##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780346_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17686 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.53822232274115 34.0 34.0 34.0 34.0 34.0 2 33.501526631233745 34.0 34.0 34.0 34.0 34.0 3 33.507293904783445 34.0 34.0 34.0 34.0 34.0 4 33.63869727468054 34.0 34.0 34.0 34.0 34.0 5 33.565418975460815 34.0 34.0 34.0 34.0 34.0 6 37.31420332466357 38.0 38.0 38.0 36.0 38.0 7 37.25930114214633 38.0 38.0 38.0 36.0 38.0 8 37.17403596064684 38.0 38.0 38.0 36.0 38.0 9 37.23187832183648 38.0 38.0 38.0 36.0 38.0 10-11 37.14997738324098 38.0 38.0 38.0 36.0 38.0 12-13 37.07734931584304 38.0 38.0 38.0 36.0 38.0 14-15 36.962738889517134 38.0 37.0 38.0 35.0 38.0 16-17 36.927513287345924 38.0 37.0 38.0 34.5 38.0 18-19 36.67378152210788 38.0 37.0 38.0 34.0 38.0 20-21 36.79475291190772 38.0 37.0 38.0 34.0 38.0 22-23 36.912444871649896 38.0 37.0 38.0 35.0 38.0 24-25 36.748671265407665 38.0 37.0 38.0 34.0 38.0 26-27 36.48696709261563 38.0 37.0 38.0 34.0 38.0 28-29 36.30269704851295 38.0 37.0 38.0 33.5 38.0 30-31 36.16645934637566 38.0 37.0 38.0 33.0 38.0 32-33 35.32316521542463 38.0 36.0 38.0 29.0 38.0 34-35 35.762015153228546 38.0 36.5 38.0 32.0 38.0 36-37 35.868087753024994 38.0 36.5 38.0 32.5 38.0 38-39 31.469834897659165 36.5 27.0 38.0 11.0 38.0 40-41 33.74754042745674 37.0 33.0 38.0 24.0 38.0 42-43 35.87439217460138 37.5 36.0 38.0 32.5 38.0 44-45 36.30346036412982 38.0 36.5 38.0 33.5 38.0 46-47 36.08845979871084 38.0 37.0 38.0 33.0 38.0 48-49 36.216753364242905 38.0 36.5 38.0 33.0 38.0 50-51 35.44902747936221 38.0 36.0 38.0 31.0 38.0 52-53 35.36427117494063 38.0 36.0 38.0 31.0 38.0 54-55 35.3571751667986 38.0 36.0 38.0 29.0 38.0 56-57 35.44993214972294 38.0 36.0 38.0 31.0 38.0 58-59 34.878519733122246 37.5 35.0 38.0 27.0 38.0 60-61 34.87046251272193 37.0 35.0 38.0 27.0 38.0 62-63 35.09866561121791 37.5 36.0 38.0 27.0 38.0 64-65 34.32409815673414 37.5 35.0 38.0 24.5 38.0 66-67 32.51150627615063 37.0 31.0 38.0 16.0 38.0 68-69 31.66431075426891 37.0 28.5 38.0 11.0 38.0 70-71 28.187097138979986 32.0 18.0 37.5 10.5 38.0 72-73 22.652239059142826 22.5 11.0 32.5 10.0 37.5 74-75 21.238550265746916 21.0 11.0 30.5 10.5 37.0 76 21.159335067284857 21.0 11.0 29.0 11.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21602634236380425 1101 2 0.3064562369826547 1101 3 -0.09851671641783355 1101 4 -0.020919836726911 1101 5 -0.19005605870171394 1101 6 -0.20134091197986237 1101 7 -0.1436054870781689 1101 8 0.1379883345651649 1101 9 0.34657977227294623 1101 10-11 0.261031081637519 1101 12-13 0.38426571566927237 1101 14-15 0.037313658107059666 1101 16-17 0.260616509440581 1101 18-19 -0.003995330194740632 1101 20-21 0.3488554206286949 1101 22-23 0.5706079672464242 1101 24-25 -0.06657141925894905 1101 26-27 -0.7687938179243545 1101 28-29 -0.41499007239485763 1101 30-31 0.3416717131624978 1101 32-33 0.48625672933199127 1101 34-35 0.8352740620337471 1101 36-37 1.0100202247731929 1101 38-39 1.7521862918241489 1101 40-41 1.2470303004695253 1101 42-43 0.6813957256635277 1101 44-45 -0.5772362112687617 1101 46-47 -0.6616817495398806 1101 48-49 0.5741688258397488 1101 50-51 0.6306973043259276 1101 52-53 0.8260158253454364 1101 54-55 1.0670996982119263 1101 56-57 0.9312072003066163 1101 58-59 1.2988271156535163 1101 60-61 0.6780924680168283 1101 62-63 0.31766636111704116 1101 64-65 0.5054462962939752 1101 66-67 1.3491893844591125 1101 68-69 -0.37552937153933286 1101 70-71 -0.7016663068800284 1101 72-73 0.8781769736136091 1101 74-75 0.9364466226675958 1101 76 -0.27436775874684827 1102 1 0.059870186207646725 1102 2 -0.5723511418247185 1102 3 -0.23086334876446557 1102 4 0.13738132157424587 1102 5 -0.11390848255413744 1102 6 0.2757075650686147 1102 7 0.376343032870345 1102 8 0.050901247478080336 1102 9 -0.16371623802306345 1102 10-11 -0.19392387331744487 1102 12-13 0.22896756037111743 1102 14-15 -0.21290084210744453 1102 16-17 -0.004720255896188519 1102 18-19 0.4983641721647558 1102 20-21 0.44462976640303964 1102 22-23 -0.021841625203165904 1102 24-25 0.157259554572029 1102 26-27 0.3920813429508101 1102 28-29 0.20180529440051487 1102 30-31 0.14937227086305427 1102 32-33 0.8416835847588473 1102 34-35 0.7945190212787026 1102 36-37 0.549381014133985 1102 38-39 0.46818800782586223 1102 40-41 0.24005904349827034 1102 42-43 0.4379379822057814 1102 44-45 0.39745276342021896 1102 46-47 0.19127835342022337 1102 48-49 0.483649735320661 1102 50-51 0.36900704263567263 1102 52-53 0.05510205443166427 1102 54-55 0.4658555969678204 1102 56-57 1.3476593667587906 1102 58-59 1.403824970651364 1102 60-61 -0.23932494940059001 1102 62-63 0.35338064683132586 1102 64-65 1.3654849063325827 1102 66-67 2.7106222458919724 1102 68-69 2.8108713148613553 1102 70-71 1.9410835858698654 1102 72-73 -0.21920612376948867 1102 74-75 0.026643962864934423 1102 76 -0.5459250303041188 1103 1 0.07060150576249669 1103 2 0.3410033891768691 1103 3 -0.41130849979785467 1103 4 -0.0923484081554804 1103 5 0.14841266211994508 1103 6 0.0292846715869004 1103 7 0.1911284064792511 1103 8 0.16576611234294347 1103 9 -0.015465045654224241 1103 10-11 0.3026977483041833 1103 12-13 0.52268775115013 1103 14-15 -0.1145304128546556 1103 16-17 0.25303014156008885 1103 18-19 0.5265835680312136 1103 20-21 0.672968399153433 1103 22-23 0.2287545592165472 1103 24-25 0.1658048645879262 1103 26-27 0.4304358739523977 1103 28-29 0.3119006839076661 1103 30-31 0.29802091951169984 1103 32-33 0.8859999599575801 1103 34-35 0.5143123440132058 1103 36-37 1.1859072462484548 1103 38-39 -0.554069543843454 1103 40-41 0.45548034715486807 1103 42-43 0.7574928311723923 1103 44-45 0.8507516505426338 1103 46-47 0.9072958414965342 1103 48-49 0.4739120564653376 1103 50-51 0.3809307310299488 1103 52-53 0.2454836124602835 1103 54-55 0.47022761604572594 1103 56-57 0.6592650901292103 1103 58-59 1.7551763220027183 1103 60-61 1.0057068471017914 1103 62-63 1.662204176243094 1103 64-65 1.8426311702435498 1103 66-67 1.4049783667186837 1103 68-69 1.9152035883112681 1103 70-71 1.7365923299080208 1103 72-73 1.6394981687583332 1103 74-75 0.0037902267759086783 1103 76 1.4616266390122554 1104 1 0.39279401913147893 1104 2 -0.060066129539706026 1104 3 0.4808483629472491 1104 4 0.21068189487482414 1104 5 0.36766399901834035 1104 6 0.5836518730129256 1104 7 0.5360481925755067 1104 8 0.4006145971914279 1104 9 0.38337630906948306 1104 10-11 0.6019401725466054 1104 12-13 0.36983570123925347 1104 14-15 0.649507020300419 1104 16-17 0.6422903911144573 1104 18-19 0.8149079887085762 1104 20-21 0.5461858679591387 1104 22-23 0.6986743453128028 1104 24-25 0.5376854349979041 1104 26-27 0.4270936279630959 1104 28-29 0.15102724362246533 1104 30-31 0.7374148589056375 1104 32-33 0.9000373931126546 1104 34-35 0.6823159090755908 1104 36-37 0.5399625047154757 1104 38-39 0.5613493509872036 1104 40-41 0.8971024505416736 1104 42-43 0.9526799969478006 1104 44-45 0.7903684063358583 1104 46-47 -0.2947986326567644 1104 48-49 1.2000707017416232 1104 50-51 0.7717506953793247 1104 52-53 1.06411106344067 1104 54-55 0.3102454413576723 1104 56-57 0.3994611685605918 1104 58-59 0.9976005644269605 1104 60-61 0.9609207508451121 1104 62-63 0.11095640440708365 1104 64-65 0.3820696729173534 1104 66-67 1.250343785613513 1104 68-69 0.9390449430349825 1104 70-71 0.7965299413162228 1104 72-73 1.0488118942485265 1104 74-75 1.399289335510307 1104 76 0.5806105962315087 1105 1 -0.031448405110943156 1105 2 0.21266114318756024 1105 3 0.16266654476542897 1105 4 0.18037886457179297 1105 5 0.3373609687153092 1105 6 -0.24968146032041005 1105 7 0.49059364712096 1105 8 0.08243277900960777 1105 9 0.3379217636149363 1105 10-11 0.5337583543647924 1105 12-13 0.6880175194210807 1105 14-15 0.39193126272466117 1105 16-17 0.5665328153568794 1105 18-19 0.9891504129509983 1105 20-21 0.5461858679591387 1105 22-23 0.6607955574340139 1105 24-25 0.507382404694873 1105 26-27 0.20739665826612708 1105 28-29 1.1055726981679186 1105 30-31 0.8434754649662466 1105 32-33 1.248522241597506 1105 34-35 0.12170984846952848 1105 36-37 0.638447353200327 1105 38-39 -0.46895367931582754 1105 40-41 0.9274054808447048 1105 42-43 0.7178315120993162 1105 44-45 0.5403684063358654 1105 46-47 0.6976256097674849 1105 48-49 0.9803737320446544 1105 50-51 0.3853870590156916 1105 52-53 0.7913837907134038 1105 54-55 0.27236665347888334 1105 56-57 0.6418854109848269 1105 58-59 0.5430551098815002 1105 60-61 1.5442540841784478 1105 62-63 0.7927745862252635 1105 64-65 0.7608575517052287 1105 66-67 1.068525603795333 1105 68-69 1.7648025187925604 1105 70-71 2.031378426164707 1105 72-73 0.3139634094000421 1105 74-75 -0.6310136947927205 1105 76 -0.5860560704351592 1106 1 -0.6423965967042946 1106 2 -0.6656379770470409 1106 3 -0.3686140026441507 1106 4 -0.41492905331676866 1106 5 0.009892738021271441 1106 6 -0.022897491600957665 1106 7 0.01845288465761996 1106 8 -0.19713711346350493 1106 9 0.19373799039890116 1106 10-11 0.16156871604611922 1106 12-13 0.09099894659605923 1106 14-15 -0.0858302231013397 1106 16-17 -0.06030002921789901 1106 18-19 -0.14134812077334402 1106 20-21 0.44550160598455335 1106 22-23 0.20527258578201213 1106 24-25 0.4743912023488335 1106 26-27 -0.09636678259409592 1106 28-29 0.41911131009362634 1106 30-31 0.4835047904794436 1106 32-33 1.2372807948721842 1106 34-35 0.9137430840511485 1106 36-37 0.9698256523205586 1106 38-39 0.6613004751318776 1106 40-41 1.2367896450675815 1106 42-43 0.3808324896164166 1106 44-45 0.9159793545274582 1106 46-47 0.09254252081342429 1106 48-49 0.8171283752509098 1106 50-51 0.548388036532792 1106 52-53 1.335616439784765 1106 54-55 1.0802845420419303 1106 56-57 0.33763321157134385 1106 58-59 0.8196924510349746 1106 60-61 0.8142931848627057 1106 62-63 1.0227354855410056 1106 64-65 0.7992250981372919 1106 66-67 0.5966292206086301 1106 68-69 1.846180817912792 1106 70-71 0.8876834115019498 1106 72-73 0.42246780822702235 1106 74-75 -0.12026100662067662 1106 76 -1.9003278201907783 1107 1 -0.2694437431062795 1107 2 -0.1929332624068394 1107 3 0.4167457988446799 1107 4 0.20765159184451676 1107 5 -0.18688145552711433 1107 6 0.44285933222037954 1107 7 0.4856985422258546 1107 8 0.16191995849678875 1107 9 0.26892409461726885 1107 10-11 0.21231313291956866 1107 12-13 0.25142078282434 1107 14-15 0.5491573699507697 1107 16-17 0.07282652165058323 1107 18-19 -0.32658385278327273 1107 20-21 0.3644842362575105 1107 22-23 0.606017002655463 1107 24-25 0.6014383487508255 1107 26-27 0.12534537621484532 1107 28-29 0.8553395979348153 1107 30-31 0.47109784258861964 1107 32-33 1.8919954350706973 1107 34-35 0.4394254661851491 1107 36-37 0.4174683322212971 1107 38-39 -0.3282776886398331 1107 40-41 0.4819509353901523 1107 42-43 0.6009317451995457 1107 44-45 0.7049371709046213 1107 46-47 0.8456442577861338 1107 48-49 0.4414459931169148 1107 50-51 1.3184872921159183 1107 52-53 0.37693157626119245 1107 54-55 0.6650239961362203 1107 56-57 0.7174098865093015 1107 58-59 0.7897917066181037 1107 60-61 0.21057110049546424 1107 62-63 1.3572268006774806 1107 64-65 2.2142375050851797 1107 66-67 2.3233041585738867 1107 68-69 3.1553619593520033 1107 70-71 2.3355742303605105 1107 72-73 -0.4601624647258298 1107 74-75 -0.032295746074773035 1107 76 0.7505406661615766 1108 1 -0.7435696172321542 1108 2 -0.060066129539706026 1108 3 0.48387866597754936 1108 4 0.20765159184451676 1108 5 -0.38688145552711006 1108 6 -0.4648329754719285 1108 7 -0.1912245346972199 1108 8 -0.666052069475235 1108 9 -0.06813884244566992 1108 10-11 -0.5450295244230858 1108 12-13 -0.9150127836092281 1108 14-15 -0.3641293433359394 1108 16-17 -0.20619445737038689 1108 18-19 0.33005950386008465 1108 20-21 0.11133738311065144 1108 22-23 -1.0331438365053742 1108 24-25 -0.9880721407596624 1108 26-27 0.3680027188721837 1108 28-29 0.5161787587739823 1108 30-31 -0.13379726230648714 1108 32-33 0.35003739311265036 1108 34-35 0.37019469695437834 1108 36-37 -0.361552646799673 1108 38-39 -0.0765294368915832 1108 40-41 -1.8180490646098448 1108 42-43 -0.629138184870385 1108 44-45 -0.4020558360883868 1108 46-47 -0.3054046932628225 1108 48-49 -0.27114141947049575 1108 50-51 -0.22067354704491748 1108 52-53 -0.2601313608017506 1108 54-55 -0.75036061924839 1108 56-57 0.06006722916664842 1108 58-59 -1.249369132542732 1108 60-61 -1.4663519764276174 1108 62-63 -0.7784375349868569 1108 64-65 0.627524218371903 1108 66-67 1.971555906825639 1108 68-69 1.8693479733380123 1108 70-71 1.7586511534374338 1108 72-73 0.9957815912182255 1108 74-75 -0.03858945236848044 1108 76 1.2442469598678727 1109 1 -0.14175143541397262 1109 2 0.09629750682393023 1109 3 0.4075150296139114 1109 4 0.10765159184452244 1109 5 0.05311854447288766 1109 6 -0.26483297547192564 1109 7 0.24877546530277783 1109 8 -0.2242338876570571 1109 9 0.10277024846342186 1109 10-11 -0.3223022516958167 1109 12-13 0.3768053982089583 1109 14-15 -0.03776570697230852 1109 16-17 -0.4725580937340226 1109 18-19 0.3218776856782739 1109 20-21 0.12679192856519705 1109 22-23 0.35140161804007874 1109 24-25 -0.02625395894148852 1109 26-27 -0.7392700084005455 1109 28-29 0.0607242133194319 1109 30-31 0.048020919511699844 1109 32-33 0.15276466583993198 1109 34-35 -0.70980530304562 1109 36-37 -0.687916283163311 1109 38-39 -2.342893073255219 1109 40-41 -1.4480490646098403 1109 42-43 0.006316360584158076 1109 44-45 -0.7866012906338327 1109 46-47 -0.0335865114446392 1109 48-49 -0.5093232376523105 1109 50-51 -0.8561280924994676 1109 52-53 -0.6592222698926591 1109 54-55 -0.36036061924838947 1109 56-57 -1.347205498106078 1109 58-59 -0.5548236779972839 1109 60-61 0.363648023572388 1109 62-63 -1.0566193531686707 1109 64-65 0.07116058200826103 1109 66-67 -0.17208045681072903 1109 68-69 -0.7115611175710796 1109 70-71 -0.441348846562569 1109 72-73 -1.2878547724181395 1109 74-75 -0.44768036145938694 1109 76 -0.38484394922303977 1110 1 -0.9981150717776117 1110 2 0.39447932500574723 1110 3 0.48387866597754936 1110 4 0.26219704638997143 1110 5 0.3585730899274324 1110 6 -0.010287520926468119 1110 7 0.17241182893914697 1110 8 0.333947930524765 1110 9 0.5318611575543315 1110 10-11 0.07315229375872434 1110 12-13 0.3395326709362294 1110 14-15 0.12677974757314558 1110 16-17 0.15744190626597288 1110 18-19 0.7209685947691824 1110 20-21 0.7477010194742917 1110 22-23 0.10321979985825891 1110 24-25 0.8210187683312355 1110 26-27 0.3043663552358211 1110 28-29 0.7889060315012557 1110 30-31 0.7389300104207877 1110 32-33 0.8864010294762963 1110 34-35 0.34292196968165456 1110 36-37 0.4929928077457788 1110 38-39 -2.249256709618855 1110 40-41 -0.3362308827916607 1110 42-43 0.6890436333114351 1110 44-45 0.8343078002752549 1110 46-47 0.5491407612826293 1110 48-49 0.5015858532567776 1110 50-51 -0.36612809249946565 1110 52-53 0.6853231846527947 1110 54-55 0.4769121080243366 1110 56-57 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38-39 1.4611810008188577 2116 40-41 -2.0069379534987277 2116 42-43 -0.4429428986750992 2116 44-45 0.3989542649217199 2116 46-47 -1.0717346595927921 2116 48-49 -0.6004343487634287 2116 50-51 -0.6179799443513119 2116 52-53 -0.15811115878154425 2116 54-55 0.36075049186272423 2116 56-57 -0.7672054981060796 2116 58-59 0.0051763220027183365 2116 60-61 -0.26635197642761455 2116 62-63 -0.6743971309464527 2116 64-65 -0.5792097883621139 2116 66-67 -0.8809693456996186 2116 68-69 -1.8067463027562631 2116 70-71 -0.8339414391551614 2116 72-73 1.3914044868411217 2116 74-75 0.9338011200220926 2116 76 3.8314523470732595 2117 1 0.08824856458602426 2117 2 0.5762975068239271 2117 3 0.4975150296139148 2117 4 0.4076515918445196 2117 5 0.4131185444728871 2117 6 0.13516702452807294 2117 7 0.35877546530277726 2117 8 -0.8342338876570565 2117 9 0.3727702484634179 2117 10-11 0.577697748304189 2117 12-13 -0.2831946017910454 2117 14-15 -0.6277657069723048 2117 16-17 0.2574419062659672 2117 18-19 0.11187768567827305 2117 20-21 0.2567919285651996 2117 22-23 0.42140161804007903 2117 24-25 -0.531253958941484 2117 26-27 0.4157299915994557 2117 28-29 -2.72927578668056 2117 30-31 -0.9519790804883002 2117 32-33 0.12276466583993084 2117 34-35 0.37019469695437834 2117 36-37 -2.072916283163316 2117 38-39 0.11210692674478295 2117 40-41 0.30695093539015517 2117 42-43 -1.8586836394158368 2117 44-45 -1.0566012906338358 2117 46-47 -0.42358651144463977 2117 48-49 -0.9643232376523159 2117 50-51 -1.91612809249947 2117 52-53 0.005777730107340062 2117 54-55 0.99963938075161 2117 56-57 -1.2922054981060782 2117 58-59 -2.544823677997279 2117 60-61 -0.7163519764276174 2117 62-63 -0.24661935316866845 2117 64-65 -1.45883941799174 2117 66-67 -3.1170804568107293 2117 68-69 -0.8215611175710791 2117 70-71 -1.3413488465625676 2117 72-73 1.3821452275818622 2117 74-75 1.2523196385406123 2117 76 1.6851560507769605 2118 1 0.4382485645860257 2118 2 -0.4763340721234428 2118 3 -0.24722181249134678 2118 4 0.14449369710767712 2118 5 -1.1658288239481678 2118 6 -0.05430665968245307 2118 7 0.33509125477646506 2118 8 -1.2421286244991592 2118 9 0.3490860379371057 2118 10-11 0.4803293272515532 2118 12-13 -0.7752998649489342 2118 14-15 0.27749745092243217 2118 16-17 0.025862958897548083 2118 18-19 0.6171408435730115 2118 20-21 0.17258140224940632 2118 22-23 0.30034898646113106 2118 24-25 0.10427235684799285 2118 26-27 -0.5382173768215992 2118 28-29 0.44967158174048905 2118 30-31 0.23223144582749455 2118 32-33 -3.024603755212702 2118 34-35 -1.0561210925193052 2118 36-37 -0.49791628316331327 2118 38-39 0.3607911372710966 2118 40-41 0.908266724863843 2118 42-43 0.52684267637364 2118 44-45 0.05392502515563535 2118 46-47 -0.7130601956551672 2118 48-49 -0.9366916587049445 2118 50-51 -0.7661280924994642 2118 52-53 -2.2165906909452886 2118 54-55 -0.4608869350378626 2118 56-57 -1.3724686560008195 2118 58-59 -1.2053499937867542 2118 60-61 1.0494374972565907 2118 62-63 -1.0545140900107839 2118 64-65 -1.8930499443075277 2118 66-67 -3.860501509442308 2118 68-69 -6.805771643886867 2118 70-71 -3.8729277939309874 2118 72-73 -3.1310126671549803 2118 74-75 -1.942417203564652 2118 76 -4.201686054486196 2119 1 0.3206015057624967 2119 2 -0.01193778729371786 2119 3 0.6475150296139134 2119 4 0.4076515918445196 2119 5 -0.17511674964475787 2119 6 0.4998729068810164 2119 7 0.4558342888321931 2119 8 -0.4371750641276435 2119 9 0.11688789552224677 2119 10-11 0.22181539536300932 2119 12-13 0.33151128056189805 2119 14-15 -0.44541276579583666 2119 16-17 0.48097131803068294 2119 18-19 1.1295247445018006 2119 20-21 1.003850752094614 2119 22-23 0.28022514745184424 2119 24-25 0.7760989822349842 2119 26-27 0.4230829327759267 2119 28-29 0.8119006839076661 2119 30-31 0.7539032724528738 2119 32-33 -4.8713529812188945 2119 34-35 -0.14745236186914923 2119 36-37 1.3550248933072808 2119 38-39 5.2694598679212525 2119 40-41 2.352539170684274 2119 42-43 1.6692575370547544 2119 44-45 -1.5977777612220692 2119 46-47 0.04700172384947621 2119 48-49 -1.6952055905934884 2119 50-51 -0.08965750426416719 2119 52-53 0.6425424359896894 2119 54-55 0.837874674869262 2119 56-57 -0.12014667457667372 2119 58-59 0.9757645572968343 2119 60-61 2.5865892000429795 2119 62-63 0.9563218233019128 2119 64-65 -2.679427653285856 2119 66-67 -6.227374574457787 2119 68-69 -5.974502294041667 2119 70-71 -3.829584140680211 2119 72-73 4.132145227581862 2119 74-75 0.48173140324649566 2119 76 2.77045016842402 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 2.0 22 1.0 23 4.0 24 13.0 25 32.0 26 72.0 27 123.0 28 223.0 29 368.0 30 596.0 31 820.0 32 1404.0 33 2377.0 34 3599.0 35 4293.0 36 2327.0 37 1431.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 8.650910324550493 5.852086396019451 16.340608390817597 69.15639488861245 2 4.6703607373063445 17.522334049530702 57.293904783444525 20.51340042971842 3 5.14531267669343 7.033812054732556 60.41501752798823 27.405857740585777 4 4.393305439330543 56.91507406988578 26.444645482302388 12.246975008481284 5 4.619473029514871 22.147461268800182 67.79373515775188 5.439330543933055 6 8.204229333936446 54.74951939387086 15.141920162840664 21.90433110935203 7 7.344792491236006 20.60952165554676 26.891326472916433 45.154359380300804 8 6.264842248105847 55.303629989822454 6.4740472690263475 31.95748049304535 9 5.201854574239512 16.459346375664367 3.2341965396358705 75.10460251046025 10-11 13.652041162501414 41.221870405970826 24.63813185570508 20.487956575822686 12-13 14.42666515888273 8.71876060160579 34.86656112179124 41.98801311772023 14-15 8.173131290286102 4.763654868257379 14.618907610539408 72.4443062309171 16-17 32.84236118964152 30.199027479362208 3.064570846997625 33.894040483998644 18-19 44.66244487164989 22.831618229107768 2.697048512948095 29.808888386294246 20-21 20.25048060612914 11.534547099400656 2.889290964604772 65.32568132986543 22-23 8.896867578875947 37.08017641072034 2.9712767160465905 51.05167929435712 24-25 32.7575483433224 30.979305665498135 9.651701911116136 26.61144408006333 26-27 37.41942779599683 43.49768178220061 4.562931131968789 14.519959289833768 28-29 12.249802103358588 36.562818048173696 10.986090693203664 40.20128915526405 30-31 13.018771909985299 22.712880244260997 29.941761845527537 34.326586000226165 32-33 7.53420784801538 28.963587017980323 16.9682234535791 46.5339816804252 34-35 7.172339703720457 10.892796562252629 36.93599457197784 44.998869162049075 36-37 38.06965961777677 23.15107995024313 18.489200497568696 20.2900599344114 38-39 13.465452900599345 23.20479475291191 34.665837385502655 28.66391496098609 40-41 13.598326359832635 5.230125523012552 36.50344905575031 44.6680990614045 42-43 39.17788080967997 3.9240076896980662 31.946172113536132 24.95193938708583 44-45 12.063213841456518 28.313355196200384 43.347845753703496 16.2755852086396 46-47 32.13841456519281 8.896867578875947 31.295940291756192 27.668777564175052 48-49 30.959516001357006 3.2341965396358705 14.110030532624677 51.696256926382446 50-51 23.23871989143956 3.5593124505258396 32.729277394549364 40.47269026348525 52-53 6.242225489087414 3.6610878661087867 70.73956801990275 19.35711862490105 54-55 12.487278073052131 10.624222548908742 43.25455162275246 33.63394775528667 56-57 31.22526291982359 17.9350899016171 18.921746013796223 31.91790116476309 58-59 11.438425873572317 21.008142033246635 49.45719778355762 18.09623430962343 60-61 30.018093407214746 41.67420558633948 21.03075879226507 7.276942214180708 62-63 10.262354404613818 78.81657808436051 4.153002374759697 6.768065136265973 64-65 4.59120208074183 83.5830600474952 4.071016623317878 7.754721248445098 66-67 4.93045346601832 80.6768065136266 4.919145086509103 9.47359493384598 68-69 4.834332240189981 78.91835349994346 5.529797580006785 10.717516679859775 70-71 5.49869953635644 72.46126880018093 7.115797806174375 14.92423385728825 72-73 6.768065136265973 59.117380979305665 10.779712767160465 23.334841117267896 74-75 7.356100870745222 52.81013230804026 13.70575596517019 26.12801085604433 76 6.677598100192242 53.782652945832865 12.75019789664141 26.789551057333483 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 0.5 20 0.5 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 1.0 27 2.5 28 1.5 29 0.0 30 1.0 31 2.5 32 1.5 33 0.0 34 3.0 35 7.0 36 9.5 37 11.0 38 21.0 39 190.5 40 1575.0 41 3429.0 42 4058.0 43 3759.5 44 2774.5 45 1555.0 46 1022.0 47 762.0 48 373.0 49 188.0 50 132.0 51 95.0 52 50.0 53 47.0 54 52.0 55 55.0 56 69.5 57 103.0 58 125.0 59 146.0 60 183.5 61 219.5 62 239.0 63 232.0 64 232.5 65 236.5 66 228.5 67 224.0 68 235.5 69 232.5 70 196.5 71 175.0 72 151.5 73 115.5 74 89.0 75 75.0 76 56.0 77 31.0 78 15.5 79 6.0 80 4.5 81 1.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 17686.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.614384258735722 #Duplication Level Percentage of deduplicated Percentage of total 1 81.92562698183914 16.069207282596405 2 11.646007494955319 4.568585321723397 3 3.0268088786393776 1.7810697727015719 4 0.6341885269530124 0.49756869840551843 5 0.403574517151917 0.39579328282257153 6 0.403574517151917 0.47495193938708585 7 0.2017872585759585 0.2770552979758001 8 0.17296050735082155 0.2714011082211919 9 0.0 0.0 >10 0.9801095416546555 4.1445210901277845 >50 0.23061400980109542 3.1946172113536133 >100 0.2594407610262323 11.557163858419088 >500 0.028826751225136928 5.156621056202646 >1k 0.057653502450273855 20.123261336650458 >5k 0.028826751225136928 31.488182743412867 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT 5569 31.488182743412867 No Hit CACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCTG 2190 12.38267556259188 RNA PCR Primer, Index 32 (96% over 26bp) TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG 1369 7.740585774058577 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC 912 5.156621056202646 No Hit ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT 465 2.6291982358927966 RNA PCR Primer, Index 42 (95% over 22bp) TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC 436 2.46522673300916 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT 223 1.2608843152776206 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT 220 1.2439217460137961 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT 198 1.1195295714124165 No Hit TCTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC 152 0.8594368427004411 No Hit CTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG 124 0.7011195295714123 No Hit CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG 124 0.7011195295714123 No Hit CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC 102 0.5767273549700328 RNA PCR Primer, Index 32 (95% over 21bp) CTTATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC 92 0.5201854574239512 No Hit TATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCT 85 0.480606129141694 RNA PCR Primer, Index 42 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC 81 0.45798937012326135 No Hit TCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCG 69 0.39013909306796335 No Hit CACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGTCTTCTG 68 0.38448490331335516 RNA PCR Primer, Index 42 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACATCACTCGAGCTCGTATGCCGT 63 0.3562139545403144 No Hit CAGCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGC 54 0.3053262467488409 No Hit ACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCT 53 0.2996720569942327 RNA PCR Primer, Index 42 (95% over 24bp) TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCG 48 0.2714011082211919 No Hit TCTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCG 44 0.24878434920275921 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCC 38 0.21485921067511027 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCC 38 0.21485921067511027 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCC 33 0.18658826190206945 No Hit CTTATTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG 31 0.17527988239285308 No Hit CACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGTCTTCTG 31 0.17527988239285308 RNA PCR Primer, Index 42 (95% over 23bp) TTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC 31 0.17527988239285308 RNA PCR Primer, Index 32 (95% over 21bp) CCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG 30 0.16962569263824495 No Hit CTCTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG 30 0.16962569263824495 No Hit TCTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT 26 0.1470089336198123 No Hit CTTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC 24 0.13570055411059595 No Hit ACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCTGC 22 0.12439217460137961 RNA PCR Primer, Index 32 (96% over 27bp) CTTATAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG 20 0.11308379509216329 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACCCGATCTCGTATGCCGT 19 0.10742960533755513 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGCTCTCGTATGCCGT 18 0.10177541558294696 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGACATCGTATGCCGT 18 0.10177541558294696 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGAAATCGTATGCCGT 18 0.10177541558294696 No Hit TCCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC 18 0.10177541558294696 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.005654189754608165 0.0 7 0.0 0.0 0.0 0.005654189754608165 0.0 8 0.0 0.0 0.0 0.005654189754608165 0.0 9 0.0 0.0 0.0 0.03957932828225715 0.0 10 0.0 0.0 0.0 0.04523351803686532 0.0 11 0.0 0.0 0.0 0.06219608730068981 0.0 12 0.0 0.0 0.0 0.06219608730068981 0.0 13 0.0 0.0 0.0 0.06219608730068981 0.0 14 0.0 0.0 0.0 0.06785027705529798 0.0 15 0.0 0.0 0.0 0.06785027705529798 0.0 16 0.0 0.0 0.0 0.07350446680990615 0.0 17 0.0 0.0 0.0 0.07350446680990615 0.0 18 0.0 0.0 0.0 0.0961212258283388 0.0 19 0.0 0.0 0.0 0.15266312337442045 0.0 20 0.0 0.0 0.0 0.18093407214746127 0.0 21 0.0 0.0 0.0 0.18658826190206942 0.0 22 0.0 0.0 0.0 0.21485921067511027 0.0 23 0.0 0.0 0.0 0.22616759018432658 0.0 24 0.0 0.0 0.0 0.23182177993893474 0.0 25 0.0 0.0 0.0 0.23182177993893474 0.0 26 0.0 0.0 0.0 0.23182177993893474 0.0 27 0.0 0.0 0.0 0.2714011082211919 0.0 28 0.0 0.0 0.0 0.31098043650344903 0.0 29 0.0 0.0 0.0 0.37883071355874703 0.0 30 0.0 0.0 0.0 0.45233518036865317 0.0 31 0.0 0.0 0.0 0.5088770779147348 0.0 32 0.0 0.0 0.0 0.5880357344792492 0.0 33 0.0 0.0 0.0 0.7124279090806287 0.0 34 0.0 0.0 0.0 0.8820536017188737 0.0 35 0.0 0.0 0.0 1.0742960533755512 0.0 36 0.0 0.0 0.0 1.300463643559878 0.0 37 0.0 0.0 0.0 1.549247992762637 0.0 38 0.0 0.0 0.0 1.7980323419653963 0.0 39 0.0 0.0 0.0 2.0128915526405065 0.0 40 0.0 0.0 0.0 2.3182177993893474 0.0 41 0.0 0.0 0.0 2.600927287119756 0.0 42 0.0 0.0 0.0 2.8384032568132986 0.0 43 0.0 0.0 0.0 3.1098043650344906 0.0 44 0.0 0.0 0.0 3.3642429039918578 0.0 45 0.0 0.0 0.0 3.5904104941761847 0.0 46 0.0 0.0 0.0 3.91269931018885 0.0 47 0.0 0.0 0.0 4.116250141354744 0.0 48 0.0 0.0 0.0 4.43853895736741 0.0 49 0.0 0.0 0.0 4.659052357797128 0.0 50 0.0 0.0 0.0 4.856948999208414 0.0 51 0.0 0.0 0.0 5.015266312337442 0.0 52 0.0 0.0 0.0 5.184892004975687 0.0 53 0.0 0.0 0.0 5.326246748840891 0.0 54 0.0 0.0 0.0 5.495872441479136 0.0 55 0.0 0.0 0.0 5.654189754608165 0.0 56 0.0 0.0 0.0 5.869048965283275 0.0 57 0.0 0.0 0.0 5.993441139884655 0.0 58 0.0 0.0 0.0 6.174375212032116 0.0 59 0.0 0.0 0.0 6.372271853443402 0.0 60 0.0 0.0 0.0 6.575822684609295 0.0 61 0.0 0.0 0.0 6.74544837724754 0.0 62 0.0 0.0 0.0 6.898111500621961 0.0 63 0.0 0.0 0.0 7.062083003505598 0.0 64 0.0 0.0 0.0 7.186475178106977 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGCAAA 15 0.0021886867 70.0 58 TGCAAAA 15 0.0021886867 70.0 59 ACACCAA 15 0.0021886867 70.0 70 TGCTTGC 15 0.0021886867 70.0 55 GCTTGCA 15 0.0021886867 70.0 56 CTTGCAA 15 0.0021886867 70.0 57 TCTTTAT 60 0.0 70.0 1 TTTATAC 70 0.0 65.0 3 CTTTATA 65 0.0 64.61539 2 ACTCGAA 35 2.8103932E-7 60.0 32 ACTCGTA 30 8.044723E-6 58.333332 38 AACTCGT 30 8.044723E-6 58.333332 37 CGAACTC 30 8.044723E-6 58.333332 35 GAACTCG 30 8.044723E-6 58.333332 36 TCTTAAT 30 8.044723E-6 58.333332 1 TGCGCAG 20 0.0068397284 52.500004 31 AAAACCT 20 0.0068397284 52.500004 67 CTCGAAC 35 2.0043413E-5 50.0 33 ACTCGAC 35 2.0043413E-5 50.0 32 CCTCGTA 35 2.0043413E-5 50.0 38 >>END_MODULE