FastQCFastQC Report
Wed 25 May 2016
SRR1780351_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780351_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520983
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGTAT300.005730285329.3456424
GTTTCGT602.8727867E-421.99654211
CGTGCGA500.00258083721.9965410
CGTTTCG703.2147185E-521.99653810
ACCCTTG500.002586602521.9880925
TCTAGCA755.5162716E-520.508433
CCATATG654.967464E-420.2830628
CGAAACT550.004451995720.02186434
CGTTTAC550.004461921320.01416214
CAATGTG550.004504306219.98149732
TAATATG600.0073559518.3551436
ATTGTAG600.007388672318.34102424
GGCCATT600.00745447518.31285744
CTACACG1102.8227496E-617.978174
TTCGGTC750.001294221117.59047128
CCTTGTA750.001300066217.5786552
AAGCTGC750.001300066217.5786557
CTAGCCT1154.313044E-617.1965084
CCGTTTC902.2329285E-417.0903599
CTCTAGC1053.8070997E-516.7415772
Produced by FastQC (version 0.11.5)