FastQCFastQC Report
Wed 25 May 2016
SRR1780349_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780349_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333121
Sequences flagged as poor quality0
Sequence length50
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGAAG250.002353425135.1906211
TCAGAAC250.00236559735.1536453
GTATAGA401.830701E-532.961491
CTATAGT300.00577195529.2991031
TATAGAG300.00577195529.2991032
ACGAGAG651.8056906E-523.69667824
AGAGGCT500.002585042521.98753227
CTTTACA602.889455E-421.9743271
TTGGGGA602.892391E-421.971033
CGAAACT755.4812488E-520.52169825
GAACTGT654.89581E-420.32365834
CTACACA550.004511180319.9736634
ATTTGCG809.002768E-519.2390930
TTTACAC951.6075399E-518.5046982
TTACACA951.609621E-518.501923
AAATTTG851.4330998E-418.1073828
ACGAAAC1002.470334E-517.60324724
GAAAATT902.2188936E-417.10141426
TACACCG800.002001833216.4782715
AAAATTT1105.6288663E-515.990931527
Produced by FastQC (version 0.11.5)