Basic Statistics
Measure | Value |
---|---|
Filename | SRR1780343_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 453534 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 59 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT | 22715 | 5.008444791349712 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC | 6776 | 1.4940445479280495 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG | 5719 | 1.2609859459268764 | No Hit |
CACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTTCTG | 3463 | 0.7635590716462272 | Illumina PCR Primer Index 1 (96% over 25bp) |
TCTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC | 1147 | 0.252902759219816 | No Hit |
ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTT | 600 | 0.1322943814576195 | Illumina PCR Primer Index 1 (95% over 22bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AAGTTGT | 25 | 0.0023522016 | 35.19709 | 19 |
TATTAAG | 25 | 0.0023522016 | 35.19709 | 15 |
AGCTAGT | 25 | 0.0023522016 | 35.19709 | 6 |
ATGCCGT | 3105 | 0.0 | 34.719154 | 44 |
TCTTCTG | 450 | 0.0 | 34.223164 | 44 |
CGTCTTC | 465 | 0.0 | 32.64606 | 42 |
TGATTCG | 35 | 3.2141968E-4 | 31.429438 | 43 |
CCGTCTT | 500 | 0.0 | 30.360838 | 41 |
GTCTTCT | 500 | 0.0 | 30.360838 | 43 |
TCTTTAT | 160 | 0.0 | 30.310993 | 1 |
CCTTATA | 30 | 0.0056850263 | 29.392479 | 1 |
GCCTTAC | 30 | 0.0056850263 | 29.392479 | 1 |
TGCTAGA | 45 | 4.0085244E-5 | 29.392479 | 1 |
TGTTACG | 30 | 0.005743598 | 29.33091 | 16 |
GCTAGTC | 30 | 0.005743598 | 29.33091 | 7 |
TATGCCG | 3745 | 0.0 | 28.78584 | 43 |
CTTTATA | 175 | 0.0 | 27.654854 | 2 |
CTATAAC | 40 | 7.02871E-4 | 27.497725 | 4 |
AAAAAGC | 40 | 7.02871E-4 | 27.497725 | 15 |
GCTAGAA | 45 | 0.0013975418 | 24.442421 | 2 |