FastQCFastQC Report
Wed 25 May 2016
SRR1780352_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780352_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152805
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATTA308.345514E-658.33333237
GATCGAA200.00694056352.50000430
CTAAGGG200.00694056352.50000436
CTTATTG200.00694056352.50000440
CTTATAA200.00694056352.5000041
AGCTAGA200.00694056352.5000041
AGGACCG305.863326E-446.6666645
GTAGGTA305.863326E-446.66666447
GTAGATT404.5760342E-543.75000436
GGTGCTA459.1644935E-538.8888958
AGCCCGG400.00241289635.00000430
AAAGGAG400.00241289635.0000045
GTGCTAA501.7035654E-435.059
AGTGTGA552.9814086E-431.8181819
GAGGACC450.004296892331.1111134
CGGGCTA450.004296892331.11111341
GTTAGAT604.964334E-429.1666663
TAACCGT500.007191126227.9999984
CATAGAC500.007191126227.9999982
TCTCGTT500.007191126227.99999843