FastQCFastQC Report
Wed 25 May 2016
SRR1780347_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780347_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153770
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT5669536.87000065032191No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG83465.42758665539442No Hit
CACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTTCTG42212.7450087793457763RNA PCR Primer, Index 36 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTT25031.62775573909085RNA PCR Primer, Index 15 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC22261.44761657020225No Hit
CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC13520.8792352214346102No Hit
CTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC12250.7966443389477792No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC11160.7257592508291605No Hit
TATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCT6630.43116342589581846No Hit
CTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG6450.4194576315276062No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGT5440.35377511868374845No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC4790.3115041945763153No Hit
CTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG4780.3108538726669702No Hit
CTTATAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG3840.24972361318852834No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACATCTCGTATGCCGTC3760.24452103791376734No Hit
CTTATTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG2630.1710346621577681No Hit
CCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG2070.13461663523444106No Hit
TTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC1940.1261624504129544No Hit
CTTATACCACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG1580.10275086167652989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACA1500.042.586531
TTTACAC1600.041.2612882
CCTTATA404.053618E-738.5481531
TCTTCTG4550.038.19582744
CGTCTTC4600.037.78065542
GTCTTCT4700.036.9768143
ATGCCGT70450.036.34730544
CTATACA1950.036.1477171
CTTATAA505.8240403E-835.2440261
CGCGTGC250.002347027235.198186
CTTATAC74800.034.6020431
TTATACA76050.033.9422232
TCTTTAT1450.033.421061
TATACAC78100.033.0794683
ATACACA81450.031.7459474
TATGCCG81050.031.6208243
TACACAT82950.031.24135
ACACATC83100.031.2378546
CTTTATA1550.031.2343482
TTACACA2050.031.1304653
Produced by FastQC (version 0.11.5)