FastQCFastQC Report
Wed 25 May 2016
SRR1780353_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780353_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380291
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTAAT450.00138891924.4671439
GTCTTAG654.96289E-420.2837771
GAGGGTA550.004433706420.03441220
GAAGTTG550.004437092720.03176919
GCAACTC550.004484724319.99485425
CTATACA550.00450869719.9764481
AAGTATG600.007413976418.32861727
TCCAGAT600.007453474718.3117452
ATCCAGA600.007453474718.3117451
ACGAGAG800.001974430216.51314424
TATAAGA953.368877E-416.1914372
CTTTACA850.002998369315.5111251
ATTTTAC850.003000998915.5090853
TAAGAGA850.003000998915.5090854
TGGTAGG850.003000998915.5090854
GACGAAA1351.9488747E-514.66675523
GTGTATG1057.206956E-414.6493961
GTATAAG1057.206956E-414.6493961
ATAAGAG900.004389751214.6474683
AGTACCG900.004389751214.6474685
Produced by FastQC (version 0.11.5)