##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780352_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 152805 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.53087922515625 34.0 34.0 34.0 34.0 34.0 2 33.60852066359085 34.0 34.0 34.0 34.0 34.0 3 33.65002454108177 34.0 34.0 34.0 34.0 34.0 4 33.67917934622558 34.0 34.0 34.0 34.0 34.0 5 33.63569909361605 34.0 34.0 34.0 34.0 34.0 6 37.29203887307352 38.0 38.0 38.0 36.0 38.0 7 37.3142240109944 38.0 38.0 38.0 36.0 38.0 8 37.13140931252249 38.0 38.0 38.0 36.0 38.0 9 37.20702202153071 38.0 38.0 38.0 36.0 38.0 10-11 37.13282615097673 38.0 38.0 38.0 36.0 38.0 12-13 37.12419096233762 38.0 38.0 38.0 36.0 38.0 14-15 37.02748273943915 38.0 38.0 38.0 36.0 38.0 16-17 37.06820457445764 38.0 38.0 38.0 35.5 38.0 18-19 37.05305454664442 38.0 38.0 38.0 35.5 38.0 20-21 37.06029580183895 38.0 38.0 38.0 36.0 38.0 22-23 37.02390955793331 38.0 37.5 38.0 35.5 38.0 24-25 37.03796341742744 38.0 38.0 38.0 35.5 38.0 26-27 36.91506822420732 38.0 37.5 38.0 35.0 38.0 28-29 36.86888190831452 38.0 37.0 38.0 35.0 38.0 30-31 36.82578122443637 38.0 37.0 38.0 35.0 38.0 32-33 36.79644645135957 38.0 37.0 38.0 34.5 38.0 34-35 36.83124243316645 38.0 37.0 38.0 35.0 38.0 36-37 36.809819704852586 38.0 37.0 38.0 35.0 38.0 38-39 36.513363437060306 38.0 37.0 38.0 34.0 38.0 40-41 36.66557049834756 38.0 37.0 38.0 34.0 38.0 42-43 36.73964857170904 38.0 37.0 38.0 34.5 38.0 44-45 36.80690422433821 38.0 37.0 38.0 35.0 38.0 46-47 36.72910245083604 38.0 37.0 38.0 34.5 38.0 48-49 36.72542783285887 38.0 37.0 38.0 34.0 38.0 50-51 36.56815876443834 38.0 37.0 38.0 34.0 38.0 52-53 36.60576879028828 38.0 37.0 38.0 34.0 38.0 54-55 36.54718431988482 38.0 37.0 38.0 34.0 38.0 56-57 36.607489938156476 38.0 37.0 38.0 34.0 38.0 58-59 36.5145774025719 38.0 37.0 38.0 34.0 38.0 60-61 36.51915840450247 38.0 37.0 38.0 34.0 38.0 62-63 36.49516704296325 38.0 37.0 38.0 34.0 38.0 64-65 36.4430516017146 38.0 37.0 38.0 34.0 38.0 66-67 36.18963384705998 38.0 37.0 38.0 33.5 38.0 68-69 36.14550898203593 38.0 37.0 38.0 34.0 38.0 70-71 35.79663623572527 38.0 37.0 38.0 32.0 38.0 72-73 35.396037433330065 38.0 37.0 38.0 29.5 38.0 74-75 35.25121887372796 38.0 37.0 38.0 27.0 38.0 76 34.41291188115572 38.0 35.0 38.0 24.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05899199294293567 1101 2 0.060692756321323316 1101 3 0.12395337592028 1101 4 0.015521503195557784 1101 5 -0.0020239811967925903 1101 6 0.04933937149710488 1101 7 0.13286368779739632 1101 8 0.0998971375171962 1101 9 0.19255888957455625 1101 10-11 0.14795856711668165 1101 12-13 0.16762412671489813 1101 14-15 0.3279892716414068 1101 16-17 0.3396354808657094 1101 18-19 0.19220854132883147 1101 20-21 0.2557682842474023 1101 22-23 0.19585460089127338 1101 24-25 0.19366123964355353 1101 26-27 0.10675952625386742 1101 28-29 -0.14358186306672138 1101 30-31 0.24197608044634222 1101 32-33 0.021514512970775 1101 34-35 0.18411610808505685 1101 36-37 0.3653357318791066 1101 38-39 0.31246124616802007 1101 40-41 0.29391965281988774 1101 42-43 0.42603600302975053 1101 44-45 0.38039706807203544 1101 46-47 0.11313880822433475 1101 48-49 0.26463253314855706 1101 50-51 0.31814281472785666 1101 52-53 0.19132397281082092 1101 54-55 0.22791271073326413 1101 56-57 0.21973582415935056 1101 58-59 0.1765813073648843 1101 60-61 0.1490137919217105 1101 62-63 0.24671786555470732 1101 64-65 0.15088325966399196 1101 66-67 0.3644615251104355 1101 68-69 -0.20951143015997076 1101 70-71 0.17459064488948428 1101 72-73 -0.07550875159433446 1101 74-75 0.09272764288107993 1101 76 -0.17897503030853557 1102 1 0.1394493734003177 1102 2 -0.003885008256446554 1102 3 -0.024447772480868935 1102 4 0.07418401185806545 1102 5 0.10548022630741372 1102 6 0.14479526695300393 1102 7 -0.0824763775426689 1102 8 0.008955646575707021 1102 9 -0.25620360918794916 1102 10-11 0.05147802063613227 1102 12-13 0.15727614136691415 1102 14-15 0.23611470476684104 1102 16-17 0.17708721831745322 1102 18-19 0.210248834369132 1102 20-21 0.2854262389053659 1102 22-23 0.09346127349794386 1102 24-25 0.05670202768433086 1102 26-27 0.2501438446381883 1102 28-29 0.1928402733554151 1102 30-31 0.4257474892177484 1102 32-33 0.2796250960813538 1102 34-35 0.2406228895918403 1102 36-37 0.5079775495209233 1102 38-39 0.16566884937562065 1102 40-41 0.21044771934794682 1102 42-43 0.3043970563907976 1102 44-45 0.3027958404708144 1102 46-47 0.3622502823358147 1102 48-49 0.422265440781473 1102 50-51 0.22825814984319237 1102 52-53 0.5186381001249458 1102 54-55 0.28907497189553055 1102 56-57 0.28730091672444047 1102 58-59 0.4996805054640774 1102 60-61 0.4462467391546525 1102 62-63 0.44654956538640533 1102 64-65 0.42541053419127195 1102 66-67 0.6974112330601443 1102 68-69 0.5531605825120494 1102 70-71 0.41434165964049896 1102 72-73 0.4075325314469467 1102 74-75 0.33531006046349177 1102 76 0.2938296424961351 1103 1 -0.023159751949705765 1103 2 0.1903781161993976 1103 3 0.098643269239723 1103 4 -0.047179849120354334 1103 5 -0.04873771848868813 1103 6 0.029083569656719988 1103 7 0.05275199151867582 1103 8 0.04213916541232976 1103 9 -0.1088426190004057 1103 10-11 -0.042749807210533675 1103 12-13 0.1650366891702859 1103 14-15 0.2392278656958453 1103 16-17 0.08519367289071056 1103 18-19 0.0459737090661676 1103 20-21 0.12015810233101831 1103 22-23 0.25599745252234385 1103 24-25 0.0012769388545379456 1103 26-27 0.04028432514269298 1103 28-29 -0.088071320404147 1103 30-31 -0.11948629199033434 1103 32-33 0.19839347973196908 1103 34-35 -0.019437820575937792 1103 36-37 0.15689629672660743 1103 38-39 -0.11671578954006634 1103 40-41 0.003957849474780062 1103 42-43 0.13083421666093642 1103 44-45 0.35945160440709145 1103 46-47 -0.0263489129014971 1103 48-49 0.016166186716475295 1103 50-51 -0.02167478659936961 1103 52-53 0.07585625670070328 1103 54-55 0.19872506484326635 1103 56-57 -0.02397282024521985 1103 58-59 0.2542984784974749 1103 60-61 0.2642644815835311 1103 62-63 0.027472924682356847 1103 64-65 0.33551007062913385 1103 66-67 -0.16350836126415658 1103 68-69 -0.09468514777479697 1103 70-71 0.1728375534682982 1103 72-73 0.3575313548933039 1103 74-75 0.18881971996887614 1103 76 0.3370656356250663 1104 1 0.1018239553401159 1104 2 -0.1170001539802854 1104 3 -0.0964735727675432 1104 4 0.12719404530287903 1104 5 0.08861448335471778 1104 6 0.08805852543365233 1104 7 -0.06114336273139287 1104 8 -0.5076234265251074 1104 9 0.09888478068305062 1104 10-11 0.060532010124909164 1104 12-13 -0.16969471386480706 1104 14-15 0.08879029005111505 1104 16-17 0.1527083363298729 1104 18-19 0.07844042538680895 1104 20-21 0.16533546011893208 1104 22-23 0.10723337096569452 1104 24-25 0.058075654057965664 1104 26-27 0.25919783412695807 1104 28-29 0.18787132925603345 1104 30-31 0.004892257492947749 1104 32-33 -0.05881255322585588 1104 34-35 0.17391242614225177 1104 36-37 0.07767415726100779 1104 38-39 -0.18865508755700944 1104 40-41 0.06186912403021694 1104 42-43 0.14846060154130214 1104 44-45 0.11598265365763183 1104 46-47 -0.01798144246113509 1104 48-49 0.1381636729840494 1104 50-51 -0.019675438307778848 1104 52-53 0.06639251961849624 1104 54-55 -0.22885383842458396 1104 56-57 -0.3699136078813794 1104 58-59 0.2904473473178797 1104 60-61 0.18801692549005367 1104 62-63 0.2016140661030832 1104 64-65 -0.07104192639162932 1104 66-67 -0.16214442866942846 1104 68-69 0.012178738269334133 1104 70-71 -0.20230908092328548 1104 72-73 0.12904471491565062 1104 74-75 -0.043141922336310756 1104 76 0.5315028388649878 1105 1 0.09549332944427391 1105 2 0.06644466207322353 1105 3 0.012914864881764743 1105 4 -0.007134669460619136 1105 5 0.26591978674697714 1105 6 0.08105900321673687 1105 7 0.06477636971008138 1105 8 0.16939520701526334 1105 9 0.16577441279007843 1105 10-11 0.1679615371196519 1105 12-13 0.33310031719108935 1105 14-15 0.3207300893822307 1105 16-17 0.11444985568008548 1105 18-19 0.38827081239110584 1105 20-21 0.1700416235207456 1105 22-23 0.12972500976167822 1105 24-25 0.30285587383818324 1105 26-27 0.3006932951876422 1105 28-29 0.2763567568718983 1105 30-31 0.2620112254814799 1105 32-33 0.12907564553190554 1105 34-35 0.1900734390423935 1105 36-37 0.33874678029015115 1105 38-39 0.1513831350899082 1105 40-41 0.05989826879849858 1105 42-43 0.11428716628090996 1105 44-45 0.17202660970158234 1105 46-47 0.19961291969845263 1105 48-49 0.2574302759463052 1105 50-51 -0.025588004002955245 1105 52-53 0.2977589792458275 1105 54-55 -0.11971623689568389 1105 56-57 0.3488393782629089 1105 58-59 0.4007794065629824 1105 60-61 0.19020278311069916 1105 62-63 0.3905056093424477 1105 64-65 0.3913446001253291 1105 66-67 -0.025665690016772658 1105 68-69 0.20041332976479254 1105 70-71 0.3132427585415982 1105 72-73 0.5207193446337612 1105 74-75 0.36607929123272953 1105 76 0.20371975238624884 1106 1 0.23825949573985383 1106 2 0.06926014724164986 1106 3 0.0398104094243692 1106 4 0.224745433007719 1106 5 0.19696912956102608 1106 6 0.11063596220546401 1106 7 0.11153353411430089 1106 8 0.24533669472145903 1106 9 0.1888345770267108 1106 10-11 0.21777838693649443 1106 12-13 0.3007940613554254 1106 14-15 0.20122279928670395 1106 16-17 0.3056897704494119 1106 18-19 0.31394056747262766 1106 20-21 0.2769438715994639 1106 22-23 0.0912131947792787 1106 24-25 0.42969874773988437 1106 26-27 0.2715640366466161 1106 28-29 0.4526908802648464 1106 30-31 0.3475890463534199 1106 32-33 0.4470650635213218 1106 34-35 0.24029728926624472 1106 36-37 0.4499129561622084 1106 38-39 0.1988034707455384 1106 40-41 0.5601711984831965 1106 42-43 0.3704436459668017 1106 44-45 0.446919472829741 1106 46-47 0.4160078537404388 1106 48-49 0.5082143502597916 1106 50-51 0.5196752294955687 1106 52-53 0.5591035170609544 1106 54-55 0.45923226558223007 1106 56-57 0.6359829536417791 1106 58-59 0.5765916117869523 1106 60-61 0.5337661913799892 1106 62-63 0.41771238949040423 1106 64-65 0.6127863392141251 1106 66-67 0.515458109930556 1106 68-69 0.43729714311919565 1106 70-71 0.773976795746222 1106 72-73 0.9254333669948451 1106 74-75 0.8223028062797724 1106 76 1.1915719872973085 1107 1 0.008247990513538639 1107 2 -0.005984032827377916 1107 3 -0.18565285952865196 1107 4 0.13762394271372358 1107 5 -0.016954642194868086 1107 6 0.22164426065592835 1107 7 0.36555424352165744 1107 8 0.43424271905378475 1107 9 0.19395073085403425 1107 10-11 0.291186626636879 1107 12-13 0.13887880836283983 1107 14-15 0.3103584385611313 1107 16-17 0.31625254737041786 1107 18-19 0.1580325113662937 1107 20-21 0.30561391639641045 1107 22-23 0.1942021056994534 1107 24-25 0.2785812720354883 1107 26-27 0.21232436075128902 1107 28-29 0.5694052813811012 1107 30-31 0.27448190087350355 1107 32-33 0.36920405644683285 1107 34-35 0.11337261177977354 1107 36-37 0.2796160234065823 1107 38-39 0.14539474146106812 1107 40-41 0.26368886022954285 1107 42-43 0.4166701765290881 1107 44-45 0.3508141143317829 1107 46-47 0.16295407292724207 1107 48-49 0.28040842139635913 1107 50-51 0.46106466916656785 1107 52-53 0.5513705362281698 1107 54-55 0.5497367819955414 1107 56-57 0.36738487770728767 1107 58-59 0.5165220908449939 1107 60-61 0.08236230897809094 1107 62-63 0.34956228300585934 1107 64-65 0.46302011562107737 1107 66-67 0.6699757471976966 1107 68-69 0.5019320357778838 1107 70-71 0.5959566738397797 1107 72-73 0.048451163376817874 1107 74-75 0.23682876647658446 1107 76 0.20695471966615742 1108 1 -0.2949175959332422 1108 2 0.05544586131941287 1108 3 0.1175527659561908 1108 4 0.059347623302301145 1108 5 0.04648220873478692 1108 6 -0.2549404093973706 1108 7 -0.13721095254450688 1108 8 0.13089695693572168 1108 9 0.005692178765748679 1108 10-11 -0.03314960118525079 1108 12-13 -0.27103585843728695 1108 14-15 -0.05336619295459144 1108 16-17 0.08122339394583378 1108 18-19 0.19879725699328077 1108 20-21 0.17426835447353284 1108 22-23 0.057376200441829894 1108 24-25 0.25033623312255315 1108 26-27 0.19446945221546486 1108 28-29 0.28603149193639155 1108 30-31 0.1769993553916649 1108 32-33 0.2600651460091612 1108 34-35 -0.05954598385097398 1108 36-37 0.23342849056206205 1108 38-39 0.07031654021484712 1108 40-41 0.03275369844680398 1108 42-43 0.07338797638394823 1108 44-45 0.1413724086687651 1108 46-47 0.4334928482331648 1108 48-49 0.36842173437651127 1108 50-51 0.18117427603326774 1108 52-53 0.08465048417741627 1108 54-55 0.06880036899286068 1108 56-57 0.18191654608394714 1108 58-59 0.14510746158146048 1108 60-61 0.34433773501847753 1108 62-63 0.18620300771245724 1108 64-65 0.4571293721461842 1108 66-67 0.4263081713112058 1108 68-69 0.09998013267235706 1108 70-71 0.6501795411353939 1108 72-73 0.39177590499989634 1108 74-75 0.29122430212384387 1108 76 0.6221245102119397 1109 1 -0.0895309486032616 1109 2 -0.2965249264777583 1109 3 0.0910390662617857 1109 4 -0.08377663214908893 1109 5 0.12825047931022482 1109 6 -0.26568262724582326 1109 7 -0.10123436374251327 1109 8 -0.03259814846646236 1109 9 0.03285849150206133 1109 10-11 -0.051601458024741476 1109 12-13 0.08060862283893044 1109 14-15 0.14040808580440256 1109 16-17 0.10748588941379467 1109 18-19 0.2489914870652683 1109 20-21 0.2626559859025477 1109 22-23 -0.1348545966783874 1109 24-25 0.06679658429052182 1109 26-27 0.036599249698241465 1109 28-29 -0.09686072332232243 1109 30-31 -0.11270127132403474 1109 32-33 -0.11710375646145366 1109 34-35 -0.250228119863813 1109 36-37 -0.10319035234466156 1109 38-39 -0.1693426507521565 1109 40-41 -0.15529467778979722 1109 42-43 -0.04989632464676674 1109 44-45 -0.05959874169121093 1109 46-47 0.025117647528759335 1109 48-49 -0.29416696402302733 1109 50-51 -0.07698079725621909 1109 52-53 0.13064934469433354 1109 54-55 -0.25688720546200017 1109 56-57 -0.012481858639723953 1109 58-59 0.16534623508329815 1109 60-61 -0.007407745895179119 1109 62-63 -0.22356288030743343 1109 64-65 0.004109741960235169 1109 66-67 -0.2433375530374775 1109 68-69 -0.2927504831664578 1109 70-71 0.1289851556793451 1109 72-73 -0.43776519408333314 1109 74-75 -0.10436538037473753 1109 76 -0.11000373343026126 1110 1 0.027439213748650104 1110 2 0.13008762288599485 1110 3 0.08031067661720215 1110 4 0.10110708878114139 1110 5 0.028360176545859872 1110 6 -0.10989061320457694 1110 7 0.026859098302239204 1110 8 -0.09037568247260452 1110 9 0.27890939101939694 1110 10-11 0.017699770584300722 1110 12-13 0.16133481853879772 1110 14-15 -0.2087691849283715 1110 16-17 0.13470177357351076 1110 18-19 0.09944124573134872 1110 20-21 0.08683874149711102 1110 22-23 -0.151534061355882 1110 24-25 0.09982093707682793 1110 26-27 0.04301653798258087 1110 28-29 0.007159680363507448 1110 30-31 -0.10189609361451346 1110 32-33 0.10185192585535674 1110 34-35 0.2315621390216549 1110 36-37 0.4382258389673197 1110 38-39 0.10347985912058988 1110 40-41 0.016924497286986195 1110 42-43 0.1403186819350708 1110 44-45 0.25489774799724785 1110 46-47 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3 -0.01816833763000858 2116 4 -0.007493494309237292 2116 5 -0.14179494749836863 2116 6 0.16816373521943007 2116 7 0.13788693261655993 2116 8 0.07610074637386788 2116 9 -0.05687640577910713 2116 10-11 0.030217648355353788 2116 12-13 0.03776504022315663 2116 14-15 0.18780790952127546 2116 16-17 -0.3835541810994556 2116 18-19 -0.32516269287913246 2116 20-21 -0.20724027825094993 2116 22-23 -0.10510342710757214 2116 24-25 -0.13645787363882533 2116 26-27 0.021886387809303187 2116 28-29 -0.07129966833554846 2116 30-31 -0.07719038923033139 2116 32-33 -0.20124505417654603 2116 34-35 -0.09848901890782003 2116 36-37 -0.0729374538430605 2116 38-39 -0.11522372743532117 2116 40-41 -0.2710279478420077 2116 42-43 -0.3015235536114389 2116 44-45 0.0016969393719108439 2116 46-47 -0.10245032318111669 2116 48-49 -0.25299807121673723 2116 50-51 -0.19387685800609944 2116 52-53 -0.25413606448594805 2116 54-55 -0.13617732682615724 2116 56-57 -0.1566102741255193 2116 58-59 -0.2917525512750956 2116 60-61 -0.37824305676371495 2116 62-63 0.024145438900646354 2116 64-65 -0.14500884235056333 2116 66-67 -0.030106147518459636 2116 68-69 0.1597988422115364 2116 70-71 -0.11490256552209388 2116 72-73 -0.1582445486158477 2116 74-75 0.3678734154758345 2116 76 -0.01749576678845699 2117 1 -0.08865255563040364 2117 2 -0.02533909596440509 2117 3 -0.2916882719195115 2117 4 -0.5807814561105005 2117 5 -0.20344366713660378 2117 6 -0.04278681041235899 2117 7 -0.2919752378155209 2117 8 -0.7819537536216217 2117 9 -0.2871429484864194 2117 10-11 -0.22701005475597213 2117 12-13 -0.268005388248973 2117 14-15 0.0612159193417412 2117 16-17 -0.10756559017436729 2117 18-19 -0.41860157219025496 2117 20-21 -0.4003867097558853 2117 22-23 -0.1122611774257436 2117 24-25 -0.7259026741154102 2117 26-27 -0.4803566146548377 2117 28-29 -1.6692561441589575 2117 30-31 -0.7526861514449976 2117 32-33 -0.49502873306163764 2117 34-35 -0.20695646603704887 2117 36-37 -0.420801411456182 2117 38-39 0.1164734627189361 2117 40-41 -0.182002864124307 2117 42-43 -0.2881964182460166 2117 44-45 -0.5347913401504272 2117 46-47 -0.4543625166299279 2117 48-49 -0.6191219643830266 2117 50-51 -0.16057932846796774 2117 52-53 -0.3696890525984884 2117 54-55 -0.28947774473768106 2117 56-57 -0.5346396813771364 2117 58-59 -0.40580719568925616 2117 60-61 -0.3824131458519773 2117 62-63 -0.48287240583123037 2117 64-65 -0.42499602519362156 2117 66-67 -0.48201924358395587 2117 68-69 -0.01809788038728044 2117 70-71 0.22426919340704643 2117 72-73 -0.07278931389037524 2117 74-75 0.40813011966126567 2117 76 -0.8458090469877533 2118 1 -0.21671996062136856 2118 2 -0.32859922021897603 2118 3 -0.5266603250289918 2118 4 -0.2160070051785894 2118 5 -0.3978459776899257 2118 6 -0.36223327497486935 2118 7 -0.26950344443550733 2118 8 -0.42168820621579783 2118 9 -0.3457898618614479 2118 10-11 -0.4024676980746804 2118 12-13 -0.22974723793834784 2118 14-15 -0.2006173551195829 2118 16-17 -0.17818603192224458 2118 18-19 -0.4959905638165836 2118 20-21 -0.27936836246642116 2118 22-23 -0.26601582658317824 2118 24-25 -0.109206225136667 2118 26-27 -0.5192381156833648 2118 28-29 -0.0773898321095885 2118 30-31 -0.2040076549669294 2118 32-33 -0.27173931132332285 2118 34-35 -0.8353303517976371 2118 36-37 -0.5500687665790878 2118 38-39 -0.10428099943329272 2118 40-41 -0.2040850633056408 2118 42-43 -0.33907222600466014 2118 44-45 -0.6447556013759268 2118 46-47 -0.430477794956019 2118 48-49 -0.47852001537311395 2118 50-51 -0.48431652380529755 2118 52-53 -0.7372841655288624 2118 54-55 -0.1677581278905933 2118 56-57 -0.5999252798977253 2118 58-59 -0.9021190372750567 2118 60-61 -0.6283457824177319 2118 62-63 -0.41872442237080776 2118 64-65 -0.4230480542002084 2118 66-67 -0.4132532702218086 2118 68-69 -1.0013972757370837 2118 70-71 -0.4450432507511195 2118 72-73 -0.6247559854053222 2118 74-75 -0.5003931624880451 2118 76 -0.903323539891943 2119 1 -0.2563548224038783 2119 2 -0.26782107219251117 2119 3 0.006521258488163539 2119 4 -0.2537880161139654 2119 5 -0.14616812534093526 2119 6 -0.6596003374426047 2119 7 -0.08906978413607192 2119 8 -0.46596942834936783 2119 9 -0.0866731396574778 2119 10-11 -0.2746458554877407 2119 12-13 -0.4317348476440728 2119 14-15 -0.9670321483800137 2119 16-17 -0.48185384062361436 2119 18-19 -0.09129961717932389 2119 20-21 -0.1520862486071266 2119 22-23 -0.37167359163692026 2119 24-25 -0.7879033669210571 2119 26-27 -0.3941618496675119 2119 28-29 -0.09332356280842191 2119 30-31 -0.20552124205098465 2119 32-33 -0.3492959828397275 2119 34-35 -0.3037327547638 2119 36-37 -0.29217230062892696 2119 38-39 -0.25765782395105674 2119 40-41 -0.06437745547722784 2119 42-43 -0.5039945520008047 2119 44-45 -0.2280732903983207 2119 46-47 -0.3449425248569895 2119 48-49 -0.31824404972802256 2119 50-51 -0.607855736270686 2119 52-53 -0.628165096678245 2119 54-55 -0.3960399132193544 2119 56-57 -0.3439178644943368 2119 58-59 -0.1406791348955636 2119 60-61 -0.299357656449736 2119 62-63 -0.305698186861342 2119 64-65 -1.0496144408337074 2119 66-67 -0.7778634922145784 2119 68-69 -0.6381481087966492 2119 70-71 -0.24584815054931397 2119 72-73 -0.39411582020139946 2119 74-75 -0.40115347600004014 2119 76 -1.705970557304063 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 3.0 21 3.0 22 12.0 23 24.0 24 62.0 25 95.0 26 179.0 27 364.0 28 601.0 29 997.0 30 1567.0 31 2327.0 32 3840.0 33 6047.0 34 9143.0 35 13708.0 36 24072.0 37 89760.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.52036909786983 20.079185890514054 13.083995942541145 29.316449069074963 2 19.67867543601322 20.935178822682506 34.58067471614149 24.805471025162788 3 19.186544942901083 21.82127548182324 30.55266516148032 28.439514413795358 4 14.979221884100651 21.143287196099603 34.8640424069893 29.01344851281045 5 14.937338437878342 33.07941494061058 36.555086548215044 15.428160073296032 6 28.966329635810347 36.805732796701676 17.882268250384477 16.345669317103496 7 27.80406400314126 27.56716076044632 22.830404764242008 21.798370472170415 8 24.54893491705114 34.41379536009948 18.96142141945617 22.075848303393215 9 23.328425116979158 16.81358594286836 20.996695134321524 38.86129380583096 10-11 26.228199339026865 25.97526258957495 25.268479434573475 22.52805863682471 12-13 25.348974182781976 21.70609600471189 25.613036222636694 27.331893589869445 14-15 22.824842119040607 22.078138804358495 22.523150420470532 32.57386865613036 16-17 25.0721507804064 26.425182422041164 23.23418736297896 25.268479434573475 18-19 25.46938909067112 25.613690651483918 24.276692516606133 24.640227741238835 20-21 23.574490363535226 25.301528091358268 23.945551519911 27.17843002519551 22-23 24.226301495369913 25.984424593436078 23.657275612709007 26.131998298484998 24-25 25.690258826609075 26.70855011288897 23.537842348090702 24.06334871241124 26-27 24.87713098393377 27.377703609175093 24.143189031772522 23.601976375118618 28-29 25.402964562677923 24.903308137822716 24.625175877752692 25.068551421746673 30-31 25.128758875691243 26.40064134027028 24.886620202218516 23.583979581819968 32-33 23.40532050652793 26.267792284283892 24.813324171329473 25.51356303785871 34-35 24.314976604168713 24.885311344524066 25.37678740878898 25.42292464251824 36-37 24.797290664572493 25.254736428781783 26.524328392395535 23.42364451425019 38-39 22.304243971074243 25.923889925067893 27.630640358627005 24.141225745230848 40-41 23.26101894571513 23.390595857465396 27.32305880043192 26.025326396387555 42-43 24.828048820392006 23.508393049965644 27.00271587971598 24.660842249926375 44-45 22.262033310428322 25.505709891692025 28.36818166944799 23.86407512843166 46-47 23.61015673570891 24.00804947482085 27.728804685710546 24.652989103759694 48-49 23.39484964497235 23.280324596708223 26.529891037596936 26.79493472072249 50-51 22.68446713131115 23.713229279146624 27.30048100520271 26.30182258433952 52-53 20.904093452439383 23.673636333889597 30.922417460161643 24.499852753509373 54-55 21.246686953960932 24.431137724550897 28.66791008147639 25.65426524001178 56-57 22.184156277608714 25.180785969045516 26.582245345374822 26.052812407970944 58-59 21.13674290762737 24.98445731487844 28.70259481037924 25.176204967114952 60-61 22.283956676810313 26.606459212722093 26.65161480318052 24.457969307287065 62-63 20.53859494126501 29.370112234547296 25.09211086024672 24.99918196394097 64-65 20.435195183403685 30.391021236216094 24.059749353751513 25.11403422662871 66-67 20.634795981806878 29.53830044828376 23.966493243022153 25.860410326887205 68-69 20.231667811917152 30.057589738555674 24.20895913091849 25.501783318608684 70-71 20.984915415071494 29.055004744609143 24.05353227970289 25.90654756061647 72-73 21.11841889990511 27.938549131245704 24.03913484506397 26.903897123785214 74-75 21.070645594057787 27.905500474460915 24.183436405876773 26.840417525604526 76 21.349432282975034 27.94869277837767 23.85458591014692 26.847289028500377 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 3.0 14 6.0 15 5.5 16 14.5 17 25.0 18 40.0 19 57.0 20 69.5 21 80.0 22 98.0 23 169.5 24 256.5 25 290.0 26 420.5 27 610.0 28 824.5 29 980.0 30 1092.0 31 1439.5 32 1958.0 33 2241.0 34 2546.0 35 3132.5 36 3655.5 37 3897.0 38 4380.5 39 5627.5 40 6998.5 41 8440.5 42 9275.0 43 10036.5 44 10972.0 45 10589.5 46 10033.0 47 9723.5 48 8868.5 49 7802.0 50 7281.0 51 6670.5 52 5393.5 53 4338.0 54 3949.0 55 3736.0 56 3109.0 57 2482.0 58 2269.0 59 2013.5 60 1692.5 61 1630.0 62 1633.0 63 1659.5 64 1774.0 65 1924.0 66 2067.5 67 2149.0 68 2213.5 69 2231.0 70 2162.0 71 2140.0 72 2044.5 73 1777.0 74 1444.5 75 1284.0 76 1070.0 77 715.0 78 432.5 79 291.0 80 224.0 81 110.0 82 40.5 83 18.0 84 9.5 85 1.0 86 1.5 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 152805.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.40433886325709 #Duplication Level Percentage of deduplicated Percentage of total 1 63.9451782160304 27.75498183959949 2 17.69193655388698 15.358136186643106 3 6.522525782522163 8.493177579267694 4 3.0335926663048065 5.266843362455417 5 1.7670828056208916 3.834953044730212 6 1.402207345757192 3.651712967507608 7 1.0569326337374103 3.2112823533261343 8 0.817200410107955 2.8376034815614672 9 0.5804836861467946 2.267595955629724 >10 3.142150654363428 21.256503386669284 >50 0.028647246848802845 0.7604463204738064 >100 0.006030999336590073 0.5209253623899741 >500 0.0030154996682950365 1.177971925002454 >1k 0.0030154996682950365 3.607866234743627 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT 3692 2.416151303949478 No Hit CACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTTCTG 1821 1.1917149307941495 TruSeq Adapter, Index 2 (95% over 24bp) TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG 924 0.6046922548345931 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC 876 0.5732796701678611 No Hit TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC 309 0.20221851379208797 No Hit ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT 214 0.14004777330584733 TruSeq Adapter, Index 2 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005235430777788685 0.0 2 0.0 0.0 0.0 0.00588985962501227 0.0 3 0.0 0.0 0.0 0.00588985962501227 0.0 4 0.0 0.0 0.0 0.019632865416707567 0.0 5 0.0 0.0 0.0 0.020287294263931156 0.0 6 0.0 0.0 0.0 0.020287294263931156 0.0 7 0.0 0.0 0.0 0.020287294263931156 0.0 8 0.0 0.0 0.0 0.021596151958378326 0.0 9 0.0 0.0 0.0 0.02355943850004908 0.0 10 0.0 0.0 0.0 0.02421386734727267 0.0 11 0.0 0.0 0.0 0.02421386734727267 0.0 12 0.0 0.0 0.0 0.024868296194496254 0.0 13 0.0 0.0 0.0 0.02552272504171984 0.0 14 0.0 0.0 0.0 0.02552272504171984 0.0 15 0.0 0.0 0.0 0.026177153888943424 0.0 16 0.0 0.0 0.0 0.02683158273616701 0.0 17 0.0 0.0 0.0 0.027486011583390597 0.0 18 0.0 0.0 0.0 0.028140440430614182 0.0 19 0.0 0.0 0.0 0.029449298125061352 0.0 20 0.0 0.0 0.0 0.030103726972284937 0.0 21 0.0 0.0 0.0 0.03272144236117928 0.0 22 0.0 0.0 0.0 0.035993586597297206 0.0 23 0.0 0.0 0.0 0.037956873138967964 0.0 24 0.0 0.0 0.0 0.04122901737508589 0.0 25 0.0 0.0 0.0 0.04319230391675665 0.0 26 0.0 0.0 0.0 0.04908216354176892 0.0 27 0.0 0.0 0.0 0.07984031936127745 0.0 28 0.0 0.0 0.0 0.11845162134746899 0.0 29 0.0 0.0 0.0 0.1596806387225549 0.0 30 0.0 0.0 0.0 0.2002552272504172 0.0 31 0.0 0.0 0.0 0.2454108177088446 0.0 32 0.0 0.0 0.0 0.3043094139589673 0.0 33 0.0 0.0 0.0 0.3776054448480089 0.0 34 0.0 0.0 0.0 0.4914760642649128 0.0 35 0.0 0.0 0.0 0.6413402702791139 0.0 36 0.0 0.0 0.0 0.833742351362848 0.0 37 0.0 0.0 0.0 1.1341251922384739 0.0 38 0.0 0.0 0.0 1.5385622198226498 0.0 39 0.0 0.0 0.0 1.9050423742678577 0.0 40 0.0 0.0 0.0 2.3219135499492816 0.0 41 0.0 0.0 0.0 2.8408756257975853 0.0 42 0.0 0.0 0.0 3.4324793036877064 0.0 43 0.0 0.0 0.0 4.036517129675076 0.0 44 0.0 0.0 0.0 4.613068944079055 0.0 45 0.0 0.0 0.0 5.16344360459409 0.0 46 0.0 0.0 0.0 5.76748143058146 0.0 47 0.0 0.0 0.0 6.337488956513203 0.0 48 0.0 0.0 0.0 6.861032034292071 0.0 49 0.0 0.0 0.0 7.412715552501554 0.0 50 0.0 0.0 0.0 7.963744641863813 0.0 51 0.0 0.0 0.0 8.554039462059487 0.0 52 0.0 0.0 0.0 9.193416445796931 0.0 53 0.0 0.0 0.0 9.863551585353882 0.0 54 0.0 0.0 0.0 10.472170413271817 0.0 55 0.0 0.0 0.0 10.980007198717319 0.0 56 0.0 6.544288472235856E-4 0.0 11.502241418801741 0.0 57 0.0 6.544288472235856E-4 0.0 12.038873073525082 0.0 58 0.0 6.544288472235856E-4 0.0 12.54801871666503 0.0 59 0.0 6.544288472235856E-4 0.0 13.060436504041098 0.0 60 0.0 6.544288472235856E-4 0.0 13.614083308792251 0.0 61 0.0 6.544288472235856E-4 0.0 14.1925984097379 0.0 62 0.0 6.544288472235856E-4 0.0 14.73970092601682 0.0 63 0.0 6.544288472235856E-4 0.0 15.325414744281927 0.0 64 0.0 6.544288472235856E-4 0.0 15.888223552894212 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCATA 20 0.006940563 52.500004 40 TTAATAC 20 0.006940563 52.500004 3 GTCTCTC 20 0.006940563 52.500004 67 GAGAAAC 20 0.006940563 52.500004 19 CGCTAAT 20 0.006940563 52.500004 48 AAGGCAT 20 0.006940563 52.500004 57 ATCGCAT 20 0.006940563 52.500004 39 GTGCTAA 30 5.863326E-4 46.666664 1 CTAATGG 30 5.863326E-4 46.666664 4 CGAATTC 35 0.0012523554 40.0 41 TCTTTAT 80 6.1907485E-8 35.000004 1 CTTTATA 90 4.454705E-9 35.000004 2 TAGAAGC 50 1.7035654E-4 35.0 59 AAGCCGG 60 1.2096694E-5 35.0 62 GCGTAAA 50 1.7035654E-4 35.0 68 GGCGTAA 55 2.9814086E-4 31.81818 67 GACAGAC 45 0.0042968923 31.111113 30 CTAACTT 45 0.0042968923 31.111113 21 TGACAGA 45 0.0042968923 31.111113 29 AAAATTG 45 0.0042968923 31.111113 61 >>END_MODULE