FastQCFastQC Report
Wed 25 May 2016
SRR1780342_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780342_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88869
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT64157.2184901371681915No Hit
CACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTTCTG22352.514937717314249RNA PCR Primer, Index 47 (96% over 25bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC17761.998447152550383No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG16841.894923989242593No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC4310.48498351506149506No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT2950.33194927364997917Illumina PCR Primer Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT2320.2610584118196447No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT1830.20592107484049557No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG1030.11590093283372153No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC1000.11252517750846751No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG940.10577366685795948No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTTGT150.002218135970.016
TCTTTAT352.9445982E-760.01
CTTTATA451.6681624E-646.6666642
ACTCGTA404.5604946E-543.75000438
AACTCGT404.5604946E-543.75000437
TAAGAAC459.133527E-538.88888533
CTACTCT400.002407478635.00000428
TCTGCTT15900.034.33962253
TTCTGCT15950.034.23197652
CCGTCTT15850.034.22712747
TCTTCTG15900.034.11949550
GTCTTCT15900.034.11949549
GTATGCC16100.033.91304442
CTTGAAA16000.033.9062557
GCTTGAA16250.033.81538456
TATGCCG16150.033.80805243
TCGTATG16150.033.80805240
TGCCGTC16050.033.8006245
CTGCTTG16200.033.70370554
CGTATGC16200.033.70370541