FastQCFastQC Report
Wed 25 May 2016
SRR1780343_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780343_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453534
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA353.242538E-431.382421
TACACAG300.00578265329.290265
CAGTGTA450.001388186424.47060434
CTTATGC551.6074178E-423.9647581
CAAAATA956.7839574E-720.86210439
GTAGCAA708.0604566E-418.87523735
GTCTATG708.138663E-418.84815629
CGATTTC600.007326779418.36716515
TGCAATG600.007383068618.34281512
TACTAGA600.00742551818.32459629
GCTATAT600.007468166818.3064141
CAAGATA600.007468166818.3064144
AGCAAAA750.001280916417.61688637
CTAAAAG902.1990853E-417.12374513
TGTAAGG902.2364932E-417.0859852
AAGAGCA800.00196143716.53044917
GCGTGTA800.001971614516.51765834
ACGAGAG800.001978911516.50853324
TGTTGTC800.002005369216.4757737
TAAGAAC800.002005369216.4757734
Produced by FastQC (version 0.11.5)