##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780344_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 95836 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.011217079176927 34.0 31.0 34.0 12.0 34.0 2 30.209388956133395 34.0 32.0 34.0 12.0 34.0 3 30.05964355774448 34.0 31.0 34.0 11.0 34.0 4 30.06174089068826 34.0 31.0 34.0 11.0 34.0 5 30.018542092741768 34.0 31.0 34.0 11.0 34.0 6 32.73555866271547 38.0 34.0 38.0 11.0 38.0 7 33.21594181727117 38.0 34.0 38.0 12.0 38.0 8 32.98052923744731 38.0 34.0 38.0 11.0 38.0 9 33.27576276138403 38.0 34.0 38.0 12.0 38.0 10-11 33.064704286489416 38.0 34.0 38.0 11.0 38.0 12-13 33.16742664551943 38.0 34.0 38.0 11.0 38.0 14-15 33.05392545598731 38.0 34.0 38.0 11.0 38.0 16-17 33.09036270295087 38.0 34.0 38.0 11.0 38.0 18-19 33.112431654075706 38.0 34.0 38.0 11.0 38.0 20-21 33.10521620267957 38.0 34.0 38.0 11.0 38.0 22-23 33.08206728160607 38.0 34.0 38.0 11.0 38.0 24-25 33.11125255645061 38.0 34.0 38.0 11.0 38.0 26-27 33.07206582077716 38.0 34.0 38.0 11.0 38.0 28-29 33.12356004006845 38.0 34.0 38.0 11.0 38.0 30-31 33.15020451604825 38.0 34.0 38.0 11.0 38.0 32-33 33.19117033265161 38.0 34.0 38.0 11.0 38.0 34-35 33.19428502859051 38.0 34.0 38.0 11.0 38.0 36-37 33.11873930464544 38.0 34.0 38.0 11.0 38.0 38-39 33.09671209148963 38.0 34.0 38.0 11.0 38.0 40-41 33.142389081347304 38.0 34.0 38.0 11.0 38.0 42-43 33.21071413665011 38.0 34.0 38.0 11.0 38.0 44-45 33.27808965315748 38.0 34.0 38.0 11.0 38.0 46-47 33.18405400893192 38.0 34.0 38.0 11.0 38.0 48-49 33.14110042155349 38.0 34.0 38.0 11.0 38.0 50-51 33.090999206978594 38.0 34.0 38.0 11.0 38.0 52-53 33.175982929170665 38.0 34.0 38.0 11.0 38.0 54-55 33.13794920489169 38.0 34.0 38.0 11.0 38.0 56-57 33.07746567052047 38.0 34.0 38.0 11.0 38.0 58-59 33.14260298843858 38.0 34.0 38.0 11.0 38.0 60-61 33.15461308902709 38.0 34.0 38.0 11.0 38.0 62-63 33.029258316290324 38.0 34.0 38.0 11.0 38.0 64-65 33.00221211235861 38.0 34.0 38.0 11.0 38.0 66-67 32.95780813055637 38.0 33.5 38.0 11.0 38.0 68-69 32.904863516841274 38.0 33.0 38.0 11.0 38.0 70-71 32.86145602904962 38.0 33.5 38.0 11.0 38.0 72-73 32.88933177511582 38.0 34.0 38.0 11.0 38.0 74-75 32.85562314787762 38.0 34.0 38.0 11.0 38.0 76 31.66525105388372 37.0 28.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2985127704995101 1101 2 -0.5543354852333557 1101 3 -0.461678656247976 1101 4 -0.43416359619500966 1101 5 -0.5465155415630605 1101 6 -0.46716582428914677 1101 7 -1.1823476557852857 1101 8 -1.0263258023689872 1101 9 -0.987310843798987 1101 10-11 -0.9015565219823003 1101 12-13 -0.6966957122481148 1101 14-15 -0.9516154485929817 1101 16-17 -1.0215453184427759 1101 18-19 -1.3793700911295375 1101 20-21 -1.5522081630243747 1101 22-23 -1.000919279071688 1101 24-25 -1.1997379237536947 1101 26-27 -1.5629967140891026 1101 28-29 -1.1688502645221277 1101 30-31 -1.3417573813506678 1101 32-33 -1.20922966903953 1101 34-35 -1.12968446402445 1101 36-37 -1.2301720943896939 1101 38-39 -1.0977037033577872 1101 40-41 -0.7111830589545605 1101 42-43 -1.5492776242934596 1101 44-45 -1.0297627901043924 1101 46-47 -0.7162620208150372 1101 48-49 -1.0707828730862303 1101 50-51 -0.8640456454464953 1101 52-53 -0.8956296180911494 1101 54-55 -1.1492750567461982 1101 56-57 -1.166949271126981 1101 58-59 -0.8993738601948564 1101 60-61 -1.2374879908828973 1101 62-63 -0.967206655443313 1101 64-65 -0.9731124726210112 1101 66-67 -1.435602541524176 1101 68-69 -1.5294505391764979 1101 70-71 -1.1573710134061912 1101 72-73 -0.7850411251126133 1101 74-75 -1.5308579198279162 1101 76 -2.856446089415037 1102 1 -0.7093886216328009 1102 2 -0.5712881948686501 1102 3 -0.3997907726550274 1102 4 -0.03930770588626942 1102 5 -0.2398743700305559 1102 6 -0.797032441209204 1102 7 -0.9592574589752871 1102 8 -1.0107022597889994 1102 9 -0.8813039414577872 1102 10-11 -0.9033054260024471 1102 12-13 -0.4903833346713995 1102 14-15 -1.1790079492925472 1102 16-17 -0.9064324558366721 1102 18-19 -0.675762685103976 1102 20-21 -1.254486401994619 1102 22-23 -0.7501264425825553 1102 24-25 -0.9627964071041291 1102 26-27 -1.2151630232020594 1102 28-29 -1.0270024891280443 1102 30-31 -0.2625786666785288 1102 32-33 -0.9128837125056322 1102 34-35 -0.9256922991144592 1102 36-37 -0.8418687644764447 1102 38-39 -0.5317700217982306 1102 40-41 -0.8459652621072493 1102 42-43 -1.3317722609878047 1102 44-45 -0.4278250044500709 1102 46-47 -1.1294930626954596 1102 48-49 -0.9437191663424542 1102 50-51 -0.9695395359417844 1102 52-53 -0.6131000033164042 1102 54-55 -0.6624734524182401 1102 56-57 -0.6027878123078381 1102 58-59 -0.7416693550180966 1102 60-61 -1.0097806874597097 1102 62-63 -0.9720919273395907 1102 64-65 -0.9528018672670342 1102 66-67 -0.9836974020782279 1102 68-69 -1.1734319774751611 1102 70-71 -0.406962935801495 1102 72-73 -0.5213996737554609 1102 74-75 -0.9779011060978533 1102 76 -0.5938539804699765 1103 1 -0.7319356763557003 1103 2 -0.49373504045944827 1103 3 -0.829404749650493 1103 4 -0.444958629553053 1103 5 -0.2785855934533501 1103 6 -0.9197232742520782 1103 7 -0.3176793829906259 1103 8 -0.7649284710124249 1103 9 -0.6480780787878686 1103 10-11 -0.1260746835834894 1103 12-13 -0.7229883364141685 1103 14-15 -0.9624799778739259 1103 16-17 -0.06882571132639015 1103 18-19 -0.33609729646682496 1103 20-21 -0.5770182075017658 1103 22-23 -0.992185958093188 1103 24-25 -0.6553068637092139 1103 26-27 -0.8108191751713818 1103 28-29 -0.7608165358342163 1103 30-31 -0.7448568995122642 1103 32-33 -0.779235228713361 1103 34-35 -0.3370834632831645 1103 36-37 -0.5248811381257994 1103 38-39 -0.9925795373088633 1103 40-41 -0.46393973056316185 1103 42-43 -0.7518119089487669 1103 44-45 -0.834641403892384 1103 46-47 -0.4289658755222234 1103 48-49 -0.6682856529231458 1103 50-51 -0.8532969427037216 1103 52-53 -0.4913023386248767 1103 54-55 -0.5699107127877028 1103 56-57 -0.42799912286901076 1103 58-59 -0.5140421329895233 1103 60-61 -0.7196970346190028 1103 62-63 -0.514996685094907 1103 64-65 -0.620466994489064 1103 66-67 -0.8943914592410067 1103 68-69 -0.68764642501786 1103 70-71 -0.4654001090325792 1103 72-73 -0.6071547277812499 1103 74-75 -1.004750618122948 1103 76 -0.4247837469391271 1104 1 0.40830955882527675 1104 2 0.7001799796489614 1104 3 0.988253494797835 1104 4 1.100755219021874 1104 5 0.44080372286068936 1104 6 1.0672499728190843 1104 7 0.5373542767400963 1104 8 0.2688405445497395 1104 9 0.728495622908234 1104 10-11 0.16101659111488686 1104 12-13 0.3081414502618287 1104 14-15 0.7349048359047003 1104 16-17 0.5651083243441732 1104 18-19 0.965834859504497 1104 20-21 0.22067916091928907 1104 22-23 0.784900289502545 1104 24-25 0.48989990320806953 1104 26-27 0.3912812794535512 1104 28-29 0.28126905605248 1104 30-31 0.49840569010130054 1104 32-33 0.6782638970079091 1104 34-35 0.44982749142919687 1104 36-37 -0.019384110673449584 1104 38-39 0.3927915940078037 1104 40-41 0.7235766621628414 1104 42-43 0.7167195213712176 1104 44-45 0.9379095228430572 1104 46-47 0.8647589889645673 1104 48-49 0.5226732122630793 1104 50-51 0.348836297373218 1104 52-53 0.5438327374373699 1104 54-55 0.5258337355424203 1104 56-57 0.8297109225934847 1104 58-59 0.5694624132598278 1104 60-61 0.8271205908399963 1104 62-63 0.3244912061448204 1104 64-65 0.617746635516184 1104 66-67 0.35077268658868377 1104 68-69 0.5289288066660021 1104 70-71 0.54032095234178 1104 72-73 0.5086161674801559 1104 74-75 0.6253584481473808 1104 76 0.6895391241921907 1105 1 -0.7941680581576627 1105 2 -0.001852792466586095 1105 3 -0.1434881573222171 1105 4 0.22209087981358522 1105 5 -0.2900820252107117 1105 6 0.03701178373186309 1105 7 -0.06311872286573816 1105 8 -0.061560942020456366 1105 9 -0.22758298512149366 1105 10-11 -0.21677495120168544 1105 12-13 -1.1378690233008726 1105 14-15 -0.5701461241718775 1105 16-17 -0.552071697053421 1105 18-19 -0.6152676800529093 1105 20-21 -0.4657615170468148 1105 22-23 -0.6796134781645478 1105 24-25 -0.8870976754844193 1105 26-27 -0.6006608344042164 1105 28-29 -0.8269070799912157 1105 30-31 -0.6595480235086981 1105 32-33 -0.15339892184449866 1105 34-35 -0.5622339985195595 1105 36-37 -0.6063597286699505 1105 38-39 -0.7536299387361609 1105 40-41 -0.6153726029984838 1105 42-43 -0.4650868866472493 1105 44-45 -0.29866348239372087 1105 46-47 -0.7129199343982293 1105 48-49 -0.5685030927090509 1105 50-51 -0.46676705867160706 1105 52-53 -0.6905329363055159 1105 54-55 -1.0090387234924378 1105 56-57 -0.7798222718431376 1105 58-59 -0.3804671998940634 1105 60-61 -0.4323196929597657 1105 62-63 -0.3859879875034693 1105 64-65 -0.33705374738826066 1105 66-67 -0.7049568026843644 1105 68-69 -0.42131613955844927 1105 70-71 -0.8156844533680001 1105 72-73 -0.5815916144895539 1105 74-75 -0.9047896457768303 1105 76 -1.1392462803914043 1106 1 0.1126284957023529 1106 2 0.3015088833034447 1106 3 -0.011746505202165025 1106 4 0.34992465423782093 1106 5 0.1517671780101928 1106 6 -0.025773282994869362 1106 7 -0.5955360887084069 1106 8 -0.468700983689466 1106 9 0.16038931061587647 1106 10-11 0.22746177383913846 1106 12-13 0.2034902874711335 1106 14-15 0.5272636398914159 1106 16-17 0.03686912168637235 1106 18-19 0.21002090601612622 1106 20-21 -0.05174608825014104 1106 22-23 -0.26991698624495797 1106 24-25 0.2357470792878047 1106 26-27 0.03081616317448521 1106 28-29 0.24140194641793045 1106 30-31 -0.18597969195850084 1106 32-33 -0.02107165116484566 1106 34-35 0.27707001634614414 1106 36-37 0.5703168860042993 1106 38-39 0.042293255137373365 1106 40-41 0.5242411139900867 1106 42-43 7.726775174035083E-4 1106 44-45 0.21365703114871337 1106 46-47 0.33984204544297825 1106 48-49 0.2568924813660658 1106 50-51 0.04651071597787393 1106 52-53 -0.05915729578521933 1106 54-55 0.03247825381484404 1106 56-57 -0.09719937640984 1106 58-59 0.4066717155854107 1106 60-61 0.003200325059268039 1106 62-63 -0.13398055465251701 1106 64-65 -0.23939622162667717 1106 66-67 -0.02630372537144865 1106 68-69 0.1883972452042073 1106 70-71 -0.13077539317316678 1106 72-73 0.048483277114712564 1106 74-75 -0.0025485285968045446 1106 76 0.10482151621876667 1107 1 -0.9117087247484932 1107 2 -0.9476382548713183 1107 3 -1.0299743363689693 1107 4 -1.027107163639947 1107 5 -0.24478569942297312 1107 6 -1.1842940972828089 1107 7 -1.0971080301117837 1107 8 -1.5826165670915486 1107 9 -0.5081540236836659 1107 10-11 -1.3196017058371083 1107 12-13 -0.993994606283465 1107 14-15 -1.2399106560795161 1107 16-17 -0.7663817869180534 1107 18-19 -0.5936034780281148 1107 20-21 -0.41187117465118206 1107 22-23 -0.5062600485205948 1107 24-25 -0.5196878245004513 1107 26-27 -0.8320793305934941 1107 28-29 -0.43922080994230583 1107 30-31 -0.5336351580781482 1107 32-33 -0.559723572054942 1107 34-35 -0.6165452259112101 1107 36-37 -0.6916287538093044 1107 38-39 -0.4456217485416403 1107 40-41 -0.22271533988848802 1107 42-43 -0.4613557980338925 1107 44-45 -0.6817029545811835 1107 46-47 -0.684595511693189 1107 48-49 -0.6164157306342091 1107 50-51 -0.7050277798637339 1107 52-53 -0.5730093326199324 1107 54-55 -0.3009907198523507 1107 56-57 -0.5701419169347091 1107 58-59 -0.6054188549958823 1107 60-61 -1.1553204892286857 1107 62-63 -0.6345547034629746 1107 64-65 -0.4714582519952444 1107 66-67 -0.24476456061860574 1107 68-69 -0.4296655878578477 1107 70-71 -0.8481380991420835 1107 72-73 -0.6048256037967974 1107 74-75 -0.8794967325701393 1107 76 -0.606587879754386 1108 1 0.03252380868784499 1108 2 0.05851789403505947 1108 3 -0.43133110330441227 1108 4 0.5499533356116579 1108 5 0.19976067689878718 1108 6 0.7410112959864676 1108 7 0.5678043352979003 1108 8 0.677848886049297 1108 9 0.29564588940600345 1108 10-11 0.17461634254297564 1108 12-13 -0.01360978662241763 1108 14-15 0.024610612811414967 1108 16-17 0.28928911260393875 1108 18-19 0.4875490496142021 1108 20-21 0.2638446285451863 1108 22-23 0.15646748757133366 1108 24-25 0.30798828964178426 1108 26-27 0.35841122997818076 1108 28-29 0.34873003743349074 1108 30-31 0.226093971369977 1108 32-33 0.13909589586066318 1108 34-35 0.36299463213524064 1108 36-37 -0.041044523207204975 1108 38-39 0.3388526853340821 1108 40-41 0.26328243906955606 1108 42-43 0.31680556549273575 1108 44-45 0.43032688463469526 1108 46-47 0.3056753588586858 1108 48-49 0.9041892618818466 1108 50-51 0.3594065786896863 1108 52-53 0.15374215209833153 1108 54-55 0.10850182034367606 1108 56-57 0.42012895361717284 1108 58-59 0.42601371706727775 1108 60-61 0.04156763785354656 1108 62-63 0.3714928075215269 1108 64-65 0.5674944784378582 1108 66-67 0.23828792356853512 1108 68-69 0.20374178020743017 1108 70-71 0.7573493230007387 1108 72-73 0.02117024382040711 1108 74-75 0.7143543610516048 1108 76 0.6875553291684078 1109 1 -0.39895228703132446 1109 2 -0.3222158168685567 1109 3 -0.40324293554075297 1109 4 -0.2904466847975442 1109 5 -0.7410016068911993 1109 6 -0.9921383545059044 1109 7 -0.2018012568241474 1109 8 -0.7488310466003298 1109 9 -0.5160791986086934 1109 10-11 -0.8546581102352206 1109 12-13 -0.6903152307777347 1109 14-15 -0.842997428415174 1109 16-17 -0.16999452431631568 1109 18-19 -0.353361200908779 1109 20-21 -0.1197463709955997 1109 22-23 -0.6318252824679575 1109 24-25 -0.7793503500847265 1109 26-27 -0.6431959948802373 1109 28-29 -0.529503545912597 1109 30-31 0.17758384330221588 1109 32-33 -0.3961865165073206 1109 34-35 -0.7674329407001181 1109 36-37 -0.6792531052777164 1109 38-39 -0.27339793969780857 1109 40-41 -0.565318509958459 1109 42-43 0.013690610648097845 1109 44-45 -0.301151762235051 1109 46-47 -0.4756017471161016 1109 48-49 -0.4483925024938813 1109 50-51 0.007845274541523395 1109 52-53 -0.46687624679956485 1109 54-55 -0.5060575959530667 1109 56-57 -0.40724744313776995 1109 58-59 -0.6511968549006824 1109 60-61 -0.10678176772837134 1109 62-63 -0.5627640423177453 1109 64-65 -0.10634092132743689 1109 66-67 -0.3453643035859102 1109 68-69 -0.12303509616381092 1109 70-71 -0.041186316572712656 1109 72-73 -0.43205441049868654 1109 74-75 -0.5200198417138324 1109 76 -0.6611359305897437 1110 1 -0.35295884996135385 1110 2 -0.6078355903416188 1110 3 -0.3466376640259874 1110 4 -0.18705407338651625 1110 5 -0.4709952556685906 1110 6 -0.016934586113819705 1110 7 -0.13715110333626512 1110 8 -0.2575639776895642 1110 9 -0.21828624846579459 1110 10-11 0.04360324257518755 1110 12-13 -0.249328674198928 1110 14-15 -0.0484727115738508 1110 16-17 -0.08274460370659398 1110 18-19 -0.30222062718166853 1110 20-21 0.08261945942675197 1110 22-23 -0.13459071064620787 1110 24-25 -0.13856491555524997 1110 26-27 -0.7063906598532412 1110 28-29 -0.03502117055534626 1110 30-31 -0.3599148335264104 1110 32-33 -0.09644850443999076 1110 34-35 0.005537314407334293 1110 36-37 0.3290564109708214 1110 38-39 -0.0057865286678548955 1110 40-41 -0.2314426763604871 1110 42-43 -0.18526143762119318 1110 44-45 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2116 24-25 -0.040306209087589195 2116 26-27 -0.07579364492786311 2116 28-29 0.23798877969340992 2116 30-31 -0.19449194795426905 2116 32-33 0.2860141821271682 2116 34-35 -0.20093034728421344 2116 36-37 -0.1734311512182316 2116 38-39 -0.03874672767283016 2116 40-41 -0.1064426763604871 2116 42-43 -0.24123158687491753 2116 44-45 -0.31740030674653497 2116 46-47 0.1130771985809389 2116 48-49 -0.09150671864716031 2116 50-51 0.12470777057200877 2116 52-53 0.11283159696035483 2116 54-55 0.035172424159135574 2116 56-57 0.023550693836931202 2116 58-59 0.08030520924666718 2116 60-61 0.11029772840797136 2116 62-63 -0.2038141111226608 2116 64-65 -0.449095234866121 2116 66-67 -0.1293433445512946 2116 68-69 -0.10890362585577407 2116 70-71 -0.1239903813551777 2116 72-73 0.2672151989011269 2116 74-75 0.12007754369952295 2116 76 0.39835385452722605 2117 1 1.5248347218620673 2117 2 0.61097116950992 2117 3 1.3703132954622887 2117 4 1.1774132724241149 2117 5 1.8548556485405214 2117 6 1.778828661141965 2117 7 1.6823032123867065 2117 8 2.2701940575311568 2117 9 1.5901912055340475 2117 10-11 2.0320637179759657 2117 12-13 1.447307194078732 2117 14-15 1.7385347116276009 2117 16-17 1.5883641382976705 2117 18-19 1.9679266522939685 2117 20-21 1.9164463566864853 2117 22-23 1.9237584167327668 2117 24-25 1.5983655116736841 2117 26-27 1.6313452637035795 2117 28-29 1.5589237871955888 2117 30-31 1.868960630423679 2117 32-33 1.7231143953467836 2117 34-35 1.9423216467140492 2117 36-37 1.9712028217739501 2117 38-39 1.7439666893689463 2117 40-41 1.7563070669862668 2117 42-43 1.6595028362475546 2117 44-45 1.792960587196447 2117 46-47 2.005401292397572 2117 48-49 2.076936778154547 2117 50-51 1.9612393981484075 2117 52-53 1.7691926488437986 2117 54-55 1.651771966131811 2117 56-57 1.572235839537008 2117 58-59 1.8775096631675439 2117 60-61 1.8554213168151392 2117 62-63 1.981252618720184 2117 64-65 2.018448001333823 2117 66-67 1.9228164911906305 2117 68-69 1.7731148531776242 2117 70-71 2.0981528706388204 2117 72-73 0.45705962755152285 2117 74-75 1.4568460819605917 2117 76 1.771488182885431 2118 1 0.39322096524831096 2118 2 0.7167912753300243 2118 3 0.5607894859384785 2118 4 0.6886566586675009 2118 5 0.4057815744664488 2118 6 1.3753895077028062 2118 7 0.8998958049793018 2118 8 0.7338183961554918 2118 9 1.3190271843700287 2118 10-11 0.7327912312034783 2118 12-13 0.9721087813803209 2118 14-15 0.5427675158604046 2118 16-17 0.8383641382976705 2118 18-19 1.0099240067913229 2118 20-21 0.8694886847288075 2118 22-23 1.3556367236110773 2118 24-25 0.6535903793985511 2118 26-27 1.518911401269719 2118 28-29 0.9749290782008799 2118 30-31 0.9192251806882368 2118 32-33 1.014781062013455 2118 34-35 0.9952316996240995 2118 36-37 0.8075123455834756 2118 38-39 1.0085169539192052 2118 40-41 0.9213202944994947 2118 42-43 1.164463153707878 2118 44-45 0.6914394231752823 2118 46-47 1.0292108162070974 2118 48-49 0.9936034448212112 2118 50-51 0.9582632076722177 2118 52-53 1.1560974107485578 2118 54-55 0.8862296380894819 2118 56-57 0.914497744298906 2118 58-59 0.723739821897702 2118 60-61 0.6126964490902722 2118 62-63 1.0599563224238935 2118 64-65 0.8001940330798618 2118 66-67 1.2217582901324278 2118 68-69 1.04097199603477 2118 70-71 1.3415391140250605 2118 72-73 1.2890702095621123 2118 74-75 1.24454449465901 2118 76 1.1226786590759126 2119 1 -0.06337020780627611 2119 2 0.08874249484221863 2119 3 -0.34135917840530894 2119 4 0.9480933601773707 2119 5 0.43089771848503133 2119 6 -0.037502478359911606 2119 7 0.280122285815537 2119 8 0.22496241473853473 2119 9 0.7275637697358803 2119 10-11 0.96137717777723 2119 12-13 0.4676807906718423 2119 14-15 0.17531687706829757 2119 16-17 0.6232073438725863 2119 18-19 0.3863032170119993 2119 20-21 0.1901913560412396 2119 22-23 0.3257296388259405 2119 24-25 0.1719556523369974 2119 26-27 0.1993701701431192 2119 28-29 -0.05149368602676674 2119 30-31 0.04698360112958255 2119 32-33 0.05107606782065233 2119 34-35 -0.09739315519587421 2119 36-37 -0.03474955917842948 2119 38-39 0.3611592303419755 2119 40-41 0.3011838252776613 2119 42-43 0.3282988099215771 2119 44-45 0.26047252422058165 2119 46-47 0.4786881681234689 2119 48-49 0.43436999534386445 2119 50-51 -0.20920194912220325 2119 52-53 -0.01144033605747552 2119 54-55 0.12047760556915676 2119 56-57 -0.07149238345950693 2119 58-59 -0.3161120944786049 2119 60-61 0.5859833248161692 2119 62-63 0.4819946499500247 2119 64-65 -0.16960852208855215 2119 66-67 0.2310498116190729 2119 68-69 0.019630532620134034 2119 70-71 0.26241600136535936 2119 72-73 -0.2604361783589155 2119 74-75 0.1574364807217279 2119 76 0.7267727357309646 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 26.0 13 273.0 14 1229.0 15 2102.0 16 2523.0 17 2832.0 18 2881.0 19 2181.0 20 1644.0 21 1152.0 22 829.0 23 597.0 24 490.0 25 439.0 26 470.0 27 453.0 28 546.0 29 684.0 30 932.0 31 1166.0 32 1571.0 33 2129.0 34 3301.0 35 5434.0 36 11558.0 37 48393.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.097833799407326 21.76739429859343 16.232939605158812 27.901832296840436 2 17.668725739805502 23.18752869485371 33.31629032931257 25.827455236028214 3 16.887182269710756 25.97353812763471 28.505989398555865 28.633290204098667 4 13.594056513210068 20.16048249092199 31.675988146416795 34.569472849451145 5 13.813180850619808 33.61262990942861 33.73158312116533 18.84260611878626 6 26.03614508118035 33.38411452898702 20.91907007805 19.66067031178263 7 25.29007888476147 29.377269502066028 21.56600859802162 23.766643015150883 8 22.46024458449852 31.12609040444092 22.825451813514753 23.58821319754581 9 21.55348720731249 18.774781919111817 22.43728870153178 37.234442172043906 10-11 24.199674443841563 24.446971910346843 26.18588004507701 25.167473600734585 12-13 22.90005843315664 22.27868441921616 25.754413790224966 29.066843357402227 14-15 22.581284694686758 23.320046746525314 23.779164405860012 30.319504152927916 16-17 23.476564130389416 24.871134020618555 24.562273049793397 27.090028799198627 18-19 22.498852205851662 23.840206185567013 25.695980633582373 27.96496097499896 20-21 22.036604198839687 24.70470386910973 25.827455236028214 27.43123669602237 22-23 24.01081013397888 24.223673776034058 24.55549062982595 27.21002546016111 24-25 22.8056262782253 25.348512041404064 24.08489502900789 27.760966651362747 26-27 22.173817772027213 25.386597938144327 25.36207688133895 27.077507408489502 28-29 22.92782945776878 24.672485665541473 26.444793422061764 25.954891454627983 30-31 22.735715180099337 24.11776367961935 26.56100004173797 26.585521098543346 32-33 21.737134271046372 25.208168120539256 26.196836261947492 26.857861346466883 34-35 22.275032346926 24.862264702199592 26.47074168370967 26.39196126716474 36-37 22.521286364205515 23.849597228598856 26.49056721899912 27.138549188196503 38-39 21.698004925080348 23.863162068533743 28.363558579239534 26.07527442714638 40-41 21.544096164280646 23.43586961058475 28.213823615342875 26.806210609791727 42-43 20.340477482365706 23.538127634709294 28.239909845986894 27.881485036938102 44-45 19.81979631871113 23.32891606494428 29.45448474477232 27.39680287157227 46-47 20.138570057181017 24.138110939521685 29.33970532993865 26.383613673358653 48-49 19.993008890187404 24.526795776117535 28.778851371092284 26.70134396260278 50-51 20.15526524479319 25.18677741141116 27.539233690888604 27.11872365290705 52-53 19.188509537125924 25.233732626570394 27.692620727075422 27.885137109228264 54-55 19.282419967444383 24.672878667724028 27.70775074084895 28.336950623982638 56-57 19.615801995074918 24.70522559372261 27.602884093660002 28.07608831754247 58-59 19.623627864268126 24.772528068784172 27.18185233106557 28.42199173588213 60-61 19.452502191243372 24.30610626486915 27.320109353478863 28.921282190408615 62-63 19.445198046663048 24.780875662590258 26.799949914437164 28.97397637630953 64-65 20.290391919529196 24.444363287282442 25.86658458199424 29.398660211194123 66-67 19.765015234358696 25.40485829959514 25.056867982803954 29.773258483242206 68-69 20.235194682555 25.50411903730872 24.893698055522222 29.366988224614055 70-71 20.998893943820693 25.303122000083476 24.61183688801703 29.086147168078803 72-73 21.092804374139153 25.372511373596563 24.590446178888936 28.94423807337535 74-75 20.938373888726574 24.998434826161358 25.43407487791644 28.629116407195625 76 20.43490963729705 24.20906548687341 24.270628991193288 31.085395884636252 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.0 12 1.0 13 1.0 14 4.5 15 7.0 16 11.5 17 17.0 18 16.5 19 20.0 20 30.5 21 37.0 22 54.5 23 89.5 24 115.5 25 124.0 26 202.5 27 336.0 28 452.0 29 513.0 30 631.0 31 875.5 32 1174.0 33 1346.0 34 1531.5 35 1945.5 36 2350.0 37 2526.0 38 2913.5 39 3675.0 40 4396.0 41 4957.5 42 5172.0 43 5245.5 44 5580.0 45 5750.5 46 5660.0 47 5684.0 48 5458.0 49 4851.5 50 4495.0 51 4239.5 52 3628.0 53 2969.5 54 2667.0 55 2626.5 56 2320.0 57 1929.5 58 1805.0 59 1749.5 60 1726.0 61 1788.0 62 1818.0 63 1872.5 64 1949.0 65 1976.5 66 1941.0 67 1900.0 68 1747.0 69 1488.5 70 1226.5 71 1070.0 72 913.0 73 630.0 74 383.5 75 263.0 76 178.5 77 70.0 78 31.0 79 16.0 80 11.0 81 6.5 82 5.0 83 3.0 84 4.0 85 4.0 86 3.5 87 4.0 88 3.5 89 2.5 90 3.0 91 4.5 92 5.0 93 4.5 94 3.0 95 2.0 96 2.0 97 3.0 98 4.0 99 14.5 100 25.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 5.217246128803372E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 5.217246128803372E-4 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 95836.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.089778371384455 #Duplication Level Percentage of deduplicated Percentage of total 1 75.62600348443958 46.19975791977962 2 12.038397157790454 14.708460286322467 3 4.825265603115499 8.843232188321716 4 2.471560823967479 6.039484118702784 5 1.479178765415229 4.51813514754372 6 0.9735934137259591 3.5685963521015065 7 0.6780992723670276 2.899745398388914 8 0.4338468896252519 2.1202888267456905 9 0.3706487206640932 2.037856337910597 >10 1.1034058688894204 9.06444342418298 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.008347593806085397 0.0 2 0.0 0.0 0.0 0.01147794148336742 0.0 3 0.0 0.0 0.0 0.012521390709128093 0.0 4 0.0 0.0 0.0 0.02817312909553821 0.0 5 0.0 0.0 0.0 0.03026002754705956 0.0 6 0.0 0.0 0.0 0.03026002754705956 0.0 7 0.0 0.0 0.0 0.031303476772820235 0.0 8 0.0 0.0 0.0 0.03234692599858091 0.0 9 0.0 0.0 0.0 0.03547727367586293 0.0 10 0.0 0.0 0.0 0.037564172127384284 0.0 11 0.0 0.0 0.0 0.03965107057890563 0.0 12 0.0 0.0 0.0 0.03965107057890563 0.0 13 0.0 0.0 0.0 0.040694519804666304 0.0 14 0.0 0.0 0.0 0.04695521515923035 0.0 15 0.0 0.0 0.0 0.04695521515923035 0.0 16 0.0 0.0 0.0 0.04695521515923035 0.0 17 0.0 0.0 0.0 0.0490421136107517 0.0 18 0.0 0.0 0.0 0.055302808965315746 0.0 19 0.0 0.0 0.0 0.06365040277140115 0.0 20 0.0 0.0 0.0 0.06991109812596519 0.0 21 0.0 0.0 0.0 0.07304144580324722 0.0 22 0.0 0.0 0.0 0.07617179348052924 0.0 23 0.0 0.0 0.0 0.07825869193205058 0.0 24 0.0 0.0 0.0 0.08556283651237531 0.0 25 0.0 0.0 0.0 0.09078008264117868 0.0 26 0.0 0.0 0.0 0.09912767644726407 0.0 27 0.0 0.0 0.0 0.1752994699277933 0.0 28 0.0 0.0 0.0 0.25981885721440795 0.0 29 0.0 0.0 0.0 0.3276430568888518 0.0 30 0.0 0.0 0.0 0.40381485036938103 0.0 31 0.0 0.0 0.0 0.5300722066864226 0.0 32 0.0 0.0 0.0 0.666764055261071 0.0 33 0.0 0.0 0.0 0.8243248883509329 0.0 34 0.0 0.0 0.0 0.9881464167953588 0.0 35 0.0 0.0 0.0 1.211444551108143 0.0 36 0.0 0.0 0.0 1.5630869401894905 0.0 37 0.0 0.0 0.0 2.0618556701030926 0.0 38 0.0 0.0 0.0 2.596101673692558 0.0 39 0.0 0.0 0.0 3.1011310989607246 0.0 40 0.0 0.0 0.0 3.638507450227472 0.0 41 0.0 0.0 0.0 4.2614466380065945 0.0 42 0.0 0.0 0.0 4.88855962268876 0.0 43 0.0010434492257606746 0.0 0.0 5.644016862139488 0.0 44 0.0010434492257606746 0.0 0.0 6.29617262823991 0.0 45 0.0010434492257606746 0.0 0.0 6.896155933052298 0.0 46 0.0010434492257606746 0.0 0.0 7.488835093284361 0.0 47 0.0010434492257606746 0.0 0.0 8.10655703493468 0.0 48 0.0010434492257606746 0.0 0.0 8.67941065987729 0.0 49 0.0010434492257606746 0.0 0.0 9.253307734045661 0.0 50 0.0010434492257606746 0.0 0.0 9.785466839183606 0.0 51 0.0010434492257606746 0.0 0.0 10.454317792896198 0.0 52 0.0010434492257606746 0.0 0.0 11.165950164864977 0.0 53 0.0010434492257606746 0.0 0.0 11.883843232188323 0.0 54 0.0010434492257606746 0.0 0.0 12.465044450937018 0.0 55 0.0010434492257606746 0.0 0.0 13.030593931299302 0.0 56 0.0010434492257606746 0.0 0.0 13.534579907341708 0.0 57 0.0010434492257606746 0.0 0.0 14.115781126090404 0.0 58 0.0010434492257606746 0.0 0.0 14.611419508326724 0.0 59 0.0010434492257606746 0.0 0.0 15.101840644434242 0.0 60 0.0010434492257606746 0.0 0.0 15.554697608414374 0.0 61 0.0010434492257606746 0.0 0.0 16.028423556909722 0.0 62 0.0010434492257606746 0.0 0.0 16.590842689594723 0.0 63 0.0010434492257606746 0.0 0.0 17.115697650152345 0.0 64 0.0010434492257606746 0.0 0.0 17.764723068575485 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTACC 20 0.0069326977 52.5 24 TTCAGCA 35 0.0012500165 40.0 18 CCTATGA 40 0.0024084174 35.0 14 ATCGAAG 40 0.0024084174 35.0 8 TAACATA 55 2.9731626E-4 31.81818 1 CTTATAA 45 0.004288964 31.111113 1 TGAATGG 45 0.004288964 31.111113 5 TTTGTGG 50 0.0071779373 28.000002 52 TATCGAA 50 0.0071779373 28.000002 7 AACATCT 65 7.905914E-4 26.923077 7 ATATAGG 85 1.2968236E-4 24.705883 1 CTGGCCC 75 0.001820851 23.333334 46 GCAGTCC 170 3.6507117E-9 22.64706 70 CGCCATC 80 0.002649514 21.875 18 AAACTCT 85 0.0037658298 20.588236 36 CCCAGCT 90 0.0052423305 19.444445 50 CTCCAGC 100 0.0096275965 17.500002 59 ATAGTAG 140 1.8432563E-4 17.5 3 AGCAGTC 240 1.9059735E-7 16.041666 69 TACACAA 155 0.007309767 13.548387 5 >>END_MODULE