FastQCFastQC Report
Wed 25 May 2016
SRR1780346_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780346_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17686
Sequences flagged as poor quality0
Sequence length76
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT556931.488182743412867No Hit
CACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCTG219012.38267556259188RNA PCR Primer, Index 32 (96% over 26bp)
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG13697.740585774058577No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC9125.156621056202646No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT4652.6291982358927966RNA PCR Primer, Index 42 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC4362.46522673300916No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT2231.2608843152776206No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT2201.2439217460137961No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT1981.1195295714124165No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC1520.8594368427004411No Hit
CTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG1240.7011195295714123No Hit
CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG1240.7011195295714123No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC1020.5767273549700328RNA PCR Primer, Index 32 (95% over 21bp)
CTTATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC920.5201854574239512No Hit
TATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCT850.480606129141694RNA PCR Primer, Index 42 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC810.45798937012326135No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCG690.39013909306796335No Hit
CACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGTCTTCTG680.38448490331335516RNA PCR Primer, Index 42 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAGCTCGTATGCCGT630.3562139545403144No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGC540.3053262467488409No Hit
ACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCT530.2996720569942327RNA PCR Primer, Index 42 (95% over 24bp)
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCG480.2714011082211919No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCG440.24878434920275921No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCC380.21485921067511027No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCC380.21485921067511027No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCC330.18658826190206945No Hit
CTTATTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG310.17527988239285308No Hit
CACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGTCTTCTG310.17527988239285308RNA PCR Primer, Index 42 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC310.17527988239285308RNA PCR Primer, Index 32 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG300.16962569263824495No Hit
CTCTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG300.16962569263824495No Hit
TCTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT260.1470089336198123No Hit
CTTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC240.13570055411059595No Hit
ACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCTGC220.12439217460137961RNA PCR Primer, Index 32 (96% over 27bp)
CTTATAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG200.11308379509216329No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACCCGATCTCGTATGCCGT190.10742960533755513No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGCTCTCGTATGCCGT180.10177541558294696No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACATCGTATGCCGT180.10177541558294696No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAAATCGTATGCCGT180.10177541558294696No Hit
TCCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC180.10177541558294696No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCAAA150.002188686770.058
TGCAAAA150.002188686770.059
ACACCAA150.002188686770.070
TGCTTGC150.002188686770.055
GCTTGCA150.002188686770.056
CTTGCAA150.002188686770.057
TCTTTAT600.070.01
TTTATAC700.065.03
CTTTATA650.064.615392
ACTCGAA352.8103932E-760.032
ACTCGTA308.044723E-658.33333238
AACTCGT308.044723E-658.33333237
CGAACTC308.044723E-658.33333235
GAACTCG308.044723E-658.33333236
TCTTAAT308.044723E-658.3333321
TGCGCAG200.006839728452.50000431
AAAACCT200.006839728452.50000467
CTCGAAC352.0043413E-550.033
ACTCGAC352.0043413E-550.032
CCTCGTA352.0043413E-550.038
Produced by FastQC (version 0.11.5)