FastQCFastQC Report
Wed 25 May 2016
SRR1780344_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780344_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95836
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACC200.006932697752.524
TTCAGCA350.001250016540.018
CCTATGA400.002408417435.014
ATCGAAG400.002408417435.08
TAACATA552.9731626E-431.818181
CTTATAA450.00428896431.1111131
TGAATGG450.00428896431.1111135
TTTGTGG500.007177937328.00000252
TATCGAA500.007177937328.0000027
AACATCT657.905914E-426.9230777
ATATAGG851.2968236E-424.7058831
CTGGCCC750.00182085123.33333446
GCAGTCC1703.6507117E-922.6470670
CGCCATC800.00264951421.87518
AAACTCT850.003765829820.58823636
CCCAGCT900.005242330519.44444550
CTCCAGC1000.009627596517.50000259
ATAGTAG1401.8432563E-417.53
AGCAGTC2401.9059735E-716.04166669
TACACAA1550.00730976713.5483875
Produced by FastQC (version 0.11.5)