FastQCFastQC Report
Wed 25 May 2016
SRR1780346_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780346_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17686
Sequences flagged as poor quality0
Sequence length76
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTAGA200.006839728452.50000436
GTATCAT200.006839728452.50000458
TAACCAA200.006839728452.50000466
TCGTCGA200.006839728452.50000426
CCCCCCG200.006839728452.50000459
CTCGTCG200.006839728452.50000425
TAGATCT500.00702264827.99999839
CTTATAC3158.5128704E-1015.5555551
TATACAC3252.2326276E-814.03
CTGCCGA2001.6047717E-413.99999918
GCTGCCG2051.9792817E-413.65853717
TGCCGAC1850.001445647613.24324319
TTATACA3253.5018638E-712.9230782
GCCGACG1950.002149003612.56410220
CACATCT2851.7777958E-512.2807027
CGCTGCC2100.003746512311.66666716
ATACACA3053.563856E-511.47540954
CCGACGA2150.004467036611.39534923
TACACAT3156.2623573E-410.05
ACACATC3050.0055097229.1803276
Produced by FastQC (version 0.11.5)