FastQCFastQC Report
Wed 25 May 2016
SRR1780345_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780345_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68614
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT2148431.31139417611566No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG55978.157227387996619No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC47146.870318010901564No Hit
CACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTTCTG33094.822630949951905Illumina PCR Primer Index 4 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC11861.728510216573877No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTT7011.0216573877051331RNA PCR Primer, Index 27 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC3060.44597312501821784No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG2660.38767598449296065No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG2560.37310169936164633No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCATCTCGTATGCCGTCT2080.30314513073133764RNA PCR Primer, Index 20 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC1730.25213513277173755No Hit
TATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCT1670.24339056169294898No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCATCTCGTATGCCGTC1570.22881627656163467RNA PCR Primer, Index 4 (95% over 21bp)
CTTATATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC1360.1982102777858746No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC1350.1967528492727432No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCGT1260.1836359926545603No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGC1200.1748914215757717No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG1100.1603171364444574No Hit
TCTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT740.10784970997172588No Hit
CTCTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG730.10639228145859446No Hit
TTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC690.10056256740606873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTAAT302.4564379E-644.094231
TCTTTAT1200.042.2569731
TCTTCTG3300.039.9980144
GTCTTCT3500.037.7124143
CTATATC301.2887575E-436.6648535
TATATCG301.2887575E-436.6648536
CGTCTTC3600.036.66484542
CCTATAT250.002337975235.19825434
TATCGTA250.002337975235.19825438
ATCGTAT250.002337975235.19825439
ATATCGT250.002337975235.19825437
CCCTATA250.002337975235.19825433
ATGCCGT28400.034.93488744
TTTATAC1750.031.427013
CCGTCTT4300.030.6961541
CTTTATA1650.030.665142
TATGCCG34950.028.38771843
CTTATAC36250.028.0986671
GCCGTCT4700.028.0837140
CCCATCT406.966311E-427.49863433
Produced by FastQC (version 0.11.5)