FastQCFastQC Report
Wed 25 May 2016
SRR1780345_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780345_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68614
Sequences flagged as poor quality0
Sequence length50
%GC63

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGTGT404.1073145E-738.43048533
CTGTGTA301.2799358E-436.7074834
CTATACA250.002358221235.136441
CAAGCCG250.002358221235.136444
CAGTGTA451.0095282E-634.26031534
TATGCAG451.0329004E-634.1604330
AGTATGC451.0329004E-634.1604328
GTATGCA451.0329004E-634.1604329
AACGGCT401.8176323E-532.94041429
TGCAGTG651.7267666E-830.40653832
CGAAGTA651.7267666E-830.40653825
TGACGGT300.005636236629.4089813
ATGCAGT602.923407E-729.28036931
ACGAGAA554.8852235E-627.99024424
AAGTATG554.9411174E-627.94944227
ACGAAGT804.1818566E-927.4904224
TCTCGGT1150.026.7342543
GGCTGTG508.331197E-526.35233132
GACGAAG857.9780875E-925.87333723
CTCGGTG1450.025.74653244
Produced by FastQC (version 0.11.5)