FastQCFastQC Report
Wed 25 May 2016
SRR1780353_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780353_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380291
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGT189604.985655721539558No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG51491.3539631492725308No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC48761.2821760178389707No Hit
CACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTTCTG31590.8306796637311953RNA PCR Primer, Index 13 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC10490.2758413951421412No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTT5710.15014817600206157TruSeq Adapter, Index 8 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCTG4100.037.0215144
CTTTATA1750.035.2065282
GCTTATA250.002348634935.2065243
TTTTTGC250.00235166835.19726630
GGCTTTT250.00235166835.19726627
TAACCGG250.00235166835.19726622
CGGCTTT250.00235166835.19726626
GTCTTCT4450.034.10970743
TCTTTAT1750.034.0297131
ATGCCGT24800.032.64262444
CCGTCTT4750.031.49228741
AAAGCGA353.2152122E-431.42612825
CAAAGCG353.2152122E-431.42612824
AATATCG651.7416824E-830.45917136
ATATCGT651.7416824E-830.45917137
TATCGTA651.7416824E-830.45917138
CGTCTTC4950.030.21987542
TTTATAC2150.029.6799183
TCTGTAC300.005668862729.4083961
TTGTACC300.00574230529.33105716
Produced by FastQC (version 0.11.5)