FastQCFastQC Report
Wed 25 May 2016
SRR1780343_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780343_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453534
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT227155.008444791349712No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC67761.4940445479280495No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG57191.2609859459268764No Hit
CACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTTCTG34630.7635590716462272Illumina PCR Primer Index 1 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC11470.252902759219816No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTT6000.1322943814576195Illumina PCR Primer Index 1 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTTGT250.002352201635.1970919
TATTAAG250.002352201635.1970915
AGCTAGT250.002352201635.197096
ATGCCGT31050.034.71915444
TCTTCTG4500.034.22316444
CGTCTTC4650.032.6460642
TGATTCG353.2141968E-431.42943843
CCGTCTT5000.030.36083841
GTCTTCT5000.030.36083843
TCTTTAT1600.030.3109931
CCTTATA300.005685026329.3924791
GCCTTAC300.005685026329.3924791
TGCTAGA454.0085244E-529.3924791
TGTTACG300.00574359829.3309116
GCTAGTC300.00574359829.330917
TATGCCG37450.028.7858443
CTTTATA1750.027.6548542
CTATAAC407.02871E-427.4977254
AAAAAGC407.02871E-427.49772515
GCTAGAA450.001397541824.4424212