FastQCFastQC Report
Wed 25 May 2016
SRR1780349_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780349_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333121
Sequences flagged as poor quality0
Sequence length50
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGT231136.938319709655049No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC61881.8575832805497101No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG60931.8290651144779224No Hit
CACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTTCTG32800.9846272075311974Illumina PCR Primer Index 2 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC11950.3587285100609088No Hit
ATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTT3860.11587381161800067Illumina PCR Primer Index 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTCCATATCTCGTATGCCGTCT3780.11347228184353433No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA207.810113E-444.0437281
TCTTTAT950.041.725631
TCTTCTG4250.036.23863244
GCTTTGA250.002351023735.1979615
GTCTTCT4400.034.50317843
CTTTATA1150.034.4379542
GCTTATA451.0334752E-634.2253763
ATGCCGT29600.034.11800844
CCGTCTT4600.033.00303641
TTTATAC1400.031.4314653
CGTCTTC5050.030.9335442
CAGCTTA502.3364992E-630.8306061
TATATAC300.00573653329.3360354
AGAATGG300.00574074429.331635
AGCTTAT554.943353E-628.002582
TATGCCG36350.027.78247843
GCCGTCT6050.025.0932240
CTTAATA651.794427E-523.7158531
TAATACA651.8069913E-523.6944893
TTAATAC651.8069913E-523.6944892
Produced by FastQC (version 0.11.5)