FastQCFastQC Report
Wed 25 May 2016
SRR1780352_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780352_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152805
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT36922.416151303949478No Hit
CACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTTCTG18211.1917149307941495TruSeq Adapter, Index 2 (95% over 24bp)
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG9240.6046922548345931No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC8760.5732796701678611No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC3090.20221851379208797No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT2140.14004777330584733TruSeq Adapter, Index 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA200.00694056352.50000440
TTAATAC200.00694056352.5000043
GTCTCTC200.00694056352.50000467
GAGAAAC200.00694056352.50000419
CGCTAAT200.00694056352.50000448
AAGGCAT200.00694056352.50000457
ATCGCAT200.00694056352.50000439
GTGCTAA305.863326E-446.6666641
CTAATGG305.863326E-446.6666644
CGAATTC350.001252355440.041
TCTTTAT806.1907485E-835.0000041
CTTTATA904.454705E-935.0000042
TAGAAGC501.7035654E-435.059
AAGCCGG601.2096694E-535.062
GCGTAAA501.7035654E-435.068
GGCGTAA552.9814086E-431.8181867
GACAGAC450.004296892331.11111330
CTAACTT450.004296892331.11111321
TGACAGA450.004296892331.11111329
AAAATTG450.004296892331.11111361