##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780352_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 152805 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.962547037073396 34.0 32.0 34.0 21.0 34.0 2 30.981577827950655 34.0 32.0 34.0 21.0 34.0 3 31.000477733058474 34.0 33.0 34.0 21.0 34.0 4 30.82096135597657 34.0 32.0 34.0 21.0 34.0 5 30.90516017146036 34.0 32.0 34.0 21.0 34.0 6 33.75549229410032 37.0 34.0 38.0 21.0 38.0 7 34.10549393017244 38.0 34.0 38.0 23.0 38.0 8 33.95581950852394 38.0 34.0 38.0 23.0 38.0 9 34.4438663656294 38.0 36.0 38.0 24.0 38.0 10-11 34.246804751153434 38.0 34.5 38.0 23.5 38.0 12-13 34.31412257452308 38.0 35.0 38.0 23.5 38.0 14-15 34.364134027027916 38.0 35.0 38.0 24.0 38.0 16-17 34.4560878243513 38.0 36.0 38.0 24.0 38.0 18-19 34.44712542128857 38.0 35.5 38.0 23.5 38.0 20-21 34.469068420535976 38.0 36.0 38.0 24.0 38.0 22-23 34.45256045286476 38.0 36.0 38.0 24.0 38.0 24-25 34.547845293020515 38.0 36.0 38.0 24.5 38.0 26-27 34.57386538398613 38.0 36.0 38.0 24.0 38.0 28-29 34.723372926278586 38.0 36.0 38.0 26.0 38.0 30-31 34.72400445011616 38.0 36.0 38.0 25.5 38.0 32-33 34.80994077418933 38.0 36.0 38.0 26.0 38.0 34-35 34.74312686103204 38.0 36.0 38.0 25.5 38.0 36-37 34.68772291482608 38.0 36.0 38.0 25.0 38.0 38-39 34.70829488563856 38.0 36.0 38.0 25.5 38.0 40-41 34.72983541114492 38.0 36.0 38.0 25.5 38.0 42-43 34.78363927881941 38.0 36.0 38.0 26.0 38.0 44-45 34.824177873760675 38.0 36.0 38.0 26.0 38.0 46-47 34.7497824024083 38.0 36.0 38.0 25.5 38.0 48-49 34.74911488498413 38.0 36.0 38.0 26.0 38.0 50-51 34.678272962272175 38.0 36.0 38.0 25.0 38.0 52-53 34.71723438369163 38.0 36.0 38.0 25.5 38.0 54-55 34.67769379274239 38.0 36.0 38.0 25.0 38.0 56-57 34.61466575046628 38.0 36.0 38.0 25.0 38.0 58-59 34.67707208533752 38.0 36.0 38.0 25.0 38.0 60-61 34.6919374366022 38.0 36.0 38.0 25.0 38.0 62-63 34.53620954811689 38.0 36.0 38.0 25.0 38.0 64-65 34.52709008213082 38.0 36.0 38.0 24.5 38.0 66-67 34.429334773076796 38.0 35.5 38.0 24.5 38.0 68-69 34.35253754785511 38.0 35.0 38.0 24.0 38.0 70-71 34.36064919341645 38.0 35.0 38.0 24.0 38.0 72-73 34.43093485160826 38.0 35.5 38.0 24.0 38.0 74-75 34.464497235038124 38.0 36.0 38.0 24.0 38.0 76 32.78618500703511 37.0 32.0 38.0 20.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16474177489104136 1101 2 0.3389007228068124 1101 3 0.4902917036621197 1101 4 0.4647626268950624 1101 5 -0.5467252458218859 1101 6 -0.08763738815591182 1101 7 -0.2410028361866452 1101 8 -0.31920749560689643 1101 9 -0.6788197130922597 1101 10-11 -0.3069968649805901 1101 12-13 0.23907810020521936 1101 14-15 -0.5573532328909607 1101 16-17 -0.25216479757381904 1101 18-19 -0.11044727884232941 1101 20-21 -0.19124489870222305 1101 22-23 -0.2470220750241907 1101 24-25 -0.46224854779183033 1101 26-27 -0.4756685544001513 1101 28-29 -0.7238712168980186 1101 30-31 -0.27214800520185634 1101 32-33 -0.23289376451878496 1101 34-35 -0.5076911214853581 1101 36-37 -0.21236183273914833 1101 38-39 -0.10402880685772686 1101 40-41 -0.11438667811913916 1101 42-43 -0.3649581180472339 1101 44-45 -0.23415291490315582 1101 46-47 0.03968009580371046 1101 48-49 0.0945068411070551 1101 50-51 -5.687475210791604E-4 1101 52-53 0.06425732276540685 1101 54-55 -0.03817685321899944 1101 56-57 -0.015249498187593247 1101 58-59 0.1341461092486682 1101 60-61 -0.07244441591483053 1101 62-63 0.01073146615348719 1101 64-65 2.3101178096851527E-4 1101 66-67 -0.42023703695802084 1101 68-69 -0.20335243430638883 1101 70-71 -0.2271730770150029 1101 72-73 0.01357886696564492 1101 74-75 0.1334321941202532 1101 76 -0.8029274017039612 1102 1 0.11295977310903993 1102 2 0.21272509663118555 1102 3 0.5138784772488947 1102 4 0.4714352335676679 1102 5 -0.11821816731480794 1102 6 0.5137387132201923 1102 7 8.445056607015999E-4 1102 8 0.4556118292124296 1102 9 0.15972102544847644 1102 10-11 0.20528162229789615 1102 12-13 0.45888802001513795 1102 14-15 0.010813285275553142 1102 16-17 0.14550622509720057 1102 18-19 0.03351619012114071 1102 20-21 0.2986719412146144 1102 22-23 -0.05654340954552595 1102 24-25 0.0026534421101587213 1102 26-27 0.27264101890941816 1102 28-29 0.032301439274633026 1102 30-31 0.31121502816117896 1102 32-33 0.2912127595877436 1102 34-35 -0.0765432153374519 1102 36-37 0.3567154863381745 1102 38-39 0.24928522145630438 1102 40-41 0.18845957472711916 1102 42-43 0.031146228057110648 1102 44-45 0.16184500609475805 1102 46-47 -0.461267830144223 1102 48-49 0.004295475895695233 1102 50-51 0.36267251572018466 1102 52-53 0.589965173473253 1102 54-55 -0.06617160621375717 1102 56-57 0.4237243657862777 1102 58-59 0.008960484063045726 1102 60-61 0.17886238539196597 1102 62-63 0.21095174137376205 1102 64-65 -0.09992986337990573 1102 66-67 0.24558070385972286 1102 68-69 0.42791179695784365 1102 70-71 0.37419312435119423 1102 72-73 0.03973469312147415 1102 74-75 -0.17858889290082658 1102 76 0.17725772848117316 1103 1 0.8429783626565381 1103 2 0.14784992897229188 1103 3 0.2266817339493663 1103 4 0.7403765706032246 1103 5 0.4821489174205844 1103 6 -0.2536095701880541 1103 7 0.373934370827655 1103 8 0.3668902723966525 1103 9 0.5207770999949162 1103 10-11 0.04885175666032637 1103 12-13 0.5771763697810073 1103 14-15 0.225567267909625 1103 16-17 0.14527091436938377 1103 18-19 0.38976251339101253 1103 20-21 0.6780328372778683 1103 22-23 0.5324911105382455 1103 24-25 0.40045328680508874 1103 26-27 -0.061796706106456156 1103 28-29 0.1787658955891942 1103 30-31 0.10431571762160985 1103 32-33 0.38113675422265914 1103 34-35 0.2963701667836389 1103 36-37 0.38894129051258375 1103 38-39 -0.043403674230454214 1103 40-41 0.14768963802570312 1103 42-43 0.041744623237946143 1103 44-45 0.25555279723295854 1103 46-47 0.45044123167191685 1103 48-49 0.04631491256181164 1103 50-51 -0.4506719556542649 1103 52-53 0.12860084187338572 1103 54-55 0.24078653200776756 1103 56-57 0.20586428154503267 1103 58-59 0.12809830555632828 1103 60-61 0.3740079250771231 1103 62-63 0.3492179719312176 1103 64-65 0.5017526083239758 1103 66-67 0.5739780024925594 1103 68-69 0.22747882521866103 1103 70-71 0.3060753748629992 1103 72-73 0.3867968386846883 1103 74-75 0.24988135205763484 1103 76 0.4494840032770355 1104 1 0.35328466560784477 1104 2 -0.12069583026800146 1104 3 0.2835153621031701 1104 4 0.7130979187086162 1104 5 0.2808501556665597 1104 6 0.911970915800218 1104 7 0.4905482801470811 1104 8 -0.25365716266525595 1104 9 0.22603731785172698 1104 10-11 0.20197297442403084 1104 12-13 0.09633426941790901 1104 14-15 0.06433693553833564 1104 16-17 0.0903342231860691 1104 18-19 -0.04097019306089322 1104 20-21 -0.7650404333673251 1104 22-23 -0.036906524691247 1104 24-25 0.1750017459802109 1104 26-27 0.1255554957369398 1104 28-29 0.1351084149076911 1104 30-31 0.23039801908329594 1104 32-33 0.05416068123131623 1104 34-35 -0.08827279011050848 1104 36-37 0.04779288719818453 1104 38-39 0.034533668661275385 1104 40-41 -0.30940234596089056 1104 42-43 0.02765841152582027 1104 44-45 0.06008915325195119 1104 46-47 0.14404750669285704 1104 48-49 -0.22530318631166324 1104 50-51 -4.904083123022929E-4 1104 52-53 -0.08689818056401322 1104 54-55 -0.016398553179833186 1104 56-57 0.41453898594871674 1104 58-59 -0.1386506005045618 1104 60-61 -0.1868686227303442 1104 62-63 -0.0023903513161513956 1104 64-65 -0.06887396275879354 1104 66-67 0.2720857205821332 1104 68-69 0.2139605308326722 1104 70-71 0.18921940242095303 1104 72-73 0.13609159708707352 1104 74-75 -0.022795772977268314 1104 76 0.6808172124754392 1105 1 0.28878394893321513 1105 2 0.08085696476305415 1105 3 0.29190045527086994 1105 4 0.24066600279843797 1105 5 0.5740895249928855 1105 6 0.678573878055353 1105 7 0.33930604412223886 1105 8 -0.056476082875484224 1105 9 0.0014792672067187596 1105 10-11 0.4063805233967983 1105 12-13 -0.5367163755892577 1105 14-15 0.3657583402206086 1105 16-17 -0.09185641226543595 1105 18-19 0.15549421209916403 1105 20-21 0.29427633681901 1105 22-23 0.2687313157291982 1105 24-25 0.19166443112115417 1105 26-27 0.3078058540742532 1105 28-29 -0.036929329956137735 1105 30-31 -0.17669705975090721 1105 32-33 -0.2098861415111557 1105 34-35 0.23444579565155266 1105 36-37 -0.32899879937611587 1105 38-39 0.03719730936838772 1105 40-41 -0.07527668900915074 1105 42-43 0.049827546738427486 1105 44-45 -0.02277037852062591 1105 46-47 0.4123585434821564 1105 48-49 0.2757240473242675 1105 50-51 0.014320867368539325 1105 52-53 -0.06388098037289325 1105 54-55 -0.031006771048922133 1105 56-57 0.11273535479725894 1105 58-59 0.41005938516195073 1105 60-61 0.5316096381392157 1105 62-63 0.20655613697816477 1105 64-65 -0.3614683249183699 1105 66-67 0.18624004451906018 1105 68-69 0.31362608267212977 1105 70-71 0.23243488259295475 1105 72-73 0.31995447334125515 1105 74-75 0.08075176643983184 1105 76 -0.2535115022880632 1106 1 -0.12965556362394182 1106 2 0.31272749075122874 1106 3 0.6002104459338042 1106 4 0.8037486717634614 1106 5 0.5668736471887712 1106 6 0.47789873620374124 1106 7 -0.09194201065523089 1106 8 0.5780279751579869 1106 9 0.5327153513839846 1106 10-11 0.02877272814194498 1106 12-13 0.6202828343511229 1106 14-15 0.47071895694593735 1106 16-17 0.8966972998176814 1106 18-19 0.7661719874828208 1106 20-21 0.7268938282600388 1106 22-23 0.6178059883332878 1106 24-25 0.6364751759318992 1106 26-27 0.4867423859519633 1106 28-29 0.39533622583883243 1106 30-31 0.16297829757150595 1106 32-33 0.24799889284445698 1106 34-35 0.36111150467020536 1106 36-37 0.6181893066355144 1106 38-39 0.36752362793000515 1106 40-41 0.6622870544369519 1106 42-43 0.3542758217749409 1106 44-45 0.4379971763645756 1106 46-47 0.6841701741173125 1106 48-49 0.5025693153460011 1106 50-51 0.31813230647409796 1106 52-53 0.8416302839618979 1106 54-55 0.06480112475897215 1106 56-57 0.5520899000341544 1106 58-59 0.44551630297180367 1106 60-61 0.5889081130847558 1106 62-63 0.4009084078010261 1106 64-65 0.7853175689263523 1106 66-67 0.34134071726679593 1106 68-69 0.5040903025481143 1106 70-71 0.5468067852001468 1106 72-73 0.5971624505492343 1106 74-75 0.3552495997611942 1106 76 0.7888261165201484 1107 1 -0.2692849484615252 1107 2 -0.2934608522513926 1107 3 -0.05031463301163441 1107 4 -0.7764966098697919 1107 5 -0.08932571145605905 1107 6 -0.9724941550351502 1107 7 -0.3958464438392397 1107 8 -0.3544387943213394 1107 9 -0.09423625322789775 1107 10-11 -0.30922634164826945 1107 12-13 0.22416768417119926 1107 14-15 0.08892169372104064 1107 16-17 -0.13818311926630145 1107 18-19 0.025712757598604696 1107 20-21 0.1584817942828991 1107 22-23 -0.21624213778358126 1107 24-25 -0.11208911869981364 1107 26-27 -0.33675520284635496 1107 28-29 -0.024063544056645014 1107 30-31 -0.3992307058800648 1107 32-33 -0.3715110334731335 1107 34-35 0.03197635497986795 1107 36-37 -0.0686580165634183 1107 38-39 -0.3044868491696988 1107 40-41 -0.02944387014262162 1107 42-43 0.103525534144282 1107 44-45 -0.2794687363425794 1107 46-47 -0.013829874841093215 1107 48-49 0.06773540675135337 1107 50-51 -0.08988952897669122 1107 52-53 0.09157030990985504 1107 54-55 -0.05109567091309941 1107 56-57 0.08204020724817696 1107 58-59 0.10626879373090503 1107 60-61 -0.21895215961359327 1107 62-63 -0.009779213065058912 1107 64-65 0.10103321848205127 1107 66-67 -0.023822926218066698 1107 68-69 -0.001672102288971189 1107 70-71 0.13301520115079768 1107 72-73 0.13153615009147046 1107 74-75 0.1487601625381174 1107 76 -0.43459558674292964 1108 1 -0.035056575798176226 1108 2 -0.08649906450834166 1108 3 0.11869014664853594 1108 4 0.35607264515385495 1108 5 0.5684506204675657 1108 6 0.7234844240794871 1108 7 0.6442376381406874 1108 8 0.21890021120905345 1108 9 -0.06924688859738382 1108 10-11 0.34453857914950703 1108 12-13 0.2542770407939159 1108 14-15 0.2320937865115198 1108 16-17 0.3271430737719143 1108 18-19 0.5887867547458256 1108 20-21 0.008402967426711427 1108 22-23 0.20951547588975927 1108 24-25 -0.017694584028504323 1108 26-27 0.5267417066404008 1108 28-29 0.19945188348521015 1108 30-31 0.5791682330854258 1108 32-33 0.39199838821301825 1108 34-35 0.45168557146594424 1108 36-37 0.2714711093343283 1108 38-39 0.4393583999481905 1108 40-41 0.4735815796709488 1108 42-43 0.5913361494942535 1108 44-45 -0.008824858561744975 1108 46-47 0.5740683259046335 1108 48-49 0.05649694925529758 1108 50-51 0.40647192343941896 1108 52-53 0.36664350627631137 1108 54-55 0.6405661994104648 1108 56-57 0.5425892425576038 1108 58-59 0.21092333190562584 1108 60-61 0.2059971659788573 1108 62-63 0.3411629555493505 1108 64-65 0.6255871585736443 1108 66-67 1.0074275429894115 1108 68-69 0.3951748234636412 1108 70-71 0.4987042479714461 1108 72-73 -0.012133928234895563 1108 74-75 0.28622669240473186 1108 76 0.3608549775572598 1109 1 -0.3306384890938716 1109 2 -0.08525599204757484 1109 3 -0.24696997662281817 1109 4 -0.2816442450932044 1109 5 0.14025359348253374 1109 6 -0.10238702151019652 1109 7 0.19777780491958197 1109 8 -0.35294937702319373 1109 9 0.0026803916636595204 1109 10-11 0.020653459762755233 1109 12-13 -0.6007081977206141 1109 14-15 -0.1778015882230406 1109 16-17 0.051077398110230376 1109 18-19 -0.1564403649516919 1109 20-21 -0.14891303195174999 1109 22-23 0.04656162495486171 1109 24-25 -0.10409329952030077 1109 26-27 0.024493817273850027 1109 28-29 -0.4094568024836036 1109 30-31 -0.003019574445517037 1109 32-33 -0.3699040305988177 1109 34-35 -0.42078037352810327 1109 36-37 -0.43974502988978514 1109 38-39 -0.28692740312840925 1109 40-41 -0.32413945415098055 1109 42-43 -0.09787242770573101 1109 44-45 -0.4973678740483578 1109 46-47 -0.2652800969471798 1109 48-49 -0.574595400669601 1109 50-51 -0.4669211464316163 1109 52-53 -0.3303006072576409 1109 54-55 -0.1393763084882238 1109 56-57 -0.441098021129136 1109 58-59 -0.3666081333660358 1109 60-61 -0.22769268744217186 1109 62-63 -0.38512543726155 1109 64-65 -0.34278700623705305 1109 66-67 -0.007818222795016538 1109 68-69 -0.1497907330702688 1109 70-71 -0.2026916188126151 1109 72-73 -0.5396161885549873 1109 74-75 -0.20841255760821298 1109 76 -0.5255023021858349 1110 1 -0.3898859660574523 1110 2 -0.5926605554525786 1110 3 0.09028320459701789 1110 4 -0.1361637712011472 1110 5 -0.10534754736871577 1110 6 0.004874935488487608 1110 7 -0.5473516378184655 1110 8 -0.20704108391218057 1110 9 0.23440483220775832 1110 10-11 -0.3462837934184648 1110 12-13 0.05441466318328736 1110 14-15 -0.3518033433788119 1110 16-17 -0.2531953092175385 1110 18-19 -0.6241838928147914 1110 20-21 -0.31168727504082483 1110 22-23 -0.38485566332947485 1110 24-25 -0.03752901694021915 1110 26-27 -0.3384723959775826 1110 28-29 0.01955263147865338 1110 30-31 -0.04446867492818285 1110 32-33 0.004591362117288611 1110 34-35 -0.24009805672248774 1110 36-37 0.011779761685460244 1110 38-39 -0.013046314931834502 1110 40-41 0.03539872048701653 1110 42-43 -0.07015742112389489 1110 44-45 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0.28274795838937905 2116 9 -0.32052118132433804 2116 10-11 -0.07170873428430014 2116 12-13 0.11414704670273323 2116 14-15 -0.07434033661275663 2116 16-17 0.16501732624094956 2116 18-19 0.1600692289190775 2116 20-21 0.08582152632338591 2116 22-23 0.43838818946771596 2116 24-25 0.10651080923283729 2116 26-27 -0.13007259400827564 2116 28-29 0.23864815378460946 2116 30-31 0.22561287521208584 2116 32-33 -0.18142684570492662 2116 34-35 0.3017703078740226 2116 36-37 0.034670184701028006 2116 38-39 0.22421233515893135 2116 40-41 -0.24632400903605856 2116 42-43 -0.11859586658294319 2116 44-45 0.0696942996583374 2116 46-47 0.29188310055773314 2116 48-49 0.2424430426146884 2116 50-51 0.17879720735508187 2116 52-53 -0.13569080320312565 2116 54-55 0.2931242000208272 2116 56-57 0.023567379915000686 2116 58-59 0.055271316088159494 2116 60-61 -0.05702009947010822 2116 62-63 0.17065122556304857 2116 64-65 -0.09030887212626482 2116 66-67 -0.04802772852422521 2116 68-69 -0.045983695304990135 2116 70-71 -0.05126101334784039 2116 72-73 0.2640450766155311 2116 74-75 0.05496123044521539 2116 76 0.3729518302977226 2117 1 0.9454505021941984 2117 2 0.4147800076644259 2117 3 0.3322365897246513 2117 4 0.2653929786090039 2117 5 0.3118570206551148 2117 6 0.9901106385085257 2117 7 1.1755731825565547 2117 8 0.002864576465178459 2117 9 0.3803245705102256 2117 10-11 0.5971708476236586 2117 12-13 -0.1886266943726369 2117 14-15 0.7114596976866707 2117 16-17 0.621384835650737 2117 18-19 0.7067751293243489 2117 20-21 -0.05512658224335354 2117 22-23 0.3556227524658411 2117 24-25 0.4995642712191355 2117 26-27 0.7045499768245733 2117 28-29 0.7462707516959597 2117 30-31 0.7998892022842625 2117 32-33 0.5034741823754771 2117 34-35 0.4492894659131821 2117 36-37 0.3209756843445035 2117 38-39 0.43362843242490356 2117 40-41 0.5628718497585865 2117 42-43 0.4399884694070906 2117 44-45 0.17306196251295347 2117 46-47 0.7138112703218837 2117 48-49 0.5805993577723356 2117 50-51 0.72846369048969 2117 52-53 0.5265861376484011 2117 54-55 0.6881814710495391 2117 56-57 -0.018544201559699047 2117 58-59 0.3974713539980641 2117 60-61 0.40653223875841604 2117 62-63 0.45778261947399557 2117 64-65 0.010463767756753839 2117 66-67 0.4793982794729672 2117 68-69 0.11405475616582095 2117 70-71 0.6324620999576354 2117 72-73 -0.7818078510216893 2117 74-75 0.04416142179994864 2117 76 -0.08722020844258793 2118 1 0.11009820514680158 2118 2 0.005113915865642582 2118 3 0.03456584357384429 2118 4 -0.02982592138475937 2118 5 0.019557479856811 2118 6 -0.03346240780643228 2118 7 0.5415112214951989 2118 8 0.28138806639806546 2118 9 0.5346454299030867 2118 10-11 -0.6950871364601241 2118 12-13 0.28347316641236375 2118 14-15 0.48129783428359474 2118 16-17 0.25323909625289076 2118 18-19 0.20224544744100115 2118 20-21 0.2592811306828722 2118 22-23 0.46682852053781687 2118 24-25 0.11540880755009653 2118 26-27 0.15453127630303953 2118 28-29 -0.06784606422857564 2118 30-31 0.08209046145272225 2118 32-33 0.09752222193747428 2118 34-35 0.33393322097791156 2118 36-37 0.5177155600899255 2118 38-39 0.1216725656557287 2118 40-41 0.27241119357806554 2118 42-43 0.3770456306679222 2118 44-45 0.40903137677106116 2118 46-47 -0.16990784784464097 2118 48-49 0.11074875416105101 2118 50-51 0.28020699466406285 2118 52-53 0.09269767587019118 2118 54-55 0.296841890356788 2118 56-57 0.14276338046286696 2118 58-59 -0.1538096317272064 2118 60-61 0.16952910122646614 2118 62-63 -0.19202414180345784 2118 64-65 -0.050505127042420384 2118 66-67 0.007532175207167313 2118 68-69 -0.03781945165930978 2118 70-71 0.06332211616475547 2118 72-73 0.2814154955405286 2118 74-75 0.3553848514755984 2118 76 0.4604349539402719 2119 1 0.07180093195020021 2119 2 -0.15290926900317814 2119 3 -0.26833930496889025 2119 4 -0.1718215096890745 2119 5 0.6429206938240526 2119 6 0.20005239953387388 2119 7 0.5528924577086514 2119 8 0.020347093947698625 2119 9 0.40837237409982663 2119 10-11 0.35298392000019874 2119 12-13 0.18785904898616224 2119 14-15 0.4353387598010343 2119 16-17 0.22667505626603912 2119 18-19 -0.3041960975911451 2119 20-21 0.11195865450132914 2119 22-23 0.2350150320129245 2119 24-25 0.3149911044478273 2119 26-27 0.0033602996287029896 2119 28-29 0.5821515891247842 2119 30-31 0.18653970348585602 2119 32-33 0.08242155079653202 2119 34-35 0.282447793653553 2119 36-37 0.18863356825625033 2119 38-39 0.1274570496281342 2119 40-41 -0.1854664991989594 2119 42-43 1.2724703812949656E-4 2119 44-45 0.22268416693392368 2119 46-47 -0.1533757222521075 2119 48-49 0.16373603533624959 2119 50-51 0.1730121760598422 2119 52-53 0.13811702162510642 2119 54-55 -0.17865911870126183 2119 56-57 0.189508581570486 2119 58-59 0.25321622831879154 2119 60-61 0.051539708069284984 2119 62-63 -0.1549823245602937 2119 64-65 -0.036143649593689986 2119 66-67 -0.16805565977664827 2119 68-69 -0.77029000124395 2119 70-71 -0.5080746079165337 2119 72-73 -0.41151405812728115 2119 74-75 0.07850401419207742 2119 76 0.09773724896069069 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 13.0 13 194.0 14 674.0 15 1216.0 16 1462.0 17 1694.0 18 1618.0 19 1546.0 20 1244.0 21 949.0 22 909.0 23 877.0 24 918.0 25 1053.0 26 1260.0 27 1497.0 28 1896.0 29 2242.0 30 2902.0 31 3533.0 32 4632.0 33 6233.0 34 8867.0 35 13606.0 36 26325.0 37 65444.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.31877229148261 23.34413140931252 12.207061287261542 26.130035011943324 2 19.566113674290765 21.102712607571743 32.8300775498184 26.501096168319098 3 19.33575472006806 23.433788161382154 28.793560420143322 28.436896698406468 4 14.941265010961683 18.964693563692286 34.848990543503156 31.24505088184287 5 15.384967769379273 33.54733156637544 33.872582703445566 17.19511796079971 6 28.66791008147639 34.41052321586335 18.117862635384967 18.803704067275284 7 27.325676515820817 27.425804129446025 23.25643794378456 21.992081410948593 8 24.20732305880043 31.959687183011027 20.29383855240339 23.53915120578515 9 24.001832400772226 17.03609175092438 22.3723045711855 36.5897712771179 10-11 25.82932495664409 24.434409868787014 25.718399267039693 24.017865907529202 12-13 25.022086973593794 22.188410065115672 25.22954091816367 27.559962043126863 14-15 23.584306796243578 23.424298943097412 23.507084192271197 29.484310068387813 16-17 24.09345243938353 25.36206275972645 24.759333791433527 25.7851510094565 18-19 23.371944635319526 24.7698046529891 25.94483164817905 25.913419063512322 20-21 23.006773338568763 25.379732338601485 25.828997742220476 25.78449658060927 22-23 24.728248421190408 25.060371061156378 25.07640456791335 25.134975949739864 24-25 23.88698013808449 25.695167042963252 24.90428978109355 25.51356303785871 26-27 23.11540852720788 25.95922908281797 25.585550211053302 25.339812178920845 28-29 23.655312326167337 25.226923202774778 26.6008965675207 24.516867903537186 30-31 23.861457413042768 24.923268217663033 26.22885376787409 24.98642060142011 32-33 22.954091816367264 25.835869245116328 26.173554530283695 25.036484408232717 34-35 23.485488040312816 25.310362880795783 26.356794607506302 24.8473544713851 36-37 23.823173325480187 24.95468080232977 26.104839501325216 25.117306370864828 38-39 22.994666404895128 25.425542357907137 26.800824580347506 24.778966656850233 40-41 23.128497104152352 24.664441608586106 27.190209744445536 25.01685154281601 42-43 22.63702103988744 24.69290926344033 26.94479892673669 25.725270769935538 44-45 22.225385294983802 24.54664441608586 27.528549458460127 25.69942083047021 46-47 22.23225679787965 24.69585419325284 27.754654625175878 25.317234383691634 48-49 22.044762933150093 24.754752789502962 27.719642681849415 25.480841595497527 50-51 21.996335198455547 25.32868688851805 27.118877000098163 25.556100912928244 52-53 21.47279212067668 25.087202643892542 27.420568698668234 26.01943653676254 54-55 21.253231242433166 25.17326003730244 27.274631065737374 26.298877654527015 56-57 21.441052321586334 25.02994011976048 27.20002617715389 26.328981381499293 58-59 21.55328686888518 25.001799679329867 27.011550669153493 26.43336278263146 60-61 21.51303949478093 24.594090507509573 27.16632309152188 26.726546906187625 62-63 21.32489120120415 24.63793724027355 27.1142959981676 26.922875560354704 64-65 21.930565099309575 24.866987336801806 26.038742187755638 27.16370537613298 66-67 21.887045580969207 24.843100683878145 25.687641111220184 27.582212623932463 68-69 21.64654297961454 25.024050260135468 25.879061549033082 27.45034521121691 70-71 22.617388174470733 24.8251038905795 25.492621314747556 27.064886620202216 72-73 22.6026635254082 24.68538333169726 25.210235267170578 27.501717875723962 74-75 22.31929583456039 24.54108177088446 25.858774254769152 27.280848139786002 76 22.44690945976899 24.056804423939006 25.022086973593794 28.474199142698208 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 1.5 8 3.0 9 1.5 10 0.5 11 3.5 12 6.0 13 6.0 14 5.5 15 7.5 16 18.5 17 27.0 18 35.5 19 44.0 20 53.0 21 62.0 22 85.0 23 151.5 24 260.0 25 325.0 26 455.0 27 658.5 28 827.0 29 922.0 30 1082.0 31 1425.5 32 1986.0 33 2363.0 34 2584.5 35 3255.0 36 3907.5 37 4111.0 38 4679.0 39 5922.5 40 7115.5 41 7927.0 42 8221.0 43 8359.5 44 8886.5 45 9258.0 46 9241.0 47 9222.5 48 8888.5 49 8065.0 50 7557.0 51 7044.0 52 5906.5 53 4902.5 54 4523.0 55 4240.5 56 3543.0 57 2855.0 58 2582.0 59 2352.0 60 2017.5 61 1871.5 62 1830.0 63 1841.5 64 1929.0 65 2054.5 66 2143.0 67 2182.0 68 2219.5 69 2234.0 70 2187.0 71 2163.0 72 2052.5 73 1837.0 74 1511.5 75 1291.0 76 1112.0 77 746.5 78 458.5 79 357.0 80 275.5 81 129.5 82 54.5 83 44.0 84 27.5 85 10.0 86 7.5 87 6.0 88 5.0 89 4.0 90 5.0 91 4.5 92 3.0 93 3.0 94 3.0 95 3.0 96 3.0 97 4.5 98 4.5 99 14.0 100 25.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 152805.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.87382611825529 #Duplication Level Percentage of deduplicated Percentage of total 1 74.56838998336808 41.66421255848958 2 12.537187565883483 14.01001276136252 3 4.522242263815034 7.580249337390792 4 2.2113425004099416 4.942246654232518 5 1.3832603246738036 3.8644023428552727 6 0.9569209866710393 3.2080102090900167 7 0.7683478179390475 3.005137266450705 8 0.6324814354986062 2.82713262000589 9 0.4767036004591347 2.397172867379994 >10 1.9407809974466492 16.42943620954812 >50 0.002342523835180023 0.07198717319459441 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.003272144236117928 0.0 2 0.0 0.0 0.0 0.003926573083341513 0.0 3 0.0 0.0 0.0 0.003926573083341513 0.0 4 0.0 0.0 0.0 0.018324007722260397 0.0 5 0.0 0.0 0.0 0.018978436569483982 0.0 6 0.0 0.0 0.0 0.018978436569483982 0.0 7 0.0 0.0 0.0 0.018978436569483982 0.0 8 0.0 0.0 0.0 0.020287294263931156 0.0 9 0.0 0.0 0.0 0.02225058080560191 0.0 10 0.0 0.0 0.0 0.022905009652825496 0.0 11 0.0 0.0 0.0 0.022905009652825496 0.0 12 0.0 0.0 0.0 0.02355943850004908 0.0 13 0.0 0.0 0.0 0.02421386734727267 0.0 14 0.0 0.0 0.0 0.024868296194496254 0.0 15 0.0 0.0 0.0 0.02552272504171984 0.0 16 0.0 0.0 0.0 0.026177153888943424 0.0 17 0.0 0.0 0.0 0.02683158273616701 0.0 18 0.0 0.0 0.0 0.028140440430614182 0.0 19 0.0 0.0 0.0 0.029449298125061352 0.0 20 0.0 0.0 0.0 0.030103726972284937 0.0 21 0.0 0.0 0.0 0.032067013513955696 0.0 22 0.0 0.0 0.0 0.035993586597297206 0.0 23 0.0 0.0 0.0 0.03730244429174438 0.0 24 0.0 0.0 0.0 0.04122901737508589 0.0 25 0.0 0.0 0.0 0.04253787506953306 0.0 26 0.0 0.0 0.0 0.04908216354176892 0.0 27 0.0 0.0 0.0 0.0818036059029482 0.0 28 0.0 0.0 0.0 0.12041490788913975 0.0 29 0.0 0.0 0.0 0.1596806387225549 0.0 30 0.0 0.0 0.0 0.19894636955597003 0.0 31 0.0 0.0 0.0 0.24737410425051537 0.0 32 0.0 0.0 0.0 0.309544844736756 0.0 33 0.0 0.0 0.0 0.37956873138967967 0.0 34 0.0 0.0 0.0 0.4914760642649128 0.0 35 0.0 0.0 0.0 0.6354504106541017 0.0 36 0.0 0.0 0.0 0.8330879225156245 0.0 37 0.0 0.0 0.0 1.132161905696803 0.0 38 0.0 0.0 0.0 1.528745787114296 0.0 39 0.0 0.0 0.0 1.899806943490069 0.0 40 0.0 0.0 0.0 2.325840123032623 0.0 41 0.0 0.0 0.0 2.836949052714244 0.0 42 0.0 0.0 0.0 3.4305160171460356 0.0 43 0.0 0.0 0.0 4.039134845063971 0.0 44 0.0 0.0 0.0 4.61764994600962 0.0 45 0.0 0.0 0.0 5.173914466149668 0.0 46 0.0 0.0 0.0 5.782533294067602 0.0 47 0.0 0.0 0.0 6.351886391152122 0.0 48 0.0 0.0 0.0 6.876738326625437 0.0 49 0.0 0.0 0.0 7.417950983279343 0.0 50 0.0 0.0 0.0 7.964399070711037 0.0 51 0.0 0.0 0.0 8.555348319753934 0.0 52 0.0 0.0 0.0 9.195379732338601 0.0 53 0.0 0.0 0.0 9.858970583423318 0.0 54 0.0 0.0 0.0 10.47282484211904 0.0 55 0.0 0.0 0.0 10.978043912175648 0.0 56 0.0 0.0 0.0 11.498969274565622 0.0 57 0.0 0.0 0.0 12.044762933150093 0.0 58 0.0 0.0 0.0 12.555871862831713 0.0 59 0.0 0.0 0.0 13.069598507902228 0.0 60 0.0 0.0 0.0 13.6265174568895 0.0 61 0.0 0.0 0.0 14.203723700140703 0.0 62 0.0 0.0 0.0 14.744936356794607 0.0 63 0.0 0.0 0.0 15.3385033212264 0.0 64 0.0 0.0 0.0 15.895422270213672 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGATTA 30 8.345514E-6 58.333332 37 GATCGAA 20 0.006940563 52.500004 30 CTAAGGG 20 0.006940563 52.500004 36 CTTATTG 20 0.006940563 52.500004 40 CTTATAA 20 0.006940563 52.500004 1 AGCTAGA 20 0.006940563 52.500004 1 AGGACCG 30 5.863326E-4 46.666664 5 GTAGGTA 30 5.863326E-4 46.666664 47 GTAGATT 40 4.5760342E-5 43.750004 36 GGTGCTA 45 9.1644935E-5 38.88889 58 AGCCCGG 40 0.002412896 35.000004 30 AAAGGAG 40 0.002412896 35.000004 5 GTGCTAA 50 1.7035654E-4 35.0 59 AGTGTGA 55 2.9814086E-4 31.81818 19 GAGGACC 45 0.0042968923 31.111113 4 CGGGCTA 45 0.0042968923 31.111113 41 GTTAGAT 60 4.964334E-4 29.166666 3 TAACCGT 50 0.0071911262 27.999998 4 CATAGAC 50 0.0071911262 27.999998 2 TCTCGTT 50 0.0071911262 27.999998 43 >>END_MODULE