FastQCFastQC Report
Wed 25 May 2016
SRR1780347_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780347_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153770
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCTC300.00571284329.35092237
TTAATAC454.0752333E-529.3031983
ACGAGAC804.2400643E-927.50753224
TTTGACG407.006153E-427.49857511
TTGACGC450.001387812224.45910312
CTTTATA551.5923825E-423.9831432
TTACACA1202.3646862E-1123.8088473
ATCTCGG3050.022.3449542
TGACGCC803.5529702E-622.02036513
ATTTGAC500.002571063821.99886110
CGAAAAT1105.185939E-921.99170325
AACGGCT1004.5762135E-821.99170329
CTTAATA500.002580805421.9845492
CCAATGT703.209232E-521.98454931
TTATACC500.002580805421.9845492
CAATGTG703.209232E-521.98454932
TTTACAC1205.9117156E-1021.9845472
TCGGTGG1205.9117156E-1021.98454742
AGAACGG1158.6602086E-921.0355427
CTTTACA1158.691131E-921.0286961