FastQCFastQC Report
Wed 25 May 2016
SRR1780344_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780344_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95836
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT73737.693351141533452No Hit
CACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTTCTG26052.7181852331065572Illumina PCR Primer Index 8 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG18141.8928168955298634No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC15941.6632580658625151No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC5640.5885053633290205No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT4540.47372594849534627RNA PCR Primer, Index 15 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT2220.23164572811886974No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC1640.17112567302475062No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGT1540.16069118076714387No Hit
CTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1520.1586042823156225No Hit
CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1300.13564839934888767No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT1290.134604950123127No Hit
TATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCT1040.10851871947911015No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATTC200.006932697752.515
GGATCGG200.006932697752.510
ATACAGG200.006932697752.51
TGACTCG200.006932697752.528
TCTTTAT506.121809E-849.0000041
CTTTATA506.121809E-849.0000042
AAGCTCG451.669543E-646.66666838
GTTAACG305.8523094E-446.66666463
GCGACAA350.001250016540.016
TATAAAC350.001250016540.02
TTTATAC801.3969839E-939.3753
TCTTAAT556.635175E-638.1818161
TTAATAC501.6988289E-435.0000042
ATGGCCA400.002408417435.05
AAAAGAC400.002408417435.069
ATAAACT400.002408417435.03
AATCTCG15400.033.40909236
CTTGAAA16500.033.3030357
TCTGCTT16500.033.3030353
TCGTATG16950.033.24483540
Produced by FastQC (version 0.11.5)