##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780345_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 68614 Sequences flagged as poor quality 0 Sequence length 50 %GC 63 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.716209519923048 28.0 16.0 31.0 10.0 34.0 2 23.67744192147375 28.0 15.0 33.0 10.0 34.0 3 23.84998688314338 28.0 16.0 33.0 10.0 34.0 4 26.24142303320022 32.0 17.0 37.0 10.0 37.0 5 26.352289620194128 32.0 17.0 37.0 10.0 37.0 6 26.453070801877168 32.0 17.0 37.0 8.0 37.0 7 26.413661934882093 32.0 16.0 37.0 8.0 37.0 8 26.411665257819102 32.0 16.0 37.0 8.0 37.0 9 27.356384994316027 32.0 17.0 39.0 8.0 39.0 10 27.168085230419447 32.0 16.0 39.0 8.0 39.0 11 27.310126213309236 32.0 17.0 39.0 8.0 39.0 12 27.15149969394001 32.0 16.0 39.0 8.0 39.0 13 27.078322208295685 32.0 16.0 39.0 8.0 39.0 14 27.69437724079634 32.0 17.0 40.0 8.0 41.0 15 27.559317340484448 31.0 16.0 40.0 8.0 41.0 16 27.443874427959308 31.0 16.0 40.0 8.0 41.0 17 27.449587547730783 31.0 16.0 40.0 8.0 41.0 18 27.227387996618766 31.0 16.0 40.0 8.0 41.0 19 27.092342670592007 31.0 15.0 40.0 8.0 41.0 20 27.07695222549334 31.0 15.0 40.0 8.0 41.0 21 26.937622059637974 30.0 15.0 40.0 8.0 41.0 22 26.90851721223074 30.0 15.0 40.0 8.0 41.0 23 26.752572361325676 30.0 15.0 40.0 8.0 41.0 24 26.42115311743959 30.0 15.0 39.0 8.0 41.0 25 26.30228524790859 30.0 14.0 39.0 8.0 41.0 26 26.073454397061823 30.0 13.0 39.0 7.0 41.0 27 25.81005334188358 29.0 13.0 39.0 7.0 41.0 28 25.669411490366397 28.0 13.0 38.0 7.0 41.0 29 25.377328242049728 28.0 12.0 38.0 7.0 41.0 30 25.125601189261666 27.0 12.0 38.0 7.0 41.0 31 24.974975952429535 27.0 12.0 37.0 7.0 41.0 32 24.639359314425626 27.0 12.0 37.0 7.0 40.0 33 24.538723875593902 27.0 12.0 37.0 7.0 40.0 34 24.210394380155652 26.0 11.0 36.0 7.0 40.0 35 23.897746815518698 25.0 11.0 35.0 7.0 40.0 36 24.07106421430029 26.0 11.0 35.0 7.0 40.0 37 23.93858396245664 26.0 11.0 35.0 7.0 40.0 38 23.837190660798086 26.0 11.0 35.0 7.0 39.0 39 23.529133995977496 25.0 10.0 35.0 7.0 39.0 40 23.078803159705018 24.0 9.0 35.0 7.0 39.0 41 23.113927186871482 24.0 10.0 35.0 7.0 39.0 42 23.022867053371034 24.0 10.0 35.0 7.0 38.0 43 22.98235054070598 24.0 10.0 35.0 7.0 37.0 44 22.86826303669805 24.0 10.0 35.0 7.0 37.0 45 22.899247966887224 24.0 10.0 35.0 7.0 37.0 46 22.684801935465064 24.0 10.0 35.0 7.0 36.0 47 22.51566735651616 23.0 10.0 35.0 7.0 36.0 48 22.432054682717812 23.0 9.0 35.0 7.0 36.0 49 22.24627627014895 23.0 10.0 35.0 7.0 35.0 50 22.166686099046842 23.0 10.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3813710970243065 1101 2 -0.44025849603866263 1101 3 0.3828347374643002 1101 4 0.05074413292160074 1101 5 -0.07203339685008103 1101 6 0.3796308888170188 1101 7 -0.8206584134640345 1101 8 0.8226366901459023 1101 9 -0.11290617144487669 1101 10 0.8763642108184868 1101 11 0.6526979135681152 1101 12 0.492569877892695 1101 13 0.0473852911963597 1101 14 1.0816821813661548 1101 15 -0.19799449827099735 1101 16 0.9646236337684719 1101 17 1.3236142121841254 1101 18 0.28438468237439807 1101 19 0.24172383948741683 1101 20 -0.17003939842228633 1101 21 0.6706444120447728 1101 22 0.2984083283487493 1101 23 0.259166610459193 1101 24 0.8522639731884176 1101 25 0.6767826863461792 1101 26 0.651224273654023 1101 27 1.0702589000793417 1101 28 -0.4342336088795804 1101 29 0.2586565191491843 1101 30 0.35578066368955774 1101 31 -0.11947599077072013 1101 32 0.9204081139400628 1101 33 0.627787417065214 1101 34 0.7541901747991382 1101 35 0.8785345418118773 1101 36 0.8322079776042379 1101 37 0.5606408931485625 1101 38 0.9696164603463231 1101 39 1.0412396853533856 1101 40 0.6743135235426472 1101 41 0.5305519744180067 1101 42 1.379106608469101 1101 43 0.6888408275073914 1101 44 0.16719467325976467 1101 45 -0.17617724462174777 1101 46 -0.0310761298872535 1101 47 0.6966974532434023 1101 48 -0.2398771951598313 1101 49 -0.8753926851377472 1101 50 -0.28454317899171144 1102 1 0.1615415243349183 1102 2 -0.6125885931260413 1102 3 1.0354560966876 1102 4 -0.32750829426286643 1102 5 -0.4020333968500793 1102 6 0.6240969082344918 1102 7 0.9662347904194633 1102 8 0.7565201852915351 1102 9 -0.015139181153614345 1102 10 1.3471409098476101 1102 11 1.0604649038593763 1102 12 0.5124727905140567 1102 13 0.878841601875969 1102 14 0.23372101631760955 1102 15 -0.39090711963022073 1102 16 0.6596721774577929 1102 17 0.7366239209219891 1102 18 0.38195749790838107 1102 19 0.8525976258951857 1102 20 0.8605431258495599 1102 21 -0.0025594714503753835 1102 22 -1.3776110891269795 1102 23 -0.5216100885699326 1102 24 -0.6710369976853698 1102 25 -0.661858090352851 1102 26 0.09190388530450733 1102 27 0.04375404571040775 1102 28 0.36547512898449597 1102 29 0.04962739293559082 1102 30 -0.1339280741745199 1102 31 -0.5893789033920811 1102 32 -0.13590256567158931 1102 33 0.1859427568710359 1102 34 0.3299183301389448 1102 35 0.34387434763711866 1102 36 -0.503811439871491 1102 37 -0.42246590296794295 1102 38 -0.24902431635270617 1102 39 -0.05244963503496436 1102 40 -0.6502495832534692 1102 41 0.7957946928646109 1102 42 0.33629107448852125 1102 43 -0.4160135414246504 1102 44 -0.23882474421596456 1102 45 0.20440527965009636 1102 46 -0.7440858386251179 1102 47 0.3639790066414612 1102 48 0.009054843675119884 1102 49 0.2706267323379805 1102 50 1.4068160443092594 1103 1 -0.2036352479677035 1103 2 -0.1525226469820602 1103 3 -0.32056148895079417 1103 4 -1.5334068104746272 1103 5 -0.052976793076496165 1103 6 -0.5509351489188319 1103 7 -1.2725452059168632 1103 8 -0.8530236872125876 1103 9 -0.9606420205014778 1103 10 -0.9351452231437776 1103 11 -1.620886992092263 1103 12 -1.563090499465794 1103 13 -1.383935463520622 1103 14 -0.5285064978791283 1103 15 -0.9691265737426953 1103 16 -0.9676405171749245 1103 17 -0.28883861800455435 1103 18 -0.6014643742293728 1103 19 -0.9201629529654127 1103 20 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0.5122523174356353 1104 6 -0.8746548254686957 1104 7 -0.3944679372735571 1104 8 -0.675934738425525 1104 9 -0.2324299809686856 1104 10 -0.8174453129910404 1104 11 -0.7949211340509308 1104 12 -0.4240967887739693 1104 13 -0.8059480421369756 1104 14 -0.41260353291956164 1104 15 -0.36418497446147313 1104 16 -0.12442398527914733 1104 17 -1.4773381687682559 1104 18 -1.1179962700065538 1104 19 -1.1087523509887731 1104 20 -0.4438489222318083 1104 21 -1.061736540336181 1104 22 0.4503130902535091 1104 23 -0.13797624668366382 1104 24 -0.6382122172877729 1104 25 0.3739255434890367 1104 26 -0.35210905967931083 1104 27 -1.1445030046825622 1104 28 -0.47280503745101043 1104 29 0.2915136620063272 1104 30 -0.009933622024728805 1104 31 -1.4337617050564369 1104 32 -0.3748299812980349 1104 33 -0.3422125829347884 1104 34 -0.20295268234371733 1104 35 -0.04956069628336124 1104 36 0.6479222633185238 1104 37 0.378736131243798 1104 38 0.5391402698701313 1104 39 0.2150492091629097 1104 40 0.07383733306645723 1104 41 0.5134091172751525 1104 42 -0.21470291534042119 1104 43 -0.15877822011165676 1104 44 0.627194673259762 1104 45 0.4333465649020596 1104 46 0.4698762510651271 1104 47 0.10193554848149589 1104 48 0.5163132810306443 1104 49 -0.37634506609012774 1104 50 0.8711711067225743 1105 1 -0.39877850443171425 1105 2 -0.49581405159421976 1105 3 -0.719757855128293 1105 4 -1.1025892004117317 1105 5 -0.7816630264797091 1105 6 -1.1344431852570551 1105 7 -0.452139894945514 1105 8 -1.0677336802244675 1105 9 -1.969202467741173 1105 10 -0.9232654188111447 1105 11 -1.430635419765217 1105 12 -0.5463190109961928 1105 13 -0.1744665606554925 1105 14 -1.4483178186338463 1105 15 -1.1824389427154394 1105 16 -0.19056155141671383 1105 17 -0.16305245448254269 1105 18 -1.2267264287367112 1105 19 -1.007165049401472 1105 20 -1.8330023613852475 1105 21 -0.8871333657330069 1105 22 -1.170850930910511 1105 23 -0.8437963525037695 1105 24 -1.38292121199677 1105 25 -1.1535876840241919 1105 26 -1.3391460967163482 1105 27 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13 -0.02588573684102613 1106 14 0.7596261065998 1106 15 -0.2636954328504366 1106 16 -1.1035072073530223 1106 17 -0.3935820494981179 1106 18 -1.1693536353826097 1106 19 0.10602290490797728 1106 20 -0.12003939842228561 1106 21 -1.4364583916935452 1106 22 -0.023741204361531487 1106 23 -0.13316983813894012 1106 24 -0.4757734099891522 1106 25 0.5205210041031876 1106 26 -0.5645701188693408 1106 27 -0.6902083896402864 1106 28 -0.7657289359823842 1106 29 -0.9990070322526847 1106 30 -0.34851840173100257 1106 31 0.005757654089091346 1106 32 -0.44594702624685567 1106 33 -0.28987613433665516 1106 34 -0.22992197473357123 1106 35 -0.7366991030479362 1106 36 -0.33685744295651077 1106 37 0.08456612679341902 1106 38 -0.1787947546069475 1106 39 -0.33119022118867036 1106 40 -0.31325656991529627 1106 41 -0.14159755829227194 1106 42 -0.5114541391944485 1106 43 0.5374389583485097 1106 44 -0.8008427099178057 1106 45 0.20513116659320474 1106 46 -0.16528173736389107 1106 47 -0.3185361916164098 1106 48 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3 0.22468658931614982 2214 4 1.0362996884771576 2214 5 0.12574438092769924 2214 6 0.5322234814096092 2214 7 0.7052675124618943 2214 8 0.10819224570145991 2214 9 -0.11735061588931828 2214 10 0.5767345811888553 2214 11 1.597142358012558 2214 12 0.6573846927075095 2214 13 -0.8966887828777139 2214 14 0.05168218136615366 2214 15 -0.5898463501228477 2214 16 0.5501791893240302 2214 17 -0.005645047075134357 2214 18 0.12512542311513997 2214 19 0.1965386543022305 2214 20 -0.5274468058296939 2214 21 -0.3686148472144879 2214 22 -0.031962042021621784 2214 23 -0.1400926488000671 2214 24 0.5985602694847145 2214 25 0.09641231597580813 2214 26 -0.5428498004200506 2214 27 -0.18233369251325016 2214 28 0.1890997244537509 2214 29 -0.34393607344340893 2214 30 0.02392881183770612 2214 31 0.7934869721922411 2214 32 -0.040702997171049304 2214 33 0.4114911207689147 2214 34 0.6597457303546967 2214 35 0.8829789862563189 2214 36 0.08257834797460717 2214 37 0.3536038561115227 2214 38 0.24998683071669348 2214 39 0.3893878335015337 2214 40 0.5309801902093163 2214 41 0.9798112336772675 2214 42 0.5850325343950296 2214 43 0.36661860528516854 2214 44 0.1901576362227253 2214 45 -0.22988094832545158 2214 46 0.7481831293720056 2214 47 0.930771527317475 2214 48 0.40864132335868675 2214 49 0.39719990745484424 2214 50 0.7206420061934757 2215 1 1.5109653515738266 2215 2 1.3097415039613374 2215 3 1.3751711860624312 2215 4 1.983080581519733 2215 5 1.9811441732433792 2215 6 1.7293505149852422 2215 7 1.0900892500873702 2215 8 2.029272204164595 2215 9 1.2126078472467121 2215 10 1.9960838369867098 2215 11 1.2321371659045646 2215 12 1.337896980696435 2215 13 2.076918001476731 2215 14 1.6287849851044705 2215 15 2.1755569035981637 2215 16 1.2796703627404362 2215 17 1.8867917822775837 2215 18 1.0736370188229962 2215 19 0.9191070170575095 2215 20 1.6930447137272466 2215 21 1.479429458773744 2215 22 1.611772814330056 2215 23 0.8668301618610599 2215 24 1.6644135058986969 2215 25 1.156035022794775 2215 26 0.23916819888766838 2215 27 1.5060532926027044 2215 28 1.935205643456868 2215 29 1.430899509803389 2215 30 0.6421358038764744 2215 31 1.8468791494161962 2215 32 1.0119968989867907 2215 33 1.0278808750091386 2215 34 1.9663397075094196 2215 35 1.431525196017482 2215 36 0.7752920897537692 2215 37 0.9911081828681887 2215 38 1.2511117874491262 2215 39 0.9678751993720773 2215 40 2.041883617000593 2215 41 1.7555987033899711 2215 42 2.899760814076579 2215 43 2.3785604536756146 2215 44 1.9468208414840618 2215 45 1.906065746032457 2215 46 1.591727608430503 2215 47 1.5132395093181685 2215 48 1.7429265431579246 2215 49 1.7018035765444957 2215 50 1.5331203724101563 2216 1 -1.4140983697515779 2216 2 -1.135713041493208 2216 3 -0.4862561716266107 2216 4 0.4125623147397839 2216 5 -1.5111243059409887 2216 6 -1.2694600202738933 2216 7 -1.3615675043731237 2216 8 -0.6473633098540965 2216 9 -0.16836071689942145 2216 10 -0.7754539709996955 2216 11 -0.20184754097734015 2216 12 -0.5665210311982136 2216 13 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2216 49 -2.07721086695593 2216 50 -1.4963613608098925 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 3.0 6 16.0 7 415.0 8 1311.0 9 2330.0 10 2299.0 11 2241.0 12 2320.0 13 2514.0 14 2558.0 15 2607.0 16 2490.0 17 2245.0 18 2198.0 19 1890.0 20 1683.0 21 1555.0 22 1453.0 23 1371.0 24 1280.0 25 1194.0 26 1223.0 27 1176.0 28 1150.0 29 1166.0 30 1194.0 31 1174.0 32 1268.0 33 1431.0 34 1705.0 35 2342.0 36 3959.0 37 6153.0 38 5477.0 39 3223.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.74261812458099 21.249307721456262 9.519923047774506 29.488151106188244 2 30.38972774441789 14.736489243864048 28.423599370372532 26.450183641345536 3 31.59850759320255 15.298627102340632 23.136677645961466 29.966187658495354 4 29.49835310578016 15.151426822514354 20.284490045763256 35.06573002594223 5 28.22164572827703 24.280759028769637 26.593698079109217 20.903897163844114 6 39.72658641093654 22.68050252135133 12.462471215786866 25.130439851925264 7 36.0567814148716 22.881627656163463 12.944880053633371 28.116710875331563 8 30.603083918733788 28.6282682834407 14.94301454513656 25.825633252688956 9 29.5978896127556 15.591797472782126 13.25259061693848 41.55772229752379 10 28.083190019529543 21.38193371615122 20.415658611945084 30.119217652374154 11 40.021570256365415 17.903312783291796 17.458790608193784 24.61632635214901 12 28.277440135251336 18.02136621339979 19.82277410986256 33.87841954148631 13 34.968591957792256 13.987145293166018 19.31266669581566 31.731596053226063 14 34.26368565265551 15.125633999883403 16.67201072698653 33.938669620474556 15 33.34110651198041 19.546143531743716 15.43607532210109 31.676674634174777 16 35.034058256392306 16.78845957496463 15.776192768272582 32.40128940037049 17 37.37101381682504 17.660176062496355 15.957849938786218 29.010960181892383 18 34.665499379878895 15.517618734952944 15.453417961625446 34.36346392354272 19 33.910327514741084 16.175783758538152 18.25413042209119 31.659758304629577 20 36.22892181911088 16.89612380465727 18.043652821373822 28.83130155485802 21 32.45244982031729 17.15166389108014 15.416191895287346 34.97969439331522 22 35.709704690551845 15.294580648922834 14.66198291694604 34.33373174357928 23 38.570741447915 16.815578131148975 15.5169146904925 29.09676573044353 24 33.05768249106687 21.478888645810546 14.53365419674761 30.92977466637497 25 36.393715018656714 17.127740205223883 14.406483208955223 32.07206156716418 26 35.15006632363016 19.819832951911724 15.697564246461527 29.33253647799659 27 33.47667638483965 19.75072886297376 16.3265306122449 30.446064139941694 28 37.48761005189202 18.710279284006763 15.322721707189086 28.479388956912132 29 33.54617683504629 19.345433340622495 17.800131204898317 29.308258619432902 30 35.3370188310457 17.648088451945096 16.185072275624663 30.82982044138454 31 35.02981353782456 17.35308267607482 17.532401265435247 30.084702520665374 32 32.93289207038824 18.33768278637139 18.578239127582336 30.151186015658034 33 35.69564520126548 16.225160735373446 18.267702759837295 29.811491303523784 34 34.60708558098848 17.689167517130777 18.126549059629685 29.577197842251056 35 33.26967930029154 20.067055393586006 17.590379008746357 29.072886297376094 36 36.25297041972213 16.28591838817372 18.293411864184392 29.16769932791976 37 33.54615821926794 16.562440780746638 19.262109881780148 30.629291118205277 38 33.44218797836514 16.49876809586984 21.36369600396542 28.695347921799602 39 33.649696757639376 15.45223932820154 21.587065547002567 29.31099836715652 40 32.12085826886586 21.69464311779302 20.328419208874617 25.856079404466502 41 33.2560648472125 15.084849545136459 22.442850478189875 29.21623512946116 42 29.140607916028866 17.507106931992126 23.85742401049639 29.494861141482616 43 30.27157830289071 14.710126969780902 23.43328619950728 31.585008527821106 44 28.19305559604676 16.23422057665957 25.391388006180577 30.181335821113088 45 28.32883900590336 14.433350338896581 27.646672983018732 29.59113767218133 46 28.459823055284296 16.939468582838987 23.905027037269164 30.69568132460756 47 29.887620796711705 16.72424096666521 26.60952963983267 26.778608596790416 48 30.559118470149254 18.20050139925373 22.69851912313433 28.54186100746269 49 29.986298973880597 17.706389925373134 23.34421641791045 28.963094682835823 50 30.23160901948781 20.293847566574836 21.34330316148498 28.131240252452372 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 1.5 10 2.0 11 1.0 12 0.0 13 2.0 14 4.0 15 3.5 16 3.0 17 9.5 18 16.0 19 13.0 20 10.0 21 22.0 22 34.0 23 43.0 24 52.0 25 68.0 26 84.0 27 107.0 28 130.0 29 168.0 30 206.0 31 239.5 32 273.0 33 347.0 34 421.0 35 497.0 36 573.0 37 647.0 38 721.0 39 829.5 40 938.0 41 1082.5 42 1227.0 43 1321.5 44 1416.0 45 1602.0 46 1788.0 47 1983.5 48 2179.0 49 2365.0 50 2551.0 51 2676.5 52 2802.0 53 2910.0 54 3018.0 55 3042.5 56 3067.0 57 3184.0 58 3301.0 59 3294.0 60 3287.0 61 3410.0 62 3533.0 63 3545.0 64 3557.0 65 3747.5 66 3938.0 67 3963.0 68 3988.0 69 4067.5 70 4147.0 71 4167.0 72 4187.0 73 4111.5 74 4036.0 75 3839.0 76 3642.0 77 3295.0 78 2948.0 79 2681.0 80 2414.0 81 2006.5 82 1599.0 83 1351.5 84 1104.0 85 886.0 86 668.0 87 524.0 88 380.0 89 277.5 90 175.0 91 132.0 92 89.0 93 68.5 94 48.0 95 38.5 96 29.0 97 23.0 98 17.0 99 14.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.002914857026262862 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.001457428513131431 10 0.0 11 0.001457428513131431 12 0.001457428513131431 13 0.001457428513131431 14 0.002914857026262862 15 0.002914857026262862 16 0.0801585682222287 17 0.002914857026262862 18 0.11513685253738304 19 0.14282799428688023 20 0.1734339930626403 21 0.23318856210102892 22 0.011659428105051448 23 0.007287142565657154 24 0.07141399714344011 25 0.008744571078788585 26 0.01603171364444574 27 0.020403999183840033 28 0.014574285131314308 29 0.02769114174949719 30 0.08307342524849157 31 0.03060599877576005 32 0.033520855802022916 33 0.033520855802022916 34 0.03497828431515434 35 0.020403999183840033 36 0.03060599877576005 37 0.018946570670708603 38 0.03060599877576005 39 0.03206342728889148 40 0.15157256536566882 41 0.03206342728889148 42 0.02769114174949719 43 0.021861427696971462 44 0.01748914215757717 45 0.013116856618182878 46 0.007287142565657154 47 0.010201999591920016 48 0.008744571078788585 49 0.008744571078788585 50 0.010201999591920016 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 68614.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.35470895152592 #Duplication Level Percentage of deduplicated Percentage of total 1 88.77135313762173 73.99510304019587 2 6.14585701047331 10.245722447313959 3 2.9846309863095133 7.463491415746058 4 1.304355428111832 4.34896668318419 5 0.4965642648575875 2.0695484886466318 6 0.1888342979035896 0.9444136765091673 7 0.0629447659678632 0.3672719853091206 8 0.013987725770636266 0.09327542484041158 9 0.0104907943279772 0.07870113970909727 >10 0.019233122934624865 0.30897484478386333 >50 0.0017484657213295333 0.08453085376162299 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004372285539394292 0.0 2 0.0 0.0 0.0 0.011659428105051448 0.0 3 0.0 0.0 0.0 0.011659428105051448 0.0 4 0.0 0.0 0.0 0.026233713236365756 0.0 5 0.0 0.0 0.0 0.036435712828285775 0.0 6 0.0 0.0 0.0 0.036435712828285775 0.0 7 0.0 0.0 0.0 0.037893141341417205 0.0 8 0.0 0.0 0.0 0.037893141341417205 0.0 9 0.0 0.0 0.0 0.07870113970909727 0.0 10 0.0 0.0 0.0 0.09619028186667444 0.0 11 0.0 0.0 0.0 0.11076456699798874 0.0 12 0.0 0.0 0.0 0.11222199551112018 0.0 13 0.0 0.0 0.0 0.11222199551112018 0.0 14 0.0 0.0 0.0 0.11659428105051448 0.0 15 0.0 0.0 0.0 0.11950913807677734 0.0 16 0.0 0.0 0.0 0.12825370915556591 0.0 17 0.0 0.0 0.0 0.13262599469496023 0.0 18 0.0 0.0 0.0 0.14865770833940595 0.0 19 0.0 0.0 0.0 0.17780627860203457 0.0 20 0.0 0.0 0.0 0.19383799224648032 0.0 21 0.0 0.0 0.0 0.20112513481213745 0.0 22 0.0 0.0 0.0 0.2171568484565832 0.0 23 0.0 0.0 0.0 0.22881627656163464 0.0 24 0.0 0.0 0.0 0.2448479902060804 0.0 25 0.0 0.0 0.0 0.26525198938992045 0.0 26 0.0 0.0 0.0 0.2798262745212347 0.0 27 0.0 0.0 0.0 0.3526977001778063 0.0 28 0.0 0.0 0.0 0.432856268400035 0.0 29 0.0 0.0 0.0 0.5538228349899438 0.0 30 0.0 0.0 0.0 0.6791616871192468 0.0 31 0.0 0.0 0.0 0.8351065380243099 0.0 32 0.0 0.0 0.0 1.0391465298627103 0.0 33 0.0 0.0 0.0 1.2898242341213164 0.0 34 0.0 0.0 0.0 1.6512665053779112 0.0 35 0.0 0.0 0.0 1.9937622059637974 0.0 36 0.0 0.0 0.0 2.540297898388084 0.0 37 0.0 0.0 0.0 3.229661585099251 0.0 38 0.0 0.0 0.0 3.868015273850818 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCAGTGT 40 4.1073145E-7 38.430485 33 CTGTGTA 30 1.2799358E-4 36.70748 34 CTATACA 25 0.0023582212 35.13644 1 CAAGCCG 25 0.0023582212 35.13644 4 CAGTGTA 45 1.0095282E-6 34.260315 34 TATGCAG 45 1.0329004E-6 34.16043 30 AGTATGC 45 1.0329004E-6 34.16043 28 GTATGCA 45 1.0329004E-6 34.16043 29 AACGGCT 40 1.8176323E-5 32.940414 29 TGCAGTG 65 1.7267666E-8 30.406538 32 CGAAGTA 65 1.7267666E-8 30.406538 25 TGACGGT 30 0.0056362366 29.40898 13 ATGCAGT 60 2.923407E-7 29.280369 31 ACGAGAA 55 4.8852235E-6 27.990244 24 AAGTATG 55 4.9411174E-6 27.949442 27 ACGAAGT 80 4.1818566E-9 27.49042 24 TCTCGGT 115 0.0 26.73425 43 GGCTGTG 50 8.331197E-5 26.352331 32 GACGAAG 85 7.9780875E-9 25.873337 23 CTCGGTG 145 0.0 25.746532 44 >>END_MODULE