FastQCFastQC Report
Wed 25 May 2016
SRR1780348_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780348_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51519
Sequences flagged as poor quality0
Sequence length76
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTAA207.8570425E-570.01
ACTCTGT252.3695813E-456.0000048
CGAACAT200.006914580252.525
CCGATTA200.006914580252.517
CTGAGCA200.006914580252.52
CGATTAC200.006914580252.518
TTTCAGC200.006914580252.530
AAGCAGC200.006914580252.557
CGCTACA200.006914580252.517
TCCCGCA200.006914580252.513
GAATTTA200.006914580252.526
TTAGATC350.001244635140.038
GAACATA350.001244635140.026
CGACGAA654.7507092E-737.6923122
TTGTAGA400.002398111935.036
GCCGACT400.002398111935.022
CCGACTA400.002398111935.023
TAGATCT1357.2759576E-1231.1111139
GTTCTTC450.00427072331.11110952
CTCTAAT450.00427072331.1111091
Produced by FastQC (version 0.11.5)