FastQCFastQC Report
Wed 25 May 2016
SRR1780342_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780342_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88869
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTGTA200.00693104852.50000433
ATATCTC404.5604946E-543.75000440
TGCCGCT404.5604946E-543.75000413
CCACTGC350.001249526640.057
GCTCCCG400.002407478635.00000418
GCTTCTG501.6978379E-435.060
CGTGAGC450.00428730431.11110926
GGCCCGT450.00428730431.1111096
GGTTAGT450.00428730431.1111091
ATCTGCC450.00428730431.11110910
TATATCT755.5310935E-528.039
TTCTACA500.007175177327.9999982
GTTAGTA500.007175177327.9999982
ACTCTGC657.9013663E-426.92307715
TTATCCA657.9013663E-426.9230772
CTGACTC700.001217666725.09
CTTATCC1052.0133135E-523.3333341
CGCTTCC800.002648012721.87500216
TCTACAC850.003763707620.5882363
ACATCTT850.003763707620.5882368
Produced by FastQC (version 0.11.5)