FastQCFastQC Report
Wed 25 May 2016
SRR1780348_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780348_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51519
Sequences flagged as poor quality0
Sequence length76
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT1867736.25264465536986No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG26535.149556474310449No Hit
CACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCTG24934.8389914400512435Illumina PCR Primer Index 9 (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT14992.90960616471593Illumina PCR Primer Index 9 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC7901.5334148566548265No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT7481.451891535161785No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC6231.209262602146781No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC5761.1180341233331392No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT5441.055921116481298No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC5020.9743977949882567No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG3520.6832430753702518No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT3450.6696558551214116No Hit
TATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCT3260.6327762573031309Illumina PCR Primer Index 9 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC2310.4483782682117277No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG2030.39402938721636677No Hit
CTTATAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG1980.3843242298957666No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGT1680.3260932859721656No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCG1590.3086240027950853No Hit
CTTATTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG1230.2387468700867641No Hit
TTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC980.19022108348376326No Hit
CTTATACCACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG900.174692831770803No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCG750.1455773598090025No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG750.1455773598090025No Hit
CTTATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC630.1222849822395621No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCG590.11452085638308197No Hit
ACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCT580.11257982491896193Illumina PCR Primer Index 9 (95% over 24bp)
CACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGTCTTCTG520.10093363613424174TruSeq Adapter, Index 9 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCCT150.00221282470.069
TTGGCAC150.00221282470.046
CTTTACA1050.063.3333321
TTTACAC1050.063.3333322
TCTTTAT750.060.6666681
AACTCGT553.45608E-1157.2727337
GAACTCG553.45608E-1157.2727336
CTCGAAA252.3695813E-456.00000457
CGCCTTC252.3695813E-456.00000448
CCGCCTT252.3695813E-456.00000447
ATTACAC252.3695813E-456.0000044
GCTCGAA252.3695813E-456.00000456
CAGACCT1000.056.00000434
CGAAAAA252.3695813E-456.00000459
GCCGCCT252.3695813E-456.00000446
CTATACA750.056.01
CCTCGTA950.055.2631638
AGACCTC950.055.2631635
ACAGACC1050.053.33333233
ACTCGTA608.185452E-1152.538
Produced by FastQC (version 0.11.5)