FastQCFastQC Report
Wed 25 May 2016
SRR1780350_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780350_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35650
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT35029.82328190743338No Hit
CACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCTG17664.953716690042076RNA PCR Primer, Index 23 (95% over 24bp)
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG8552.3983169705469845No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC6921.9410939691444602No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC3300.9256661991584854No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT1660.46563814866760167RNA PCR Primer, Index 47 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT1630.45722300140252453No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT1220.34221598877980364No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCGT1040.2917251051893408No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG890.2496493688639551No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC810.22720897615708274No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG740.20757363253856945No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC500.1402524544179523No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC480.13464235624123422No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAGCTCGTATGCCGT470.13183730715287517No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCG430.12061711079943899No Hit
TATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCT380.10659186535764377No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCG380.10659186535764377No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTATA401.2914825E-1070.02
ATAGTAG150.002207214570.070
TCTTTAT401.2914825E-1070.01
GCTTATA150.002207214570.03
TTTATAC553.45608E-1157.2727243
AGCTTAT200.006897184552.52
CTTTACA200.006897184552.51
TTGAACA200.006897184552.559
GCGTACG200.006897184552.556
ACCAGCT200.006897184552.550
CAGCTTA200.006897184552.51
CCGCGTA305.80266E-446.66666454
CCTCGTA501.6775295E-435.038
GGTAGAC601.1838774E-535.032
GTAGACC552.9360698E-431.8181833
TAGACCT604.889215E-429.16666634
CTTGAAA10350.029.08212757
TTGAAAA10400.028.9423158
TGAAAAA10400.028.9423159
TGCTTGA10450.028.8038355
Produced by FastQC (version 0.11.5)