FastQCFastQC Report
Wed 25 May 2016
SRR1780341_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780341_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533383
Sequences flagged as poor quality0
Sequence length50
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATTC207.8052806E-444.0541121
GGATTCA250.002353683635.19369522
CGGAATA300.00570777529.3694120
TTCGGAA300.00570777529.3694118
ATGTTAC300.00570777529.3694117
GATTCAT300.00574719829.3280823
CCAATGC551.5827974E-424.0204914
CAATGTG551.5939234E-423.99570329
ACAAAGT602.886127E-421.98162541
TCTTATC500.002591105421.9816252
AGATTTC550.0044631520.01331740
TAGAGAG708.1660604E-418.8396269
GGTATAT951.6059012E-518.5108411
GTCCAAT600.007394417718.33865212
TTCTCTC600.007438568418.31973832
CGTGATA750.001291135617.59684829
GCCTTTT750.001296016517.58694831
TCTAATA750.001296831517.58532
CTGGATA750.001296831517.585344
AGTATAG1307.3692354E-716.9073545
Produced by FastQC (version 0.11.5)