##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780342_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 88869 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.59659723863214 34.0 34.0 34.0 34.0 34.0 2 33.63793898884875 34.0 34.0 34.0 34.0 34.0 3 33.671280198944515 34.0 34.0 34.0 34.0 34.0 4 33.688203985641785 34.0 34.0 34.0 34.0 34.0 5 33.659712610696644 34.0 34.0 34.0 34.0 34.0 6 37.34993079701583 38.0 38.0 38.0 36.0 38.0 7 37.369273875029535 38.0 38.0 38.0 37.0 38.0 8 37.233759803756094 38.0 38.0 38.0 36.0 38.0 9 37.28814322204593 38.0 38.0 38.0 36.0 38.0 10-11 37.21335898907381 38.0 38.0 38.0 36.0 38.0 12-13 37.174284621183986 38.0 38.0 38.0 36.0 38.0 14-15 37.09044211142243 38.0 38.0 38.0 36.0 38.0 16-17 37.12535867400331 38.0 38.0 38.0 36.0 38.0 18-19 37.05738221427045 38.0 38.0 38.0 35.5 38.0 20-21 37.09345215992079 38.0 38.0 38.0 36.0 38.0 22-23 37.08459080219199 38.0 38.0 38.0 36.0 38.0 24-25 37.05786044627486 38.0 38.0 38.0 35.5 38.0 26-27 36.9425165130698 38.0 38.0 38.0 35.0 38.0 28-29 36.93034691512226 38.0 38.0 38.0 35.0 38.0 30-31 36.89670188704723 38.0 37.5 38.0 35.0 38.0 32-33 36.750227863484454 38.0 37.0 38.0 34.0 38.0 34-35 36.81610010239791 38.0 37.0 38.0 35.0 38.0 36-37 36.81816493940519 38.0 37.0 38.0 34.5 38.0 38-39 35.97971171049522 38.0 37.0 38.0 33.0 38.0 40-41 36.37619417344631 38.0 37.0 38.0 34.0 38.0 42-43 36.72074064071836 38.0 37.0 38.0 34.0 38.0 44-45 36.82393748101138 38.0 37.0 38.0 34.5 38.0 46-47 36.74936141961764 38.0 37.0 38.0 34.5 38.0 48-49 36.769058951940494 38.0 37.0 38.0 34.5 38.0 50-51 36.582835409422856 38.0 37.0 38.0 34.0 38.0 52-53 36.55859748618754 38.0 37.0 38.0 34.0 38.0 54-55 36.54332781959964 38.0 37.0 38.0 34.0 38.0 56-57 36.59923032778584 38.0 37.0 38.0 34.0 38.0 58-59 36.43358764023451 38.0 37.0 38.0 34.0 38.0 60-61 36.41861053910813 38.0 37.0 38.0 34.0 38.0 62-63 36.4172658632369 38.0 37.0 38.0 34.0 38.0 64-65 36.26095151290101 38.0 37.0 38.0 33.5 38.0 66-67 35.804380605160404 38.0 37.0 38.0 31.5 38.0 68-69 35.68418121054586 38.0 37.0 38.0 31.0 38.0 70-71 34.88698533796937 38.0 36.0 38.0 26.5 38.0 72-73 33.609014391970206 38.0 34.5 38.0 20.0 38.0 74-75 33.24645264377905 38.0 34.0 38.0 15.0 38.0 76 32.525920174639076 37.0 31.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14133152324793485 1101 2 0.1733979600501172 1101 3 0.15378389086621524 1101 4 0.165920832223172 1101 5 -0.10148230714585793 1101 6 -0.20969100121052975 1101 7 0.15988330974291642 1101 8 -0.09849988110998709 1101 9 0.14802336343763756 1101 10-11 0.021479196132631273 1101 12-13 0.16738130304970866 1101 14-15 0.2614079509356628 1101 16-17 0.3731421225861169 1101 18-19 0.15196754215443065 1101 20-21 0.1502118107257786 1101 22-23 0.13591736834683843 1101 24-25 0.20392645312359292 1101 26-27 -0.09913662097870457 1101 28-29 -0.3180496874311203 1101 30-31 0.014879693021498497 1101 32-33 -0.13891827044307803 1101 34-35 0.11326375750008566 1101 36-37 0.009448558941187457 1101 38-39 0.25497285284224347 1101 40-41 0.04691256828860446 1101 42-43 0.1429474780234301 1101 44-45 0.18261327595230625 1101 46-47 0.2186967749232167 1101 48-49 0.2527492812444052 1101 50-51 0.2423484726646521 1101 52-53 0.29000529477780645 1101 54-55 -0.06970414587135565 1101 56-57 -0.06053755921778503 1101 58-59 7.903310081545101E-4 1101 60-61 -0.31279738679299385 1101 62-63 -0.05835441854268453 1101 64-65 -0.24559327606972658 1101 66-67 0.11520143548379735 1101 68-69 -0.29455708079653675 1101 70-71 -0.1279009637062174 1101 72-73 0.2407881777275236 1101 74-75 0.26178719107918624 1101 76 -0.4446109433900318 1102 1 0.22051952028582633 1102 2 0.23603680869302224 1102 3 0.1881217639464765 1102 4 0.23524051411892088 1102 5 0.040103062634237574 1102 6 0.23501101752800224 1102 7 0.21506860225072444 1102 8 0.2113619946748031 1102 9 0.14362850204480537 1102 10-11 0.21833035038743276 1102 12-13 0.30325174317978565 1102 14-15 0.20762321865194622 1102 16-17 0.259181595846691 1102 18-19 0.3653115004273957 1102 20-21 0.272905715874046 1102 22-23 0.23864926570348644 1102 24-25 0.3590666701299412 1102 26-27 0.5032974561003982 1102 28-29 0.41135241823872803 1102 30-31 0.41939207268600853 1102 32-33 0.6723683131558786 1102 34-35 0.5872326554040725 1102 36-37 0.5051583371053283 1102 38-39 0.4987749713908016 1102 40-41 0.659062025772748 1102 42-43 0.2577531704153273 1102 44-45 0.2935392555395424 1102 46-47 0.30632033009240445 1102 48-49 0.26714929412311506 1102 50-51 0.4209199012360827 1102 52-53 0.5697953717281266 1102 54-55 0.609503169238593 1102 56-57 0.35133983541823 1102 58-59 0.590997678314892 1102 60-61 0.557996907936392 1102 62-63 0.4814247115001393 1102 64-65 0.7249869100277024 1102 66-67 0.9233729799636521 1102 68-69 0.9299350148923082 1102 70-71 0.7356812507887796 1102 72-73 0.23001835255607261 1102 74-75 0.5052673417601454 1102 76 0.6849167297481884 1103 1 0.0914791498661458 1103 2 0.04973227756090637 1103 3 0.02875550113193981 1103 4 0.037826351187511875 1103 5 0.1691286399356784 1103 6 0.022877701946406148 1103 7 0.07653104992007087 1103 8 0.005974341011288686 1103 9 -0.0499098639073523 1103 10-11 0.1578634795757381 1103 12-13 0.2952486662111937 1103 14-15 0.1595455843674074 1103 16-17 0.0339415775032208 1103 18-19 0.19449536409401702 1103 20-21 0.4705047927834656 1103 22-23 0.5383134619194081 1103 24-25 0.2752414656150748 1103 26-27 0.14342526864201233 1103 28-29 0.047042295307917925 1103 30-31 0.10328532554477476 1103 32-33 0.15446124352467194 1103 34-35 -0.07410848869568554 1103 36-37 0.23900795026528954 1103 38-39 0.3373598616998379 1103 40-41 0.5665014849363459 1103 42-43 0.21727180234776 1103 44-45 0.40638116148489445 1103 46-47 0.07504471950645097 1103 48-49 0.3426311799498336 1103 50-51 0.2162866966028716 1103 52-53 0.40038458162767654 1103 54-55 0.6395849751824727 1103 56-57 -0.03158003026025824 1103 58-59 0.15630929535065263 1103 60-61 0.06546743264829047 1103 62-63 0.017275833558159093 1103 64-65 0.07390479342834766 1103 66-67 0.13552848522261485 1103 68-69 0.29549515631452294 1103 70-71 0.466239395140029 1103 72-73 0.9431502036189556 1103 74-75 0.6306833982106852 1103 76 0.4774876257673597 1104 1 0.08680304519004522 1104 2 0.19210321993184465 1104 3 0.06308983546627189 1104 4 0.144819058180218 1104 5 0.2498093206163574 1104 6 0.36923834830705715 1104 7 0.25568162907065073 1104 8 0.09054462558157184 1104 9 -0.019079033076522478 1104 10-11 0.0617530834653337 1104 12-13 0.23779835876088384 1104 14-15 0.19108426590609184 1104 16-17 0.20739360095524262 1104 18-19 0.27118634078499326 1104 20-21 0.25234377462244595 1104 22-23 0.3562099298158685 1104 24-25 0.18480102517464303 1104 26-27 0.30674573196247223 1104 28-29 0.2080318562974739 1104 30-31 0.3441047163641642 1104 32-33 0.033926422989857485 1104 34-35 0.06609600150879658 1104 36-37 0.09755935881669586 1104 38-39 0.21129093563091317 1104 40-41 0.06231873075359573 1104 42-43 0.12601626109221797 1104 44-45 -0.05467274956901491 1104 46-47 0.0546600491217788 1104 48-49 0.09409693141557796 1104 50-51 -0.19019120987503157 1104 52-53 -0.27298879174568924 1104 54-55 -0.09164625604876164 1104 56-57 -0.32720422588445075 1104 58-59 0.12573586477721932 1104 60-61 0.0868602540411132 1104 62-63 0.06833403461635612 1104 64-65 0.06234323186678381 1104 66-67 -0.5340982844041591 1104 68-69 -0.3786218178024541 1104 70-71 -0.2345614056607772 1104 72-73 -0.13722302675427045 1104 74-75 -0.4490749315476421 1104 76 -0.4493106010308665 1105 1 0.028085434298517953 1105 2 0.1174143017646685 1105 3 0.1442346166110582 1105 4 -0.027688627370942243 1105 5 0.1573777586195817 1105 6 0.14896313237966297 1105 7 0.10232194527618077 1105 8 0.17665207690641438 1105 9 0.22858602328418698 1105 10-11 0.3066952588422964 1105 12-13 0.28629052681826295 1105 14-15 0.19257013913109944 1105 16-17 -0.11966685871391292 1105 18-19 0.26070471291205877 1105 20-21 0.2203865209260698 1105 22-23 -0.014504460463733437 1105 24-25 0.09684172199794006 1105 26-27 0.3537813050850005 1105 28-29 0.2021615591228354 1105 30-31 0.11114468123021481 1105 32-33 0.0911362019387596 1105 34-35 0.22102939339870886 1105 36-37 0.049696673997488006 1105 38-39 0.15796053012658717 1105 40-41 0.38478249886953364 1105 42-43 0.08605871461293191 1105 44-45 -0.10604882920596026 1105 46-47 0.11026684168509604 1105 48-49 0.12959685821985545 1105 50-51 -0.13243978255838584 1105 52-53 0.5203503972456716 1105 54-55 -0.10130809179754863 1105 56-57 0.1608063142999967 1105 58-59 0.1390721259395633 1105 60-61 0.23878530161833567 1105 62-63 0.0827755512317907 1105 64-65 -0.32055092711433275 1105 66-67 0.15575678805961246 1105 68-69 0.6304105347081617 1105 70-71 0.34184029248005743 1105 72-73 0.11066893635508279 1105 74-75 0.2836508772651172 1105 76 0.8750489363721456 1106 1 0.1353673398217552 1106 2 0.07033875884154384 1106 3 -0.03724308172057533 1106 4 -0.09288381323051453 1106 5 0.22642188824016074 1106 6 0.15196826923922657 1106 7 0.018977509220462707 1106 8 0.24352007293904165 1106 9 0.29111655577075624 1106 10-11 0.07219836020274784 1106 12-13 0.2759797986277164 1106 14-15 -0.01576134374850824 1106 16-17 0.1899362559698119 1106 18-19 0.15079516309045005 1106 20-21 0.09674660441854144 1106 22-23 0.04075008795152968 1106 24-25 -0.01309743508337391 1106 26-27 -0.22096962384276964 1106 28-29 -0.020973553606808082 1106 30-31 -0.10264934106405121 1106 32-33 0.06904918620249845 1106 34-35 -0.11178581288987033 1106 36-37 0.1411090883214854 1106 38-39 -0.025870945351201158 1106 40-41 0.2804835247610882 1106 42-43 -0.058898428878649156 1106 44-45 -0.01986209621903612 1106 46-47 -0.11614727505632771 1106 48-49 0.13363084735399156 1106 50-51 -0.01915500513096191 1106 52-53 0.19388594816275884 1106 54-55 0.37963963658545197 1106 56-57 0.1735448377859612 1106 58-59 -0.039141372010135456 1106 60-61 0.4126613360219693 1106 62-63 0.24413511659721365 1106 64-65 0.6009824328655284 1106 66-67 -0.5311020297225113 1106 68-69 0.055252505959515474 1106 70-71 0.29694088314489164 1106 72-73 0.16970581219703718 1106 74-75 0.2133262336625137 1106 76 0.003914534216740151 1107 1 0.07989613828313225 1107 2 0.14625737408599804 1107 3 0.22180569418213736 1107 4 -0.027810714449550744 1107 5 -0.06639259558555466 1107 6 0.053765732834442304 1107 7 0.2695812429702613 1107 8 0.24535658039353336 1107 9 0.17788936389187882 1107 10-11 0.11346193517418612 1107 12-13 0.3106926816551976 1107 14-15 0.39506681988864756 1107 16-17 0.20961725317890512 1107 18-19 -0.04874787914922507 1107 20-21 0.3025511248298045 1107 22-23 0.11746404106998654 1107 24-25 0.06481675519037111 1107 26-27 0.2592553844721195 1107 28-29 0.45051719878282626 1107 30-31 0.4025131247725753 1107 32-33 0.5289786180420464 1107 34-35 0.3814899669027625 1107 36-37 0.29499250624984086 1107 38-39 0.3122633366033085 1107 40-41 0.28657870501356086 1107 42-43 0.3080053930813449 1107 44-45 0.5646823197860584 1107 46-47 0.21404085850259236 1107 48-49 0.20170453902318997 1107 50-51 0.25103573135191226 1107 52-53 0.4119675932106901 1107 54-55 0.25734559294308923 1107 56-57 0.12286012417990122 1107 58-59 0.007660646701999951 1107 60-61 -0.30904994186907686 1107 62-63 0.20839552467784728 1107 64-65 0.5854878050113541 1107 66-67 0.5061848558789848 1107 68-69 0.5870009478203144 1107 70-71 0.5550424839431187 1107 72-73 0.12268688315563736 1107 74-75 0.3584826260099163 1107 76 0.06822121650094459 1108 1 -0.02589536750836885 1108 2 -0.013036613779419781 1108 3 0.0485017780210768 1108 4 0.0228235933276153 1108 5 0.00884182250599963 1108 6 -0.0325001248599861 1108 7 0.1985387719277938 1108 8 0.16416518491642051 1108 9 0.12317342346165105 1108 10-11 0.06817787560441957 1108 12-13 -0.1583089730607412 1108 14-15 0.24796257278440237 1108 16-17 0.13184560397867529 1108 18-19 -0.12216210970630925 1108 20-21 0.1623966846753575 1108 22-23 -0.286231491196979 1108 24-25 0.031777593579782604 1108 26-27 -0.1075399823232388 1108 28-29 0.011962317370795006 1108 30-31 0.00929746012833732 1108 32-33 0.01276240182583166 1108 34-35 -0.0690891836763825 1108 36-37 0.05224567778873279 1108 38-39 -0.029600976689579284 1108 40-41 0.12524390082162284 1108 42-43 0.04804951883976116 1108 44-45 -0.06846715622056365 1108 46-47 0.17692006423894213 1108 48-49 0.25244916119638106 1108 50-51 -0.019896425294525955 1108 52-53 0.08196207749089268 1108 54-55 -0.0585396800850404 1108 56-57 0.1864012162924169 1108 58-59 -0.6137955033255764 1108 60-61 -0.0624972667449768 1108 62-63 -0.043948656237759565 1108 64-65 0.2880801933180308 1108 66-67 -0.1109690327034798 1108 68-69 -0.03765431969209487 1108 70-71 -0.1287412998406694 1108 72-73 -0.39198493151617697 1108 74-75 -0.27692829511529027 1108 76 -0.1264836924896784 1109 1 -0.04243672089182837 1109 2 -0.13145353520386038 1109 3 0.09933215065596102 1109 4 0.09389688094225335 1109 5 0.07218149035694665 1109 6 -0.4232792705263506 1109 7 0.11317150761315986 1109 8 -0.06947936602136195 1109 9 -0.17169561727205007 1109 10-11 -0.18096696451786443 1109 12-13 -0.05913521448847803 1109 14-15 -0.033934477533705376 1109 16-17 0.04112429521225636 1109 18-19 -0.0018530954123363585 1109 20-21 0.11803578241971735 1109 22-23 -0.06381556126225263 1109 24-25 -0.09022521564107677 1109 26-27 -0.016736049414049603 1109 28-29 0.010565969357905658 1109 30-31 0.08902149549146543 1109 32-33 0.024734640113855733 1109 34-35 -0.26099201768448665 1109 36-37 -0.07998600030761338 1109 38-39 -0.0645675139117543 1109 40-41 0.16184639607072882 1109 42-43 0.0820741408343082 1109 44-45 0.10358935869309249 1109 46-47 -0.02081603424903733 1109 48-49 -0.16058892853343565 1109 50-51 -0.13980884370318591 1109 52-53 0.13399876261028254 1109 54-55 0.08334249788736514 1109 56-57 0.11557580110609678 1109 58-59 0.33759671348016695 1109 60-61 0.24643440308895492 1109 62-63 -0.26352231566104933 1109 64-65 -0.0398385045254841 1109 66-67 0.01907310560933695 1109 68-69 0.2933379962626219 1109 70-71 0.35309791357749276 1109 72-73 -0.21164947749650764 1109 74-75 0.14445483303475726 1109 76 0.6077144402046954 1110 1 -0.1396027127946624 1110 2 -0.13550211819980973 1110 3 0.050749154704540445 1110 4 -0.0639978558998493 1110 5 -0.00879016956207579 1110 6 -0.3018217806478134 1110 7 -0.1661807191074871 1110 8 0.03173520887742143 1110 9 0.05097644750527763 1110 10-11 -0.1445297175543061 1110 12-13 0.1918769312605093 1110 14-15 -0.28899520627864206 1110 16-17 -0.10462469264199115 1110 18-19 0.0629242325228887 1110 20-21 0.06945278646830388 1110 22-23 -0.22171029810435527 1110 24-25 -0.009253555722047224 1110 26-27 0.13306152143615435 1110 28-29 0.25752953211094365 1110 30-31 0.1841631958963319 1110 32-33 -0.09672284976468148 1110 34-35 -0.03629566140917717 1110 36-37 0.1993662264130336 1110 38-39 0.459723984063956 1110 40-41 0.3561783798763969 1110 42-43 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-0.26281099771089345 2115 76 0.362519675847949 2116 1 0.021723680110675048 2116 2 0.08628311411173684 2116 3 -0.25013767776123785 2116 4 0.052755566116729824 2116 5 -0.011566340759301852 2116 6 0.03484681391552158 2116 7 -0.21098503759601783 2116 8 -0.20522257018561874 2116 9 -0.14553405953154908 2116 10-11 -0.05835273664047946 2116 12-13 -0.2508259798634569 2116 14-15 0.22891495373677628 2116 16-17 -0.3865217429600989 2116 18-19 -0.38066551106685864 2116 20-21 -0.22093664865797535 2116 22-23 0.12499299859894109 2116 24-25 -0.26414077376715994 2116 26-27 -0.0956352204184796 2116 28-29 0.13713238539801154 2116 30-31 0.14331106557051498 2116 32-33 -0.06580902674559752 2116 34-35 -4.156462876636624E-5 2116 36-37 0.03591914717648592 2116 38-39 -0.8146349902950192 2116 40-41 -0.6638717454368859 2116 42-43 -0.12133823626227525 2116 44-45 -0.05758280247906811 2116 46-47 0.2779404724022072 2116 48-49 -0.16251682519817479 2116 50-51 -0.0790800987639173 2116 52-53 -0.5081739769308768 2116 54-55 -0.05751927192177675 2116 56-57 -0.39863279731302015 2116 58-59 -0.2287614897201351 2116 60-61 -0.25943604225517447 2116 62-63 -0.20177178548946273 2116 64-65 -0.44321232368877617 2116 66-67 -0.19124114154701743 2116 68-69 -0.13153187071250727 2116 70-71 -0.3120746331739994 2116 72-73 -0.58484207437332 2116 74-75 -1.002778635251346 2116 76 -1.143490495210763 2117 1 -0.07370300541501962 2117 2 0.08524586752994168 2117 3 -0.4016699738479872 2117 4 -0.10061548527412612 2117 5 -0.07960028701106836 2117 6 0.23102786059161673 2117 7 0.11607766867461322 2117 8 -0.8543031925335711 2117 9 -0.27830162200208264 2117 10-11 -0.17150690920059475 2117 12-13 -0.47718147255558563 2117 14-15 0.11189468028086225 2117 16-17 -0.03263206827834608 2117 18-19 0.2013476902532716 2117 20-21 -0.25181830825250984 2117 22-23 -0.08519087693147043 2117 24-25 -0.23358914717592683 2117 26-27 0.09931998938821351 2117 28-29 -0.47979359291410617 2117 30-31 -0.18191288539600947 2117 32-33 -0.4294582488106613 2117 34-35 -0.011663441102129468 2117 36-37 -0.19173290374288854 2117 38-39 -0.2489584226382533 2117 40-41 -0.15137763888336764 2117 42-43 -0.33406808067529425 2117 44-45 -0.388794495076894 2117 46-47 -0.2855673069471578 2117 48-49 -0.07953709865408598 2117 50-51 -0.11868405915995339 2117 52-53 0.20156199666648433 2117 54-55 -0.1474768391070711 2117 56-57 -0.2140029057524373 2117 58-59 -0.13418346048864294 2117 60-61 -0.44161426007696036 2117 62-63 -0.15139460491425893 2117 64-65 -0.36581958441485085 2117 66-67 -0.24383425800152025 2117 68-69 -0.2244597349274926 2117 70-71 0.034342151361592244 2117 72-73 -0.3086751719687868 2117 74-75 -0.25838919630744783 2117 76 -0.3292519284969515 2118 1 -0.20977815139115563 2118 2 -0.19137256354393628 2118 3 -0.46369079131434887 2118 4 0.06960538296654306 2118 5 -0.16101689020985077 2118 6 0.07550615457486032 2118 7 -0.12380555041652741 2118 8 -0.042218907181954535 2118 9 -0.09828131227880021 2118 10-11 -0.19095346924121515 2118 12-13 -0.28342671246419116 2118 14-15 -0.20991288509105743 2118 16-17 -0.2491591055974567 2118 18-19 -0.3063065367078792 2118 20-21 -0.173867051588374 2118 22-23 -0.2550436314376938 2118 24-25 -0.18941549904188548 2118 26-27 -0.24453631931958597 2118 28-29 -0.33356358529795926 2118 30-31 -0.2614508391913901 2118 32-33 -0.26635847729505 2118 34-35 -0.2742173888045869 2118 36-37 -0.11939220813486884 2118 38-39 0.22126244560242014 2118 40-41 -0.056912038477179294 2118 42-43 -0.2242686391926867 2118 44-45 0.04205089715463117 2118 46-47 0.084717029178762 2118 48-49 -0.2910882537696011 2118 50-51 -0.4021570218408428 2118 52-53 -0.4427893615462608 2118 54-55 -0.09250095690346427 2118 56-57 -0.37848627716650185 2118 58-59 -0.40788236884101536 2118 60-61 -0.24276937558851586 2118 62-63 -0.04975713347481303 2118 64-65 0.13371075323430404 2118 66-67 -0.2597393100451839 2118 68-69 -0.4806161197967498 2118 70-71 -0.602367673467036 2118 72-73 -0.01029994983119309 2118 74-75 -0.4412401737128846 2118 76 -0.9504502021704688 2119 1 -0.16215910377210463 2119 2 0.0822538100824346 2119 3 0.001876974253413266 2119 4 -0.056768243407077534 2119 5 -0.10826963746259821 2119 6 -0.1783399992712944 2119 7 -0.4487139753249565 2119 8 -0.4601676251306728 2119 9 -0.1942520082494923 2119 10-11 -0.08857251686026046 2119 12-13 -0.2867234157608962 2119 14-15 -1.2009385261167012 2119 16-17 -0.37443383087217796 2119 18-19 -0.3931197235210675 2119 20-21 0.010748333027009949 2119 22-23 -0.012919089313150778 2119 24-25 -0.11523967486606779 2119 26-27 -0.2119355867188517 2119 28-29 -0.07257457430895187 2119 30-31 -0.17500395274450398 2119 32-33 -0.3438310047675728 2119 34-35 0.011130596543395654 2119 36-37 -0.1217731605158221 2119 38-39 0.058625082965058084 2119 40-41 0.18960810804296813 2119 42-43 0.008332093408050412 2119 44-45 -0.2617952566915207 2119 46-47 -0.5429386484769125 2119 48-49 0.07740991472856251 2119 50-51 0.006084736400914892 2119 52-53 0.06343774468084007 2119 54-55 -0.3382885026910074 2119 56-57 0.052099803419572766 2119 58-59 -0.020702881661527783 2119 60-61 0.36254197972284175 2119 62-63 -0.07613075984843931 2119 64-65 0.2296814492049961 2119 66-67 0.3228614225555475 2119 68-69 0.3041824150017831 2119 70-71 1.1696103485109788 2119 72-73 0.8904326509014027 2119 74-75 1.6457561632834512 2119 76 0.31072195900168964 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 3.0 23 11.0 24 33.0 25 77.0 26 129.0 27 220.0 28 408.0 29 674.0 30 1086.0 31 1671.0 32 2594.0 33 4170.0 34 6584.0 35 9840.0 36 13844.0 37 47524.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.26876638647897 15.791783411538333 12.583690600771924 36.35575960121077 2 19.21817506667117 18.494638175291723 36.45703226096839 25.83015449706872 3 18.56890479244731 17.21972791412078 32.483768243144404 31.727599050287502 4 15.403571549134117 24.452846324365076 30.328911093857254 29.814671032643552 5 15.950443911825271 28.85595652027141 39.03610932946246 16.157490238440854 6 29.56824089390001 36.371513126061956 15.783906649112739 18.276339330925296 7 27.66881589755708 23.82495583386783 21.619462354701867 26.88676591387323 8 23.802450798366134 36.2803677322801 14.988353644127875 24.928827825225895 9 21.833260191967955 15.133511123113797 15.961696429576117 47.07153225534213 10-11 26.784930628228064 26.140161361104546 22.656381865442395 24.418526145224995 12-13 25.58991324308814 17.612440783625335 25.09030145495055 31.70734451833598 14-15 22.68732628925722 16.89115439579606 19.42297088973658 40.99854842521014 16-17 28.8823999369859 24.071385972611374 17.850431534055744 29.195782556346984 18-19 31.4192800639143 22.640628340591206 18.213325231520553 27.726766363973937 20-21 25.84928377724516 20.017103826981288 18.161563649866657 35.972048745906896 22-23 23.800200294815966 23.942544644364176 19.400465854234884 32.85678920658498 24-25 30.23438994475014 24.321754492567713 19.01619237304347 26.427663189638682 26-27 29.640819633392972 25.466698173716367 20.140318896353058 24.7521632965376 28-29 24.59294017036312 23.97236381640392 23.242075414373968 28.192620598858998 30-31 28.46886990964228 19.390338588259123 26.887328539760773 25.253462962337824 32-33 25.306912421654342 18.77820162261306 26.06195636273616 29.852929592996436 34-35 22.909563514836446 27.967007617954515 23.4856924236798 25.637736443529242 36-37 29.586807548188908 26.51712070575791 20.35636723716932 23.53970450888386 38-39 23.59709234941318 22.833046394130687 26.405720780024534 27.1641404764316 40-41 23.24095016259888 17.925260777098874 27.613115934690384 31.220673125611853 42-43 28.33102656719441 17.881938583758117 26.601514588889263 27.185520260158206 44-45 21.838886450843376 23.655605441717583 29.774161968740504 24.731346138698534 46-47 25.690060651070677 20.15719767297933 27.20296166267202 26.94978001327797 48-49 25.680496010982456 18.52501997321901 23.123361352102535 32.671122663696 50-51 23.30058850667837 19.22098819610888 26.979599185317717 30.498824111895033 52-53 19.08595798309872 19.127592298776854 35.892155869875886 25.894293848248545 54-55 20.294478389539663 20.467204537015157 30.201757643272682 29.036559430172503 56-57 24.115270791839674 21.735363287535588 24.31612823369229 29.833237686932453 58-59 20.86216791006988 22.464526437790454 30.024530488696843 26.648775163442824 60-61 24.393207980285588 26.435539952064275 24.642451248466845 24.528800819183292 62-63 19.77911307655088 34.93794236460408 20.383373279771348 24.899571279073694 64-65 18.845716729118138 36.764788621454045 19.595697037212076 24.793797612215734 66-67 19.07920647244821 36.18247082784773 19.60638692907538 25.131935770628676 68-69 18.76301072364942 36.22016676231306 20.158885550641955 24.85793696339556 70-71 19.119715536351258 34.40569827498903 20.9488122967514 25.525773891908315 72-73 19.338577006605227 31.139092371918213 21.91709144921176 27.605239172264795 74-75 19.09777312673711 29.954764878641598 23.030528080658048 27.91693391396325 76 19.191169024069136 30.987183382281785 22.587178881274685 27.234468712374394 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.0 15 2.0 16 5.5 17 7.0 18 5.5 19 3.5 20 7.0 21 11.0 22 14.5 23 30.0 24 43.0 25 44.0 26 82.0 27 146.0 28 195.0 29 218.0 30 272.0 31 372.5 32 551.5 33 684.0 34 792.0 35 1013.0 36 1205.0 37 1284.0 38 1625.5 39 3501.5 40 6127.5 41 6841.5 42 6464.0 43 5795.0 44 4693.0 45 3980.0 46 3700.0 47 3562.0 48 3238.5 49 2852.0 50 2651.0 51 2485.0 52 2077.0 53 1740.5 54 1646.0 55 1664.0 56 1768.5 57 1855.5 58 1856.0 59 1892.5 60 2128.5 61 2377.5 62 2427.0 63 2610.5 64 2860.5 65 2997.5 66 3028.0 67 2988.0 68 2955.5 69 2748.0 70 2312.5 71 2052.0 72 1771.5 73 1242.0 74 775.0 75 557.0 76 395.5 77 155.5 78 47.0 79 17.0 80 14.0 81 6.5 82 1.0 83 0.0 84 1.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 88869.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.1315194274719 #Duplication Level Percentage of deduplicated Percentage of total 1 65.54215177444242 31.54643351449887 2 19.645111516341704 18.91098133207305 3 6.833590498901201 9.867332815717516 4 3.0368915696451113 5.846808223339972 5 1.5991022583812595 3.848361070789589 6 1.0146350586805069 2.9301556223204943 7 0.6055080188899798 2.040081468228516 8 0.418478514985739 1.6113605419212549 9 0.3623696638144667 1.569726226243122 >10 0.8954037499415533 5.9255758475958995 >50 0.025716556786833124 0.876571132790962 >100 0.011689343994015056 1.3998132082053358 >500 0.0 0.0 >1k 0.0070136063964090336 6.408308859107226 >5k 0.002337868798803011 7.2184901371681915 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT 6415 7.2184901371681915 No Hit CACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTTCTG 2235 2.514937717314249 RNA PCR Primer, Index 47 (96% over 25bp) CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 1776 1.998447152550383 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG 1684 1.894923989242593 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 431 0.48498351506149506 No Hit ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT 295 0.33194927364997917 Illumina PCR Primer Index 9 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT 232 0.2610584118196447 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT 183 0.20592107484049557 No Hit CTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG 103 0.11590093283372153 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 100 0.11252517750846751 No Hit CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG 94 0.10577366685795948 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.013503021301016103 0.0 2 0.0 0.0 0.0 0.015753524851185453 0.0 3 0.0 0.0 0.0 0.021379783726608828 0.0 4 0.0 0.0 0.0 0.08214337958118129 0.0 5 0.0 0.0 0.0 0.09227064555694336 0.0 6 0.0 0.0 0.0 0.09339589733202804 0.0 7 0.0 0.0 0.0 0.09677165265728206 0.0 8 0.0 0.0 0.0 0.09677165265728206 0.0 9 0.0 0.0 0.0 0.10014740798253609 0.0 10 0.0 0.0 0.0 0.10352316330779011 0.0 11 0.0 0.0 0.0 0.10577366685795947 0.0 12 0.0 0.0 0.0 0.10577366685795947 0.0 13 0.0 0.0 0.0 0.10577366685795947 0.0 14 0.0 0.0 0.0 0.11252517750846752 0.0 15 0.0 0.0 0.0 0.11927668815897557 0.0 16 0.0 0.0011252517750846751 0.0 0.12040193993406025 0.0 17 0.0 0.0011252517750846751 0.0 0.12377769525931427 0.0 18 0.0 0.0011252517750846751 0.0 0.1305292059098223 0.0 19 0.0 0.0011252517750846751 0.0 0.13728071656033036 0.0 20 0.0 0.0011252517750846751 0.0 0.1451574789859231 0.0 21 0.0 0.0011252517750846751 0.0 0.14628273076100778 0.0011252517750846751 22 0.0 0.0011252517750846751 0.0 0.15303424141151584 0.0011252517750846751 23 0.0 0.0011252517750846751 0.0 0.1586605002869392 0.0011252517750846751 24 0.0 0.0011252517750846751 0.0 0.1676625144876166 0.0011252517750846751 25 0.0 0.0011252517750846751 0.0 0.17891503223846336 0.0011252517750846751 26 0.0 0.0011252517750846751 0.0 0.19241805353947947 0.0011252517750846751 27 0.0 0.0011252517750846751 0.0 0.27343618134557607 0.0011252517750846751 28 0.0 0.0011252517750846751 0.0 0.40058963193014435 0.0011252517750846751 29 0.0 0.0011252517750846751 0.0 0.5288683342897973 0.0011252517750846751 30 0.0 0.0011252517750846751 0.0 0.6368925046979261 0.0011252517750846751 31 0.0 0.0011252517750846751 0.0 0.7719227177080872 0.0011252517750846751 32 0.0 0.0011252517750846751 0.0 0.9654660230226513 0.0011252517750846751 33 0.0 0.0011252517750846751 0.0 1.1871406227143324 0.0011252517750846751 34 0.0 0.0011252517750846751 0.0 1.510087882163634 0.0011252517750846751 35 0.0 0.0011252517750846751 0.0 1.984944131249367 0.0011252517750846751 36 0.0 0.0011252517750846751 0.0 2.601582103995769 0.0011252517750846751 37 0.0 0.0011252517750846751 0.0 3.4702764743611385 0.0011252517750846751 38 0.0 0.0011252517750846751 0.0 4.208441638816685 0.0011252517750846751 39 0.0 0.0011252517750846751 0.0 4.996117881375958 0.0011252517750846751 40 0.0 0.0011252517750846751 0.0 5.71402851387998 0.0011252517750846751 41 0.0 0.0011252517750846751 0.0 6.474698713837221 0.0011252517750846751 42 0.0 0.0011252517750846751 0.0 7.270251718822086 0.0011252517750846751 43 0.0 0.0011252517750846751 0.0 8.011792638602888 0.0011252517750846751 44 0.0 0.0011252517750846751 0.0 8.780339600985721 0.0011252517750846751 45 0.0 0.0011252517750846751 0.0 9.608524907448041 0.0011252517750846751 46 0.0 0.0011252517750846751 0.0 10.459215249412056 0.0011252517750846751 47 0.0 0.0011252517750846751 0.0 11.275022786348446 0.0011252517750846751 48 0.0 0.0011252517750846751 0.0 11.97155363512586 0.0011252517750846751 49 0.0 0.0011252517750846751 0.0 12.648955203726834 0.0011252517750846751 50 0.0 0.0011252517750846751 0.0 13.262217421147982 0.0011252517750846751 51 0.0 0.0011252517750846751 0.0 13.94074424152404 0.0011252517750846751 52 0.0 0.0011252517750846751 0.0 14.668782140003826 0.0011252517750846751 53 0.0 0.0011252517750846751 0.0 15.323678673103107 0.0011252517750846751 54 0.0 0.0011252517750846751 0.0 15.941441897624593 0.0011252517750846751 55 0.0 0.0011252517750846751 0.0 16.509694044042355 0.0011252517750846751 56 0.0 0.0011252517750846751 0.0 17.120705757913335 0.0011252517750846751 57 0.0 0.0011252517750846751 0.0 17.6810811419055 0.0011252517750846751 58 0.0 0.0011252517750846751 0.0 18.240331274122585 0.0011252517750846751 59 0.0 0.0011252517750846751 0.0 18.824336945391533 0.0011252517750846751 60 0.0 0.0011252517750846751 0.0 19.39146384003421 0.0011252517750846751 61 0.0 0.0011252517750846751 0.0 19.987847280829087 0.0011252517750846751 62 0.0 0.0011252517750846751 0.0 20.52796813286973 0.0011252517750846751 63 0.0 0.0011252517750846751 0.0 21.116474811239016 0.0011252517750846751 64 0.0 0.0011252517750846751 0.0 21.65884616682983 0.0011252517750846751 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACTTGT 15 0.0022181359 70.0 16 TCTTTAT 35 2.9445982E-7 60.0 1 CTTTATA 45 1.6681624E-6 46.666664 2 ACTCGTA 40 4.5604946E-5 43.750004 38 AACTCGT 40 4.5604946E-5 43.750004 37 TAAGAAC 45 9.133527E-5 38.888885 33 CTACTCT 40 0.0024074786 35.000004 28 TCTGCTT 1590 0.0 34.339622 53 TTCTGCT 1595 0.0 34.231976 52 CCGTCTT 1585 0.0 34.227127 47 TCTTCTG 1590 0.0 34.119495 50 GTCTTCT 1590 0.0 34.119495 49 GTATGCC 1610 0.0 33.913044 42 CTTGAAA 1600 0.0 33.90625 57 GCTTGAA 1625 0.0 33.815384 56 TATGCCG 1615 0.0 33.808052 43 TCGTATG 1615 0.0 33.808052 40 TGCCGTC 1605 0.0 33.80062 45 CTGCTTG 1620 0.0 33.703705 54 CGTATGC 1620 0.0 33.703705 41 >>END_MODULE