FastQCFastQC Report
Wed 25 May 2016
SRR1780350_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780350_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35650
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTAGT150.002207214570.046
CGCTACG150.002207214570.016
AGGTCAA200.006897184552.514
CGTTCGG200.006897184552.524
GTCCAAT200.006897184552.51
GTCTCCA400.0023882335.011
AGTCCGG703.3978857E-530.00000270
CAGTCCG703.3978857E-530.00000269
GCAGTCC808.441722E-526.2568
AGCAGTC1003.8315132E-421.067
GAGCAGT1055.3252524E-420.066
ACGAGCA1400.003659766215.00000164
CGAGCAG1550.007191855513.54838765
TGCCGAC3250.0096819378.61538470
Produced by FastQC (version 0.11.5)