27 0.2290649068961983 1109 28-29 0.06810164925473572 1109 30-31 0.07321772025202478 1109 32-33 0.08035309906672694 1109 34-35 0.0017916107772251166 1109 36-37 0.06990220664572888 1109 38-39 -0.24170420589947383 1109 40-41 -0.020106618112258445 1109 42-43 -0.04616469338771623 1109 44-45 0.2149375289461517 1109 46-47 0.2397763486241189 1109 48-49 -0.04760591987776053 1109 50-51 -0.11106163332465968 1109 52-53 0.15946192325589692 1109 54-55 0.3046007733991871 1109 56-57 0.013547595102266996 1109 58-59 0.29410448270032674 1109 60-61 0.3398154140004195 1109 62-63 -0.13016698203255572 1109 64-65 0.021755858520755567 1109 66-67 0.11392906036017791 1109 68-69 0.1581383847794271 1109 70-71 0.38117444593471816 1109 72-73 -0.19440674459904272 1109 74-75 0.01119740077133713 1109 76 0.4971557103013353 1110 1 0.09821976648226638 1110 2 0.1700307302849282 1110 3 -0.10835991118702992 1110 4 0.03047250198991236 1110 5 0.12855494370584353 1110 6 -0.3667479825811881 1110 7 -0.04222459338046747 1110 8 -0.07971609135899627 1110 9 0.00717787827652927 1110 10-11 -0.04571544468513622 1110 12-13 0.15989370090184707 1110 14-15 0.014545746103891588 1110 16-17 -0.050818373225077096 1110 18-19 0.12713594463713918 1110 20-21 0.04684687795194975 1110 22-23 -0.16553660277945426 1110 24-25 -0.06547516799575703 1110 26-27 -0.008098285783006531 1110 28-29 0.06596641793801439 1110 30-31 -0.302030119097239 1110 32-33 -0.1079094835463934 1110 34-35 -0.13106934499298717 1110 36-37 0.18536356912666463 1110 38-39 -0.021292411288392543 1110 40-41 0.15957691010330421 1110 42-43 -0.04228823177103891 1110 44-45 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-0.019507828501417634 2116 3 0.06357434924280625 2116 4 0.0638163705107786 2116 5 0.14015418517234224 2116 6 9.827380256410834E-4 2116 7 0.19930511584835386 2116 8 0.25546089979144426 2116 9 -0.02600796243143577 2116 10-11 0.025096818273141253 2116 12-13 0.025317215440402663 2116 14-15 -0.06351999346627224 2116 16-17 -0.41186451734643725 2116 18-19 -0.24293358760053962 2116 20-21 -0.13948371623262545 2116 22-23 0.05987426953407038 2116 24-25 -0.1931300352523877 2116 26-27 0.12812168893253784 2116 28-29 -0.23447605993810328 2116 30-31 -0.1803961115119037 2116 32-33 -0.37869014094209064 2116 34-35 -0.14669829568831005 2116 36-37 -0.2836155712020414 2116 38-39 -0.0012544846132911402 2116 40-41 -0.1921139874946718 2116 42-43 -0.2820164239328591 2116 44-45 -0.06570524496497399 2116 46-47 0.06946173268231348 2116 48-49 -0.07048564777748823 2116 50-51 -0.005937708026266364 2116 52-53 -0.0735947957942713 2116 54-55 -0.03209182257599963 2116 56-57 0.047119226129254344 2116 58-59 -0.30376028598294624 2116 60-61 -0.3566857040041782 2116 62-63 0.08483632474160885 2116 64-65 0.31227171529019415 2116 66-67 0.20198373235967892 2116 68-69 -0.1443527184234199 2116 70-71 0.19072787395726465 2116 72-73 -0.6638505766778948 2116 74-75 -0.519146188810744 2116 76 -0.4391967603313631 2117 1 -0.07459129342556992 2117 2 0.04802612198999867 2117 3 -0.01158571763863847 2117 4 -0.09798371920824422 2117 5 -0.057044134635169996 2117 6 0.11078657963079763 2117 7 -0.18807712794267673 2117 8 -0.8360524968889536 2117 9 -0.29812166089397607 2117 10-11 -0.21927535251924013 2117 12-13 -0.5689081424161273 2117 14-15 -0.16517775620025077 2117 16-17 -0.2241478663126273 2117 18-19 -0.17274884798959533 2117 20-21 -0.40113699301578976 2117 22-23 -0.025674851627378814 2117 24-25 -0.38609728781142394 2117 26-27 -0.1163932166585866 2117 28-29 -1.3017755175458632 2117 30-31 -0.22835500388986674 2117 32-33 -0.8420684697215037 2117 34-35 -0.34091957540773166 2117 36-37 -0.30075394239407416 2117 38-39 -0.4316610748828609 2117 40-41 -0.7715866843667456 2117 42-43 -0.3320347755498432 2117 44-45 -0.3863793610287587 2117 46-47 -0.10956848631364835 2117 48-49 -0.29594545773629477 2117 50-51 -0.2512784363815541 2117 52-53 -0.2801218542339754 2117 54-55 -0.26636040868586974 2117 56-57 -0.39344192553666346 2117 58-59 -0.17933632284930923 2117 60-61 -0.7654373862357389 2117 62-63 -0.45200964401986 2117 64-65 -0.4944551718677488 2117 66-67 -0.26557468695438047 2117 68-69 -0.25956009169530603 2117 70-71 -0.3146042903100934 2117 72-73 -0.3540243053186529 2117 74-75 -0.5552784835784976 2117 76 -1.1364766428810142 2118 1 -0.451373127670216 2118 2 -0.3058575006551152 2118 3 -0.39481438935135316 2118 4 -0.4633283862410238 2118 5 -0.4120261073674314 2118 6 -0.13182940375069307 2118 7 -0.2716472432694417 2118 8 -0.17362800845744175 2118 9 -0.17546460877010617 2118 10-11 -0.14988827962222473 2118 12-13 -0.3467310215259829 2118 14-15 -0.5568828253246778 2118 16-17 -0.35473214080465 2118 18-19 -0.7083377785308826 2118 20-21 -0.3105828037652998 2118 22-23 -0.45084378264621705 2118 24-25 -0.5974699866486048 2118 26-27 -0.598401764687523 2118 28-29 -0.1889558618547369 2118 30-31 -0.196765500296344 2118 32-33 -0.41078698169591377 2118 34-35 -0.7194205663105251 2118 36-37 -0.46135182687630305 2118 38-39 -0.22902742239127605 2118 40-41 -0.8418572127686446 2118 42-43 -0.7377915626370637 2118 44-45 -0.3652719376147999 2118 46-47 -0.7290618126430104 2118 48-49 -0.8246697002328958 2118 50-51 -0.6607361857189673 2118 52-53 -0.9828868311925021 2118 54-55 -0.825181831287999 2118 56-57 -0.9038643127766974 2118 58-59 -1.3218950010088548 2118 60-61 -0.7102094785926525 2118 62-63 -0.4366685585634542 2118 64-65 -0.6163242771899746 2118 66-67 -1.0977778339661484 2118 68-69 -0.5847672261954386 2118 70-71 -0.6559523956561932 2118 72-73 -0.8692818206597437 2118 74-75 -0.7285551484145785 2118 76 -1.317274618096505 2119 1 -0.23485476767923075 2119 2 0.05326054394952706 2119 3 -0.2015276130503807 2119 4 -0.09297470297902777 2119 5 -0.36725250971030476 2119 6 0.09986692425110988 2119 7 0.08541515817232437 2119 8 -0.4438465261416056 2119 9 -0.3623873391147754 2119 10-11 -0.4194732086602997 2119 12-13 -0.38366219971927507 2119 14-15 -0.9590567383681616 2119 16-17 -0.6658804850263138 2119 18-19 -0.49072119822000815 2119 20-21 -0.4101406996878012 2119 22-23 -0.41367324874218525 2119 24-25 -0.20080125495227463 2119 26-27 -0.6396976646649932 2119 28-29 -0.6519683646706866 2119 30-31 -0.5014090010848165 2119 32-33 -0.7957976823041548 2119 34-35 -0.12051033878180561 2119 36-37 -0.2293879836683672 2119 38-39 -0.5629849578722386 2119 40-41 -0.30160321412029845 2119 42-43 -0.49842177214370764 2119 44-45 -0.7825674996783292 2119 46-47 -0.5553458856324198 2119 48-49 -0.5866938047609409 2119 50-51 -0.5500412093729423 2119 52-53 -0.20571291814901826 2119 54-55 -0.2625410338110896 2119 56-57 -0.1493024745248377 2119 58-59 -0.44234724250468105 2119 60-61 -0.45956181019887055 2119 62-63 -0.2746529018840178 2119 64-65 -0.9256563339595161 2119 66-67 -0.6393975681405095 2119 68-69 -1.0872875010321152 2119 70-71 -0.8721328417026015 2119 72-73 -0.8237197571319186 2119 74-75 -0.7542366082028238 2119 76 -2.4325595921897687 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 2.0 21 2.0 22 6.0 23 12.0 24 32.0 25 69.0 26 155.0 27 253.0 28 479.0 29 792.0 30 1180.0 31 1788.0 32 2867.0 33 4611.0 34 7361.0 35 11539.0 36 15825.0 37 52474.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.009773952216236 14.702155900571153 12.246601238838387 37.04146890837423 2 19.876719491593597 16.975705896548952 35.883074571635426 27.26450004022203 3 19.53885447671145 16.13204890998311 31.885005228863328 32.44409138444212 4 16.04054380178586 23.368996862682003 28.74467058161049 31.84578875392165 5 17.6564636795109 27.494771136674444 37.031413401978924 17.817351781835733 6 31.31284691497064 34.454187112863 15.27129756254525 18.961668409621108 7 28.782881505912638 22.584667363848443 19.724881345024535 28.90756978521438 8 24.551524414769528 35.20231678867348 14.375351942723835 25.870806853833162 9 22.16635829780388 15.230069986324512 14.845949642023973 47.75762207384764 10-11 27.8944775158877 25.06586356688923 21.338287346150754 25.70137157107232 12-13 26.350454508889065 16.231095647976833 24.357453141340198 33.060996701793904 14-15 23.46201029683855 15.552851741613708 18.50263454267557 42.48250341887218 16-17 30.256515968144154 23.175931139892207 16.6061660365216 29.96138685544204 18-19 32.794023006998636 20.916961628187593 17.062686026868313 29.22632933794546 20-21 26.58927278577749 18.657992116482987 17.00436408977556 37.74837100796396 22-23 24.692301504303757 23.092470436811197 18.113989220497146 34.10123883838791 24-25 30.51695358378248 22.992418148177943 18.723855683372214 27.766772584667365 26-27 31.984051966857052 24.579679832676373 17.881707022765667 25.554561177700908 28-29 25.655619016973695 19.92800257420964 23.529382189687073 30.886996219129596 30-31 24.195559488375835 18.781172069825438 30.685383315903792 26.33788512589494 32-33 27.926655136352668 17.61925830584828 23.184981095647977 31.269105462151074 34-35 26.949762690049074 17.537808704046338 24.77928163462312 30.73314697128147 36-37 25.151335371249296 26.928143351299173 20.391561419033064 27.52895985841847 38-39 23.33581369157751 22.933593435765427 25.29060413482423 28.439988737832838 40-41 24.07187675971362 16.98827527954308 26.346432306330946 32.593415654412354 42-43 29.801504303756737 16.48198053253962 25.351439948515807 28.365075215187836 44-45 23.30464162175207 22.665614190330626 28.503841203443002 25.525902984474296 46-47 27.56515968144156 18.60922291046577 25.41076743624809 28.414849971844582 48-49 27.344944091384445 17.075255409862443 21.57056954388223 34.00923095487089 50-51 24.59979084546698 17.897293057678386 26.067894779181078 31.435021317673556 52-53 20.682668329177055 17.73338830343496 34.058502936207866 27.525440431180115 54-55 21.6012388383879 18.600675730029764 28.834667363848443 30.96341806773389 56-57 26.165935966535276 19.889288874587724 22.8461105301263 31.098664628750704 58-59 22.229205212774517 20.276425870806854 29.578774837100795 27.915594079317835 60-61 26.394195961708633 24.623924060815703 23.29257501407771 25.68930496339796 62-63 21.373984393854077 34.03235861958008 19.070770654010136 25.522886332555707 64-65 20.32217842490548 35.62867025983428 18.24370525299654 25.805446062263698 66-67 20.567030005631086 35.20935564315019 18.021478561660366 26.202135789558362 68-69 19.894819403105142 35.323988416056636 18.732402863808222 26.048789317030007 70-71 19.926494248250343 33.385286783042396 19.96571072319202 26.722508245515243 72-73 20.171345828975948 29.72005470195479 21.07231920199501 29.03628026707425 74-75 20.284470275923095 28.58227415332636 21.93608720135146 29.197168369399083 76 20.212573405196686 28.333400370042632 21.966253720537367 29.487772504223315 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 1.5 17 2.0 18 5.0 19 4.0 20 4.5 21 9.0 22 19.5 23 37.5 24 49.0 25 53.0 26 75.0 27 110.5 28 170.5 29 217.0 30 250.0 31 355.5 32 538.5 33 649.0 34 716.0 35 914.0 36 1082.5 37 1120.0 38 1472.5 39 3670.5 40 6632.0 41 7342.5 42 6937.0 43 6084.5 44 4704.5 45 3866.5 46 3556.0 47 3438.5 48 3123.0 49 2716.0 50 2507.0 51 2349.0 52 1960.0 53 1689.5 54 1650.0 55 1637.0 56 1599.5 57 1583.5 58 1592.0 59 1713.0 60 1925.0 61 2186.0 62 2356.0 63 2533.0 64 2774.0 65 2939.0 66 3114.5 67 3189.0 68 3309.5 69 3462.0 70 3372.0 71 3250.0 72 3211.5 73 2987.0 74 2527.0 75 2253.0 76 1989.0 77 1415.0 78 912.0 79 719.0 80 527.0 81 231.5 82 81.0 83 34.0 84 25.5 85 8.5 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 99448.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.19909902662698 #Duplication Level Percentage of deduplicated Percentage of total 1 66.10312086855494 33.18317110449682 2 20.459917471255157 20.541388464323063 3 7.018949561315653 10.570348322741534 4 2.6861904571130966 5.3937736304400286 5 1.2359280477544972 3.102123722950688 6 0.7671968270501983 2.310755369640415 7 0.4627218460798846 1.6259753841203441 8 0.348543728216017 1.3997264902260478 9 0.21233123672929768 0.9592953101118172 >10 0.6630343335603541 4.719049151315261 >50 0.018028123873242256 0.6184136433110772 >100 0.014021874123632866 1.2036441155176574 >500 0.002003124874804695 0.5781916177298688 >1k 0.006009374624414086 5.9779985520070795 >5k 0.002003124874804695 7.816145121068296 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT 7773 7.816145121068296 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG 2116 2.1277451532459173 No Hit CACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTTCTG 2108 2.119700748129676 RNA PCR Primer, Index 15 (96% over 25bp) CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC 1721 1.730552650631486 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC 575 0.5781916177298688 No Hit ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT 333 0.33484836296355885 RNA PCR Primer, Index 48 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT 197 0.19809347598745072 No Hit CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG 145 0.14580484273187996 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC 142 0.14278819081328936 No Hit CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAACTCGTATGCCGT 137 0.13776043761563833 No Hit CTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG 126 0.12669938058080607 No Hit CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT 117 0.11764942482503418 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0010055506395302068 0.0 3 0.0 0.0 0.0 0.0010055506395302068 0.0 4 0.0 0.0 0.0 0.01206660767436248 0.0 5 0.0 0.0 0.0 0.014077708953422894 0.0 6 0.0 0.0 0.0 0.014077708953422894 0.0 7 0.0 0.0 0.0 0.014077708953422894 0.0 8 0.0 0.0 0.0 0.014077708953422894 0.0 9 0.0 0.0 0.0 0.017094360872013513 0.0 10 0.0 0.0 0.0 0.021116563430134343 0.0 11 0.0 0.0 0.0 0.021116563430134343 0.0 12 0.0 0.0 0.0 0.021116563430134343 0.0 13 0.0 0.0 0.0 0.021116563430134343 0.0 14 0.0 0.0 0.0 0.021116563430134343 0.0 15 0.0 0.0 0.0 0.022122114069664547 0.0 16 0.0 0.0 0.0 0.023127664709194754 0.0 17 0.0 0.0 0.0 0.02513876598825517 0.0 18 0.0 0.0 0.0 0.02714986726731558 0.0 19 0.0 0.0 0.0 0.03519427238355723 0.0 20 0.0 0.0 0.0 0.0452497787788593 0.0 21 0.0 0.0 0.0 0.04826643069744992 0.0 22 0.0 0.0 0.0 0.05731638645322178 0.0 23 0.0 0.0 0.0 0.06737189284852385 0.0 24 0.0 0.0 0.0 0.07139409540664468 0.0 25 0.0 0.0 0.0 0.08245515244147696 0.0 26 0.0 0.0 0.0 0.09854396267396026 0.0 27 0.0 0.0 0.0 0.17194915935966534 0.0 28 0.0 0.0 0.0 0.2584265143592631 0.0 29 0.0 0.0 0.0 0.38512589494006916 0.0 30 0.0 0.0 0.0 0.4796476550559086 0.0 31 0.0 0.0 0.0 0.6194191939506074 0.0 32 0.0 0.0 0.0 0.768240688601078 0.0 33 0.0 0.0 0.0 0.9472287024374547 0.0 34 0.0 0.0 0.0 1.1925830584828252 0.0 35 0.0 0.0 0.0 1.635025339876116 0.0 36 0.0 0.0 0.0 2.2112058563269246 0.0 37 0.0 0.0 0.0 2.8819081328935727 0.0 38 0.0 0.0 0.0 3.5656825677741133 0.0 39 0.0 0.0 0.0 4.265545812887137 0.0 40 0.0 0.0 0.0 4.95937575416298 0.0 41 0.0 0.0 0.0 5.670300056310836 0.0 42 0.0 0.0 0.0 6.440551846190974 0.0 43 0.0 0.0 0.0 7.2490145603732605 0.0 44 0.0 0.0 0.0 8.006194191939507 0.0 45 0.0 0.0 0.0 8.66482986083179 0.0 46 0.0 0.0 0.0 9.38580966937495 0.0 47 0.0 0.0 0.0 10.010256616523208 0.0 48 0.0 0.0 0.0 10.646770171345828 0.0 49 0.0 0.0 0.0 11.174684257099187 0.0 50 0.0 0.0 0.0 11.698576140294426 0.0 51 0.0 0.0 0.0 12.265706700989462 0.0 52 0.0 0.0 0.0 12.98065320569544 0.0 53 0.0 0.0 0.0 13.739843938540744 0.0 54 0.0 0.0 0.0 14.434679430456118 0.0 55 0.0 0.0 0.0 15.12549271981337 0.0 56 0.0 0.0 0.0 15.66446786260156 0.0 57 0.0 0.0 0.0 16.225565119459414 0.0 58 0.0 0.0 0.0 16.78062907248009 0.0 59 0.0 0.0 0.0 17.280387740326603 0.0 60 0.0 0.0 0.0 17.814335129917144 0.0 61 0.0 0.0 0.0 18.354315823344862 0.0 62 0.0 0.0 0.0 18.916418630842248 0.0 63 0.0 0.0 0.0 19.50164910304883 0.0 64 0.0 0.0 0.0 20.106990588046013 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTTTAT 90 0.0 66.111115 1 GTCAAGC 30 8.324141E-6 58.333336 33 CCTTATA 25 2.3803893E-4 55.999996 2 CTTTATA 100 0.0 55.999996 2 GTCGCTC 20 0.0069334623 52.5 46 GGAGCTT 20 0.0069334623 52.5 70 AACTAAA 20 0.0069334623 52.5 70 TCCTTAT 20 0.0069334623 52.5 1 TTTATAC 115 0.0 51.73913 3 CGTCAAG 35 2.0736094E-5 50.000004 32 TCAAGCT 40 4.5644338E-5 43.75 34 GACAGGG 35 0.0012502433 40.000004 41 GCTCGTA 45 9.1413705E-5 38.88889 38 CTTAATA 40 0.0024088526 35.0 2 ATCGTAT 50 1.6992886E-4 35.0 39 GTCTTCT 1745 0.0 33.295128 49 CCGTCTT 1750 0.0 33.2 47 TCTTCTG 1755 0.0 33.105415 50 TCTGCTT 1745 0.0 33.094555 53 ATGCCGT 1770 0.0 33.0226 44 >>END_MODULE