##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780348_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 51519 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.424406529629845 27.0 12.0 34.0 12.0 34.0 2 24.388982705409656 27.0 12.0 34.0 12.0 34.0 3 23.475669170597257 23.0 11.0 34.0 11.0 34.0 4 24.10448572371358 27.0 11.0 34.0 11.0 34.0 5 23.976843494633048 27.0 11.0 34.0 11.0 34.0 6 25.290844154583745 27.0 11.0 37.0 11.0 38.0 7 25.638696403268696 27.0 12.0 37.0 12.0 38.0 8 25.686523418544613 27.0 11.0 37.0 11.0 38.0 9 25.676333003357986 27.0 12.0 37.0 11.0 38.0 10-11 25.226207806828548 26.5 11.0 37.0 11.0 38.0 12-13 25.3250257186669 27.0 11.0 37.0 11.0 38.0 14-15 24.729759894407888 26.0 11.0 37.0 11.0 38.0 16-17 24.6591451697432 26.0 11.0 37.0 11.0 38.0 18-19 24.80678002290417 26.0 11.0 37.0 11.0 38.0 20-21 24.821551272346127 26.0 11.0 37.0 11.0 38.0 22-23 24.657272074380327 26.0 11.0 37.0 11.0 38.0 24-25 24.626662008191154 26.0 11.0 37.0 11.0 38.0 26-27 24.398746093674177 26.0 11.0 37.0 11.0 38.0 28-29 24.269851899299287 26.0 11.0 37.0 11.0 38.0 30-31 24.30422756652885 26.0 11.0 37.0 11.0 38.0 32-33 24.39407791300297 26.0 11.0 37.0 11.0 38.0 34-35 24.426396086880565 26.0 11.0 37.0 11.0 38.0 36-37 24.410838719695647 26.0 11.0 37.0 11.0 38.0 38-39 24.165987305654223 26.0 11.0 37.0 11.0 38.0 40-41 24.345474485141402 26.0 11.0 37.0 11.0 38.0 42-43 24.51649876744502 26.0 11.0 37.0 11.0 38.0 44-45 24.628777732487045 26.0 11.0 37.0 11.0 38.0 46-47 24.263019468545586 26.0 11.0 37.0 11.0 38.0 48-49 24.102612628350705 25.5 11.0 37.0 11.0 38.0 50-51 24.07637958811312 26.0 11.0 37.0 11.0 38.0 52-53 24.23964944971758 26.0 11.0 37.0 11.0 38.0 54-55 24.197199091597277 26.0 11.0 37.0 11.0 38.0 56-57 24.049467186863097 25.0 11.0 37.0 11.0 38.0 58-59 24.071711407441914 25.0 11.0 37.0 11.0 38.0 60-61 24.222607193462608 25.0 11.0 37.0 11.0 38.0 62-63 24.00598808206681 24.5 11.0 37.0 11.0 38.0 64-65 23.961635513111666 24.5 11.0 37.0 11.0 38.0 66-67 24.13111667540131 25.0 11.0 37.0 11.0 38.0 68-69 23.864438362545854 24.5 11.0 37.0 11.0 38.0 70-71 23.73118655253402 24.0 11.0 37.0 11.0 38.0 72-73 23.676235951784776 23.5 11.0 37.0 11.0 38.0 74-75 23.505095207593314 23.5 11.0 37.0 11.0 38.0 76 23.306721791960246 23.0 11.0 36.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7678070181049108 1101 2 0.2249837254924465 1101 3 -0.2240396744033042 1101 4 -0.8925645016965191 1101 5 -0.10866733885682933 1101 6 -0.07353114133829664 1101 7 0.6230607949576488 1101 8 -0.3556296656325415 1101 9 1.335612594485923 1101 10-11 0.07003560344524828 1101 12-13 0.2922047437631825 1101 14-15 1.1657259840692227 1101 16-17 0.9650797717622908 1101 18-19 0.8320151428387348 1101 20-21 0.3666556106285057 1101 22-23 0.1485062230525962 1101 24-25 -0.41328235438959027 1101 26-27 0.49270562834989207 1101 28-29 -0.09000995907521059 1101 30-31 0.34195446321707834 1101 32-33 0.3834223525843825 1101 34-35 0.5713162876551863 1101 36-37 0.16643496130552293 1101 38-39 0.7058068498265442 1101 40-41 0.8591672750651931 1101 42-43 1.005312533150974 1101 44-45 0.22681449867285863 1101 46-47 0.38854278578513757 1101 48-49 0.6971630351259606 1101 50-51 0.5313214192807507 1101 52-53 1.1935205480490794 1101 54-55 0.2565562658373466 1101 56-57 0.13724796363342406 1101 58-59 -0.0010508154590738172 1101 60-61 0.19425618440375203 1101 62-63 -0.16397660974399741 1101 64-65 -0.013880513091411473 1101 66-67 0.148800458894204 1101 68-69 0.10101657882787762 1101 70-71 -6.101396917550517E-4 1101 72-73 0.43923504588478224 1101 74-75 0.2844243342884347 1101 76 -1.3694477524820208 1102 1 -0.008935792570035517 1102 2 0.17981480407602035 1102 3 0.21205110699686003 1102 4 0.6832098754750646 1102 5 0.40275426238513035 1102 6 -0.25221081901997167 1102 7 -0.06467484487379949 1102 8 -1.9540624297339697 1102 9 -0.2855155196561654 1102 10-11 -1.1242425070005275 1102 12-13 -1.532534666305569 1102 14-15 0.0926314324317552 1102 16-17 -0.40105832241971484 1102 18-19 -0.6465277713329272 1102 20-21 -0.9973201415707997 1102 22-23 -0.14828907523970614 1102 24-25 -0.7928195762421844 1102 26-27 0.39216596693198724 1102 28-29 -0.10217082253347698 1102 30-31 -0.3143994955320899 1102 32-33 0.08427989675529801 1102 34-35 -0.28060949041829986 1102 36-37 -0.4729344480239632 1102 38-39 -0.8045058580909519 1102 40-41 -0.05663075849731314 1102 42-43 0.0909190977994534 1102 44-45 -0.24343019712073755 1102 46-47 -0.3372833397415924 1102 48-49 -0.5941432412102579 1102 50-51 -1.2418174880896942 1102 52-53 -0.6177827712382751 1102 54-55 -0.09579458801051288 1102 56-57 -0.1627816068552299 1102 58-59 -0.6371489894813962 1102 60-61 -0.07341218256601323 1102 62-63 -0.5972766762775947 1102 64-65 -0.798736726590338 1102 66-67 -0.23870231333910752 1102 68-69 -1.075593164648133 1102 70-71 0.19784480227616186 1102 72-73 -0.5312499101291159 1102 74-75 -1.0993082006417012 1102 76 -0.9527256411491294 1103 1 -0.9774906219091797 1103 2 -0.9938961617918061 1103 3 -1.3063221682391628 1103 4 -0.5241249102910928 1103 5 -0.8316683955669397 1103 6 -1.4725448785697175 1103 7 -1.1237162392093119 1103 8 -0.02523163815807905 1103 9 -1.1398365073104841 1103 10-11 -0.42064773575869907 1103 12-13 -0.7332971935386823 1103 14-15 -0.7365987781710004 1103 16-17 -0.9102812708583485 1103 18-19 -0.8102532615290023 1103 20-21 -0.39347119458459545 1103 22-23 -0.6117967005120413 1103 24-25 -0.7456881310715211 1103 26-27 -1.2409858122982236 1103 28-29 -1.0077626889096614 1103 30-31 -1.4745302144863395 1103 32-33 -1.200832666788635 1103 34-35 -0.20275909099927247 1103 36-37 -0.9121138234778527 1103 38-39 -0.5571202371759192 1103 40-41 -0.4160352610390703 1103 42-43 -0.6301048673421583 1103 44-45 -0.07255147526162631 1103 46-47 -0.5241025118548812 1103 48-49 -1.3262783174629789 1103 50-51 -0.17780151132861732 1103 52-53 -0.19672249527603825 1103 54-55 -0.45962901066846484 1103 56-57 -1.177088070181302 1103 58-59 -0.447352330076896 1103 60-61 -0.9097593140111826 1103 62-63 -0.9132897481730211 1103 64-65 -0.4698333133732042 1103 66-67 -0.5814038383935731 1103 68-69 -1.2621218501964258 1103 70-71 -1.4746679065110584 1103 72-73 -0.7611700263237431 1103 74-75 -0.8444062398573919 1103 76 0.364049957979411 1104 1 -0.16112821487740092 1104 2 0.6033993081202951 1104 3 -0.541886738895009 1104 4 -0.11202213814775419 1104 5 -0.1476927363377314 1104 6 -0.3797118833026438 1104 7 0.5643162151517416 1104 8 -0.322729947826776 1104 9 1.1118876306205507 1104 10-11 0.12671534740560375 1104 12-13 0.9591639288413027 1104 14-15 0.40180554907713883 1104 16-17 -0.05825287329242457 1104 18-19 0.7816331482073053 1104 20-21 0.08303320027679817 1104 22-23 0.012949749792223741 1104 24-25 0.19004547271481798 1104 26-27 0.2214550261061028 1104 28-29 0.5706686836393615 1104 30-31 0.8015008876096736 1104 32-33 0.014143668573098012 1104 34-35 0.014516094937171431 1104 36-37 1.3274061224315474 1104 38-39 0.05085812658337474 1104 40-41 -0.5373672421073614 1104 42-43 0.18510811440226504 1104 44-45 1.2684897688222136 1104 46-47 0.15473454020732547 1104 48-49 0.08433696313201722 1104 50-51 -0.5201274072042068 1104 52-53 -0.02944730933959505 1104 54-55 -0.6749772189172845 1104 56-57 -0.3274261364422877 1104 58-59 0.2343920918297968 1104 60-61 -0.5706450476149705 1104 62-63 -0.19401997129675408 1104 64-65 0.17752976979110002 1104 66-67 0.7418213137362457 1104 68-69 0.19845962377247162 1104 70-71 -0.237446811176369 1104 72-73 -0.12394893098905868 1104 74-75 0.5874734085536986 1104 76 0.39082480584959356 1105 1 -0.22888381076366926 1105 2 -0.2944379574574434 1105 3 -0.4481178338738374 1105 4 -1.9266016904768506 1105 5 -1.368200903306878 1105 6 -0.9079937949783847 1105 7 -0.6745676323981655 1105 8 -1.1835133719042084 1105 9 -0.2161522967841698 1105 10-11 -1.9623273023221657 1105 12-13 -1.0849225805355864 1105 14-15 -1.094300016561096 1105 16-17 -0.27138808200385967 1105 18-19 -0.8822733853680127 1105 20-21 -0.3332931760087483 1105 22-23 -1.1853261122767442 1105 24-25 -0.5314079453130098 1105 26-27 -0.7761251311836723 1105 28-29 -1.3098137725009913 1105 30-31 -1.391519378573026 1105 32-33 -1.1289673417112382 1105 34-35 -0.2578674501323981 1105 36-37 -0.20352249220849927 1105 38-39 -1.2811140452254541 1105 40-41 -0.35187117435176063 1105 42-43 -0.40518226672296365 1105 44-45 -0.5629152523514094 1105 46-47 0.1928092528510028 1105 48-49 -0.3099083484227343 1105 50-51 -0.34858324507474947 1105 52-53 -0.5320940123039009 1105 54-55 -0.37003922738673367 1105 56-57 -0.5490307946395063 1105 58-59 -0.4822594508199316 1105 60-61 -0.8293413573548385 1105 62-63 -0.8290018224764246 1105 64-65 0.06875027795807043 1105 66-67 0.01178501609559035 1105 68-69 0.052777530608526746 1105 70-71 -0.5864713120838125 1105 72-73 -0.5190260634754473 1105 74-75 -1.120141533975037 1105 76 -0.708086264930806 1106 1 0.6388492473718657 1106 2 0.5668881519336821 1106 3 0.31590005398306076 1106 4 -0.3084386357812896 1106 5 0.47029238874678825 1106 6 0.02157276848910783 1106 7 1.2207282052351331 1106 8 0.35385332916218104 1106 9 0.6287909436699088 1106 10-11 0.8365418067249628 1106 12-13 1.6052648979645845 1106 14-15 0.6745123329401075 1106 16-17 1.2567121931939376 1106 18-19 0.65902778422263 1106 20-21 0.8777706354807613 1106 22-23 1.087549704716718 1106 24-25 0.9824164440918786 1106 26-27 -0.22693352471652517 1106 28-29 0.8745902522668096 1106 30-31 1.7215482168862088 1106 32-33 0.6122392286362022 1106 34-35 0.7920121508307929 1106 36-37 0.538866568679012 1106 38-39 0.3425529654384576 1106 40-41 0.38853990235962144 1106 42-43 0.6486533025924821 1106 44-45 1.0437883940194226 1106 46-47 0.7340674227856425 1106 48-49 0.35574782632787105 1106 50-51 0.7333095997824941 1106 52-53 -0.04770288743289797 1106 54-55 1.293638963187746 1106 56-57 0.11245441347882945 1106 58-59 1.3163731601191841 1106 60-61 0.6954694441587499 1106 62-63 1.2507625394086794 1106 64-65 0.7370294317248387 1106 66-67 0.5581386452665598 1106 68-69 0.04604808444409514 1106 70-71 0.46291379283534795 1106 72-73 0.1515750717154738 1106 74-75 0.42193362942365553 1106 76 0.8032656442539192 1107 1 -2.110887206349407 1107 2 -1.7567045109379187 1107 3 -1.3275745401556698 1107 4 -2.8186220639912847 1107 5 -0.7303401223221577 1107 6 0.2318194478059965 1107 7 -1.8555947970840734 1107 8 -0.9922145603592192 1107 9 -0.855965539568551 1107 10-11 -0.1934181342406731 1107 12-13 -0.650754499041529 1107 14-15 -1.3369782848123677 1107 16-17 -0.13011049286973275 1107 18-19 -1.2696460509028142 1107 20-21 -0.7050461471462661 1107 22-23 -0.028874499373518603 1107 24-25 -0.44853443088176803 1107 26-27 -0.7433577288067603 1107 28-29 -0.5476108862531142 1107 30-31 -0.6018546926647055 1107 32-33 -0.6179104656501124 1107 34-35 -0.42628850276397756 1107 36-37 -0.15670585383838187 1107 38-39 -0.20522270396908837 1107 40-41 0.23424936894195625 1107 42-43 -0.3296304840404538 1107 44-45 -0.21145090600166583 1107 46-47 -0.4850133277941602 1107 48-49 -0.5258039341612744 1107 50-51 -0.48671991740071974 1107 52-53 -1.2396067457504216 1107 54-55 -0.25953413400812053 1107 56-57 -1.0192133073729543 1107 58-59 -0.11405062229700746 1107 60-61 -0.4558693709371795 1107 62-63 -0.21215122824892063 1107 64-65 -0.7780875828229163 1107 66-67 -1.0057871401013507 1107 68-69 -0.7252148293235621 1107 70-71 -0.28216316267803876 1107 72-73 -1.0041491534584672 1107 74-75 -0.3729641146201992 1107 76 -1.8260828693450684 1108 1 -0.3704878207887319 1108 2 0.9442390923098323 1108 3 0.6234897565307449 1108 4 -0.3544570431442331 1108 5 -0.1739653143344455 1108 6 -1.0040374756085306 1108 7 -1.3745765833469683 1108 8 -0.6806938230320299 1108 9 -0.1643263032288509 1108 10-11 -0.41222436641095683 1108 12-13 -0.7413404108255968 1108 14-15 -1.3775751687272226 1108 16-17 -1.5094609427270953 1108 18-19 -1.1210175672512932 1108 20-21 0.1329393696410932 1108 22-23 -1.4251220306440935 1108 24-25 -0.7360042575221009 1108 26-27 -0.6613739675603263 1108 28-29 -1.5585830170409132 1108 30-31 -0.9017010828336787 1108 32-33 -0.6801443914785104 1108 34-35 -1.2793497272537735 1108 36-37 -0.648608421316986 1108 38-39 -0.07434712793222431 1108 40-41 -0.9023097708429901 1108 42-43 -0.5560952635006231 1108 44-45 0.1641031866031213 1108 46-47 -0.6001329240197428 1108 48-49 -1.2026088496758653 1108 50-51 -0.8291820635495029 1108 52-53 -0.9576668730271365 1108 54-55 -0.912310083097438 1108 56-57 0.0629679522276625 1108 58-59 -0.5975924261153125 1108 60-61 -0.6455336237350728 1108 62-63 -1.3767551343274818 1108 64-65 0.10797781107658011 1108 66-67 -0.20223216972610558 1108 68-69 0.26466886609009066 1108 70-71 -1.1087815519692406 1108 72-73 -1.1755557806254622 1108 74-75 -0.8561109217301421 1108 76 0.11378985393779217 1109 1 0.5750904105037868 1109 2 0.3115860310853442 1109 3 -1.2404558217005466 1109 4 0.1081279908693702 1109 5 -1.1195435456269642 1109 6 -1.5890714892139748 1109 7 -0.03444052892519878 1109 8 0.12882998649178035 1109 9 -2.1031018134329322 1109 10-11 -0.48025157729531287 1109 12-13 0.17362557556895908 1109 14-15 -0.7653302707680396 1109 16-17 -0.659120806672675 1109 18-19 -0.16863661487034065 1109 20-21 -0.2820266167534662 1109 22-23 -1.7448499218005544 1109 24-25 -0.3890654820118975 1109 26-27 0.1243403181539584 1109 28-29 -0.8000796156803673 1109 30-31 -1.1023813549425192 1109 32-33 -0.6631375887574222 1109 34-35 -0.8847919041245227 1109 36-37 -0.5499689655346742 1109 38-39 -0.31924508711589894 1109 40-41 -0.5859832402307497 1109 42-43 -0.8452109097591247 1109 44-45 -0.6896383099955194 1109 46-47 0.2978262596537249 1109 48-49 -0.42029592450579756 1109 50-51 -0.3427875057263776 1109 52-53 -0.9032451043196517 1109 54-55 0.06388039309304006 1109 56-57 -0.7873721838267578 1109 58-59 -0.5193611335983057 1109 60-61 0.6639901857887338 1109 62-63 -0.7611088758240783 1109 64-65 -0.8239949780390639 1109 66-67 -0.34849067312746485 1109 68-69 -0.24213385499834672 1109 70-71 -1.5577611438059762 1109 72-73 -0.6891612228023369 1109 74-75 -1.0567911938389827 1109 76 0.41991230291738546 1110 1 1.0882309185055483 1110 2 0.330183398089698 1110 3 2.3171226053537275 1110 4 1.5084607858578707 1110 5 1.8421360902561688 1110 6 1.4667695522385777 1110 7 3.1546163888059233 1110 8 1.4868293013256277 1110 9 1.2112426293801626 1110 10-11 1.5164449430394384 1110 12-13 2.27994868035044 1110 14-15 2.46564413338211 1110 16-17 2.427234598798865 1110 18-19 1.8432803821442931 1110 20-21 1.2184627876413892 1110 22-23 1.6941702906009546 1110 24-25 2.1520689573753664 1110 26-27 2.1242913777144743 1110 28-29 1.7493257579798858 1110 30-31 1.7243694892800612 1110 32-33 1.8188033890725563 1110 34-35 1.8557258857252314 1110 36-37 2.396392312789608 1110 38-39 1.9564853489434206 1110 40-41 2.2259875912673195 1110 42-43 2.4683504860222065 1110 44-45 1.5037498366300355 1110 46-47 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30-31 -0.2710763558786766 2116 32-33 -0.20601345470013044 2116 34-35 -0.6262885027639769 2116 36-37 -1.1363448002722407 2116 38-39 -0.6903366430043079 2116 40-41 -1.9038388228307603 2116 42-43 -0.750908968799255 2116 44-45 -0.1959729539448425 2116 46-47 0.161272555603297 2116 48-49 0.13640381961203474 2116 50-51 -1.410721101182908 2116 52-53 -1.2642886042884491 2116 54-55 -0.703422858482817 2116 56-57 -0.6032618424424996 2116 58-59 -0.6676167661265424 2116 60-61 0.24652778798557762 2116 62-63 -0.49661408708289656 2116 64-65 -1.5836217645334791 2116 66-67 0.6360003709966087 2116 68-69 -0.2841671820906484 2116 70-71 -0.6222933787182896 2116 72-73 -0.5271441223841862 2116 74-75 0.5925878238231306 2116 76 0.308572354093819 2117 1 0.06825466649744527 2117 2 0.4302043522001959 2117 3 0.21360929949356233 2117 4 0.6911806294005132 2117 5 0.26107860614636635 2117 6 1.0147195417615222 2117 7 0.956180962389773 2117 8 1.3636889786323252 2117 9 -0.32818602187359147 2117 10-11 0.5898891003349647 2117 12-13 -0.03963642826169789 2117 14-15 0.5679700396473883 2117 16-17 1.304653052385511 2117 18-19 0.7669026493790483 2117 20-21 -0.4482970084052731 2117 22-23 1.0501107809271382 2117 24-25 0.3555682938285365 2117 26-27 0.9298023087754501 2117 28-29 0.7340991043513334 2117 30-31 -0.7523714480671515 2117 32-33 0.3824340379515121 2117 34-35 0.4610901380127217 2117 36-37 0.7557902313479623 2117 38-39 0.9457638290719359 2117 40-41 0.7514119120049045 2117 42-43 1.6860287705790284 2117 44-45 0.4277911860747565 2117 46-47 4.548839189659759E-4 2117 48-49 0.7345155146329638 2117 50-51 0.5062647365297508 2117 52-53 1.5797480929592567 2117 54-55 0.8037918916730575 2117 56-57 0.06597303775701846 2117 58-59 1.169695071407972 2117 60-61 0.330788944127022 2117 62-63 0.49145040031356046 2117 64-65 1.2863345906816228 2117 66-67 0.3514630947874693 2117 68-69 0.6056965392952947 2117 70-71 0.6539157599652441 2117 72-73 1.3547922809292565 2117 74-75 0.20570798058806972 2117 76 0.11128011217701328 2118 1 2.4805106431129573 2118 2 1.3701772094921907 2118 3 1.0702749222099222 2118 4 1.4480446026753846 2118 5 0.9105200927316055 2118 6 1.6812818133973941 2118 7 2.4024697190449587 2118 8 1.1626241493182015 2118 9 2.328980696990591 2118 10-11 1.813033478662554 2118 12-13 1.2449444256896562 2118 14-15 2.345817414112364 2118 16-17 2.060212087775426 2118 18-19 2.6841173899574002 2118 20-21 2.9702226700580354 2118 22-23 2.44101797374476 2118 24-25 1.7772720207311359 2118 26-27 1.5136315185050684 2118 28-29 1.3609912642845217 2118 30-31 1.3110485331417436 2118 32-33 1.5283260934896674 2118 34-35 3.029625475730647 2118 36-37 1.6561973719680694 2118 38-39 2.166820306783599 2118 40-41 2.162206358189266 2118 42-43 1.8316598385401939 2118 44-45 1.4240329649660808 2118 46-47 2.193481295861755 2118 48-49 3.1806476787419555 2118 50-51 2.9444628782982036 2118 52-53 1.6657695983356007 2118 54-55 1.7049819950241343 2118 56-57 2.4323995958528535 2118 58-59 1.0956267970578288 2118 60-61 0.9452058978800224 2118 62-63 1.2781713523962424 2118 64-65 2.175675858037298 2118 66-67 1.2114171609739195 2118 68-69 1.4479034502463293 2118 70-71 1.6361164072144305 2118 72-73 1.9216572981544324 2118 74-75 2.129186423014211 2118 76 3.0663902489345034 2119 1 0.5580836077826987 2119 2 -0.24963845871057444 2119 3 0.732333293945711 2119 4 -0.5143209887224671 2119 5 1.1117489713798392 2119 6 1.601404701262215 2119 7 -0.8252568554558088 2119 8 1.1526395103013058 2119 9 1.726830159356183 2119 10-11 1.1694082866502669 2119 12-13 1.4839997252288235 2119 14-15 0.9327989809326418 2119 16-17 1.0041444994497724 2119 18-19 0.7701388953337442 2119 20-21 0.5547080771240864 2119 22-23 0.23729293534230322 2119 24-25 0.10753315744388559 2119 26-27 -0.21409505599570267 2119 28-29 0.38614487411554776 2119 30-31 0.16377510764251113 2119 32-33 0.31730458811332696 2119 34-35 0.7784734019979282 2119 36-37 0.6260514426285937 2119 38-39 -0.2945852231703192 2119 40-41 0.9667378482046267 2119 42-43 0.486421743302099 2119 44-45 0.24488549952829075 2119 46-47 0.689833062374813 2119 48-49 -0.5240374211044383 2119 50-51 0.15721249427362238 2119 52-53 -0.6583471451359806 2119 54-55 -0.6487046409205632 2119 56-57 -0.4013177620580528 2119 58-59 0.6668633561976165 2119 60-61 -0.24757444006160512 2119 62-63 -0.14291159691251565 2119 64-65 0.6964131852262376 2119 66-67 0.4907950411582469 2119 68-69 -0.07206582778746196 2119 70-71 0.8027830738811019 2119 72-73 0.1841380969255546 2119 74-75 0.3709298945963937 2119 76 0.7073272689037786 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 36.0 13 438.0 14 1993.0 15 3575.0 16 4418.0 17 5087.0 18 5122.0 19 4478.0 20 3276.0 21 2187.0 22 1409.0 23 969.0 24 673.0 25 511.0 26 387.0 27 304.0 28 280.0 29 257.0 30 316.0 31 344.0 32 432.0 33 586.0 34 854.0 35 1252.0 36 2686.0 37 9647.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.427415128399232 21.856014285991577 11.904345969448164 45.81222461616103 2 9.953609348007532 21.64055979347425 39.30976921135891 29.0960616471593 3 15.68353423008987 23.740755837652127 33.08294027446185 27.492769657796153 4 9.88567324676333 25.00436732079427 33.13728915545721 31.972670276985188 5 9.031619402550515 30.22574195927716 37.09699334226208 23.64564529591025 6 13.684271822046234 38.22085055998758 21.47169005609581 26.623187561870377 7 12.434247559152933 38.042275665288535 20.41382790815039 29.10964886740814 8 11.5879578407966 27.83827325840952 20.763213571691995 39.81055532910188 9 14.967293619829578 23.87468700867641 21.182476367941923 39.97554300355209 10-11 13.187367767231507 26.206836312816627 29.501737223160383 31.104058696791476 12-13 11.796618723189503 22.318950290184205 30.612977736369107 35.27145325025718 14-15 16.027096799239114 24.012500242628935 28.220656456841166 31.73974650129079 16-17 19.299675847745494 24.213396999165358 25.313961839321415 31.172965313767737 18-19 16.646285836293405 22.630485840175467 26.37667656592713 34.34655175760399 20-21 16.74916050389177 22.156874162930183 27.7878064403424 33.306158892835654 22-23 21.068926027290903 22.06467516838448 23.720375007278868 33.14602379704575 24-25 17.29847240823774 27.063801704225625 22.58001902210835 33.05770686542829 26-27 16.122207340981 27.493740173528213 26.067082047399985 30.316970438090802 28-29 17.92833711834469 24.36091539043848 29.600729827830506 28.110017663386323 30-31 17.89454273707503 23.599289575589353 30.48807709851801 28.018090588817607 32-33 15.23030338321784 25.935091907839826 29.818125351811954 29.016479357130383 34-35 14.798423882451134 26.963838583823446 28.232302645625886 30.005434888099536 36-37 16.34639647508686 22.346124730681886 30.205361128903903 31.102117665327356 38-39 15.596187814204468 20.574933519672353 35.12878743764436 28.700091228478815 40-41 14.322871173741728 21.156272443176306 33.6739843552864 30.84687202779557 42-43 11.441409965255536 21.98800442555174 33.28189599953415 33.288689609658576 44-45 11.62580795434694 20.136260408781226 37.88602263242687 30.35190900444496 46-47 12.523535006502456 24.123139036083774 34.47077777130767 28.8825481861061 48-49 13.105844445738466 25.035423824220192 31.213726974514255 30.64500475552709 50-51 13.89209701369411 26.199326455545098 27.51438803536594 32.39418849539486 52-53 11.330771171800695 26.59213105844446 29.343543158834606 32.733554610920244 54-55 12.13047613501815 23.24579281430152 29.737572546050973 34.886158504629364 56-57 14.212232380286885 25.55367922514024 27.411246336303112 32.822842058269764 58-59 13.964750868611581 24.256099691375997 27.050214483976788 34.72893495603564 60-61 11.675304256682 22.211223043925543 29.315398202604865 36.79807449678759 62-63 13.99483685630544 23.34672645043576 26.759059764358778 35.89937692890002 64-65 14.107416681224402 23.16524000854054 25.69828606921718 37.029057241017874 66-67 12.696286806809137 28.59721656088045 21.51730429550263 37.18919233680778 68-69 14.239545017809135 28.585847802245794 21.5000436736318 35.67456350631327 70-71 15.374910227294786 27.21617267415905 21.297967740057068 36.1109493584891 72-73 15.313767736175004 28.857314777072535 21.56680059783769 34.26211688891477 74-75 14.683903026068052 26.12337195985947 24.48028882548186 34.712436188590615 76 12.733166404627418 24.315301151031658 21.102894077913003 41.84863836642792 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 2.0 12 3.0 13 3.0 14 5.5 15 10.5 16 18.0 17 23.0 18 30.5 19 44.0 20 62.5 21 75.0 22 101.0 23 155.0 24 213.0 25 243.0 26 271.5 27 344.0 28 455.5 29 523.0 30 579.0 31 730.0 32 897.5 33 970.0 34 1046.0 35 1274.0 36 1587.0 37 1748.0 38 1822.0 39 2003.5 40 2213.5 41 2435.5 42 2555.0 43 2626.0 44 2769.5 45 2866.0 46 2890.0 47 2798.5 48 2679.0 49 2555.5 50 2460.0 51 2366.5 52 2167.5 53 1936.5 54 1811.0 55 1735.5 56 1514.0 57 1249.0 58 1130.0 59 1096.0 60 1024.0 61 945.0 62 904.0 63 800.0 64 670.0 65 613.5 66 539.5 67 496.0 68 466.5 69 387.0 70 327.0 71 317.0 72 295.5 73 235.5 74 154.0 75 111.0 76 104.0 77 80.5 78 50.5 79 37.0 80 35.5 81 21.5 82 9.5 83 10.0 84 11.0 85 9.0 86 7.5 87 9.0 88 8.0 89 7.0 90 6.0 91 6.0 92 7.0 93 7.5 94 6.5 95 6.0 96 7.0 97 6.5 98 4.5 99 6.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 9.705157320600167E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 9.705157320600167E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 9.705157320600167E-4 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 51519.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.16174615190512 #Duplication Level Percentage of deduplicated Percentage of total 1 94.25993832819401 87.81420446825443 2 4.542045170430869 8.462897183563346 3 0.943828652387699 2.6378617597391254 4 0.19168264022001832 0.7142995787961723 5 0.041670139178264856 0.19410314641200332 6 0.006250520876739729 0.0349385663541606 7 0.006250520876739729 0.0407616607465207 8 0.0 0.0 9 0.0 0.0 >10 0.008334027835652971 0.10093363613424174 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0019410314641200334 0.0 2 0.0 0.0 0.0 0.0038820629282400668 0.0 3 0.0 0.0 0.0 0.0077641258564801335 0.0 4 0.0 0.0 0.0 0.015528251712960267 0.0 5 0.0 0.0 0.0 0.021351346105320367 0.0 6 0.0 0.0 0.0 0.021351346105320367 0.0 7 0.0 0.0 0.0 0.021351346105320367 0.0 8 0.0 0.0 0.0 0.021351346105320367 0.0 9 0.0 0.0 0.0 0.021351346105320367 0.0 10 0.0 0.0 0.0 0.0291154719618005 0.0 11 0.0 0.0 0.0 0.03299753489004057 0.0 12 0.0 0.0 0.0 0.03882062928240067 0.0 13 0.0 0.0 0.0 0.04464372367476077 0.0 14 0.0 0.0 0.0 0.0465847551388808 0.0 15 0.0 0.0 0.0 0.05046681806712087 0.0 16 0.0 0.0 0.0 0.052407849531240905 0.0 17 0.0 0.0 0.0 0.052407849531240905 0.0 18 0.0 0.0 0.0 0.0698771327083212 0.0 19 0.0 0.0 0.0 0.10481569906248181 0.0 20 0.0 0.0 0.0 0.10869776199072187 0.0 21 0.0 0.0 0.0 0.1106387934548419 0.0 22 0.0 0.0 0.0 0.1222849822395621 0.0 23 0.0 0.0 0.0 0.13004910809604223 0.0 24 0.0 0.0 0.0 0.13393117102428231 0.0 25 0.0 0.0 0.0 0.1514004542013626 0.0 26 0.0 0.0 0.0 0.15916458005784273 0.0 27 0.0 0.0 0.0 0.17663386323492303 0.0 28 0.0 0.0 0.0 0.2154544925173237 0.0 29 0.0 0.0 0.0 0.3047419398668452 0.0 30 0.0 0.0 0.0 0.36879597818280635 0.0 31 0.0 0.0 0.0 0.43673207942700754 0.0 32 0.0 0.0 0.0 0.5415477784894893 0.0 33 0.0 0.0 0.0 0.6774199809778917 0.0 34 0.0 0.0 0.0 0.9122847881364157 0.0 35 0.0 0.0 0.0 1.1257982491896195 0.0 36 0.0 0.0 0.0 1.4227760631999844 0.0 37 0.0 0.0 0.0 1.7469283177080301 0.0 38 0.0 0.0 0.0 2.0147906597565948 0.0 39 0.0 0.0 0.0 2.2399503095945184 0.0 40 0.0 0.0 0.0 2.4728740852889226 0.0 41 0.0 0.0 0.0 2.6669772317009257 0.0 42 0.0 0.0 0.0 2.8746675983617695 0.0 43 0.0 0.0 0.0 3.1735864438362547 0.0 44 0.0 0.0 0.0 3.429802597100099 0.0 45 0.0 0.0 0.0 3.7015470020769037 0.0 46 0.0 0.0 0.0 4.012112036336109 0.0 47 0.0 0.0 0.0 4.318795007667075 0.0 48 0.0 0.0 0.0 4.534249500184398 0.0 49 0.0 0.0 0.0 4.728352646596401 0.0 50 0.0 0.0 0.0 4.939925076185485 0.0 51 0.0 0.0 0.0 5.153438537238689 0.0 52 0.0 0.0 0.0 5.335895494865972 0.0 53 0.0 0.0 0.0 5.541644830062696 0.0 54 0.0 0.0 0.0 5.786214794541819 0.0 55 0.0 0.0 0.0 5.986141035346183 0.0 56 0.0 0.0 0.0 6.209359653719987 0.0 57 0.0 0.0 0.0 6.39958073720375 0.0 58 0.0 0.0 0.0 6.591742852151634 0.0 59 0.0 0.0 0.0 6.799433218812477 0.0 60 0.0 0.0 0.0 6.96053883033444 0.0 61 0.0 0.0 0.0 7.158524039674683 0.0 62 0.0 0.0 0.0 7.344863060230207 0.0 63 0.0 0.0 0.0 7.57002271006813 0.0 64 0.0 0.0 0.0 7.762184825016013 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGTTAA 20 7.8570425E-5 70.0 1 ACTCTGT 25 2.3695813E-4 56.000004 8 CGAACAT 20 0.0069145802 52.5 25 CCGATTA 20 0.0069145802 52.5 17 CTGAGCA 20 0.0069145802 52.5 2 CGATTAC 20 0.0069145802 52.5 18 TTTCAGC 20 0.0069145802 52.5 30 AAGCAGC 20 0.0069145802 52.5 57 CGCTACA 20 0.0069145802 52.5 17 TCCCGCA 20 0.0069145802 52.5 13 GAATTTA 20 0.0069145802 52.5 26 TTAGATC 35 0.0012446351 40.0 38 GAACATA 35 0.0012446351 40.0 26 CGACGAA 65 4.7507092E-7 37.69231 22 TTGTAGA 40 0.0023981119 35.0 36 GCCGACT 40 0.0023981119 35.0 22 CCGACTA 40 0.0023981119 35.0 23 TAGATCT 135 7.2759576E-12 31.11111 39 GTTCTTC 45 0.004270723 31.111109 52 CTCTAAT 45 0.004270723 31.111109 1 >>END_MODULE