FastQCFastQC Report
Wed 25 May 2016
SRR1780351_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780351_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520983
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGT135862.6077626333296866No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC43740.8395667421009898No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG36430.6992550620653649No Hit
CACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCTTCTG25540.49022712833240234RNA PCR Primer, Index 31 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC7790.14952503248666463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCTG3350.036.12113644
ATCAGTA250.002351014635.20168739
TAACCGC250.002352122235.1983117
ATGCCGT18750.033.3242744
TTCGGTA401.8186916E-532.99841726
GTCTTCT3700.032.70427343
CCGTCTT3750.032.26821541
CGTCTTC3850.031.43007942
TCGGTAA502.358931E-630.79852127
CTTATAA300.005703096329.3742141
CGGTAAG454.066482E-529.33192828
CTTAAGA300.005743398329.3319243
GCCGTCT4250.028.98962640
GATCAGT407.024752E-427.5013238
CTTTATA1650.026.6653882
TATGCCG23350.026.66509243
TGCCGTC4750.026.40126839
TCTGTCC508.329768E-526.40126638
GGTTCCA450.001385854424.4785141
TCACGCT450.001396756224.44561841
Produced by FastQC (version 0.11.5)